Restriction map for
pFN21AB7206
(Circular) (Six-base) MAPSORT of: pFN21AB7206.seq Check: 5602 from: 1 to: 6430
pFN21AB7206 6430 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2865 4240 278
Size: 53 83 186 936 1329 1375 2468
Fragments arranged by size:
2468 1375 1329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2628 3773 1937
Size: 691 1145 4594
Fragments arranged by size:
4594 1145 691
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4493 5877 6145 1632
Size: 2861 1384 268 1917
Fragments arranged by size:
2861 1917 1384 268
AfeI AGC'GCT
Cuts at: 3375 3375
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 3699 5329 6306 1203
Size: 2496 1630 977 1327
Fragments arranged by size:
2496 1630 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 2648 1166
Size: 1482 4948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2037 1184
Size: 853 5577
AlwNI CAG_nnn'CTG
Cuts at: 2315 3337 4173 5745 2315
Size: 1022 836 1572 3000
Fragments arranged by size:
3000 1572 1022 836
ApaI G_GGCC'C
Cuts at: 2390 2390
Size: 6430
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 3008 3464 3739 3965 4117 6226 1493
Size: 1515 456 275 226 152 2109 1697
Fragments arranged by size:
2109 1697 1515 456 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 2592 3302 3755 1870
Size: 73 351 298 710 453 4545
Fragments arranged by size:
4545 710 453 351 298 73
AvrII C'CTAG_G
Cuts at: 2782 2782
Size: 6430
BamHI G'GATC_C
Cuts at: 3327 3760 4127 3327
Size: 433 367 5630
Fragments arranged by size:
5630 433 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 3751 5206 618
Size: 325 217 627 260 1704 1455 1842
Fragments arranged by size:
1842 1704 1455 627 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2390 3173 3274 3749 1391
Size: 999 783 101 475 4072
Fragments arranged by size:
4072 999 783 475 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3530 928
Size: 971 1631 3828
Fragments arranged by size:
3828 1631 971
BbvCI CC'TCA_GC
Cuts at: 2540 2540
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2156 2190 4621 4655 1903
Size: 34 50 34 135 34 2431 34 3678
Fragments arranged by size:
3678 2431 135 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BfrBI ATG'CAT
Cuts at: 2273 2273
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2289 2378 2451
Size: 107 122 71 631 605 617 89 73
Cuts at: 2451 2460 5041 136
Size: 9 2581 1525
Fragments arranged by size:
2581 1525 631 617 605 122 107 89
73 71 9
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 2178 3722 3847 2178
Size: 1544 125 4761
Fragments arranged by size:
4761 1544 125
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2390 2408 4491 5647 1790
Size: 262 338 18 2083 1156 2573
Fragments arranged by size:
2573 2083 1156 338 262 18
BmgBI CAC'GTC
Cuts at: 1318 3454 3700 1318
Size: 2136 246 4048
Fragments arranged by size:
4048 2136 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2385 3275
Size: 416 53 83 399 24 682 286 890
Cuts at: 3275 4161 5119 442
Size: 886 958 1753
Fragments arranged by size:
1753 958 890 886 682 416 399 286
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2834 3074 3515 2489
Size: 345 240 441 5404
Fragments arranged by size:
5404 441 345 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 2385 2430 2968 3035 3069 3449 3539
Size: 1131 15 45 538 67 34 380 90
Cuts at: 3539 5090 1239
Size: 1551 2579
Fragments arranged by size:
2579 1551 1131 538 380 90 67 45
34 15
Bpu10I CC'TnA_GC
Cuts at: 2540 2633 2540
Size: 93 6337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 4555 5420 5718 2234
Size: 2321 865 298 2946
Fragments arranged by size:
2946 2321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2153 2477 3415 5093 882
Size: 617 654 324 938 1678 2219
Fragments arranged by size:
2219 1678 938 654 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2862 3648 4237 4619
Size: 53 83 186 936 1329 786 589 382
Cuts at: 4619 5254 275
Size: 635 1451
Fragments arranged by size:
1451 1329 936 786 635 589 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2493 2523 2966 2996 1178
Size: 30 165 30 1090 30 443 30 4612
Fragments arranged by size:
4612 1090 443 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2514 3066 1186
Size: 1328 552 4550
Fragments arranged by size:
4550 1328 552
BseYI C'CCAG_C
Cuts at: 2182 2401 2902 2923 3141 3414 3637 5633 6129
Size: 219 501 21 218 273 223 1996 496
Cuts at: 6129 2182
Size: 2483
Fragments arranged by size:
2483 1996 501 496 273 223 219 218
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5857
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 4491 4576 5647 2523
Size: 1968 85 1071 3306
Fragments arranged by size:
3306 1968 1071 85
BsmI GAATG_Cn'
Cuts at: 2275 3611 3941 4034 2275
Size: 1336 330 93 4671
Fragments arranged by size:
4671 1336 330 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2390 2408 2523 3173 3274 3749
Size: 399 262 338 18 115 650 101 475
Cuts at: 3749 4491 4576 5647 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 475 399 338 262
115 101 85 18
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 3501 4319 6049 3501
Size: 818 1730 3882
Fragments arranged by size:
3882 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3785 844
Size: 2941 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3630 3691 4925 5099 65
Size: 1183 2382 61 1234 174 1396
Fragments arranged by size:
2382 1396 1234 1183 174 61
BsrFI r'CCGG_y
Cuts at: 1700 1810 2331 5074 5261 1700
Size: 110 521 2743 187 2869
Fragments arranged by size:
2869 2743 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 2640 96
Size: 2544 3886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 2828 2930 4490 5502 2828
Size: 102 1560 1012 3756
Fragments arranged by size:
3756 1560 1012 102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 3078 3166 3427 2830
Size: 248 88 261 5833
Fragments arranged by size:
5833 261 248 88
BstYI r'GATC_y
Cuts at: 1972 2208 3327 3760 4127 4512 4529 5981 6067
Size: 236 1119 433 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1119 433 385 367 346 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2730 3120 513
Size: 552 1665 390 3823
Fragments arranged by size:
3823 1665 552 390
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2674 2782 487
Size: 683 56 1448 108 4135
Fragments arranged by size:
4135 1448 683 108 56
BtsI GCAGTG_nn'
Cuts at: 2172 2266 3393 3946 4740 4760 2172
Size: 94 1127 553 794 20 3842
Fragments arranged by size:
3842 1127 794 553 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2087 5437 809
Size: 507 771 3350 1802
Fragments arranged by size:
3350 1802 771 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 2856 3080
Size: 54 1425 50 275 18 822 204 224
Cuts at: 3080 3147 3390 3877 4766 6395 8
Size: 67 243 487 889 1629 43
Fragments arranged by size:
1629 1425 889 822 487 275 243 224
204 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 3231 4360 1353
Size: 1307 571 1129 3423
Fragments arranged by size:
3423 1307 1129 571
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2478 5017 5391 5537 387
Size: 1064 138 889 2539 374 146 1280
Fragments arranged by size:
2539 1280 1064 889 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2385 2430 2968 3035 3069 3449
Size: 393 738 15 45 538 67 34 380
Cuts at: 3449 3539 4493 5090 5877 6145 1239
Size: 90 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 738 597 538 393 380
268 90 67 45 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 3578 2042
Size: 1536 4894
EcoO109I rG'GnC_Cy
Cuts at: 2096 2236 2290 3709 2096
Size: 140 54 1419 4817
Fragments arranged by size:
4817 1419 140 54
EcoRI G'AATT_C
Cuts at: 3008 3464 3739 4117 3008
Size: 456 275 378 5321
Fragments arranged by size:
5321 456 378 275
EcoRV GAT'ATC
Cuts at: 3217 3217
Size: 6430
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 3377 5577 1678
Size: 1699 2200 2531
Fragments arranged by size:
2531 2200 1699
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2493 2525 2575 2607 3045
Size: 32 135 32 921 32 50 32 438
Cuts at: 3045 3077 3261 3293 5071 5103 5145 5177 1373
