Restriction map for
pFN21AB7220
(Circular) (Six-base) MAPSORT of: pFN21AB7220.seq Check: 5786 from: 1 to: 5662
pFN21AB7220 5662 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3725 5109 5377 1632
Size: 947 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2344 1184
Size: 1160 4502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2421
Size: 32 5630
AlwNI CAG_nnn'CTG
Cuts at: 3405 4977 3405
Size: 1572 4090
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2523 2971 3197 3349 5458 1493
Size: 1030 448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1030 448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2981 1391
Size: 1590 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 2277 1548
Size: 729 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2952 3079 2952
Size: 127 5535
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2912 3393 4351
Size: 416 53 83 399 24 1495 481 958
Cuts at: 4351 442
Size: 1753
Fragments arranged by size:
1753 1495 958 481 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4322 1239
Size: 3083 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3787 4652 4950 2498
Size: 1289 865 298 3210
Fragments arranged by size:
3210 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2474 4325 882
Size: 617 975 1851 2219
Fragments arranged by size:
2219 1851 975 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2072 2662 2903 2906 1186
Size: 847 39 590 241 3 3942
Fragments arranged by size:
3942 847 590 241 39 3
BseYI C'CCAG_C
Cuts at: 4865 5361 4865
Size: 496 5166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2494 1163
Size: 573 642 116 4331
Fragments arranged by size:
4331 642 573 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3723 3808 4879 3723
Size: 85 1071 4506
Fragments arranged by size:
4506 1071 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
BsmBI CGTCTCn'nnnn_
Cuts at: 2934 2934
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2981 3723 3808 4879 1391
Size: 399 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 2409 3551 5281 2409
Size: 1142 1730 2790
Fragments arranged by size:
2790 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3017 844
Size: 1295 878 3489
Fragments arranged by size:
3489 1295 878
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 2946 4157 4331 65
Size: 1183 1068 630 1211 174 1396
Fragments arranged by size:
1396 1211 1183 1068 630 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 2926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 2113 2842 1184
Size: 929 729 4004
Fragments arranged by size:
4004 929 729
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2620 2110
Size: 510 5152
BstYI r'GATC_y
Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657
Size: 1020 367 385 17 1452 86 12 346
Cuts at: 5657 1972
Size: 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2876 2918 2876
Size: 42 5620
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 2838 513
Size: 552 939 82 752 3337
Fragments arranged by size:
3337 939 752 552 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3109 3998
Size: 54 1425 50 275 18 546 733 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 733 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3592 1353
Size: 2239 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 3725 4322 5109 5377 1239
Size: 393 947 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 947 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2445 2445
Size: 5662
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 4303 4335 4377
Size: 32 135 32 1329 32 1370 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1370 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2011 2220 4776 4960 1305
Size: 706 209 2556 184 2007
Fragments arranged by size:
2556 2007 706 209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3294
Fragments arranged by size:
3294 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2326 2516 2597 2849 3620 3979
Size: 648 1011 149 190 81 252 771 359
Cuts at: 3979 4138 518
Size: 159 2042
Fragments arranged by size:
2042 1011 771 648 359 252 190 159
149 81
MspA1I CmG'CkG
Cuts at: 1606 3078 3755 4903 5148 1606
Size: 1472 677 1148 245 2120
Fragments arranged by size:
2120 1472 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2086 2838 513
Size: 552 939 82 752 3337
Fragments arranged by size:
3337 939 752 552 82
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2074 3020 4565 1132
Size: 97 845 946 1545 2229
Fragments arranged by size:
2229 1545 946 845 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2445 2445
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 2615 1613
Size: 1002 4660
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 2791 3014 830
Size: 1961 223 3478
Fragments arranged by size:
3478 1961 223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5662
SfcI C'TryA_G
Cuts at: 826 1045 2787 2869 3010 4145 4826 5017 826
Size: 219 1742 82 141 1135 681 191 1471
Fragments arranged by size:
1742 1471 1135 681 219 191 141 82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 3766 4667 4929 820
Size: 197 926 534 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 2767 2838 3101 513
Size: 552 939 82 681 71 263 3074
Fragments arranged by size:
3074 939 681 552 263 82 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3689 3706 3859 4044 4489 325
Size: 1255 1032 1077 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1077 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2832 3908
Size: 274 80 33 51 1443 204 651 1076
Cuts at: 3908 5620 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 651 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2027 2027
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 2861 3791 2861
Size: 930 4732
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI