Restriction map for
pFN21AB7187
(Circular) (Six-base) MAPSORT of: pFN21AB7187.seq Check: 7486 from: 1 to: 6322
pFN21AB7187 6322 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3186 3186
Size: 6322
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4385 5769 6037 1632
Size: 1958 795 1384 268 1917
Fragments arranged by size:
1958 1917 1384 795 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 2207 2498 4065 5637 2207
Size: 291 1567 1572 2892
Fragments arranged by size:
2892 1572 1567 291
ApaI G_GGCC'C
Cuts at: 2030 2937 2030
Size: 907 5415
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 3389 3631 3857 4009 6118 1493
Size: 1896 242 226 152 2109 1697
Fragments arranged by size:
2109 1896 1697 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2405 2936 3647 1870
Size: 73 86 376 531 711 4545
Fragments arranged by size:
4545 711 531 376 86 73
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2294 2594 3643 5098
Size: 325 217 627 446 61 300 1049 1455
Cuts at: 5098 618
Size: 1842
Fragments arranged by size:
1842 1455 1049 627 446 325 300 217
61
BanII G_rGCy'C
Cuts at: 1391 2030 2492 2519 2607 2937 3287 3411 3452
Size: 639 462 27 88 330 350 124 41
Cuts at: 3452 3641 1391
Size: 189 4072
Fragments arranged by size:
4072 639 462 350 330 189 124 88
41 27
BbeI G_GCGC'C
Cuts at: 2237 2237
Size: 6322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3596 928
Size: 971 1697 3654
Fragments arranged by size:
3654 1697 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2995 3029 4513 4547 1903
Size: 34 1058 34 1484 34 3678
Fragments arranged by size:
3678 1484 1058 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2099 3087
Size: 107 122 71 631 605 418 9 988
Cuts at: 3087 4933 136
Size: 1846 1525
Fragments arranged by size:
1846 1525 988 631 605 418 122 107
71 9
BglII A'GATC_T
Cuts at: 2674 6317 2674
Size: 3643 2679
BlpI GC'TnA_GC
Cuts at: 2486 3739 2486
Size: 1253 5069
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2597 2913 2937 4383 5539 1790
Size: 240 567 316 24 1446 1156 2573
Fragments arranged by size:
2573 1446 1156 567 316 240 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2925 4053 5011
Size: 416 53 83 399 24 1508 1128 958
Cuts at: 5011 442
Size: 1753
Fragments arranged by size:
1753 1508 1128 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2621 2767 3043 4982 1239
Size: 1130 252 146 276 1939 2579
Fragments arranged by size:
2579 1939 1130 276 252 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2547 3338 3485 3581 4447 5312 5610 2547
Size: 791 147 96 866 865 298 3259
Fragments arranged by size:
3259 866 865 791 298 147 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 2874 4985 882
Size: 617 1230 145 2111 2219
Fragments arranged by size:
2219 2111 1230 617 145
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2222 2234 4129 4511
Size: 53 83 186 936 689 12 1895 382
Cuts at: 4511 5146 275
Size: 635 1451
Fragments arranged by size:
1895 1451 936 689 635 382 186 83
53 12
BsaWI w'CCGG_w
Cuts at: 3393 4755 5153 5427 5574 5985 3393
Size: 1362 398 274 147 411 3730
Fragments arranged by size:
3730 1362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3041 3071 1178
Size: 30 165 30 1638 30 4429
Fragments arranged by size:
4429 1638 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 2852 2855 2864 3533 3536 1186
Size: 1160 506 3 9 669 3 3972
Fragments arranged by size:
3972 1160 669 506 9 3 3
BseYI C'CCAG_C
Cuts at: 2207 2491 5525 6021 2207
Size: 284 3034 496 2508
Fragments arranged by size:
3034 2508 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5749
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3411 3478 4383 4468 5539 3411
Size: 67 905 85 1071 4194
Fragments arranged by size:
4194 1071 905 85 67
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2492 2519 2597 2607 2913 2937
Size: 399 240 462 27 78 10 306 24
Cuts at: 2937 3287 3411 3452 3478 3641 4383 4468 5539
Size: 350 124 41 26 163 742 85 1071
Cuts at: 5539 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 462 399 350 306 240
163 124 85 78 41 27 26 24
10
BspEI T'CCGG_A
Cuts at: 3393 5985 3393
Size: 2592 3730
BspHI T'CATG_A
Cuts at: 3315 4211 5941 3315
Size: 896 1730 3696
Fragments arranged by size:
3696 1730 896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3186 3357 3677 844
Size: 2342 171 320 3489
Fragments arranged by size:
3489 2342 320 171
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 4817 4991 65
Size: 1183 2190 1379 174 1396
Fragments arranged by size:
2190 1396 1379 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2192 2291 2611 3114 4966 5153
Size: 110 288 94 99 320 503 1852 187
Cuts at: 5153 1700
Size: 2869
Fragments arranged by size:
2869 1852 503 320 288 187 110 99
94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 2396 4382 5394 2396
Size: 1986 1012 3324
Fragments arranged by size:
3324 1986 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3078 794
Size: 2284 4038
BstBI TT'CG_AA
Cuts at: 2643 5126 2643
Size: 2483 3839
BstEII G'GTnAC_C
Cuts at: 1184 2287 2694 3575 1184
Size: 1103 407 881 3931
Fragments arranged by size:
3931 1103 881 407
BstYI r'GATC_y
Cuts at: 1972 2674 3652 4019 4404 4421 5873 5959 5971
Size: 702 978 367 385 17 1452 86 12
Cuts at: 5971 6317 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 978 702 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2436 3020 2436
Size: 584 5738
BtgI C'CryG_G
Cuts at: 513 1065 2004 3279 513
Size: 552 939 1275 3556
Fragments arranged by size:
3556 1275 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2373 2998 487
Size: 683 56 1147 625 3811
Fragments arranged by size:
3811 1147 683 625 56
BtsI GCAGTG_nn'
Cuts at: 2048 3091 3838 4632 4652 2048
Size: 1043 747 794 20 3718
Fragments arranged by size:
3718 1043 794 747 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DraIII CAC_nnn'GTG
Cuts at: 3078 3078
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 3157 3276
Size: 54 1425 50 275 18 420 907 119
Cuts at: 3276 3769 4658 6287 8
Size: 493 889 1629 43
Fragments arranged by size:
1629 1425 907 889 493 420 275 119
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2256 2829 2841 3137 3317 4252 1353
Size: 774 129 573 12 296 180 935 3423
Fragments arranged by size:
3423 935 774 573 296 180 129 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4909 5283 5429 387
Size: 1064 138 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3409 3409
Size: 6322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2621 2767 3043 3590 4385 4982
Size: 393 737 252 146 276 547 795 597
Cuts at: 4982 5769 6037 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 737 597 547 393 276
268 252 146
EcoNI CCTnn'n_nnAGG
Cuts at: 3193 3515 3193
Size: 322 6000
EcoO109I rG'GnC_Cy
Cuts at: 2026 2368 2401 2933 3024 3186 2026
Size: 342 33 532 91 162 5162
Fragments arranged by size:
5162 532 342 162 91 33
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
EcoRV GAT'ATC
Cuts at: 2623 2623
Size: 6322
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 2237 5469 1678
Size: 559 3232 2531
Fragments arranged by size:
3232 2531 559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 3041 3073 3410
Size: 32 135 32 635 32 802 32 337
Cuts at: 3410 3442 3509 3541 4963 4995 5037 5069 1373
Size: 32 67 32 1422 32 42 32 2626
Fragments arranged by size:
2626 1422 802 635 337 135 67 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2233 2233
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5436 5620 1305
Size: 4131 184 2007
Fragments arranged by size:
4131 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4500
Fragments arranged by size:
4500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3459 4280 4639 4798 518
Size: 648 2293 821 359 159 2042
Fragments arranged by size:
2293 2042 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2355 2454 3738 4415 5563 5808 1606
Size: 598 151 99 1284 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 677 598 245 151 99
NaeI GCC'GGC
Cuts at: 2100 2194 2100
Size: 94 6228
NarI GG'CG_CC
Cuts at: 2234 2234
Size: 6322
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4831
Fragments arranged by size:
4831 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 2098 2192 2098
Size: 94 6228
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2969 3680 5225 1132
Size: 97 1740 711 1545 2229
Fragments arranged by size:
2229 1740 1545 711 97
PasI CC'CwG_GG
Cuts at: 1348 2690 1348
Size: 1342 4980
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2207 2961 1108
Size: 1099 754 4469
Fragments arranged by size:
4469 1099 754
PfoI T'CCnGG_A
Cuts at: 2744 3489 2744
Size: 745 5577
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 2401
Size: 6322
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2026 2933 2026
Size: 907 5415
PstI C_TGCA'G
Cuts at: 830 2274 3197 3674 830
Size: 1444 923 477 3478
Fragments arranged by size:
3478 1444 923 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SacI G_AGCT'C
Cuts at: 3411 3411
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 3317 2127
Size: 1190 5132
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SexAI A'CCwGG_T
Cuts at: 3168 3168
Size: 6322
SfcI C'TryA_G
Cuts at: 826 1045 2270 2442 3193 3670 4805 5486 5677
Size: 219 1225 172 751 477 1135 681 191
Cuts at: 5677 826
Size: 1471
Fragments arranged by size:
1471 1225 1135 751 681 477 219 191
172
SfiI GGCCn_nnn'nGGCC
Cuts at: 2090 2090
Size: 6322
SfoI GGC'GCC
Cuts at: 2235 2235
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 2031 2938 3649 1872
Size: 159 907 711 4545
Fragments arranged by size:
4545 907 711 159
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 3353 3500 3560 4426 5327
Size: 197 926 619 791 147 60 866 901
Cuts at: 5327 5589 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 866 791 619 262 197
147 60
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 2969 3680 1132
Size: 1837 711 3774
Fragments arranged by size:
3774 1837 711
SrfI GCCC'GGGC
Cuts at: 2031 2031
Size: 6322
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2325 1517
Size: 808 5514
StyI C'CwwG_G
Cuts at: 513 1065 2004 2508 2520 3761 513
Size: 552 939 504 12 1241 3074
Fragments arranged by size:
3074 1241 939 552 504 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2986 3471 4349 4366 4519 4704 5149
Size: 1255 1406 485 878 17 153 185 445
Cuts at: 5149 325
Size: 1498
Fragments arranged by size:
1498 1406 1255 878 485 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6282 6314 6282
Size: 32 6290
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 2029 2936 3647 1870
Size: 159 907 711 4545
Fragments arranged by size:
4545 907 711 159
XmnI GAAnn'nnTTC
Cuts at: 4451 4451
Size: 6322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SanDI SwaI XcmI