Restriction map for
pFN21AB7198
(Circular) (Six-base) MAPSORT of: pFN21AB7198.seq Check: 3914 from: 1 to: 6094
pFN21AB7198 6094 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2526 2526
Size: 6094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 2265 3415 2265
Size: 1150 4944
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 2494 2627 2785 3029 4157 5541 5809
Size: 711 151 133 158 244 1128 1384 268
Cuts at: 5809 1632
Size: 1917
Fragments arranged by size:
1917 1384 1128 711 268 244 158 151
133
AfeI AGC'GCT
Cuts at: 2147 3260 2147
Size: 1113 4981
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2301 4993 5970 1203
Size: 1098 2692 977 1327
Fragments arranged by size:
2692 1327 1098 977
AgeI A'CCGG_T
Cuts at: 2307 4925 2307
Size: 2618 3476
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 2428 3190 3837 5409 2428
Size: 762 647 1572 3113
Fragments arranged by size:
3113 1572 762 647
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3239 3403 3629 3781 5890 1493
Size: 1746 164 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 226 164 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 3129 3419 1870
Size: 73 105 1081 290 4545
Fragments arranged by size:
4545 1081 290 105 73
BamHI G'GATC_C
Cuts at: 2161 3424 3791 2161
Size: 1263 367 4464
Fragments arranged by size:
4464 1263 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2084 2265 3415 4870
Size: 325 217 627 225 72 181 1150 1455
Cuts at: 4870 618
Size: 1842
Fragments arranged by size:
1842 1455 1150 627 325 225 217 181
72
BanII G_rGCy'C
Cuts at: 1391 2655 3168 3413 1391
Size: 1264 513 245 4072
Fragments arranged by size:
4072 1264 513 245
BbeI G_GCGC'C
Cuts at: 2016 2016
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 928
Size: 971 679 4444
Fragments arranged by size:
4444 971 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2121 2155 4285 4319 1903
Size: 34 184 34 2130 34 3678
Fragments arranged by size:
3678 2130 184 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3010 3986 5196 3010
Size: 976 1210 3908
Fragments arranged by size:
3908 1210 976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4705 136
Size: 107 122 71 631 605 3033 1525
Fragments arranged by size:
3033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 4155 5311 1790
Size: 2365 1156 2573
Fragments arranged by size:
2573 2365 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3825 4783 442
Size: 416 53 83 399 24 2408 958 1753
Fragments arranged by size:
2408 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 2383 4754 1239
Size: 1096 48 2371 2579
Fragments arranged by size:
2579 2371 1096 48
Bpu10I CC'TnA_GC
Cuts at: 3332 3332
Size: 6094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3290 3296 4219 5084 5382 3290
Size: 6 923 865 298 4002
Fragments arranged by size:
4002 923 865 298 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 882
Size: 617 3258 2219
Fragments arranged by size:
3258 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2302 2812 3885 493
Size: 711 1098 510 1073 2702
Fragments arranged by size:
2702 1098 1073 711 510
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2134 3901 4283
Size: 53 83 186 936 480 121 1767 382
Cuts at: 4283 4918 275
Size: 635 1451
Fragments arranged by size:
1767 1451 936 635 480 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 2193 2307 4527 4925 5199 5346 5757 2193
Size: 114 2220 398 274 147 411 2530
Fragments arranged by size:
2530 2220 411 398 274 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2735 2765 1178
Size: 30 165 30 1047 30 255 30 4507
Fragments arranged by size:
4507 1047 255 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2432 2492 2645 1186
Size: 1213 33 60 153 4635
Fragments arranged by size:
4635 1213 153 60 33
BseYI C'CCAG_C
Cuts at: 2631 2823 3082 3252 5297 5793 2631
Size: 192 259 170 2045 496 2932
Fragments arranged by size:
2932 2045 496 259 192 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2299 2504 2509 1163
Size: 573 396 167 205 5 4748
Fragments arranged by size:
4748 573 396 205 167 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3126 3168 3276 4155 4240 5311 3126
Size: 42 108 879 85 1071 3909
Fragments arranged by size:
3909 1071 879 108 85 42
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 2099
Size: 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2655 3126 3168 3276 3413 4155 4240
Size: 399 865 471 42 108 137 742 85
Cuts at: 4240 5311 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 865 742 471 399 137 108
85 42
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3091 3983 5713 3091
Size: 892 1730 3472
Fragments arranged by size:
3472 1730 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2526 3449 844
Size: 1433 249 923 3489
Fragments arranged by size:
3489 1433 923 249
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2798 4589 4763 65
Size: 1183 1550 1791 174 1396
Fragments arranged by size:
1791 1550 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2066 2307 4738 4925 1700
Size: 110 199 57 241 2431 187 2869
Fragments arranged by size:
2869 2431 241 199 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 2757 3363 96
Size: 2661 606 2827
Fragments arranged by size:
2827 2661 606
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 2648
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 2161 2796 3424 3791 4176 4193 5645 5731
Size: 189 635 628 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 635 628 385 367 346 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3345 513
Size: 552 939 1341 3262
Fragments arranged by size:
3262 1341 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 2290 2521 2762 3343 3610 4404 4424 2290
Size: 231 241 581 267 794 20 3960
Fragments arranged by size:
3960 794 581 267 241 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2219 2718
Size: 54 1425 50 275 18 177 212 499
Cuts at: 2718 3198 3541 4430 6059 8
Size: 480 343 889 1629 43
Fragments arranged by size:
1629 1425 889 499 480 343 275 212
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3242 4024 1353
Size: 1889 782 3423
Fragments arranged by size:
3423 1889 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3166 3166
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2335 2343 2383 2494 2627 2785 3029
Size: 393 703 8 40 111 133 158 244
Cuts at: 3029 4157 4754 5541 5809 1239
Size: 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 703 597 393 268 244
158 133 111 40 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2876 2156
Size: 720 5374
EcoO109I rG'GnC_Cy
Cuts at: 3135 3135
Size: 6094
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FseI GG_CCGG'CC
Cuts at: 2070 2070
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2016 2149 3262 5241 1678
Size: 338 133 1113 1979 2531
Fragments arranged by size:
2531 1979 1113 338 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 4735 4767 4809
Size: 32 135 32 574 32 2557 32 42
Cuts at: 4809 4841 1373
Size: 32 2626
Fragments arranged by size:
2626 2557 574 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3220 3438 3684 669
Size: 988 281 1282 218 246 3079
Fragments arranged by size:
3079 1282 988 281 246 218
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 2012 2012
Size: 6094
KpnI G_GTAC'C
Cuts at: 2269 3419 2269
Size: 1150 4944
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 2931 5208 5392 1305
Size: 1050 576 2277 184 2007
Fragments arranged by size:
2277 2007 1050 576 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2221 3200 10
Size: 54 1425 343 389 979 2904
Fragments arranged by size:
2904 1425 979 389 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 4052 4411 4570 518
Size: 648 1791 1095 359 159 2042
Fragments arranged by size:
2042 1791 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2256 2321 3235 3510 4187 5335 5580
Size: 420 230 65 914 275 677 1148 245
Cuts at: 5580 1606
Size: 2120
Fragments arranged by size:
2120 1148 914 677 420 275 245 230
65
NaeI GCC'GGC
Cuts at: 2011 2068 2011
Size: 57 6037
NarI GG'CG_CC
Cuts at: 2013 2013
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NgoMIV G'CCGG_C
Cuts at: 2009 2066 2009
Size: 57 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 3364 3452 4997 1132
Size: 97 2135 88 1545 2229
Fragments arranged by size:
2229 2135 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 2080 2274 2853 1348
Size: 732 194 579 4589
Fragments arranged by size:
4589 732 579 194
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PmlI CAC'GTG
Cuts at: 2302 2302
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3135 3135
Size: 6094
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PstI C_TGCA'G
Cuts at: 830 2340 3392 3446 830
Size: 1510 1052 54 3478
Fragments arranged by size:
3478 1510 1052 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2256 2321 3235 2256
Size: 65 914 5115
Fragments arranged by size:
5115 914 65
SacI G_AGCT'C
Cuts at: 3168 3168
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SanDI GG'GwC_CC
Cuts at: 3135 3135
Size: 6094
SapI GCTCTTCn'nnn_
Cuts at: 3242 3242
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 2708 4342 6054 2708
Size: 1634 1712 2748
Fragments arranged by size:
2748 1712 1634
SfcI C'TryA_G
Cuts at: 826 1045 2336 3388 3442 4577 5258 5449 826
Size: 219 1291 1052 54 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 1052 681 219 191 54
SfoI GGC'GCC
Cuts at: 2014 2014
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3131 3421 1872
Size: 1259 290 4545
Fragments arranged by size:
4545 1259 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3269 3311 4198 5099 5361 820
Size: 197 926 1326 42 887 901 262 1553
Fragments arranged by size:
1553 1326 926 901 887 262 197 42
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2328 3383 1517
Size: 811 1055 4228
Fragments arranged by size:
4228 1055 811
StyI C'CwwG_G
Cuts at: 513 1065 2004 3533 513
Size: 552 939 1529 3074
Fragments arranged by size:
3074 1529 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2767 4121 4138 4291 4476 4921 325
Size: 1255 1187 1354 17 153 185 445 1498
Fragments arranged by size:
1498 1354 1255 1187 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 2757 3363
Size: 274 80 33 51 1443 729 51 606
Cuts at: 3363 4340 6052 96
Size: 977 1712 138
Fragments arranged by size:
1712 1443 977 729 606 274 138 80
51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3129 3419 1870
Size: 1259 290 4545
Fragments arranged by size:
4545 1259 290
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SrfI SwaI XcmI