Restriction map for
pFN21AB7199
(Circular) (Six-base) MAPSORT of: pFN21AB7199.seq Check: 7396 from: 1 to: 6106
pFN21AB7199 6106 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 2803 4169 5553 5821 1632
Size: 558 613 1366 1384 268 1917
Fragments arranged by size:
1917 1384 1366 613 558 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 2845 5005 5982 1203
Size: 1642 2160 977 1327
Fragments arranged by size:
2160 1642 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 3063 1166
Size: 1897 4209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 2755 3849 5421 2755
Size: 1094 1572 3440
Fragments arranged by size:
3440 1572 1094
ApaI G_GGCC'C
Cuts at: 2712 2873 2712
Size: 161 5945
ApaLI G'TGCA_C
Cuts at: 2966 4163 5319 2966
Size: 1197 1156 3753
Fragments arranged by size:
3753 1197 1156
ApoI r'AATT_y
Cuts at: 1493 3086 3415 3641 3793 5902 1493
Size: 1593 329 226 152 2109 1697
Fragments arranged by size:
2109 1697 1593 329 226 152
AscI GG'CGCG_CC
Cuts at: 2064 2064
Size: 6106
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2398 2465 2492 2913 3001 3202 3431
Size: 73 455 67 27 421 88 201 229
Cuts at: 3431 1870
Size: 4545
Fragments arranged by size:
4545 455 421 229 201 88 73 67
27
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2517 2714 3427 4882
Size: 325 217 627 518 212 197 713 1455
Cuts at: 4882 618
Size: 1842
Fragments arranged by size:
1842 1455 713 627 518 325 217 212
197
BanII G_rGCy'C
Cuts at: 1391 2183 2219 2385 2421 2472 2712 2873 3209
Size: 792 36 166 36 51 240 161 336
Cuts at: 3209 3425 1391
Size: 216 4072
Fragments arranged by size:
4072 792 336 240 216 166 161 51
36 36
BbeI G_GCGC'C
Cuts at: 2718 2718
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2425 2459 2752 2786 3193 3227 4297
Size: 34 488 34 293 34 407 34 1070
Cuts at: 4297 4331 1903
Size: 34 3678
Fragments arranged by size:
3678 1070 488 407 293 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BfrBI ATG'CAT
Cuts at: 2830 3142 2830
Size: 312 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 2533 4717
Size: 107 122 71 631 605 760 101 2184
Cuts at: 4717 136
Size: 1525
Fragments arranged by size:
2184 1525 760 631 605 122 107 101
71
BglII A'GATC_T
Cuts at: 3015 6101 3015
Size: 3086 3020
BlpI GC'TnA_GC
Cuts at: 2177 2781 2786 3523 2177
Size: 604 5 737 4760
Fragments arranged by size:
4760 737 604 5
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2520 2538 2712 2873 2912 2970 4167
Size: 520 210 18 174 161 39 58 1197
Cuts at: 4167 5323 1790
Size: 1156 2573
Fragments arranged by size:
2573 1197 1156 520 210 174 161 58
39 18
BmgBI CAC'GTC
Cuts at: 1318 2890 1318
Size: 1572 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2600 2908 3837
Size: 416 53 83 399 24 1183 308 929
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1183 958 929 416 399 308 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 4766 1239
Size: 1197 2330 2579
Fragments arranged by size:
2579 2330 1197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3225 4231 5096 5394 3023
Size: 202 1006 865 298 3735
Fragments arranged by size:
3735 1006 865 298 202
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2539 3897 493
Size: 711 1335 1358 2702
Fragments arranged by size:
2702 1358 1335 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2715 3913 4295 4930
Size: 53 83 186 936 1182 1198 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1451 1198 1182 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2811 4539 4937 5211 5358 5769 2811
Size: 1728 398 274 147 411 3148
Fragments arranged by size:
3148 1728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2676 2706 2744 2774 2909
Size: 30 165 30 1273 30 38 30 135
Cuts at: 2909 2939 3191 3221 1178
Size: 30 252 30 4063
Fragments arranged by size:
4063 1273 252 165 135 38 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 1186
Size: 1732 4374
BseYI C'CCAG_C
Cuts at: 2044 2309 2513 5309 5805 2044
Size: 265 204 2796 496 2345
Fragments arranged by size:
2796 2345 496 265 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 2640 2812 3127 3556 4317
Size: 275 186 586 53 172 315 429 761
Cuts at: 4317 5345 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 586 429 315 275 186
172 53
BsiHKAI G_wGCw'C
Cuts at: 2219 2970 3209 4167 4252 5323 2219
Size: 751 239 958 85 1071 3002
Fragments arranged by size:
3002 1071 958 751 239 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2219 2310 2385 2421 2472 2520
Size: 399 393 36 91 75 36 51 48
Cuts at: 2520 2538 2712 2873 2912 2970 3209 3425 4167
Size: 18 174 161 39 58 239 216 742
Cuts at: 4167 4252 5323 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 239 216 174
161 91 85 75 58 51 48 39
36 36 18
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3232 3995 5725 3232
Size: 763 1730 3613
Fragments arranged by size:
3613 1730 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2426 2639 2790 4750 4937 1700
Size: 110 288 328 213 151 1960 187 2869
Fragments arranged by size:
2869 1960 328 288 213 187 151 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 2064 1925
Size: 139 5967
BssSI C'ACGA_G
Cuts at: 2078 4166 5178 2078
Size: 2088 1012 3006
Fragments arranged by size:
3006 2088 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3180 794
Size: 2386 3720
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 3371 2257
Size: 1114 4992
BstYI r'GATC_y
Cuts at: 1972 3015 3042 3436 3803 4188 4205 5657 5743
Size: 1043 27 394 367 385 17 1452 86
Cuts at: 5743 5755 6101 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1043 394 385 367 346 86
27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2187 2704 2853 513
Size: 552 1122 517 149 3766
Fragments arranged by size:
3766 1122 552 517 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4416 4436 3622
Size: 794 20 5292
Fragments arranged by size:
5292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2584 2610 2637
Size: 54 1425 50 275 18 754 26 27
Cuts at: 2637 3553 4442 6071 8
Size: 916 889 1629 43
Fragments arranged by size:
1629 1425 916 889 754 275 54 50
43 27 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2584 2637 3553 1537
Size: 275 772 53 916 4090
Fragments arranged by size:
4090 916 772 275 53
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 2795 4036 1353
Size: 1073 369 1241 3423
Fragments arranged by size:
3423 1241 1073 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 4693 5067 5213 387
Size: 1064 138 765 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 765 374 146 138
EcoICRI GAG'CTC
Cuts at: 2217 3207 2217
Size: 990 5116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2436 2803 4169 4766 5553 5821
Size: 393 558 246 367 1366 597 787 268
Cuts at: 5821 1239
Size: 1524
Fragments arranged by size:
1524 1366 787 597 558 393 367 268
246
EcoO109I rG'GnC_Cy
Cuts at: 2869 3054 2869
Size: 185 5921
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 3215 4612 3215
Size: 1397 4709
HaeII r_GCGC'y
Cuts at: 1678 2325 2514 2646 2718 5253 1678
Size: 647 189 132 72 2535 2531
Fragments arranged by size:
2535 2531 647 189 132 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 4747 4779 4821
Size: 32 135 32 1337 32 1806 32 42
Cuts at: 4821 4853 1373
Size: 32 2626
Fragments arranged by size:
2626 1806 1337 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2953 3450 3696 669
Size: 988 281 1015 497 246 3079
Fragments arranged by size:
3079 1015 988 497 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2714 2714
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 10
Size: 54 1425 343 780 3504
Fragments arranged by size:
3504 1425 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2672 2678 2684 2963 3063 3141
Size: 648 843 663 6 6 279 100 78
Cuts at: 3141 3143 4064 4423 4582 518
Size: 2 921 359 159 2042
Fragments arranged by size:
2042 921 843 663 648 359 279 159
100 78 6 6 2
MspA1I CmG'CkG
Cuts at: 1606 2165 2189 2456 2785 3522 4199 5347 5592
Size: 559 24 267 329 737 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 737 677 559 329 267 245
24
NaeI GCC'GGC
Cuts at: 2100 2428 2641 2792 2100
Size: 328 213 151 5414
Fragments arranged by size:
5414 328 213 151
NarI GG'CG_CC
Cuts at: 2715 2715
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 2853 513
Size: 552 1788 3766
Fragments arranged by size:
3766 1788 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 2098 2426 2639 2790 2098
Size: 328 213 151 5414
Fragments arranged by size:
5414 328 213 151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NsiI A_TGCA'T
Cuts at: 2832 3144 2832
Size: 312 5794
NspI r_CATG'y
Cuts at: 1132 1229 2450 2849 2864 3142 3146 3464 5009
Size: 97 1221 399 15 278 4 318 1545
Cuts at: 5009 1132
Size: 2229
Fragments arranged by size:
2229 1545 1221 399 318 278 97 15
4
PasI CC'CwG_GG
Cuts at: 1348 2039 2257 1348
Size: 691 218 5197
Fragments arranged by size:
5197 691 218
PciI A'CATG_T
Cuts at: 2845 5005 2845
Size: 2160 3946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PfoI T'CCnGG_A
Cuts at: 2016 2016
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PmlI CAC'GTG
Cuts at: 2539 2539
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2377 4175 4207 5688 5720 2345
Size: 32 1798 32 1481 32 2731
Fragments arranged by size:
2731 1798 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3054 3054
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 2846 1613
Size: 1233 4873
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 2708 2869 2708
Size: 161 5945
PstI C_TGCA'G
Cuts at: 830 3346 3458 830
Size: 2516 112 3478
Fragments arranged by size:
3478 2516 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2456 2785 2456
Size: 329 5777
SacI G_AGCT'C
Cuts at: 2219 3209 2219
Size: 990 5116
SacII CC_GC'GG
Cuts at: 2190 2190
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 2426 2795 2426
Size: 369 5737
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SexAI A'CCwGG_T
Cuts at: 2550 2550
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 2659 3300 3342 3454 4589 5270 5461
Size: 219 1614 641 42 112 1135 681 191
Cuts at: 5461 826
Size: 1471
Fragments arranged by size:
1614 1471 1135 681 641 219 191 112
42
SfoI GGC'GCC
Cuts at: 2716 2716
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 2494 2915 3003 3433 1872
Size: 622 421 88 430 4545
Fragments arranged by size:
4545 622 430 421 88
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 3240 4210 5111 5373 820
Size: 197 926 1095 202 970 901 262 1553
Fragments arranged by size:
1553 1095 970 926 901 262 202 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2450 3464 1132
Size: 1318 1014 3774
Fragments arranged by size:
3774 1318 1014
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2853 3545 513
Size: 552 1788 692 3074
Fragments arranged by size:
3074 1788 692 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2940 4352 6064
Size: 274 80 33 51 1443 963 1412 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1712 1443 1412 963 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 2744 2776 3191 3223 6066 6098 2676
Size: 32 36 32 415 32 2843 32 2684
Fragments arranged by size:
2843 2684 415 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 2833 2860 2536
Size: 297 27 5782
Fragments arranged by size:
5782 297 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 2492 2913 3001 3431 1870
Size: 622 421 88 430 4545
Fragments arranged by size:
4545 622 430 421 88
XmnI GAAnn'nnTTC
Cuts at: 2120 2984 3074 4235 2120
Size: 864 90 1161 3991
Fragments arranged by size:
3991 1161 864 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NruI PacI PspXI PsrI RsrII SanDI SfiI
SrfI SwaI