Size: 32 184 32 1778 32 42 32 2626
Fragments arranged by size:
2626 1778 921 438 184 135 50 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3684 5544 5728 1305
Size: 2379 1860 184 2007
Fragments arranged by size:
2379 2007 1860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 3082 3149 3392 10
Size: 54 1425 343 822 428 67 243 3048
Fragments arranged by size:
3048 1425 822 428 343 243 67 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 3076 3452 3581 3698 4388 4747
Size: 648 1482 428 376 129 117 690 359
Cuts at: 4747 4906 518
Size: 159 2042
Fragments arranged by size:
2042 1482 690 648 428 376 359 159
129 117
MspA1I CmG'CkG
Cuts at: 1606 2182 2426 2923 3086 3145 3846 4523 5671
Size: 576 244 497 163 59 701 677 1148
Cuts at: 5671 5916 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 701 677 576 497 245 244
163 59
NcoI C'CATG_G
Cuts at: 513 1065 2730 3120 513
Size: 552 1665 390 3823
Fragments arranged by size:
3823 1665 552 390
NdeI CA'TA_TG
Cuts at: 387 2275 387
Size: 1888 4542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NsiI A_TGCA'T
Cuts at: 2275 2275
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2419 3203 3232 3788 5333 1132
Size: 97 1190 784 29 556 1545 2229
Fragments arranged by size:
2229 1545 1190 784 556 97 29
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6430
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PfoI T'CCnGG_A
Cuts at: 2682 3012 2682
Size: 330 6100
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2236 3709 2096
Size: 140 1473 4817
Fragments arranged by size:
4817 1473 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2386 2386
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2013 2346 3192 3363 3782 830
Size: 1183 333 846 171 419 3478
Fragments arranged by size:
3478 1183 846 419 333 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2182 2426 2923 3086 3145 2182
Size: 244 497 163 59 5467
Fragments arranged by size:
5467 497 244 163 59
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SapI GCTCTTCn'nnn_
Cuts at: 3231 3231
Size: 6430
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2009 2342 3188 3359 3778 4913 5594
Size: 219 964 333 846 171 419 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
1471 1135 964 846 681 419 333 219
191 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 2289 2451 2289
Size: 162 6268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 2331 1700
Size: 631 5799
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 4534 5435 5697 820
Size: 197 926 270 2321 901 262 1553
Fragments arranged by size:
2321 1553 926 901 270 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 2419 3203 3788 1132
Size: 1287 784 585 3774
Fragments arranged by size:
3774 1287 784 585
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2052 2446 2476 2715 2730 2782 3120
Size: 552 987 394 30 239 15 52 338
Cuts at: 3120 3282 3869 513
Size: 162 587 3074
Fragments arranged by size:
3074 987 587 552 394 338 239 162
52 30 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2612 3113 4457 4474 4627 4812
Size: 1255 465 567 501 1344 17 153 185
Cuts at: 4812 5257 325
Size: 445 1498
Fragments arranged by size:
1498 1344 1255 567 501 465 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 2973 4676
Size: 274 80 33 51 1443 663 333 1703
Cuts at: 4676 6388 96
Size: 1712 138
Fragments arranged by size:
1712 1703 1443 663 333 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 6390 6422 2731
Size: 32 3627 32 2739
Fragments arranged by size:
3627 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3205 3766 3205
Size: 561 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2893 3142 3262 2380
Size: 513 249 120 5548
Fragments arranged by size:
5548 513 249 120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 3240 4559 3240
Size: 1319 5111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2863 4238 276
Size: 53 83 186 936 1329 1375 2468
Fragments arranged by size:
2468 1375 1329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfoI SrfI SwaI