Restriction map for
pFN21AB7185
(Circular) (Six-base) MAPSORT of: pFN21AB7185.seq Check: 7574 from: 1 to: 6037
pFN21AB7185 6037 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2330 2330
Size: 6037
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2761 3380 1937
Size: 824 619 4594
Fragments arranged by size:
4594 824 619
Acc65I G'GTAC_C
Cuts at: 2127 3358 2127
Size: 1231 4806
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3033 4100 5484 5752 1632
Size: 1401 1067 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1067 268
AfeI AGC'GCT
Cuts at: 2642 2866 2642
Size: 224 5813
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 2710 1166
Size: 1544 4493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AlwNI CAG_nnn'CTG
Cuts at: 3780 5352 3780
Size: 1572 4465
ApaI G_GGCC'C
Cuts at: 2512 2512
Size: 6037
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 2117 2655 3113 3125 3346 3572 3724 5833
Size: 624 538 458 12 221 226 152 2109
Cuts at: 5833 1493
Size: 1697
Fragments arranged by size:
2109 1697 624 538 458 226 221 152
12
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 3362 1870
Size: 73 886 533 4545
Fragments arranged by size:
4545 886 533 73
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2127 2704 3358 4813
Size: 325 217 627 225 115 577 654 1455
Cuts at: 4813 618
Size: 1842
Fragments arranged by size:
1842 1455 654 627 577 325 225 217
115
BanII G_rGCy'C
Cuts at: 1391 2085 2512 3356 1391
Size: 694 427 844 4072
Fragments arranged by size:
4072 844 694 427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 928
Size: 971 1122 3944
Fragments arranged by size:
3944 1122 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2726 2760 4228 4262 1903
Size: 34 789 34 1468 34 3678
Fragments arranged by size:
3678 1468 789 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2833 4648 136
Size: 107 122 71 631 605 1161 1815 1525
Fragments arranged by size:
1815 1525 1161 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 2602 3454 2602
Size: 852 5185
Bme1580I G_kGCm'C
Cuts at: 1790 2512 4098 5254 1790
Size: 722 1586 1156 2573
Fragments arranged by size:
2573 1586 1156 722
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3343 3768 4726
Size: 416 53 83 399 24 1926 425 958
Cuts at: 4726 442
Size: 1753
Fragments arranged by size:
1926 1753 958 425 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4697 1239
Size: 3458 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2061 4162 5027 5325 2061
Size: 2101 865 298 2773
Fragments arranged by size:
2773 2101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 4700 882
Size: 617 852 2349 2219
Fragments arranged by size:
2349 2219 852 617
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3844 4226 4861 275
Size: 53 83 186 936 2311 382 635 1451
Fragments arranged by size:
2311 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6037
BseYI C'CCAG_C
Cuts at: 2643 3301 5240 5736 2643
Size: 658 1939 496 2944
Fragments arranged by size:
2944 1939 658 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2602 1163
Size: 573 396 470 4598
Fragments arranged by size:
4598 573 470 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4248 5276 1540
Size: 275 186 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2310 2498 3282 4098 4183 5254 2310
Size: 188 784 816 85 1071 3093
Fragments arranged by size:
3093 1071 816 784 188 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2310 2498 2512 3282 3356 4098
Size: 399 295 225 188 14 770 74 742
Cuts at: 4098 4183 5254 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 399 295 225 188
85 74 14
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 3926 5656 3926
Size: 1730 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2330 3392 844
Size: 1310 176 1062 3489
Fragments arranged by size:
3489 1310 1062 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4532 4706 65
Size: 1183 3284 174 1396
Fragments arranged by size:
3284 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4681 4868 1700
Size: 110 2871 187 2869
Fragments arranged by size:
2871 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 2309 4097 5109 2309
Size: 1788 1012 3237
Fragments arranged by size:
3237 1788 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3277 794
Size: 2483 3554
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 2266 1184
Size: 1082 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2575
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2766 2889 3367 3734 4119 4136 5588 5674
Size: 794 123 478 367 385 17 1452 86
Cuts at: 5674 5686 6032 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 794 478 385 367 346 123
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2562 2562
Size: 6037
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3152 487
Size: 683 56 1926 3372
Fragments arranged by size:
3372 1926 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 3553 4347 4367 2244
Size: 1309 794 20 3914
Fragments arranged by size:
3914 1309 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 2091 3130 3341 2091
Size: 1039 211 4787
Fragments arranged by size:
4787 1039 211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3484 4373 6002
Size: 54 1425 50 275 18 1654 889 1629
Cuts at: 6002 8
Size: 43
Fragments arranged by size:
1654 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3967 1353
Size: 2614 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3033 4100 4697 5484 5752 1239
Size: 393 1401 1067 597 787 268 1524
Fragments arranged by size:
1524 1401 1067 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2170 2170
Size: 6037
EcoRI G'AATT_C
Cuts at: 2117 3346 3724 2117
Size: 1229 378 4430
Fragments arranged by size:
4430 1229 378
EcoRV GAT'ATC
Cuts at: 2350 2350
Size: 6037
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 2644 2868 5184 1678
Size: 966 224 2316 2531
Fragments arranged by size:
2531 2316 966 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2383 2415 3239 3271 4678
Size: 32 135 32 811 32 824 32 1407
Cuts at: 4678 4710 4752 4784 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1407 824 811 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2762 3381 3627 669
Size: 988 281 824 619 246 3079
Fragments arranged by size:
3079 988 824 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 2131 3362 2131
Size: 1231 4806
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5151 5335 1305
Size: 3846 184 2007
Fragments arranged by size:
3846 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4215
Fragments arranged by size:
4215 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2552 2573 2710 3995 4354 4513
Size: 648 1026 360 21 137 1285 359 159
Cuts at: 4513 518
Size: 2042
Fragments arranged by size:
2042 1285 1026 648 360 359 159 137
21
MspA1I CmG'CkG
Cuts at: 1606 3453 4130 5278 5523 1606
Size: 1847 677 1148 245 2120
Fragments arranged by size:
2120 1847 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5485
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 3395 4940 1132
Size: 97 2166 1545 2229
Fragments arranged by size:
2229 2166 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6037
PfoI T'CCnGG_A
Cuts at: 2855 2855
Size: 6037
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 5619 5651 4106
Size: 32 1481 32 4492
Fragments arranged by size:
4492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2170
Size: 6037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 2552 3096 3607 2552
Size: 544 511 4982
Fragments arranged by size:
4982 544 511
PspOMI G'GGCC_C
Cuts at: 2508 2508
Size: 6037
PspXI vC'TCGA_Gb
Cuts at: 2829 2829
Size: 6037
PstI C_TGCA'G
Cuts at: 830 2814 3389 830
Size: 1984 575 3478
Fragments arranged by size:
3478 1984 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
SalI G'TCGA_C
Cuts at: 1936 2760 3379 1936
Size: 824 619 4594
Fragments arranged by size:
4594 824 619
SbfI CC_TGCA'GG
Cuts at: 3389 3389
Size: 6037
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SexAI A'CCwGG_T
Cuts at: 2360 2360
Size: 6037
SfcI C'TryA_G
Cuts at: 826 1045 2402 2784 2810 3385 4520 5201 5392
Size: 219 1357 382 26 575 1135 681 191
Cuts at: 5392 826
Size: 1471
Fragments arranged by size:
1471 1357 1135 681 575 382 219 191
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 3364 1872
Size: 1492 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2076 2829 4141 5042 5304 820
Size: 197 926 133 753 1312 901 262 1553
Fragments arranged by size:
1553 1312 926 901 753 262 197 133
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 2742 3961 5
Size: 47 2690 1219 2081
Fragments arranged by size:
2690 2081 1219 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6037
StyI C'CwwG_G
Cuts at: 513 1065 2451 3154 3476 513
Size: 552 1386 703 322 3074
Fragments arranged by size:
3074 1386 703 552 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 4081 4234 4419 4864 325
Size: 1255 2484 17 153 185 445 1498
Fragments arranged by size:
2484 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4283 5995 96
Size: 274 80 33 51 1443 2306 1712 138
Fragments arranged by size:
2306 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2311 2343 5997 6029 2311
Size: 32 3654 32 2319
Fragments arranged by size:
3654 2319 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2410 1316
Size: 1094 4943
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 3330 2341
Size: 989 5048
XhoI C'TCGA_G
Cuts at: 1943 2829 1943
Size: 886 5151
XmaI C'CCGG_G
Cuts at: 1870 3362 1870
Size: 1492 4545
XmnI GAAnn'nnTTC
Cuts at: 4166 4166
Size: 6037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI