Restriction map for
pFN21AB7222
(Circular) (Six-base) MAPSORT of: pFN21AB7222.seq Check: 521 from: 1 to: 6070
pFN21AB7222 6070 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2887 2887
Size: 6070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3162 3880 278
Size: 53 83 186 936 1626 718 2468
Fragments arranged by size:
2468 1626 936 718 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2696 2717 2877 4133 5517 5785 1632
Size: 901 163 21 160 1256 1384 268 1917
Fragments arranged by size:
1917 1384 1256 901 268 163 160 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2421 3189 4969 5946 1203
Size: 1218 768 1780 977 1327
Fragments arranged by size:
1780 1327 1218 977 768
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 2437 2960 4799 2437
Size: 523 1839 3708
Fragments arranged by size:
3708 1839 523
AleI CACnn'nnGTG
Cuts at: 1166 2084 3086 1166
Size: 918 1002 4150
Fragments arranged by size:
4150 1002 918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 3151 3813 5385 3151
Size: 662 1572 3836
Fragments arranged by size:
3836 1572 662
ApaI G_GGCC'C
Cuts at: 3169 3169
Size: 6070
ApaLI G'TGCA_C
Cuts at: 3243 4127 5283 3243
Size: 884 1156 4030
Fragments arranged by size:
4030 1156 884
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 3395 1870
Size: 73 1452 4545
Fragments arranged by size:
4545 1452 73
BamHI G'GATC_C
Cuts at: 2619 3400 3767 2619
Size: 781 367 4922
Fragments arranged by size:
4922 781 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2814 3391 4846 618
Size: 325 217 627 1027 577 1455 1842
Fragments arranged by size:
1842 1455 1027 627 577 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2078 2466 3169 3389 1391
Size: 666 21 388 703 220 4072
Fragments arranged by size:
4072 703 666 388 220 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2480 2865 3275 928
Size: 971 581 385 410 3723
Fragments arranged by size:
3723 971 581 410 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 3078 1548
Size: 1530 4540
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 2534 3101
Size: 107 122 71 631 605 659 203 567
Cuts at: 3101 4681 136
Size: 1580 1525
Fragments arranged by size:
1580 1525 659 631 605 567 203 122
107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 2991 3487 2991
Size: 496 5574
Bme1580I G_kGCm'C
Cuts at: 1790 2429 3169 3247 3351 4131 5287 1790
Size: 639 740 78 104 780 1156 2573
Fragments arranged by size:
2573 1156 780 740 639 104 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 2247 2360 2378 2399 2450 2675 2822
Size: 847 161 113 18 21 51 225 147
Cuts at: 2822 2993 3143 3194 3227 4730 1239
Size: 171 150 51 33 1503 2579
Fragments arranged by size:
2579 1503 847 225 171 161 150 147
113 51 51 33 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2033 4195 5060 5358 2006
Size: 27 2162 865 298 2718
Fragments arranged by size:
2718 2162 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 2294 4733 882
Size: 617 550 245 2439 2219
Fragments arranged by size:
2439 2219 617 550 245
BsaAI yAC'GTr
Cuts at: 493 1204 3192 3861 493
Size: 711 1988 669 2702
Fragments arranged by size:
2702 1988 711 669
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3159 3877 4259 4894
Size: 53 83 186 936 1626 718 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1626 1451 936 718 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2448 4503 4901 5175 5322 5733 2448
Size: 2055 398 274 147 411 2785
Fragments arranged by size:
2785 2055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 3245 3275 1178
Size: 30 165 30 1395 30 417 30 3973
Fragments arranged by size:
3973 1395 417 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 2696 2726 2819 2897 1186
Size: 1288 222 30 93 78 4359
Fragments arranged by size:
4359 1288 222 93 78 30
BseYI C'CCAG_C
Cuts at: 3054 3096 5273 5769 3054
Size: 42 2177 496 3355
Fragments arranged by size:
3355 2177 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3330 1163
Size: 573 1594 3903
Fragments arranged by size:
3903 1594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 3520 4281 5309 1540
Size: 275 186 322 1197 761 1028 2301
Fragments arranged by size:
2301 1197 1028 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 3247 4131 4216 5287 2466
Size: 781 884 85 1071 3249
Fragments arranged by size:
3249 1071 884 781 85
BsmI GAATG_Cn'
Cuts at: 2503 3581 3674 2503
Size: 1078 93 4899
Fragments arranged by size:
4899 1078 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2558 2947 3026 2558
Size: 389 79 5602
Fragments arranged by size:
5602 389 79
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2078 2429 2466 3169 3247 3351
Size: 399 267 21 351 37 703 78 104
Cuts at: 3351 3389 4131 4216 5287 1391
Size: 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 399 351 267 104
85 78 38 37 21
BspEI T'CCGG_A
Cuts at: 2448 5733 2448
Size: 3285 2785
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2401 2887 3425 844
Size: 1557 486 538 3489
Fragments arranged by size:
3489 1557 538 486
BsrBI CCG'CTC
Cuts at: 1394 1823 3127 3957 1394
Size: 429 1304 830 3507
Fragments arranged by size:
3507 1304 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2100 2296 4565 4739 65
Size: 1183 852 196 2269 174 1396
Fragments arranged by size:
2269 1396 1183 852 196 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2330 4714 4901 1700
Size: 110 217 303 2384 187 2869
Fragments arranged by size:
2869 2384 303 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2082 4130 5142 2082
Size: 2048 1012 3010
Fragments arranged by size:
3010 2048 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2112 3324 794
Size: 1318 1212 3540
Fragments arranged by size:
3540 1318 1212
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 2744 1184
Size: 1560 4510
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2619 3108 3400 3767 4152 4169 5621 5707
Size: 647 489 292 367 385 17 1452 86
Cuts at: 5707 5719 6065 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 647 489 385 367 346 292
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3077 3586 4380 4400 2726
Size: 351 509 794 20 4396
Fragments arranged by size:
4396 794 509 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2572 5077 809
Size: 507 1256 2505 1802
Fragments arranged by size:
2505 1802 1256 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2328 3102
Size: 54 1425 50 275 18 309 189 774
Cuts at: 3102 3517 4406 6035 8
Size: 415 889 1629 43
Fragments arranged by size:
1629 1425 889 774 415 309 275 189
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2041 4000 1353
Size: 688 1959 3423
Fragments arranged by size:
3423 1959 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 2464
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2247 2360 2378 2399 2450 2533
Size: 393 454 161 113 18 21 51 83
Cuts at: 2533 2675 2696 2717 2822 2877 2993 3143 3194
Size: 142 21 21 105 55 116 150 51
Cuts at: 3194 3227 4133 4730 5517 5785 1239
Size: 33 906 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 454 393 268 161
150 142 116 113 105 83 55 51
51 33 21 21 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2226 2342 2678 2226
Size: 116 336 5618
Fragments arranged by size:
5618 336 116
EcoO109I rG'GnC_Cy
Cuts at: 2413 3199 2413
Size: 786 5284
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 6070
FspI TGC'GCA
Cuts at: 2615 4576 2615
Size: 1961 4109
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2946 2978 4711
Size: 32 135 32 851 32 491 32 1733
Cuts at: 4711 4743 4785 4817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1733 851 491 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2785 2965 3414 3660 669
Size: 988 281 847 180 449 246 3079
Fragments arranged by size:
3079 988 847 449 281 246 180
HindIII A'AGCT_T
Cuts at: 748 2117 748
Size: 1369 4701
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2847 5184 5368 1305
Size: 1542 2337 184 2007
Fragments arranged by size:
2337 2007 1542 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 10
Size: 54 1425 343 309 3939
Fragments arranged by size:
3939 1425 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 3086 3344 4028 4387 4546 518
Size: 648 918 1002 258 684 359 159 2042
Fragments arranged by size:
2042 1002 918 684 648 359 258 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2356 2587 3058 3100 3486 4163 5311
Size: 593 157 231 471 42 386 677 1148
Cuts at: 5311 5556 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 593 471 386 245 231
157 42
NaeI GCC'GGC
Cuts at: 2029 2332 2029
Size: 303 5767
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NgoMIV G'CCGG_C
Cuts at: 2027 2330 2027
Size: 303 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2425 3428 4973 1132
Size: 97 1196 1003 1545 2229
Fragments arranged by size:
2229 1545 1196 1003 97
PasI CC'CwG_GG
Cuts at: 1348 3356 1348
Size: 2008 4062
PciI A'CATG_T
Cuts at: 2421 4969 2421
Size: 2548 3522
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2899 1108
Size: 1791 4279
PfoI T'CCnGG_A
Cuts at: 2622 2622
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2413
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PspOMI G'GGCC_C
Cuts at: 3165 3165
Size: 6070
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 6038
PstI C_TGCA'G
Cuts at: 830 2542 2988 3329 3422 830
Size: 1712 446 341 93 3478
Fragments arranged by size:
3478 1712 446 341 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2199 2356 2587 3058 3100 2199
Size: 157 231 471 42 5169
Fragments arranged by size:
5169 471 231 157 42
SacI G_AGCT'C
Cuts at: 2466 2466
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SexAI A'CCwGG_T
Cuts at: 3144 3144
Size: 6070
SfcI C'TryA_G
Cuts at: 826 1045 2185 2245 2538 2984 3046 3325 3418
Size: 219 1140 60 293 446 62 279 93
Cuts at: 3418 4553 5234 5425 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1140 1135 681 446 293 279 219
191 93 62 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 3101 3101
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2048 4174 5075 5337 820
Size: 197 926 78 27 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 197 78 27
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2004 3509 513
Size: 552 939 1505 3074
Fragments arranged by size:
3074 1505 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2888 4097 4114 4267 4452 4897 325
Size: 1255 1308 1209 17 153 185 445 1498
Fragments arranged by size:
1498 1308 1255 1209 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 4316 6028
Size: 274 80 33 51 1443 120 2219 1712
Cuts at: 6028 96
Size: 138
Fragments arranged by size:
2219 1712 1443 274 138 120 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 6030 6062 2798
Size: 32 3200 32 2806
Fragments arranged by size:
3200 2806 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2509
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3160 3878 276
Size: 53 83 186 936 1626 718 2468
Fragments arranged by size:
2468 1626 936 718 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
RsrII SacII SanDI SapI SfoI SrfI SwaI