Restriction map for
pFN21AB7226
(Circular) (Six-base) MAPSORT of: pFN21AB7226.seq Check: 7573 from: 1 to: 6064
pFN21AB7226 6064 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2653 2922 3110 4127 5511 5779 1632
Size: 1021 269 188 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1021 1017 269 268 188
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3011 1184
Size: 1827 4237
AlwNI CAG_nnn'CTG
Cuts at: 2773 3807 5379 2773
Size: 1034 1572 3458
Fragments arranged by size:
3458 1572 1034
ApaLI G'TGCA_C
Cuts at: 3077 3107 4121 5277 3077
Size: 30 1014 1156 3864
Fragments arranged by size:
3864 1156 1014 30
ApoI r'AATT_y
Cuts at: 1493 3373 3599 3751 5860 1493
Size: 1880 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
BamHI G'GATC_C
Cuts at: 2013 3394 3761 2013
Size: 1381 367 4316
Fragments arranged by size:
4316 1381 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2606 3385 4840 618
Size: 325 217 627 819 779 1455 1842
Fragments arranged by size:
1842 1455 819 779 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3276 3383 1391
Size: 1885 107 4072
Fragments arranged by size:
4072 1885 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2444 2987 928
Size: 971 545 543 4005
Fragments arranged by size:
4005 971 545 543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4255 4289 1903
Size: 34 50 34 2234 34 3678
Fragments arranged by size:
3678 2234 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2836 3956 5166 2836
Size: 1120 1210 3734
Fragments arranged by size:
3734 1210 1120
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2604 2726 4675
Size: 107 122 71 631 605 932 122 1949
Cuts at: 4675 136
Size: 1525
Fragments arranged by size:
1949 1525 932 631 605 122 122 107
71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3315 3481 3315
Size: 166 5898
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2609 3081 3111 4125 5281 1790
Size: 281 538 472 30 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 538 472 281 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3239 3795 4753
Size: 416 53 83 399 24 1822 556 958
Cuts at: 4753 442
Size: 1753
Fragments arranged by size:
1822 1753 958 556 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3066 3066
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2274 3038 4724 1239
Size: 917 118 764 1686 2579
Fragments arranged by size:
2579 1686 917 764 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 1186
Size: 1207 4857
BseYI C'CCAG_C
Cuts at: 2779 3358 5267 5763 2779
Size: 579 1909 496 3080
Fragments arranged by size:
3080 1909 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 2702 2984 1163
Size: 573 747 219 282 4243
Fragments arranged by size:
4243 747 573 282 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3081 3111 4125 4210 5281 3081
Size: 30 1014 85 1071 3864
Fragments arranged by size:
3864 1071 1014 85 30
BsmI GAATG_Cn'
Cuts at: 3340 3575 3668 3340
Size: 235 93 5736
Fragments arranged by size:
5736 235 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2609 3081 3111 3276 3383 4125
Size: 399 281 538 472 30 165 107 742
Cuts at: 4125 4210 5281 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 472 399 281 165
107 85 30
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 2094 3953 5683 2094
Size: 1859 1730 2475
Fragments arranged by size:
2475 1859 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 3419 844
Size: 1610 965 3489
Fragments arranged by size:
3489 1610 965
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2361 4708 4895 1700
Size: 110 551 2347 187 2869
Fragments arranged by size:
2869 2347 551 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 3110 4124 5136 3110
Size: 1014 1012 4038
Fragments arranged by size:
4038 1014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2773 2804 794
Size: 1979 31 4054
Fragments arranged by size:
4054 1979 31
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2884 3139 2593
Size: 291 255 5518
Fragments arranged by size:
5518 291 255
BstYI r'GATC_y
Cuts at: 1972 2013 3394 3761 4146 4163 5615 5701 5713
Size: 41 1381 367 385 17 1452 86 12
Cuts at: 5713 6059 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1381 385 367 346 86 41
17 12
Bsu36I CC'TnA_GG
Cuts at: 2742 2742
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2115 513
Size: 552 1050 4462
Fragments arranged by size:
4462 1050 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 3580 4374 4394 2069
Size: 1511 794 20 3739
Fragments arranged by size:
3739 1511 794 20
ClaI AT'CG_AT
Cuts at: 1554 2535 3176 1554
Size: 981 641 4442
Fragments arranged by size:
4442 981 641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3129 3294 3511
Size: 54 1425 50 275 18 1299 165 217
Cuts at: 3511 4400 6029 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 275 217 165 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3994 1353
Size: 2641 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3055 4651 5025 5171 387
Size: 1064 138 1466 1596 374 146 1280
Fragments arranged by size:
1596 1466 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2274 2653 2922 3038 3110 4127
Size: 393 524 118 379 269 116 72 1017
Cuts at: 4127 4724 5511 5779 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 524 393 379 269
268 118 116 72
EcoO109I rG'GnC_Cy
Cuts at: 2728 2728
Size: 6064
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2115 5211 1678
Size: 437 3096 2531
Fragments arranged by size:
3096 2531 437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2247 2279 2872 2904 4705
Size: 32 135 32 675 32 593 32 1801
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1801 675 593 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2476 3408 3654 669
Size: 988 281 538 932 246 3079
Fragments arranged by size:
3079 988 932 538 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 748
Size: 1960 4104
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2683 5178 5362 1305
Size: 1378 2495 184 2007
Fragments arranged by size:
2495 2007 1378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3131 3296 10
Size: 54 1425 343 1299 165 2778
Fragments arranged by size:
2778 1425 1299 343 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2591 4022 4381 4540 518
Size: 648 1119 306 1431 359 159 2042
Fragments arranged by size:
2042 1431 1119 648 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2770 3121 3480 4157 5305 5550 1606
Size: 618 546 351 359 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 618 546 359 351 245
NcoI C'CATG_G
Cuts at: 513 1065 2115 513
Size: 552 1050 4462
Fragments arranged by size:
4462 1050 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2461 3422 4967 1132
Size: 97 1232 961 1545 2229
Fragments arranged by size:
2229 1545 1232 961 97
PasI CC'CwG_GG
Cuts at: 1348 2063 2513 1348
Size: 715 450 4899
Fragments arranged by size:
4899 715 450
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2133 2484 2838 3161 3282 2133
Size: 351 354 323 121 4915
Fragments arranged by size:
4915 354 351 323 121
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2670 1613
Size: 1057 5007
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2036 2800 2817 3126 3416 830
Size: 1206 764 17 309 290 3478
Fragments arranged by size:
3478 1206 764 309 290 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2224 2770 3121 2224
Size: 546 351 5167
Fragments arranged by size:
5167 546 351
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 3101 4312 6024 3101
Size: 1211 1712 3141
Fragments arranged by size:
3141 1712 1211
SexAI A'CCwGG_T
Cuts at: 2100 2100
Size: 6064
SfcI C'TryA_G
Cuts at: 826 1045 2032 2796 2813 2910 3122 3412 4547
Size: 219 987 764 17 97 212 290 1135
Cuts at: 4547 5228 5419 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 987 764 681 290 219 212
191 97 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 5069 5331 820
Size: 197 926 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2388 1517
Size: 871 5193
StyI C'CwwG_G
Cuts at: 513 1065 2115 2967 3503 513
Size: 552 1050 852 536 3074
Fragments arranged by size:
3074 1050 852 552 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 4108 4261 4446 4891 325
Size: 1255 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3099 4310 6022
Size: 274 80 33 51 1443 1122 1211 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1443 1211 1122 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 2881
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI