Restriction map for
pFN21AB7229
(Circular) (Six-base) MAPSORT of: pFN21AB7229.seq Check: 6822 from: 1 to: 5824
pFN21AB7229 5824 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 2702
Size: 5824
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3167 1937
Size: 637 593 4594
Fragments arranged by size:
4594 637 593
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2314 2723 2740 3887 5271 5539 1632
Size: 682 409 17 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 682 409 268 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2145 2549 3008 1166
Size: 979 404 459 3982
Fragments arranged by size:
3982 979 459 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2174 2213 2489 1184
Size: 990 39 276 4519
Fragments arranged by size:
4519 990 276 39
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2075 2075
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 3149 1870
Size: 73 675 531 4545
Fragments arranged by size:
4545 675 531 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3145 4600 618
Size: 325 217 627 246 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2075 3143 1391
Size: 684 1068 4072
Fragments arranged by size:
4072 1068 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2644 928
Size: 971 745 4108
Fragments arranged by size:
4108 971 745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2718 3241 2718
Size: 523 5301
Bme1580I G_kGCm'C
Cuts at: 1790 2075 3885 5041 1790
Size: 285 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2777 3555 4513
Size: 416 53 83 399 24 1360 778 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1360 958 778 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2877 2877
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 2618 2656 4484 1239
Size: 1190 189 38 1828 2579
Fragments arranged by size:
2579 1828 1190 189 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2101 2585 4487 882
Size: 617 602 484 1902 2219
Fragments arranged by size:
2219 1902 617 602 484
BsaAI yAC'GTr
Cuts at: 493 1204 2473 3615 493
Size: 711 1269 1142 2702
Fragments arranged by size:
2702 1269 1142 711
BsaBI GATnn'nnATC
Cuts at: 2261 2687 5818 2261
Size: 426 3131 2267
Fragments arranged by size:
3131 2267 426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 2399 1186
Size: 1180 33 4611
Fragments arranged by size:
4611 1180 33
BseYI C'CCAG_C
Cuts at: 3019 5027 5523 3019
Size: 2008 496 3320
Fragments arranged by size:
3320 2008 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3885 3970 5041 3885
Size: 85 1071 4668
Fragments arranged by size:
4668 1071 85
BsmI GAATG_Cn'
Cuts at: 2533 2600 3335 3428 2533
Size: 67 735 93 4929
Fragments arranged by size:
4929 735 93 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 3143 3885 3970 5041 1391
Size: 399 285 1068 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 399 285 85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 2868 3713 5443 2868
Size: 845 1730 3249
Fragments arranged by size:
3249 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2702 3179 844
Size: 1308 550 477 3489
Fragments arranged by size:
3489 1308 550 477
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2296 4319 4493 65
Size: 1183 1048 2023 174 1396
Fragments arranged by size:
2023 1396 1183 1048 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 96
Size: 2088 3736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 2141 3884 4896 2141
Size: 1743 1012 3069
Fragments arranged by size:
3069 1743 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2707 2966 1184
Size: 1523 259 4042
Fragments arranged by size:
4042 1523 259
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2147 2011
Size: 136 5688
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2217 2337 2560 2934 513
Size: 552 939 96 117 120 223 374 3403
Fragments arranged by size:
3403 939 552 374 223 120 117 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2477 2497 2990 3093 3340 4134 4154 2477
Size: 20 493 103 247 794 20 4147
Fragments arranged by size:
4147 794 493 247 103 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2466 2501 529
Size: 35 1902 35 3852
Fragments arranged by size:
3852 1902 35 35
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2503 3109 2503
Size: 606 5218
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3271 4160
Size: 54 1425 50 275 18 675 766 889
Cuts at: 4160 5789 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 766 675 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 2599 3754 1353
Size: 1046 200 1155 3423
Fragments arranged by size:
3423 1155 1046 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2314 2429 2618 2656 2723 2740 3887
Size: 393 682 115 189 38 67 17 1147
Cuts at: 3887 4484 5271 5539 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1147 787 682 597 393 268 189
115 67 38 17
EcoO109I rG'GnC_Cy
Cuts at: 2409 2725 2409
Size: 316 5508
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 4971 1678
Size: 3293 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 2393 2425 4465
Size: 32 135 32 781 32 8 32 2040
Cuts at: 4465 4497 4539 4571 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2040 781 135 42 32 32 32
32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 3168 3414 669
Size: 988 281 637 593 246 3079
Fragments arranged by size:
3079 988 637 593 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 2793 4938 5122 1305
Size: 808 680 2145 184 2007
Fragments arranged by size:
2145 2007 808 680 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 10
Size: 54 1425 343 675 3327
Fragments arranged by size:
3327 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2145 2549 3008 3782 4141 4300
Size: 648 843 136 404 459 774 359 159
Cuts at: 4300 518
Size: 2042
Fragments arranged by size:
2042 843 774 648 459 404 359 159
136
MspA1I CmG'CkG
Cuts at: 1606 2775 3240 3917 5065 5310 1606
Size: 1169 465 677 1148 245 2120
Fragments arranged by size:
2120 1169 1148 677 465 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2100 2560 513
Size: 552 939 96 460 3777
Fragments arranged by size:
3777 939 552 460 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2161 2912 3182 4727 1132
Size: 97 932 751 270 1545 2229
Fragments arranged by size:
2229 1545 932 751 270 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 2775 1108
Size: 1395 272 4157
Fragments arranged by size:
4157 1395 272
PfoI T'CCnGG_A
Cuts at: 2849 2952 2849
Size: 103 5721
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 2473 2473
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2409 2725 2409
Size: 316 5508
PshAI GACnn'nnGTC
Cuts at: 1613 2805 1613
Size: 1192 4632
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2071 2071
Size: 5824
PstI C_TGCA'G
Cuts at: 830 3176 830
Size: 2346 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2775 2775
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 2573 3166 1936
Size: 637 593 4594
Fragments arranged by size:
4594 637 593
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SexAI A'CCwGG_T
Cuts at: 2318 2318
Size: 5824
SfcI C'TryA_G
Cuts at: 826 1045 3172 4307 4988 5179 826
Size: 219 2127 1135 681 191 1471
Fragments arranged by size:
2127 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2620 3151 1872
Size: 748 531 4545
Fragments arranged by size:
4545 748 531
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2161 3182 1132
Size: 1029 1021 3774
Fragments arranged by size:
3774 1029 1021
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 2747 3030 1517
Size: 564 666 283 4311
Fragments arranged by size:
4311 666 564 283
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2100 2560 3263 513
Size: 552 939 63 33 460 703 3074
Fragments arranged by size:
3074 939 703 552 460 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 4070 5782
Size: 274 80 33 51 1443 207 1886 1712
Cuts at: 5782 96
Size: 138
Fragments arranged by size:
1886 1712 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2934 2966 5784 5816 2934
Size: 32 2818 32 2942
Fragments arranged by size:
2942 2818 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2422
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2618 3149 1870
Size: 748 531 4545
Fragments arranged by size:
4545 748 531
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI