Restriction map for
pFN21AB7188
(Circular) (Six-base) MAPSORT of: pFN21AB7188.seq Check: 9113 from: 1 to: 6091
pFN21AB7188 6091 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2823 2823
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4154 5538 5806 1632
Size: 2522 1384 268 1917
Fragments arranged by size:
2522 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3263 3834 5406 3263
Size: 571 1572 3948
Fragments arranged by size:
3948 1572 571
ApaLI G'TGCA_C
Cuts at: 2618 2999 4148 5304 2618
Size: 381 1149 1156 3405
Fragments arranged by size:
3405 1156 1149 381
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2602 2663 2858 2869 2875 2907
Size: 73 119 540 61 195 11 6 32
Cuts at: 2907 2935 3416 1870
Size: 28 481 4545
Fragments arranged by size:
4545 540 481 195 119 73 61 32
28 11 6
AvrII C'CTAG_G
Cuts at: 2170 2170
Size: 6091
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2174 2636 2744 2827
Size: 325 217 627 319 68 462 108 83
Cuts at: 2827 3032 3059 3175 3332 3412 4867 618
Size: 205 27 116 157 80 1455 1842
Fragments arranged by size:
1842 1455 627 462 325 319 217 205
157 116 108 83 80 68 27
BanII G_rGCy'C
Cuts at: 1391 2609 2663 2859 2876 3410 1391
Size: 1218 54 196 17 534 4072
Fragments arranged by size:
4072 1218 534 196 54 17
BbeI G_GCGC'C
Cuts at: 2178 2748 2831 3036 3179 2178
Size: 570 83 205 143 5090
Fragments arranged by size:
5090 570 205 143 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2749 928
Size: 971 272 578 4270
Fragments arranged by size:
4270 971 578 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2008 2021 2042 2074 2108 2553
Size: 34 50 21 13 21 32 34 445
Cuts at: 2553 2587 4282 4316 1903
Size: 34 1695 34 3678
Fragments arranged by size:
3678 1695 445 50 34 34 34 34
32 21 21 13
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 4702 136
Size: 107 122 71 631 605 823 2207 1525
Fragments arranged by size:
2207 1525 823 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2199 6086 2199
Size: 3887 2204
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2622 3003 3062 4152 5308 1790
Size: 832 381 59 1090 1156 2573
Fragments arranged by size:
2573 1156 1090 832 381 59
BmgBI CAC'GTC
Cuts at: 1318 2454 1318
Size: 1136 4955
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 2909 3822
Size: 416 53 83 399 24 1110 382 913
Cuts at: 3822 4780 442
Size: 958 1753
Fragments arranged by size:
1753 1110 958 913 416 399 382 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2645 3240 4751 1239
Size: 949 457 595 1511 2579
Fragments arranged by size:
2579 1511 949 595 457
Bpu10I CC'TnA_GC
Cuts at: 2657 3014 2657
Size: 357 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 2953 3882 493
Size: 711 1560 189 929 2702
Fragments arranged by size:
2702 1560 929 711 189
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2745 2828 2864
Size: 53 83 186 936 642 570 83 36
Cuts at: 2864 3033 3176 3898 4280 4915 275
Size: 169 143 722 382 635 1451
Fragments arranged by size:
1451 936 722 642 635 570 382 186
169 143 83 83 53 36
BsaWI w'CCGG_w
Cuts at: 2279 4524 4922 5196 5343 5754 2279
Size: 2245 398 274 147 411 2616
Fragments arranged by size:
2616 2245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2116 2426 3035 3122 1186
Size: 849 81 310 609 87 4155
Fragments arranged by size:
4155 849 609 310 87 81
BseYI C'CCAG_C
Cuts at: 2385 3117 3367 5294 5790 2385
Size: 732 250 1927 496 2686
Fragments arranged by size:
2686 1927 732 496 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 3153 3344 1163
Size: 573 669 748 191 3910
Fragments arranged by size:
3910 748 669 573 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2467 3541 4302 5330 1540
Size: 275 186 466 1074 761 1028 2301
Fragments arranged by size:
2301 1074 1028 761 466 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 3003 3114 4152 4237 5308 2622
Size: 381 111 1038 85 1071 3405
Fragments arranged by size:
3405 1071 1038 381 111 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
BsmBI CGTCTCn'nnnn_
Cuts at: 2047 2765 2047
Size: 718 5373
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2609 2622 2663 2859 2876 3003 3062
Size: 399 819 13 41 196 17 127 59
Cuts at: 3062 3114 3410 4152 4237 5308 1391
Size: 52 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 819 742 399 296 196 127
85 59 52 41 17 13
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 3446 844
Size: 1979 623 3489
Fragments arranged by size:
3489 1979 623
BsrBI CCG'CTC
Cuts at: 1394 1823 2285 3046 3254 3978 1394
Size: 429 462 761 208 724 3507
Fragments arranged by size:
3507 761 724 462 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2507 2697 2979 4735 4922 1700
Size: 110 697 190 282 1756 187 2869
Fragments arranged by size:
2869 1756 697 282 190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 3182 1925
Size: 1257 4834
BssSI C'ACGA_G
Cuts at: 3088 4151 5163 3088
Size: 1063 1012 4016
Fragments arranged by size:
4016 1063 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2374 1184
Size: 1190 4901
BstYI r'GATC_y
Cuts at: 1972 2199 3421 3788 4173 4190 5642 5728 5740
Size: 227 1222 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1222 385 367 346 227 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2613 3027 3193 3220 3291 513
Size: 552 1548 414 166 27 71 3313
Fragments arranged by size:
3313 1548 552 414 166 71 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2548 3607 4401 4421 2445
Size: 103 1059 794 20 4115
Fragments arranged by size:
4115 1059 794 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DraIII CAC_nnn'GTG
Cuts at: 3007 3007
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2331 2354
Size: 54 1425 50 275 18 327 174 23
Cuts at: 2354 2398 2580 2610 2844 2958 3538 4427 6056
Size: 44 182 30 234 114 580 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1629 1425 889 580 327 275 234 182
174 114 54 50 44 43 30 23
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 4021 1353
Size: 740 1928 3423
Fragments arranged by size:
3423 1928 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2699 3134 4678 5052 5198 387
Size: 1064 138 1110 435 1544 374 146 1280
Fragments arranged by size:
1544 1280 1110 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2645 3240 4154 4751 5538 5806
Size: 393 556 457 595 914 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 914 787 597 595 556 457 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 3017 2627
Size: 390 5701
EcoO109I rG'GnC_Cy
Cuts at: 2653 2903 3355 2653
Size: 250 452 5389
Fragments arranged by size:
5389 452 250
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 6091
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2178 2748 2794 2831 2915 3036 3179 5238
Size: 500 570 46 37 84 121 143 2059
Cuts at: 5238 1678
Size: 2531
Fragments arranged by size:
2531 2059 570 500 143 121 84 46
37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373
Size: 32 135 32 3160 32 42 32 2626
Fragments arranged by size:
3160 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2174 2744 2827 3032 3175 2174
Size: 570 83 205 143 5090
Fragments arranged by size:
5090 570 205 143 83
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 2356 2400 10
Size: 54 1425 343 501 23 44 3701
Fragments arranged by size:
3701 1425 501 343 54 44 23
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 4049 4408 4567 518
Size: 648 1173 1710 359 159 2042
Fragments arranged by size:
2042 1710 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2996 3029 3117 3195 3207 3222 3293
Size: 575 815 33 88 78 12 15 71
Cuts at: 3293 3316 3367 3507 4184 5332 5577 1606
Size: 23 51 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 575 245 140 88
78 71 51 33 23 15 12
NaeI GCC'GGC
Cuts at: 2509 2699 2981 2509
Size: 190 282 5619
Fragments arranged by size:
5619 282 190
NarI GG'CG_CC
Cuts at: 2175 2745 2828 3033 3176 2175
Size: 570 83 205 143 5090
Fragments arranged by size:
5090 570 205 143 83
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 2507 2697 2979 2507
Size: 190 282 5619
Fragments arranged by size:
5619 282 190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2485 3449 4994 1132
Size: 97 1256 964 1545 2229
Fragments arranged by size:
2229 1545 1256 964 97
PasI CC'CwG_GG
Cuts at: 1348 3055 1348
Size: 1707 4384
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2727 3342 2727
Size: 615 5476
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2653
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 3117 3316 3117
Size: 199 5892
RsrII CG'GwC_CG
Cuts at: 2078 3190 2078
Size: 1112 4979
SacII CC_GC'GG
Cuts at: 3030 3196 3223 3294 3030
Size: 166 27 71 5827
Fragments arranged by size:
5827 166 71 27
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2707 3439 4574 5255 5446 826
Size: 219 1662 732 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 732 681 219 191
SfoI GGC'GCC
Cuts at: 2176 2746 2829 3034 3177 2176
Size: 570 83 205 143 5090
Fragments arranged by size:
5090 570 205 143 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 2604 2665 2937 3418 1872
Size: 732 61 272 481 4545
Fragments arranged by size:
4545 732 481 272 61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2485 3449 1132
Size: 1353 964 3774
Fragments arranged by size:
3774 1353 964
SrfI GCCC'GGGC
Cuts at: 2937 2937
Size: 6091
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2170 2535 3530 513
Size: 552 1105 365 995 3074
Fragments arranged by size:
3074 1105 995 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2393 2509 4118 4135 4288 4473 4918
Size: 1255 813 116 1609 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1609 1498 1255 813 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4337 6049
Size: 274 80 33 51 1443 546 1814 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1814 1712 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2620 2011
Size: 609 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 2602 2663 2935 3416 1870
Size: 732 61 272 481 4545
Fragments arranged by size:
4545 732 481 272 61
XmnI GAAnn'nnTTC
Cuts at: 2756 4220 2756
Size: 1464 4627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI SacI SanDI
SapI SexAI SfiI SwaI