Restriction map for
pFN21AB7178
(Circular) (Six-base) MAPSORT of: pFN21AB7178.seq Check: 422 from: 1 to: 5983
pFN21AB7178 5983 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2314 3326 1937
Size: 377 1012 4594
Fragments arranged by size:
4594 1012 377
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2903 4046 5430 5698 1632
Size: 1271 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1271 1143 268
AflII C'TTAA_G
Cuts at: 820 1017 2416 820
Size: 197 1399 4387
Fragments arranged by size:
4387 1399 197
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2894 1166
Size: 1728 4255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2565 1184
Size: 1381 4602
AlwNI CAG_nnn'CTG
Cuts at: 2935 3726 5298 2935
Size: 791 1572 3620
Fragments arranged by size:
3620 1572 791
ApaI G_GGCC'C
Cuts at: 2662 2662
Size: 5983
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2062 3292 3518 3670 5779 1493
Size: 569 1230 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 569 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 3308 1870
Size: 73 802 563 4545
Fragments arranged by size:
4545 802 563 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2828 3304 4759 618
Size: 325 217 627 225 816 476 1455 1842
Fragments arranged by size:
1842 1455 816 627 476 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2662 3302 1391
Size: 1271 640 4072
Fragments arranged by size:
4072 1271 640
BbeI G_GCGC'C
Cuts at: 2832 2832
Size: 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 2267 2516 928
Size: 971 315 53 249 4395
Fragments arranged by size:
4395 971 315 249 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2723 2757 4174 4208 1903
Size: 34 786 34 1417 34 3678
Fragments arranged by size:
3678 1417 786 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 4594 136
Size: 107 122 71 631 605 985 1937 1525
Fragments arranged by size:
1937 1525 985 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2919 5978 2919
Size: 3059 2924
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2662 4044 5200 1790
Size: 872 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 872
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2665 3112 3714
Size: 416 53 83 399 24 1248 447 602
Cuts at: 3714 4672 442
Size: 958 1753
Fragments arranged by size:
1753 1248 958 602 447 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 4643 1239
Size: 819 2585 2579
Fragments arranged by size:
2585 2579 819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 4108 4973 5271 2090
Size: 2018 865 298 2802
Fragments arranged by size:
2802 2018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2624 4646 882
Size: 617 1125 2022 2219
Fragments arranged by size:
2219 2022 1125 617
BsaAI yAC'GTr
Cuts at: 493 1204 2341 2587 2800 2878 3774 493
Size: 711 1137 246 213 78 896 2702
Fragments arranged by size:
2702 1137 896 711 246 213 78
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2534 2829 3790 4172
Size: 53 83 186 936 1001 295 961 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 1001 961 936 635 382 295 186
83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2666 2696 1178
Size: 30 165 30 1263 30 4465
Fragments arranged by size:
4465 1263 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 4626
BseYI C'CCAG_C
Cuts at: 2910 5186 5682 2910
Size: 2276 496 3211
Fragments arranged by size:
3211 2276 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2697 2805 4044 4129 5200 2697
Size: 108 1239 85 1071 3480
Fragments arranged by size:
3480 1239 1071 108 85
BsmI GAATG_Cn'
Cuts at: 2623 3494 3587 2623
Size: 871 93 5019
Fragments arranged by size:
5019 871 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2525 2525
Size: 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 2697 2805 3302 4044 4129 5200
Size: 399 872 35 108 497 742 85 1071
Cuts at: 5200 1391
Size: 2174
Fragments arranged by size:
2174 1071 872 742 497 399 108 85
35
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 2444 96
Size: 2348 3635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 794
Size: 2021 3962
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 2887 1184
Size: 1703 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2845 2479
Size: 366 5617
BstYI r'GATC_y
Cuts at: 1972 2376 2919 3313 3680 4065 4082 5534 5620
Size: 404 543 394 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 543 404 394 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2944 513
Size: 552 939 940 3552
Fragments arranged by size:
3552 940 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 487
Size: 683 56 1403 3841
Fragments arranged by size:
3841 1403 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 3499 4293 4313 2016
Size: 1483 794 20 3686
Fragments arranged by size:
3686 1483 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 2761 3287 2761
Size: 526 5457
DraIII CAC_nnn'GTG
Cuts at: 3063 3063
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 2355 2640
Size: 54 1425 50 275 18 430 95 285
Cuts at: 2640 3430 4319 5948 8
Size: 790 889 1629 43
Fragments arranged by size:
1629 1425 889 790 430 285 275 95
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 3064 3913 1353
Size: 987 724 849 3423
Fragments arranged by size:
3423 987 849 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2903 4046 4643 5430 5698 1239
Size: 393 426 845 1143 597 787 268 1524
Fragments arranged by size:
1524 1143 845 787 597 426 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2774 2774
Size: 5983
EcoO109I rG'GnC_Cy
Cuts at: 2659 2659
Size: 5983
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
EcoRV GAT'ATC
Cuts at: 2851 2851
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2078 2832 5130 1678
Size: 400 754 2298 2531
Fragments arranged by size:
2531 2298 754 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 4624 4656 4698
Size: 32 135 32 1175 32 1845 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 1175 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 2828 2828
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 2404 2477 3015 3582 2404
Size: 73 538 567 4805
Fragments arranged by size:
4805 567 538 73
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 2357 2642 10
Size: 54 1425 343 430 95 285 3351
Fragments arranged by size:
3351 1425 430 343 285 95 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 2894 3941 4300 4459 518
Size: 648 1644 84 1047 359 159 2042
Fragments arranged by size:
2042 1644 1047 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 3399 4076 5224 5469 1606
Size: 1793 677 1148 245 2120
Fragments arranged by size:
2120 1793 1148 677 245
NarI GG'CG_CC
Cuts at: 2829 2829
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 2004 2944 513
Size: 552 939 940 3552
Fragments arranged by size:
3552 940 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2716 3341 4886 1132
Size: 97 1487 625 1545 2229
Fragments arranged by size:
2229 1545 1487 625 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2341 2800 2341
Size: 459 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 2184 3553 2184
Size: 1369 4614
PspOMI G'GGCC_C
Cuts at: 2658 2658
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 3064 3064
Size: 5983
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 3251 4231 5943 3251
Size: 980 1712 3291
Fragments arranged by size:
3291 1712 980
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5983
SfcI C'TryA_G
Cuts at: 826 1045 2311 3331 4466 5147 5338 826
Size: 219 1266 1020 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 1020 681 219 191
SfoI GGC'GCC
Cuts at: 2830 2830
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2416 4087 4988 5250 820
Size: 197 926 126 347 1671 901 262 1553
Fragments arranged by size:
1671 1553 926 901 347 262 197 126
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 2716 3341 1132
Size: 1584 625 3774
Fragments arranged by size:
3774 1584 625
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2238 2993 1517
Size: 721 755 4507
Fragments arranged by size:
4507 755 721
StyI C'CwwG_G
Cuts at: 513 1065 2004 2944 3146 3422 513
Size: 552 939 940 202 276 3074
Fragments arranged by size:
3074 940 939 552 276 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2444 2682 3249
Size: 274 80 33 51 1443 467 238 567
Cuts at: 3249 4229 5941 96
Size: 980 1712 138
Fragments arranged by size:
1712 1443 980 567 467 274 238 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5943 5975 5943
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2274 4112 2274
Size: 1838 4145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI