Restriction map for
pFN21AB7180
(Circular) (Six-base) MAPSORT of: pFN21AB7180.seq Check: 5657 from: 1 to: 5965
pFN21AB7180 5965 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3230 3230
Size: 5965
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2664 4028 5412 5680 1632
Size: 1032 1364 1384 268 1917
Fragments arranged by size:
1917 1384 1364 1032 268
AfeI AGC'GCT
Cuts at: 2801 2801
Size: 5965
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 2264 4864 5841 1203
Size: 1061 2600 977 1327
Fragments arranged by size:
2600 1327 1061 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5965
AlwNI CAG_nnn'CTG
Cuts at: 2199 2325 3708 5280 2199
Size: 126 1383 1572 2884
Fragments arranged by size:
2884 1572 1383 126
ApaI G_GGCC'C
Cuts at: 2231 2509 3043 2231
Size: 278 534 5153
Fragments arranged by size:
5153 534 278
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 3274 3500 3652 5761 1493
Size: 1781 226 152 2109 1697
Fragments arranged by size:
2109 1781 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 2230 3290 1870
Size: 73 276 11 1060 4545
Fragments arranged by size:
4545 1060 276 73 11
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2372 3049 3178 3286 4741
Size: 325 217 627 585 677 129 108 1455
Cuts at: 4741 618
Size: 1842
Fragments arranged by size:
1842 1455 677 627 585 325 217 129
108
BanII G_rGCy'C
Cuts at: 1391 2231 2285 2509 3043 3084 3284 1391
Size: 840 54 224 534 41 200 4072
Fragments arranged by size:
4072 840 534 224 200 54 41
BbeI G_GCGC'C
Cuts at: 3182 3182
Size: 5965
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2588 928
Size: 971 689 4305
Fragments arranged by size:
4305 971 689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3215 3249 4156 4190 1903
Size: 34 1278 34 907 34 3678
Fragments arranged by size:
3678 1278 907 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3008 3027 3047
Size: 107 122 71 631 605 1336 19 20
Cuts at: 3047 3176 4576 136
Size: 129 1400 1525
Fragments arranged by size:
1525 1400 1336 631 605 129 122 107
71 20 19
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 2496 3382 2496
Size: 886 5079
Bme1580I G_kGCm'C
Cuts at: 1790 2231 2509 2993 3043 4026 5182 1790
Size: 441 278 484 50 983 1156 2573
Fragments arranged by size:
2573 1156 983 484 441 278 50
BmgBI CAC'GTC
Cuts at: 1318 2265 1318
Size: 947 5018
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3124 3696 4654
Size: 416 53 83 399 24 1707 572 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1707 958 572 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 4625 1239
Size: 1031 2355 2579
Fragments arranged by size:
2579 2355 1031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 4955 5253 4090
Size: 865 298 4802
Fragments arranged by size:
4802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4628 882
Size: 617 3129 2219
Fragments arranged by size:
3129 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2189 2521 2774 2880
Size: 53 83 186 936 656 332 253 106
Cuts at: 2880 3179 3772 4154 4789 275
Size: 299 593 382 635 1451
Fragments arranged by size:
1451 936 656 635 593 382 332 299
253 186 106 83 53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2900 1178
Size: 30 165 30 1467 30 4243
Fragments arranged by size:
4243 1467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2939 1186
Size: 1753 4212
BseYI C'CCAG_C
Cuts at: 2224 2349 2815 2824 2859 3067 5168 5664 2224
Size: 125 466 9 35 208 2101 496 2525
Fragments arranged by size:
2525 2101 496 466 208 125 35 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3202 1163
Size: 573 1466 3926
Fragments arranged by size:
3926 1466 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2370 2743 2851 2952 3031 3148
Size: 275 186 369 373 108 101 79 117
Cuts at: 3148 3415 4176 5204 1540
Size: 267 761 1028 2301
Fragments arranged by size:
2301 1028 761 373 369 275 267 186
117 108 101 79
BsiHKAI G_wGCw'C
Cuts at: 2319 3238 4026 4111 5182 2319
Size: 919 788 85 1071 3102
Fragments arranged by size:
3102 1071 919 788 85
BsmI GAATG_Cn'
Cuts at: 3096 3476 3569 3096
Size: 380 93 5492
Fragments arranged by size:
5492 380 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2512 2584 2512
Size: 72 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2285 2319 2509 2993 3043 3084
Size: 399 441 54 34 190 484 50 41
Cuts at: 3084 3238 3284 4026 4111 5182 1391
Size: 154 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 441 399 190 154
85 54 50 46 41 34
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2568 3168 3230 3320 844
Size: 1724 600 62 90 3489
Fragments arranged by size:
3489 1724 600 90 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3121 3150 3241 3852 1394
Size: 429 1298 29 91 611 3507
Fragments arranged by size:
3507 1298 611 429 91 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 2369 2846 4609 4796 1700
Size: 110 552 7 477 1763 187 2869
Fragments arranged by size:
2869 1763 552 477 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 2150 1925
Size: 225 5740
BssSI C'ACGA_G
Cuts at: 3231 4025 5037 3231
Size: 794 1012 4159
Fragments arranged by size:
4159 1012 794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2976 794
Size: 2182 3783
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstXI CCAn_nnnn'nTGG
Cuts at: 3067 3182 3067
Size: 115 5850
BstYI r'GATC_y
Cuts at: 1972 2195 3295 3662 4047 4064 5516 5602 5614
Size: 223 1100 367 385 17 1452 86 12
Cuts at: 5614 5960 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1100 385 367 346 223 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2432 2432
Size: 5965
BtgI C'CryG_G
Cuts at: 513 1065 2208 2544 3012 3022 3089 513
Size: 552 1143 336 468 10 67 3389
Fragments arranged by size:
3389 1143 552 468 336 67 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 2946 3481 4275 4295 2854
Size: 92 535 794 20 4524
Fragments arranged by size:
4524 794 535 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2848 2949 3028
Size: 54 1425 50 275 18 1018 101 79
Cuts at: 3028 3145 3412 4301 5930 8
Size: 117 267 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 275 267 117 101
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 2949 3028 3145 3412 1537
Size: 275 1036 101 79 117 267 4090
Fragments arranged by size:
4090 1036 275 267 117 101 79
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2905 3895 1353
Size: 686 866 990 3423
Fragments arranged by size:
3423 990 866 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2086 2349 2725 4552 4926 5072
Size: 1064 138 497 263 376 1827 374 146
Cuts at: 5072 387
Size: 1280
Fragments arranged by size:
1827 1280 1064 497 376 374 263 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2664 4028 4625 5412 5680 1239
Size: 393 638 394 1364 597 787 268 1524
Fragments arranged by size:
1524 1364 787 638 597 394 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2293 2505 3040 2293
Size: 212 535 5218
Fragments arranged by size:
5218 535 212
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FseI GG_CCGG'CC
Cuts at: 2850 2850
Size: 5965
FspI TGC'GCA
Cuts at: 2832 4471 2832
Size: 1639 4326
HaeII r_GCGC'y
Cuts at: 1678 2803 3182 5112 1678
Size: 1125 379 1930 2531
Fragments arranged by size:
2531 1930 1125 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2040 2051 2072 2254
Size: 32 135 32 447 21 11 21 182
Cuts at: 2254 2286 2586 2618 2870 2902 3134 3166 4606
Size: 32 300 32 252 32 232 32 1440
Cuts at: 4606 4638 4680 4712 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1440 447 300 252 232 182 135
42 32 32 32 32 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KasI G'GCGC_C
Cuts at: 3178 3178
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2080 5079 5263 1305
Size: 775 2999 184 2007
Fragments arranged by size:
2999 2007 775 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 3923 4282 4441 518
Size: 648 1935 822 359 159 2042
Fragments arranged by size:
2042 1935 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2349 2819 3024 3091 3211 3257 3381
Size: 651 92 470 205 67 120 46 124
Cuts at: 3381 4058 5206 5451 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 470 245 205 124
120 92 67 46
NaeI GCC'GGC
Cuts at: 2364 2848 2364
Size: 484 5481
NarI GG'CG_CC
Cuts at: 3179 3179
Size: 5965
NcoI C'CATG_G
Cuts at: 513 1065 2208 2544 513
Size: 552 1143 336 3934
Fragments arranged by size:
3934 1143 552 336
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NgoMIV G'CCGG_C
Cuts at: 2362 2846 2362
Size: 484 5481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3028 3412 3028
Size: 384 5581
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2705 3190 1348
Size: 1357 485 4123
Fragments arranged by size:
4123 1357 485
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2199 1108
Size: 902 189 4874
Fragments arranged by size:
4874 902 189
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2293
Size: 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspOMI G'GGCC_C
Cuts at: 2227 2505 3039 2227
Size: 278 534 5153
Fragments arranged by size:
5153 534 278
PstI C_TGCA'G
Cuts at: 830 2894 3317 830
Size: 2064 423 3478
Fragments arranged by size:
3478 2064 423
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2257 2819 2257
Size: 562 5403
SacII CC_GC'GG
Cuts at: 3025 3092 3025
Size: 67 5898
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2890 3313 4448 5129 5320 826
Size: 219 1845 423 1135 681 191 1471
Fragments arranged by size:
1845 1471 1135 681 423 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3008 3027 3008
Size: 19 5946
SfoI GGC'GCC
Cuts at: 3180 3180
Size: 5965
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 2362 1700
Size: 662 5303
SmaI CCC'GGG
Cuts at: 1872 2232 3292 1872
Size: 360 1060 4545
Fragments arranged by size:
4545 1060 360
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 4970 5232 820
Size: 197 926 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2208 2544 3404 513
Size: 552 1143 336 860 3074
Fragments arranged by size:
3074 1143 860 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2816 3992 4009 4162 4347 4792 325
Size: 1255 1236 1176 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1236 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 5925 5957 2380
Size: 32 3513 32 2388
Fragments arranged by size:
3513 2388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2551 2551
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 2230 3290 1870
Size: 360 1060 4545
Fragments arranged by size:
4545 1060 360
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SanDI SapI SexAI SrfI SwaI