Restriction map for
pFN21AB7218
(Circular) (Six-base) MAPSORT of: pFN21AB7218.seq Check: 84 from: 1 to: 5455
pFN21AB7218 5455 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2703 2798 1937
Size: 766 95 4594
Fragments arranged by size:
4594 766 95
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 2754 3582 3955 1563
Size: 1191 828 373 3063
Fragments arranged by size:
3063 1191 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2491 1166
Size: 1325 4130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2249 2281 2249
Size: 32 5423
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaI G_GGCC'C
Cuts at: 2624 2624
Size: 5455
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2764 2990 3142 5251 1493
Size: 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2780 1870
Size: 73 837 4545
Fragments arranged by size:
4545 837 73
BamHI G'GATC_C
Cuts at: 2733 2785 3152 2733
Size: 52 367 5036
Fragments arranged by size:
5036 367 52
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2691 2776 4231 618
Size: 325 217 627 904 85 1455 1842
Fragments arranged by size:
1842 1455 904 627 325 217 85
BanII G_rGCy'C
Cuts at: 1391 2624 2676 2774 1391
Size: 1233 52 98 4072
Fragments arranged by size:
4072 1233 98 52
BbeI G_GCGC'C
Cuts at: 2695 2695
Size: 5455
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BbvCI CC'TCA_GC
Cuts at: 2312 2399 2312
Size: 87 5368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2583 2617 3646 3680 1903
Size: 34 646 34 1029 34 3678
Fragments arranged by size:
3678 1029 646 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 4066 136
Size: 107 122 71 631 605 406 1988 1525
Fragments arranged by size:
1988 1525 631 605 406 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2670 2872 2670
Size: 202 5253
Bme1580I G_kGCm'C
Cuts at: 1790 2624 3516 4672 1790
Size: 834 892 1156 2573
Fragments arranged by size:
2573 1156 892 834
BmgBI CAC'GTC
Cuts at: 1318 2369 1318
Size: 1051 4404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2542 3186 4144
Size: 416 53 83 399 24 1125 644 958
Cuts at: 4144 442
Size: 1753
Fragments arranged by size:
1753 1125 958 644 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 2665 4115 1239
Size: 1295 131 1450 2579
Fragments arranged by size:
2579 1450 1295 131
Bpu10I CC'TnA_GC
Cuts at: 2312 2399 2312
Size: 87 5368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2692 3262 3644 4279
Size: 53 83 186 936 1159 570 382 635
Cuts at: 4279 275
Size: 1451
Fragments arranged by size:
1451 1159 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2254 3888 4286 4560 4707 5118 2254
Size: 1634 398 274 147 411 2591
Fragments arranged by size:
2591 1634 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 1178
Size: 30 165 30 846 30 4354
Fragments arranged by size:
4354 846 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 1186
Size: 823 4632
BseYI C'CCAG_C
Cuts at: 2011 2055 2103 2445 4658 5154 2011
Size: 44 48 342 2213 496 2312
Fragments arranged by size:
2312 2213 496 342 48 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 1163
Size: 573 669 4213
Fragments arranged by size:
4213 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2029 2232 3516 3601 4672 2029
Size: 203 1284 85 1071 2812
Fragments arranged by size:
2812 1284 1071 203 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2232 2624 2676 2774 3516 3601
Size: 399 239 203 392 52 98 742 85
Cuts at: 3601 4672 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 239 203 98
85 52
BspEI T'CCGG_A
Cuts at: 2254 5118 2254
Size: 2864 2591
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2810 844
Size: 1966 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2641 3950 4124 65
Size: 1183 1393 1309 174 1396
Fragments arranged by size:
1396 1393 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 4099 4286 1700
Size: 110 307 1982 187 2869
Fragments arranged by size:
2869 1982 307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2733 2785 3152 3537 3554 5006 5092 5104
Size: 761 52 367 385 17 1452 86 12
Cuts at: 5104 5450 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 761 385 367 346 86 52
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4903
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 2435 2486 2498 2971 3765 3785 2191
Size: 244 51 12 473 794 20 3861
Fragments arranged by size:
3861 794 473 244 51 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420
Size: 54 1425 50 275 18 1072 889 1629
Cuts at: 5420 8
Size: 43
Fragments arranged by size:
1629 1425 1072 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2147 3385 1353
Size: 776 18 1238 3423
Fragments arranged by size:
3423 1238 776 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 2339 4042 4416 4562 387
Size: 1064 138 618 132 1703 374 146 1280
Fragments arranged by size:
1703 1280 1064 618 374 146 138 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 2665 3518 4115 4902 5170 1239
Size: 393 902 131 853 597 787 268 1524
Fragments arranged by size:
1524 902 853 787 597 393 268 131
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 5455
EcoO109I rG'GnC_Cy
Cuts at: 2308 2620 2621 2308
Size: 312 1 5142
Fragments arranged by size:
5142 312 1
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FseI GG_CCGG'CC
Cuts at: 2121 2121
Size: 5455
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 2319 2474 2695 4602 1678
Size: 641 155 221 1907 2531
Fragments arranged by size:
2531 1907 641 221 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373
Size: 32 135 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KasI G'GCGC_C
Cuts at: 2691 2691
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2491 3413 3772 3931 518
Size: 648 1165 160 922 359 159 2042
Fragments arranged by size:
2042 1165 922 648 359 160 159
MspA1I CmG'CkG
Cuts at: 1606 2172 2186 2263 2871 3548 4696 4941 1606
Size: 566 14 77 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 566 245 77 14
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 5455
NarI GG'CG_CC
Cuts at: 2692 2692
Size: 5455
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4903
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2428 2566 2813 4358 1132
Size: 97 1199 138 247 1545 2229
Fragments arranged by size:
2229 1545 1199 247 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5455
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5455
PfoI T'CCnGG_A
Cuts at: 2511 2511
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2249 2281 3524 3556 5037 5069 2249
Size: 32 1243 32 1481 32 2635
Fragments arranged by size:
2635 1481 1243 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 5455
PshAI GACnn'nnGTC
Cuts at: 1613 2411 1613
Size: 798 4657
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PspOMI G'GGCC_C
Cuts at: 2620 2620
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2493 2807 830
Size: 1663 314 3478
Fragments arranged by size:
3478 1663 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
PvuII CAG'CTG
Cuts at: 2186 2263 2186
Size: 77 5378
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SapI GCTCTTCn'nnn_
Cuts at: 2147 2147
Size: 5455
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2489 2803 3938 4619 4810 826
Size: 219 1444 314 1135 681 191 1471
Fragments arranged by size:
1471 1444 1135 681 314 219 191
SfoI GGC'GCC
Cuts at: 2693 2693
Size: 5455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2782 1872
Size: 910 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3559 4460 4722 820
Size: 197 926 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2428 2566 2813 1132
Size: 1296 138 247 3774
Fragments arranged by size:
3774 1296 247 138
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2108 2221 2466 2643 2894 513
Size: 552 1043 113 245 177 251 3074
Fragments arranged by size:
3074 1043 552 251 245 177 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3482 3499 3652 3837 4282 325
Size: 1255 1902 17 153 185 445 1498
Fragments arranged by size:
1902 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2612 2612
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2780 1870
Size: 910 4545
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI