Restriction map for
pFN21AB7194
(Circular) (Six-base) MAPSORT of: pFN21AB7194.seq Check: 8416 from: 1 to: 6733
pFN21AB7194 6733 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2653 2653
Size: 6733
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3627 4543 278
Size: 53 83 186 936 2091 916 2468
Fragments arranged by size:
2468 2091 936 916 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2727 3378 3948 4076 1937
Size: 397 393 651 570 128 4594
Fragments arranged by size:
4594 651 570 397 393 128
Acc65I G'GTAC_C
Cuts at: 4054 4054
Size: 6733
AclI AA'CG_TT
Cuts at: 1563 4860 5233 1563
Size: 3297 373 3063
Fragments arranged by size:
3297 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2404 3083 4796 6180 6448 1632
Size: 772 679 1713 1384 268 1917
Fragments arranged by size:
1917 1713 1384 772 679 268
AfeI AGC'GCT
Cuts at: 2045 2045
Size: 6733
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6536
AflIII A'CryG_T
Cuts at: 1203 2722 5632 6609 1203
Size: 1519 2910 977 1327
Fragments arranged by size:
2910 1519 1327 977
AgeI A'CCGG_T
Cuts at: 5564 5564
Size: 6733
AhdI GACnn_n'nnGTC
Cuts at: 5462 5462
Size: 6733
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2685 3160 3995 1184
Size: 1501 475 835 3922
Fragments arranged by size:
3922 1501 835 475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 6701
AlwNI CAG_nnn'CTG
Cuts at: 2710 2788 3820 4476 6048 2710
Size: 78 1032 656 1572 3395
Fragments arranged by size:
3395 1572 1032 656 78
ApaI G_GGCC'C
Cuts at: 3913 4019 3913
Size: 106 6627
ApaLI G'TGCA_C
Cuts at: 4790 5946 4790
Size: 1156 5577
ApoI r'AATT_y
Cuts at: 1493 3587 4042 4268 4420 6529 1493
Size: 2094 455 226 152 2109 1697
Fragments arranged by size:
2109 2094 1697 455 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5287 160
Size: 872 4255 1606
Fragments arranged by size:
4255 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
AvaI C'yCGr_G
Cuts at: 1870 1943 2345 3049 4058 1870
Size: 73 402 704 1009 4545
Fragments arranged by size:
4545 1009 704 402 73
BamHI G'GATC_C
Cuts at: 4063 4430 4063
Size: 367 6366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2174 2741 2959 3770
Size: 325 217 627 282 105 567 218 811
Cuts at: 3770 4054 5509 618
Size: 284 1455 1842
Fragments arranged by size:
1842 1455 811 627 567 325 284 282
218 217 105
BanII G_rGCy'C
Cuts at: 1391 2654 2762 3410 3913 3960 4019 4052 1391
Size: 1263 108 648 503 47 59 33 4072
Fragments arranged by size:
4072 1263 648 503 108 59 47 33
BbeI G_GCGC'C
Cuts at: 2073 2178 3774 2073
Size: 105 1596 5032
Fragments arranged by size:
5032 1596 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2383 2761 2987 3798 928
Size: 971 484 378 226 811 3863
Fragments arranged by size:
3863 971 811 484 378 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2429 2463 3748 3782 4924 4958 1903
Size: 34 492 34 1285 34 1142 34 3678
Fragments arranged by size:
3678 1285 1142 492 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4625 5835 4625
Size: 1210 5523
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6733
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2142 2456
Size: 107 122 71 631 605 395 75 314
Cuts at: 2456 2678 2739 5344 136
Size: 222 61 2605 1525
Fragments arranged by size:
2605 1525 631 605 395 314 222 122
107 75 71 61
BglII A'GATC_T
Cuts at: 3183 6728 3183
Size: 3545 3188
BlpI GC'TnA_GC
Cuts at: 4150 4150
Size: 6733
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2964 3913 4008 4019 4794 5950 1790
Size: 318 856 949 95 11 775 1156 2573
Fragments arranged by size:
2573 1156 949 856 775 318 95 11
BmgBI CAC'GTC
Cuts at: 1318 2320 2676 2725 2970 1318
Size: 1002 356 49 245 5081
Fragments arranged by size:
5081 1002 356 245 49
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2219 4464 5422
Size: 416 53 83 399 24 802 2245 958
Cuts at: 5422 442
Size: 1753
Fragments arranged by size:
2245 1753 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3231 3655 3231
Size: 424 6309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2767 3324 5393 1239
