Restriction map for
pFN21AB7176
(Circular) (Six-base) MAPSORT of: pFN21AB7176.seq Check: 6467 from: 1 to: 5800
pFN21AB7176 5800 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 3143 1937
Size: 397 809 4594
Fragments arranged by size:
4594 809 397
Acc65I G'GTAC_C
Cuts at: 2085 3121 2085
Size: 1036 4764
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2197 2488 3863 5247 5515 1632
Size: 554 11 291 1375 1384 268 1917
Fragments arranged by size:
1917 1384 1375 554 291 268 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2684 1166
Size: 1518 4282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2582 2786 3543 5115 2582
Size: 204 757 1572 3267
Fragments arranged by size:
3267 1572 757 204
ApaI G_GGCC'C
Cuts at: 2013 2037 2441 2724 2964 2013
Size: 24 404 283 240 4849
Fragments arranged by size:
4849 404 283 240 24
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2955 3048 3125 1870
Size: 73 1012 93 77 4545
Fragments arranged by size:
4545 1012 93 77 73
AvrII C'CTAG_G
Cuts at: 2744 2744
Size: 5800
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2284 2542 2614 2774
Size: 325 217 627 298 199 258 72 160
Cuts at: 2774 2884 3004 3121 4576 618
Size: 110 120 117 1455 1842
Fragments arranged by size:
1842 1455 627 325 298 258 217 199
160 120 117 110 72
BanII G_rGCy'C
Cuts at: 1391 2013 2037 2441 2724 2964 3119 1391
Size: 622 24 404 283 240 155 4072
Fragments arranged by size:
4072 622 404 283 240 155 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 928
Size: 971 327 4502
Fragments arranged by size:
4502 971 327
BbvCI CC'TCA_GC
Cuts at: 2355 2355
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2467 2653 2968
Size: 107 122 71 631 605 795 186 315
Cuts at: 2968 4411 136
Size: 1443 1525
Fragments arranged by size:
1525 1443 795 631 605 315 186 122
107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2037 2289 2441 2545 2619 2724 2777
Size: 223 24 252 152 104 74 105 53
Cuts at: 2777 2964 3861 5017 1790
Size: 187 897 1156 2573
Fragments arranged by size:
2573 1156 897 252 223 187 152 105
104 74 53 24
BmgBI CAC'GTC
Cuts at: 1318 2338 1318
Size: 1020 4780
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2469 2487 2570
Size: 416 53 83 399 24 1052 18 83
Cuts at: 2570 2902 2944 3531 4489 442
Size: 332 42 587 958 1753
Fragments arranged by size:
1753 1052 958 587 416 399 332 83
83 53 42 24 18
BmtI G_CTAG'C
Cuts at: 1056 2561 1056
Size: 1505 4295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
Bpu10I CC'TnA_GC
Cuts at: 2355 2355
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2446 3591 493
Size: 711 1242 1145 2702
Fragments arranged by size:
2702 1242 1145 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2988 3607 3989 4624
Size: 53 83 186 936 1455 619 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1455 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 2801 2831 1178
Size: 30 165 30 843 30 525 30 4147
Fragments arranged by size:
4147 843 525 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2621 1186
Size: 900 535 4365
Fragments arranged by size:
4365 900 535
BseYI C'CCAG_C
Cuts at: 2058 2350 5003 5499 2058
Size: 292 2653 496 2359
Fragments arranged by size:
2653 2359 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 2723 3112 1163
Size: 573 699 288 389 3851
Fragments arranged by size:
3851 699 573 389 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2334 2458 2471 3250 4011 5039
Size: 275 186 333 124 13 779 761 1028
Cuts at: 5039 1540
Size: 2301
Fragments arranged by size:
2301 1028 779 761 333 275 186 124
13
BsiHKAI G_wGCw'C
Cuts at: 3861 3946 5017 3861
Size: 85 1071 4644
Fragments arranged by size:
4644 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2037 2289 2441 2545 2619 2724
Size: 399 223 24 252 152 104 74 105
Cuts at: 2724 2777 2964 3119 3861 3946 5017 1391
Size: 53 187 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 252 223 187 155
152 105 104 85 74 53 24
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 3155 844
Size: 1253 1058 3489
Fragments arranged by size:
3489 1253 1058
BsrBI CCG'CTC
Cuts at: 1394 1823 2150 3687 1394
Size: 429 327 1537 3507
Fragments arranged by size:
3507 1537 429 327
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2388 2457 2466 4444 4631 1700
Size: 110 578 69 9 1978 187 2869
Fragments arranged by size:
2869 1978 578 187 110 69 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2680 3860 4872 2680
Size: 1180 1012 3608
Fragments arranged by size:
3608 1180 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2686 2434
Size: 252 5548
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DraIII CAC_nnn'GTG
Cuts at: 2495 2495
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3035 4807 809
Size: 507 1719 1772 1802
Fragments arranged by size:
1802 1772 1719 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2455 2459
Size: 54 1425 50 275 18 291 334 4
Cuts at: 2459 2468 2549 3247 4136 5765 8
Size: 9 81 698 889 1629 43
Fragments arranged by size:
1629 1425 889 698 334 291 275 81
54 50 43 18 9 4
EagI C'GGCC_G
Cuts at: 1537 1812 2455 2468 3247 1537
Size: 275 643 13 779 4090
Fragments arranged by size:
4090 779 643 275 13
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2641 2999 3730 1353
Size: 722 566 358 731 3423
Fragments arranged by size:
3423 731 722 566 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2197 2488 3863 4460 5247 5515
Size: 393 554 11 291 1375 597 787 268
Cuts at: 5515 1239
Size: 1524
Fragments arranged by size:
1524 1375 787 597 554 393 291 268
11
EcoNI CCTnn'n_nnAGG
Cuts at: 2212 3069 2212
Size: 857 4943
EcoO109I rG'GnC_Cy
Cuts at: 2009 2010 2033 2034 2293 2431 2438 2721 2748
Size: 1 23 1 259 138 7 283 27
Cuts at: 2748 2814 2960 2961 3052 2009
Size: 66 146 1 91 4757
Fragments arranged by size:
4757 283 259 146 138 91 66 27
23 7 1 1 1
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 5800
FseI GG_CCGG'CC
Cuts at: 2461 2461
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2363 2395 4947 1678
Size: 685 32 2552 2531
Fragments arranged by size:
2552 2531 685 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2170 2202 4441
Size: 32 135 32 461 32 105 32 2239
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2239 461 135 105 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 2919 3144 3390 669
Size: 988 281 397 584 225 246 3079
Fragments arranged by size:
3079 988 584 397 281 246 225
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 2089 3125 2089
Size: 1036 4764
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 4914 5098 1305
Size: 984 2625 184 2007
Fragments arranged by size:
2625 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 10
Size: 54 1425 343 291 3687
Fragments arranged by size:
3687 1425 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 3758 4117 4276 518
Size: 648 1518 1074 359 159 2042
Fragments arranged by size:
2042 1518 1074 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2354 2802 2833 3216 3893 5041 5286
Size: 413 335 448 31 383 677 1148 245
Cuts at: 5286 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 448 413 383 335 245
31
NaeI GCC'GGC
Cuts at: 2390 2459 2390
Size: 69 5731
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2388 2457 2388
Size: 69 5731
NheI G'CTAG_C
Cuts at: 1052 2557 1052
Size: 1505 4295
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2966 1348
Size: 1618 4182
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2431 3052 2293
Size: 138 621 5041
Fragments arranged by size:
5041 621 138
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2009 2033 2437 2720 2960 2009
Size: 24 404 283 240 4849
Fragments arranged by size:
4849 404 283 240 24
PstI C_TGCA'G
Cuts at: 830 2018 2373 2587 2772 3152 830
Size: 1188 355 214 185 380 3478
Fragments arranged by size:
3478 1188 380 355 214 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2354 2833 2354
Size: 479 5321
SalI G'TCGA_C
Cuts at: 1936 2333 3142 1936
Size: 397 809 4594
Fragments arranged by size:
4594 809 397
SbfI CC_TGCA'GG
Cuts at: 2587 3152 2587
Size: 565 5235
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2014 2369 2583 2768 3148 4283 4964
Size: 219 969 355 214 185 380 1135 681
Cuts at: 4964 5155 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 681 380 355 219 214
191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2467 2653 2467
Size: 186 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2647 1517
Size: 1130 4670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2289 2313 2427 2744 2880
Size: 552 939 129 156 24 114 317 136
Cuts at: 2880 2998 3239 513
Size: 118 241 3074
Fragments arranged by size:
3074 939 552 317 241 156 136 129
118 114 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2506 2908 3025 3827 3844 3997 4182
Size: 1255 926 402 117 802 17 153 185
Cuts at: 4182 4627 325
Size: 445 1498
Fragments arranged by size:
1498 1255 926 802 445 402 185 153
117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2767 2799 2801 2833 2887 2919 5760
Size: 32 492 32 2 32 54 32 2841
Cuts at: 5760 5792 2243
Size: 32 2251
Fragments arranged by size:
2841 2251 492 54 32 32 32 32
32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 2983 3136 2983
Size: 153 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2131
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI