Restriction map for
pFN21AB7200
(Circular) (Six-base) MAPSORT of: pFN21AB7200.seq Check: 5160 from: 1 to: 5998
pFN21AB7200 5998 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 3261 2350
Size: 911 5087
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3109 4061 5445 5713 1632
Size: 1477 952 1384 268 1917
Fragments arranged by size:
1917 1477 1384 952 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2524 1184
Size: 1340 4658
AlwNI CAG_nnn'CTG
Cuts at: 2728 2740 3741 5313 2728
Size: 12 1001 1572 3413
Fragments arranged by size:
3413 1572 1001 12
ApaI G_GGCC'C
Cuts at: 2119 2126 2167 2267 2119
Size: 7 41 100 5850
Fragments arranged by size:
5850 100 41 7
ApaLI G'TGCA_C
Cuts at: 2363 2529 4055 5211 2363
Size: 166 1526 1156 3150
Fragments arranged by size:
3150 1526 1156 166
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2898 3323 1870
Size: 73 175 780 425 4545
Fragments arranged by size:
4545 780 425 175 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2934 3319 4774 618
Size: 325 217 627 455 692 385 1455 1842
Fragments arranged by size:
1842 1455 692 627 455 385 325 217
BanII G_rGCy'C
Cuts at: 1391 2119 2126 2167 2267 2381 2573 2840 3317
Size: 728 7 41 100 114 192 267 477
Cuts at: 3317 1391
Size: 4072
Fragments arranged by size:
4072 728 477 267 192 114 100 41
7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2416 2617 928
Size: 971 517 201 4309
Fragments arranged by size:
4309 971 517 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4189 4223 1903
Size: 34 50 34 2168 34 3678
Fragments arranged by size:
3678 2168 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 2388 1548
Size: 840 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2774 2783 4609
Size: 107 122 71 631 605 1102 9 1826
Cuts at: 4609 136
Size: 1525
Fragments arranged by size:
1826 1525 1102 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2149 3090 5993 2149
Size: 941 2903 2154
Fragments arranged by size:
2903 2154 941
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2126 2167 2257 2267 2367 2533 2565
Size: 329 7 41 90 10 100 166 32
Cuts at: 2565 2695 4059 5215 1790
Size: 130 1364 1156 2573
Fragments arranged by size:
2573 1364 1156 329 166 130 100 90
41 32 10 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2427 2520 3729
Size: 416 53 83 399 24 1010 93 1209
Cuts at: 3729 4687 442
Size: 958 1753
Fragments arranged by size:
1753 1209 1010 958 416 399 93 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2276 2393 2690 2750 2768 3048 3077
Size: 862 175 117 297 60 18 280 29
Cuts at: 3077 3257 4658 1239
Size: 180 1401 2579
Fragments arranged by size:
2579 1401 862 297 280 180 175 117
60 29 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2633 4123 4988 5286 2417
Size: 216 1490 865 298 3129
Fragments arranged by size:
3129 1490 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2928 4431 4829 5103 5250 5661 2928
Size: 1503 398 274 147 411 3265
Fragments arranged by size:
3265 1503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 2685 2715 2822
Size: 30 165 30 868 30 384 30 107
Cuts at: 2822 2852 1178
Size: 30 4324
Fragments arranged by size:
4324 868 384 165 107 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2279 2693 1186
Size: 978 115 414 4491
Fragments arranged by size:
4491 978 414 115
BseYI C'CCAG_C
Cuts at: 2075 2110 2260 2380 2786 2881 3045 3266 5201
Size: 35 150 120 406 95 164 221 1935
Cuts at: 5201 5697 2075
Size: 496 2376
Fragments arranged by size:
2376 1935 496 406 221 164 150 120
95 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 2494 3161 1163
Size: 573 337 421 667 4000
Fragments arranged by size:
4000 667 573 421 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 2533 2573 4059 4144 5215 2367
Size: 166 40 1486 85 1071 3150
Fragments arranged by size:
3150 1486 1071 166 85 40
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2126 2167 2257 2267 2367 2381
Size: 399 329 7 41 90 10 100 14
Cuts at: 2381 2533 2565 2573 2695 2840 3317 4059 4144
Size: 152 32 8 122 145 477 742 85
Cuts at: 4144 5215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 477 399 329 152 145
122 100 90 85 41 32 14 10
8 7
BspEI T'CCGG_A
Cuts at: 2928 5661 2928
Size: 2733 3265
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2350 3261 3353 844
Size: 1271 235 911 92 3489
Fragments arranged by size:
3489 1271 911 235 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2497 4642 4829 1700
Size: 110 687 2145 187 2869
Fragments arranged by size:
2869 2145 687 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2743 794
Size: 1949 4049
BstBI TT'CG_AA
Cuts at: 2628 4802 2628
Size: 2174 3824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2786 2786
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2149 3090 3328 3695 4080 4097 5549 5635
Size: 177 941 238 367 385 17 1452 86
Cuts at: 5635 5647 5993 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 941 385 367 346 238 177
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2950 3225 513
Size: 552 1885 275 3286
Fragments arranged by size:
3286 1885 552 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2187 487
Size: 683 56 961 4298
Fragments arranged by size:
4298 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 2501 2579 2653 3514 4308 4328 2222
Size: 279 78 74 861 794 20 3892
Fragments arranged by size:
3892 861 794 279 78 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 3445 4334
Size: 54 1425 50 275 18 639 976 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 976 889 639 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2625 3928 1353
Size: 829 443 1303 3423
Fragments arranged by size:
3423 1303 829 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2678 3149 4585 4959 5105 387
Size: 1064 138 1089 471 1436 374 146 1280
Fragments arranged by size:
1436 1280 1089 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 2571 2571
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2276 2393 2690 2750 2768 3048
Size: 393 469 175 117 297 60 18 280
Cuts at: 3048 3077 3109 3257 4061 4658 5445 5713 1239
Size: 29 32 148 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 469 393 297 280
268 175 148 117 60 32 29 18
EcoO109I rG'GnC_Cy
Cuts at: 2115 2122 2163 2264 2870 2905 2115
Size: 7 41 101 606 35 5208
Fragments arranged by size:
5208 606 101 41 35 7
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 4639 4671 4713
Size: 32 135 32 1250 32 1785 32 42
Cuts at: 4713 4745 1373
Size: 32 2626
Fragments arranged by size:
2626 1785 1250 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 3303 5112 5296 1305
Size: 1548 450 1809 184 2007
Fragments arranged by size:
2007 1809 1548 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 10
Size: 54 1425 343 639 3537
Fragments arranged by size:
3537 1425 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3110 3956 4315 4474 518
Size: 648 1944 846 359 159 2042
Fragments arranged by size:
2042 1944 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2209 2215 2740 3045 3076 3414 4091
Size: 473 130 6 525 305 31 338 677
Cuts at: 4091 5239 5484 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 473 338 305 245
130 31 6
NaeI GCC'GGC
Cuts at: 2499 2499
Size: 5998
NcoI C'CATG_G
Cuts at: 513 1065 2950 513
Size: 552 1885 3561
Fragments arranged by size:
3561 1885 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2497 2497
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3013 3115 3356 4901 1132
Size: 97 1784 102 241 1545 2229
Fragments arranged by size:
2229 1784 1545 241 102 97
PasI CC'CwG_GG
Cuts at: 1348 2126 1348
Size: 778 5220
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 2986 1108
Size: 1566 312 4120
Fragments arranged by size:
4120 1566 312
PfoI T'CCnGG_A
Cuts at: 2292 3054 2292
Size: 762 5236
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4067 4099 5580 5612 3224
Size: 32 811 32 1481 32 3610
Fragments arranged by size:
3610 1481 811 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspOMI G'GGCC_C
Cuts at: 2115 2122 2163 2263 2115
Size: 7 41 100 5850
Fragments arranged by size:
5850 100 41 7
PstI C_TGCA'G
Cuts at: 830 2214 2361 3350 830
Size: 1384 147 989 3478
Fragments arranged by size:
3478 1384 989 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2079 2209 2215 2740 2079
Size: 130 6 525 5337
Fragments arranged by size:
5337 525 130 6
SacI G_AGCT'C
Cuts at: 2573 2573
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 5998
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2210 2357 3346 4481 5162 5353 826
Size: 219 1165 147 989 1135 681 191 1471
Fragments arranged by size:
1471 1165 1135 989 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 2120 2900 3325 1872
Size: 248 780 425 4545
Fragments arranged by size:
4545 780 425 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 2648 4102 5003 5265 820
Size: 197 926 489 216 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 489 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 2020 1517
Size: 503 5495
StyI C'CwwG_G
Cuts at: 513 1065 2043 2050 2805 2866 2950 3437 513
Size: 552 978 7 755 61 84 487 3074
Fragments arranged by size:
3074 978 755 552 487 84 61 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2107 2419 2473 2548 4025 4042 4195
Size: 1255 527 312 54 75 1477 17 153
Cuts at: 4195 4380 4825 325
Size: 185 445 1498
Fragments arranged by size:
1498 1477 1255 527 445 312 185 153
75 54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 5956 96
Size: 274 80 33 51 1443 2267 1712 138
Fragments arranged by size:
2267 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2685 2717 5958 5990 2685
Size: 32 3241 32 2693
Fragments arranged by size:
3241 2693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 2118 2898 3323 1870
Size: 248 780 425 4545
Fragments arranged by size:
4545 780 425 248
XmnI GAAnn'nnTTC
Cuts at: 2624 4127 2624
Size: 1503 4495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI