Restriction map for
pFN21AB7201
(Circular) (Six-base) MAPSORT of: pFN21AB7201.seq Check: 2463 from: 1 to: 6787
pFN21AB7201 6787 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4011 4597 278
Size: 53 83 186 936 2475 586 2468
Fragments arranged by size:
2475 2468 936 586 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3213 4130 1937
Size: 1276 917 4594
Fragments arranged by size:
4594 1276 917
Acc65I G'GTAC_C
Cuts at: 4108 4108
Size: 6787
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4850 6234 6502 1632
Size: 3218 1384 268 1917
Fragments arranged by size:
3218 1917 1384 268
AfeI AGC'GCT
Cuts at: 2066 2918 2066
Size: 852 5935
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6590
AflIII A'CryG_T
Cuts at: 1203 5686 6663 1203
Size: 4483 977 1327
Fragments arranged by size:
4483 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6787
AlwNI CAG_nnn'CTG
Cuts at: 3436 4530 6102 3436
Size: 1094 1572 4121
Fragments arranged by size:
4121 1572 1094
ApaLI G'TGCA_C
Cuts at: 2210 3190 3853 4844 6000 2210
Size: 980 663 991 1156 2997
Fragments arranged by size:
2997 1156 991 980 663
ApoI r'AATT_y
Cuts at: 1493 4096 4322 4474 6583 1493
Size: 2603 226 152 2109 1697
Fragments arranged by size:
2603 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3033 3033
Size: 6787
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2420 2693 3112 3802 4112 1870
Size: 73 477 273 419 690 310 4545
Fragments arranged by size:
4545 690 477 419 310 273 73
BamHI G'GATC_C
Cuts at: 4117 4484 4117
Size: 367 6420
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2620 2697 3463 3491 3881
Size: 325 217 627 833 77 766 28 390
Cuts at: 3881 3905 4108 5563 618
Size: 24 203 1455 1842
Fragments arranged by size:
1842 1455 833 766 627 390 325 217
203 77 28 24
BanII G_rGCy'C
Cuts at: 1391 2694 3995 4049 4106 1391
Size: 1303 1301 54 57 4072
Fragments arranged by size:
4072 1303 1301 57 54
BbeI G_GCGC'C
Cuts at: 2624 3467 3885 3909 2624
Size: 843 418 24 5502
Fragments arranged by size:
5502 843 418 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2830 3193 3751 928
Size: 971 931 363 558 3964
Fragments arranged by size:
3964 971 931 558 363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4978 5012 1903
Size: 34 3041 34 3678
Fragments arranged by size:
3678 3041 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2090 2379
Size: 107 122 71 631 605 379 39 289
Cuts at: 2379 5398 136
Size: 3019 1525
Fragments arranged by size:
3019 1525 631 605 379 289 122 107
71 39
BglII A'GATC_T
Cuts at: 6782 6782
Size: 6787
BlpI GC'TnA_GC
Cuts at: 3380 4204 3380
Size: 824 5963
Bme1580I G_kGCm'C
Cuts at: 1790 2214 3194 3351 3633 3857 4040 4848 6004
Size: 424 980 157 282 224 183 808 1156
Cuts at: 6004 1790
Size: 2573
Fragments arranged by size:
2573 1156 980 808 424 282 224 183
157
BmgBI CAC'GTC
Cuts at: 1318 4026 1318
Size: 2708 4079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4518 5476 442
Size: 416 53 83 399 24 3101 958 1753
Fragments arranged by size:
3101 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 3602 3854 5447 1239
Size: 986 1377 252 1593 2579
Fragments arranged by size:
2579 1593 1377 986 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 3752 4912 5777 6075 2240
Size: 1512 1160 865 298 2952
Fragments arranged by size:
2952 1512 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 5450 882
Size: 617 1665 2286 2219
Fragments arranged by size:
2286 2219 1665 617
BsaAI yAC'GTr
Cuts at: 493 1204 3160 3421 4578 493
Size: 711 1956 261 1157 2702
Fragments arranged by size:
2702 1956 1157 711 261
BsaBI GATnn'nnATC
Cuts at: 6781 6781
Size: 6787
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2621 3464 3882 3906
Size: 53 83 186 936 1088 843 418 24
Cuts at: 3906 4008 4594 4976 5611 275
Size: 102 586 382 635 1451
Fragments arranged by size:
1451 1088 936 843 635 586 418 382
186 102 83 53 24
BsaWI w'CCGG_w
Cuts at: 5220 5618 5892 6039 6450 5220
Size: 398 274 147 411 5557
Fragments arranged by size:
5557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 1178
Size: 30 165 30 822 30 5710
Fragments arranged by size:
5710 822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3149 1186
Size: 1963 4824
BseYI C'CCAG_C
Cuts at: 2486 2626 3053 3139 3350 3684 3867 5990 6486
Size: 140 427 86 211 334 183 2123 496
Cuts at: 6486 2486
Size: 2787
Fragments arranged by size:
2787 2123 496 427 334 211 183 140
86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 3043 1163
Size: 573 554 753 4907
Fragments arranged by size:
4907 753 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 2398 2413 2554 3279 3915
Size: 275 186 84 313 15 141 725 636
Cuts at: 3915 4034 4237 4998 6026 1540
Size: 119 203 761 1028 2301
Fragments arranged by size:
2301 1028 761 725 636 313 275 203
186 141 119 84 15
BsiHKAI G_wGCw'C
Cuts at: 2214 2246 2865 3194 3621 3857 4049 4848 4933
Size: 32 619 329 427 236 192 799 85
Cuts at: 4933 6004 2214
Size: 1071 2997
Fragments arranged by size:
2997 1071 799 619 427 329 236 192
85 32
BsmI GAATG_Cn'
Cuts at: 4298 4391 4298
Size: 93 6694
BsmBI CGTCTCn'nnnn_
Cuts at: 2896 2896
Size: 6787
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2246 2694 2865 3194 3351 3621
Size: 399 424 32 448 171 329 157 270
Cuts at: 3621 3633 3857 3995 4040 4049 4106 4848 4933
Size: 12 224 138 45 9 57 742 85
Cuts at: 4933 6004 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 448 424 399 329 270
224 171 157 138 85 57 45 32
12 9
BspEI T'CCGG_A
Cuts at: 6450 6450
Size: 6787
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3453 3729 4142 844
Size: 2609 276 413 3489
Fragments arranged by size:
3489 2609 413 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 2365 2746 2935 3537 4674 1394
Size: 429 505 37 381 189 602 1137 3507
Fragments arranged by size:
3507 1137 602 505 429 381 189 37
BsrDI GCAATG_nn'
Cuts at: 65 1248 5282 5456 65
Size: 1183 4034 174 1396
Fragments arranged by size:
4034 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2351 2412 3176 3278 3308 3509 5431
Size: 110 541 61 764 102 30 201 1922
Cuts at: 5431 5618 1700
Size: 187 2869
Fragments arranged by size:
2869 1922 764 541 201 187 110 102
61 30
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 4606
BssHII G'CGCG_C
Cuts at: 1925 2341 2358 2709 2784 2824 3033 1925
Size: 416 17 351 75 40 209 5679
Fragments arranged by size:
5679 416 351 209 75 40 17
BssSI C'ACGA_G
Cuts at: 3008 4847 5859 3008
Size: 1839 1012 3936
Fragments arranged by size:
3936 1839 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2406 3160 3861 794
Size: 1612 754 701 3720
Fragments arranged by size:
3720 1612 754 701
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 2015 1184
Size: 831 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2101
Size: 6787
BstYI r'GATC_y
Cuts at: 1972 2235 4117 4484 4869 4886 6338 6424 6436
Size: 263 1882 367 385 17 1452 86 12
Cuts at: 6436 6782 1972
Size: 346 1977
Fragments arranged by size:
1977 1882 1452 385 367 346 263 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2548 2581 3195 3636 3885 3909
Size: 552 939 544 33 614 441 249 24
Cuts at: 3909 3915 513
Size: 6 3385
Fragments arranged by size:
3385 939 614 552 544 441 249 33
24 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3289 3349 487
Size: 683 56 2063 60 3925
Fragments arranged by size:
3925 2063 683 60 56
BtsI GCAGTG_nn'
Cuts at: 4303 5097 5117 4303
Size: 794 20 5973
Fragments arranged by size:
5973 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6787
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DraIII CAC_nnn'GTG
Cuts at: 2445 2445
Size: 6787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3755 3835 4030 5794 809
Size: 507 2439 80 195 1764 1802
Fragments arranged by size:
2439 1802 1764 507 195 80
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2082 2091
Size: 54 1425 50 275 18 213 39 9
Cuts at: 2091 2222 2545 2551 3228 3276 3333 3507 3912
Size: 131 323 6 677 48 57 174 405
Cuts at: 3912 4234 5123 6752 8
Size: 322 889 1629 43
Fragments arranged by size:
1629 1425 889 677 405 323 322 275
213 174 131 57 54 50 48 43
39 18 9 6
EagI C'GGCC_G
Cuts at: 1537 1812 2082 2551 3276 3912 4234 1537
Size: 275 270 469 725 636 322 4090
Fragments arranged by size:
4090 725 636 469 322 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 2972 3488 3530 3662 4717 1353
Size: 1619 516 42 132 1055 3423
Fragments arranged by size:
3423 1619 1055 516 132 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3587 5374 5748 5894 387
Size: 1064 138 1998 1787 374 146 1280
Fragments arranged by size:
1998 1787 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4047 4047
Size: 6787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 3602 3854 4850 5447 6234 6502
Size: 393 593 1377 252 996 597 787 268
Cuts at: 6502 1239
Size: 1524
Fragments arranged by size:
1524 1377 996 787 597 593 393 268
252
EcoO109I rG'GnC_Cy
Cuts at: 2571 3718 3956 2571
Size: 1147 238 5402
Fragments arranged by size:
5402 1147 238
EcoRI G'AATT_C
Cuts at: 4096 4474 4096
Size: 378 6409
FspI TGC'GCA
Cuts at: 5293 5293
Size: 6787
HaeII r_GCGC'y
Cuts at: 1678 2057 2068 2079 2624 2740 2920 3054 3380
Size: 379 11 11 545 116 180 134 326
Cuts at: 3380 3467 3847 3885 3909 5934 1678
Size: 87 380 38 24 2025 2531
Fragments arranged by size:
2531 2025 545 380 379 326 180 134
116 87 38 24 11 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5428 5460 5502 5534 1373
Size: 32 135 32 3856 32 42 32 2626
Fragments arranged by size:
3856 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4131 4377 669
Size: 988 281 2193 246 3079
Fragments arranged by size:
3079 2193 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6787
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KasI G'GCGC_C
Cuts at: 2620 3463 3881 3905 2620
Size: 843 418 24 5502
Fragments arranged by size:
5502 843 418 24
KpnI G_GTAC'C
Cuts at: 4112 4112
Size: 6787
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 5901 6085 1305
Size: 699 3897 184 2007
Fragments arranged by size:
3897 2007 699 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3230 10
Size: 54 1425 343 1398 3567
Fragments arranged by size:
3567 1425 1398 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3368 4745 5104 5263 518
Size: 648 993 1209 1377 359 159 2042
Fragments arranged by size:
2042 1377 1209 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2227 2314 2544 2583 3027 3469 3638
Size: 522 99 87 230 39 444 442 169
Cuts at: 3638 3861 3887 3917 4203 4880 6028 6273 1606
Size: 223 26 30 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 522 444 442 286 245
230 223 169 99 87 39 30 26
NaeI GCC'GGC
Cuts at: 2353 3310 3511 2353
Size: 957 201 5629
Fragments arranged by size:
5629 957 201
NarI GG'CG_CC
Cuts at: 2621 3464 3882 3906 2621
Size: 843 418 24 5502
Fragments arranged by size:
5502 843 418 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5296
Fragments arranged by size:
5296 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6787
NgoMIV G'CCGG_C
Cuts at: 2351 3308 3509 2351
Size: 957 201 5629
Fragments arranged by size:
5629 957 201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 2082 4234 2082
Size: 2152 4635
NspI r_CATG'y
Cuts at: 1132 1229 2109 2164 2278 2404 4145 5690 1132
Size: 97 880 55 114 126 1741 1545 2229
Fragments arranged by size:
2229 1741 1545 880 126 114 97 55
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6787
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3243 3436 1108
Size: 902 1233 193 4459
Fragments arranged by size:
4459 1233 902 193
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PmlI CAC'GTG
Cuts at: 3160 3160
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3718 3956 3718
Size: 238 6549
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6787
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PstI C_TGCA'G
Cuts at: 830 2064 3726 4139 830
Size: 1234 1662 413 3478
Fragments arranged by size:
3478 1662 1234 413
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
SacI G_AGCT'C
Cuts at: 4049 4049
Size: 6787
SacII CC_GC'GG
Cuts at: 2584 3639 3888 3918 2584
Size: 1055 249 30 5453
Fragments arranged by size:
5453 1055 249 30
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SanDI GG'GwC_CC
Cuts at: 3956 3956
Size: 6787
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 3323 3773 5035 6747 3323
Size: 450 1262 1712 3363
Fragments arranged by size:
3363 1712 1262 450
SfcI C'TryA_G
Cuts at: 826 1045 2060 3722 4000 4135 5270 5951 6142
Size: 219 1015 1662 278 135 1135 681 191
Cuts at: 6142 826
Size: 1471
Fragments arranged by size:
1662 1471 1135 1015 681 278 219 191
135
SfiI GGCCn_nnn'nGGCC
Cuts at: 2090 2090
Size: 6787
SfoI GGC'GCC
Cuts at: 2622 3465 3883 3907 2622
Size: 843 418 24 5502
Fragments arranged by size:
5502 843 418 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6787
SmaI CCC'GGG
Cuts at: 1872 2695 3114 4114 1872
Size: 823 419 1000 4545
Fragments arranged by size:
4545 1000 823 419
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 3767 4891 5792 6054 820
Size: 197 926 312 1512 1124 901 262 1553
Fragments arranged by size:
1553 1512 1124 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 2109 2404 4145 1132
Size: 977 295 1741 3774
Fragments arranged by size:
3774 1741 977 295
SrfI GCCC'GGGC
Cuts at: 3114 3114
Size: 6787
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6787
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2094 2346 4226 513
Size: 552 939 45 45 252 1880 3074
Fragments arranged by size:
3074 1880 939 552 252 45 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3235 3529 4814 4831 4984 5169 5614
Size: 1255 1655 294 1285 17 153 185 445
Cuts at: 5614 325
Size: 1498
Fragments arranged by size:
1655 1498 1285 1255 445 294 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 3321 3771
Size: 274 80 33 51 1443 300 1044 450
Cuts at: 3771 5033 6745 96
Size: 1262 1712 138
Fragments arranged by size:
1712 1443 1262 1044 450 300 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 6747 6779 2225
Size: 32 4490 32 2233
Fragments arranged by size:
4490 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6787
XbaI T'CTAG_A
Cuts at: 4123 4123
Size: 6787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3370 3502 3370
Size: 132 6655
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6787
XmaI C'CCGG_G
Cuts at: 1870 2693 3112 4112 1870
Size: 823 419 1000 4545
Fragments arranged by size:
4545 1000 823 419
XmnI GAAnn'nnTTC
Cuts at: 4916 4916
Size: 6787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4009 4595 276
Size: 53 83 186 936 2475 586 2468
Fragments arranged by size:
2475 2468 936 586 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI
PvuII RsrII SapI SexAI SwaI