Size: 899 629 557 2069 2579
Fragments arranged by size:
2579 2069 899 629 557
Bpu10I CC'TnA_GC
Cuts at: 3954 3954
Size: 6733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4858 5723 6021 4858
Size: 865 298 5570
Fragments arranged by size:
5570 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5396 882
Size: 617 3897 2219
Fragments arranged by size:
3897 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2518 2557 3336 4524 493
Size: 711 1314 39 779 1188 2702
Fragments arranged by size:
2702 1314 1188 779 711 39
BsaBI GATnn'nnATC
Cuts at: 2483 3188 6727 2483
Size: 705 3539 2489
Fragments arranged by size:
3539 2489 705
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2175 3624 3771
Size: 53 83 186 936 537 105 1449 147
Cuts at: 3771 4540 4922 5557 275
Size: 769 382 635 1451
Fragments arranged by size:
1451 1449 936 769 635 537 382 186
147 105 83 53
BsaWI w'CCGG_w
Cuts at: 3192 5166 5564 5838 5985 6396 3192
Size: 1974 398 274 147 411 3529
Fragments arranged by size:
3529 1974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3152 3182 1178
Size: 30 165 30 1749 30 4729
Fragments arranged by size:
4729 1749 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 2821 3160 3700 1186
Size: 822 813 339 540 4219
Fragments arranged by size:
4219 822 813 540 339
BseYI C'CCAG_C
Cuts at: 2046 3031 3227 3921 3968 5936 6432 2046
Size: 985 196 694 47 1968 496 2347
Fragments arranged by size:
2347 1968 985 694 496 196 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3089 1163
Size: 573 1353 4807
Fragments arranged by size:
4807 1353 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2208 2252 2731 3768 4183 4944
Size: 275 186 207 44 479 1037 415 761
Cuts at: 4944 5972 1540
Size: 1028 2301
Fragments arranged by size:
2301 1037 1028 761 479 415 275 207
186 44
BsiHKAI G_wGCw'C
Cuts at: 3960 4794 4879 5950 3960
Size: 834 85 1071 4743
Fragments arranged by size:
4743 1071 834 85
BsmI GAATG_Cn'
Cuts at: 4244 4337 4244
Size: 93 6640
BsmBI CGTCTCn'nnnn_
Cuts at: 2281 3736 2281
Size: 1455 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2654 2762 2964 3410 3913 3960
Size: 399 318 546 108 202 446 503 47
Cuts at: 3960 4008 4019 4052 4794 4879 5950 1391
Size: 48 11 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 503 446 399 318
202 108 85 48 47 33 11
BspEI T'CCGG_A
Cuts at: 3192 6396 3192
Size: 3204 3529
BspHI T'CATG_A
Cuts at: 3441 4622 6352 3441
Size: 1181 1730 3822
Fragments arranged by size:
3822 1730 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2653 4088 844
Size: 1809 1435 3489
Fragments arranged by size:
3489 1809 1435
BsrBI CCG'CTC
Cuts at: 1394 1823 3355 4620 1394
Size: 429 1532 1265 3507
Fragments arranged by size:
3507 1532 1265 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5228 5402 65
Size: 1183 3980 174 1396
Fragments arranged by size:
3980 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2254 2357 2537 3099 5377 5564 1700
Size: 110 444 103 180 562 2278 187 2869
Fragments arranged by size:
2869 2278 562 444 187 180 110 103
BsrGI T'GTAC_A
Cuts at: 96 2802 96
Size: 2706 4027
BssHII G'CGCG_C
Cuts at: 1925 2148 2309 1925
Size: 223 161 6349
Fragments arranged by size:
6349 223 161
BssSI C'ACGA_G
Cuts at: 4793 5805 4793
Size: 1012 5721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6733
BstBI TT'CG_AA
Cuts at: 5537 5537
Size: 6733
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6733
BstXI CCAn_nnnn'nTGG
Cuts at: 3704 3704
Size: 6733
BstYI r'GATC_y
Cuts at: 1972 2706 3105 3183 4063 4430 4815 4832 6284
Size: 734 399 78 880 367 385 17 1452
Cuts at: 6284 6370 6382 6728 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 880 734 399 385 367 346
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2711 2711
Size: 6733
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2246 2460 2631 2763 513
Size: 552 939 6 236 214 171 132 4483
Fragments arranged by size:
4483 939 552 236 214 171 132 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2149 2971 487
Size: 683 56 923 822 4249
Fragments arranged by size:
4249 923 822 683 56
BtsI GCAGTG_nn'
Cuts at: 3413 3940 4249 5043 5063 3413
Size: 527 309 794 20 5083
Fragments arranged by size:
5083 794 527 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6733
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6698
DraI TTT'AAA
Cuts at: 4037 4037
Size: 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2273 5740 809
Size: 507 957 3467 1802
Fragments arranged by size:
3467 1802 957 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2094 2205
Size: 54 1425 50 275 18 177 87 111
Cuts at: 2205 2249 2457 2679 3576 4180 5069 6698 8
Size: 44 208 222 897 604 889 1629 43
Fragments arranged by size:
1629 1425 897 889 604 275 222 208
177 111 87 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2205 2249 4180 1537
Size: 275 393 44 1931 4090
Fragments arranged by size:
4090 1931 393 275 44
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 3345 3398 3965 4663 1353
Size: 1124 868 53 567 698 3423
Fragments arranged by size:
3423 1124 868 698 567 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2141 2513 2524 5320 5694 5840
Size: 1064 138 552 372 11 2796 374 146
Cuts at: 5840 387
Size: 1280
Fragments arranged by size:
2796 1280 1064 552 374 372 146 138
11
EcoICRI GAG'CTC
Cuts at: 3958 3958
Size: 6733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2404 2767 3083 3324 4796 5393
Size: 393 506 266 363 316 241 1472 597
Cuts at: 5393 6180 6448 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1472 787 597 506 393 363 316
268 266 241
EcoO109I rG'GnC_Cy
Cuts at: 2183 3199 3466 3909 4015 4016 2183
Size: 1016 267 443 106 1 4900
Fragments arranged by size:
4900 1016 443 267 106 1
EcoRI G'AATT_C
Cuts at: 4042 4420 4042
Size: 378 6355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 6733
FspI TGC'GCA
Cuts at: 3668 5239 3668
Size: 1571 5162
FspAI rTGC'GCAy
Cuts at: 3668 3668
Size: 6733
HaeII r_GCGC'y
Cuts at: 1678 2047 2073 2178 3774 5880 1678
Size: 369 26 105 1596 2106 2531
Fragments arranged by size:
2531 2106 1596 369 105 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3356 3388 3733 3765 4013
Size: 32 135 32 1784 32 345 32 248
Cuts at: 4013 4045 5374 5406 5448 5480 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1784 1329 345 248 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 3379 3949 4077 4323 669
Size: 988 281 790 651 570 128 246 3079
Fragments arranged by size:
3079 988 790 651 570 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6733
HpaI GTT'AAC
Cuts at: 4323 4323
Size: 6733
KasI G'GCGC_C
Cuts at: 2069 2174 3770 2069
Size: 105 1596 5032
Fragments arranged by size:
5032 1596 105
KpnI G_GTAC'C
Cuts at: 4058 4058
Size: 6733
MfeI C'AATT_G
Cuts at: 4332 4332
Size: 6733
MluI A'CGCG_T
Cuts at: 6609 6609
Size: 6733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 5847 6031 1305
Size: 954 3588 184 2007
Fragments arranged by size:
3588 2007 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2459 3578 10
Size: 54 1425 343 264 363 1119 3165
Fragments arranged by size:
3165 1425 1119 363 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3142 3836 4691 5050 5209 518
Size: 648 1976 694 855 359 159 2042
Fragments arranged by size:
2042 1976 855 694 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2248 3035 3520 3808 3850 3885 3997
Size: 406 236 787 485 288 42 35 112
Cuts at: 3997 4149 4826 5974 6219 1606
Size: 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 787 677 485 406 288 245
236 152 112 42 35
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 6733
NarI GG'CG_CC
Cuts at: 2070 2175 3771 2070
Size: 105 1596 5032
Fragments arranged by size:
5032 1596 105
NcoI C'CATG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 4615
Fragments arranged by size:
4615 939 627 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6733
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 6733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6733
NotI GC'GGCC_GC
Cuts at: 2249 4180 2249
Size: 1931 4802
NspI r_CATG'y
Cuts at: 1132 1229 3433 3565 3841 4091 5636 1132
Size: 97 2204 132 276 250 1545 2229
Fragments arranged by size:
2229 2204 1545 276 250 132 97
PasI CC'CwG_GG
Cuts at: 1348 2028 1348
Size: 680 6053
PciI A'CATG_T
Cuts at: 5632 5632
Size: 6733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2361 3820 1108
Size: 1253 1459 4021
Fragments arranged by size:
4021 1459 1253
PfoI T'CCnGG_A
Cuts at: 3301 3301
Size: 6733
PmeI GTTT'AAAC
Cuts at: 4037 4037
Size: 6733
PmlI CAC'GTG
Cuts at: 3336 3336
Size: 6733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 4802 4834 6315 6347 3152
Size: 32 1618 32 1481 32 3538
Fragments arranged by size:
3538 1618 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2183 3466 2183
Size: 1283 5450
PshAI GACnn'nnGTC
Cuts at: 1613 2972 1613
Size: 1359 5374
PsiI TTA'TAA
Cuts at: 4303 4303
Size: 6733
PspOMI G'GGCC_C
Cuts at: 3909 4015 3909
Size: 106 6627
PspXI vC'TCGA_Gb
Cuts at: 2345 2345
Size: 6733
PstI C_TGCA'G
Cuts at: 830 2706 3684 4085 830
Size: 1876 978 401 3478
Fragments arranged by size:
3478 1876 978 401
PvuI CG_AT'CG
Cuts at: 2001 3768 2001
Size: 1767 4966
PvuII CAG'CTG
Cuts at: 3520 3808 3850 3997 3520
Size: 288 42 147 6256
Fragments arranged by size:
6256 288 147 42
SacI G_AGCT'C
Cuts at: 3960 3960
Size: 6733
SacII CC_GC'GG
Cuts at: 2013 2249 2013
Size: 236 6497
SalI G'TCGA_C
Cuts at: 1936 2726 3377 3947 4075 1936
Size: 790 651 570 128 4594
Fragments arranged by size:
4594 790 651 570 128
SapI GCTCTTCn'nnn_
Cuts at: 3345 3398 3965 3345
Size: 53 567 6113
Fragments arranged by size:
6113 567 53
SbfI CC_TGCA'GG
Cuts at: 4085 4085
Size: 6733
ScaI AGT'ACT
Cuts at: 3569 3638 4981 6693 3569
Size: 69 1343 1712 3609
Fragments arranged by size:
3609 1712 1343 69
SfcI C'TryA_G
Cuts at: 826 1045 2284 2702 3680 4081 5216 5897 6088
Size: 219 1239 418 978 401 1135 681 191
Cuts at: 6088 826
Size: 1471
Fragments arranged by size:
1471 1239 1135 978 681 418 401 219
191
SfoI GGC'GCC
Cuts at: 2071 2176 3772 2071
Size: 105 1596 5032
Fragments arranged by size:
5032 1596 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
SgrAI Cr'CCGG_yG
Cuts at: 1700 2254 1700
Size: 554 6179
SmaI CCC'GGG
Cuts at: 1872 4060 1872
Size: 2188 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 4837 5738 6000 820
Size: 197 926 402 2492 901 262 1553
Fragments arranged by size:
2492 1553 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4524 493
Size: 4031 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6733
SphI G_CATG'C
Cuts at: 1132 3433 3565 4091 1132
Size: 2301 132 526 3774
Fragments arranged by size:
3774 2301 526 132
SspI AAT'ATT
Cuts at: 5 52 4657 5
Size: 47 4605 2081
Fragments arranged by size:
4605 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6733
StyI C'CwwG_G
Cuts at: 513 1065 2004 2239 2631 2655 4172 513
Size: 552 939 235 392 24 1517 3074
Fragments arranged by size:
3074 1517 939 552 392 235 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3241 3514 3521 3935 4760 4777
Size: 1255 750 911 273 7 414 825 17
Cuts at: 4777 4930 5115 5560 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 911 825 750 445 414 273
185 153 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 3567 3636
Size: 274 80 33 51 1443 825 765 69
Cuts at: 3636 4979 6691 96
Size: 1343 1712 138
Fragments arranged by size:
1712 1443 1343 825 765 274 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6693 6725 6693
Size: 32 6701
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6733
XbaI T'CTAG_A
Cuts at: 4069 4069
Size: 6733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3247 3247
Size: 6733
XhoI C'TCGA_G
Cuts at: 1943 2345 1943
Size: 402 6331
XmaI C'CCGG_G
Cuts at: 1870 4058 1870
Size: 2188 4545
XmnI GAAnn'nnTTC
Cuts at: 2369 3092 3587 4862 2369
Size: 723 495 1275 4240
Fragments arranged by size:
4240 1275 723 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3625 4541 276
Size: 53 83 186 936 2091 916 2468
Fragments arranged by size:
2468 2091 936 916 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII
EcoNI EcoRV FseI NruI NsiI PacI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI