Restriction map for
pFN21AB7173
(Circular) (Six-base) MAPSORT of: pFN21AB7173.seq Check: 4003 from: 1 to: 6295
pFN21AB7173 6295 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2278 2278
Size: 6295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2888 3413 4358 5742 6010 1632
Size: 605 651 525 945 1384 268 1917
Fragments arranged by size:
1917 1384 945 651 605 525 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 2017 5024 2017
Size: 3007 3288
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 6263
AlwNI CAG_nnn'CTG
Cuts at: 2245 2509 4038 5610 2245
Size: 264 1529 1572 2930
Fragments arranged by size:
2930 1572 1529 264
ApaI G_GGCC'C
Cuts at: 2360 2604 2360
Size: 244 6051
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2572 3153 3604 3830 3982 6091 1493
Size: 1079 581 451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 581 451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2489 2597 3620 1870
Size: 73 309 237 108 1023 4545
Fragments arranged by size:
4545 1023 309 237 108 73
BamHI G'GATC_C
Cuts at: 2520 3625 3992 2520
Size: 1105 367 4823
Fragments arranged by size:
4823 1105 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 2758 2837 3616 5071
Size: 325 217 627 911 60 79 779 1455
Cuts at: 5071 618
Size: 1842
Fragments arranged by size:
1842 1455 911 779 627 325 217 79
60
BanII G_rGCy'C
Cuts at: 1391 2264 2316 2360 2544 2604 3483 3614 1391
Size: 873 52 44 184 60 879 131 4072
Fragments arranged by size:
4072 879 873 184 131 60 52 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3423 928
Size: 971 1524 3800
Fragments arranged by size:
3800 1524 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2715 4187 5397 2715
Size: 1472 1210 3613
Fragments arranged by size:
3613 1472 1210
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 2094 2909 3239 3712 2094
Size: 815 330 473 4677
Fragments arranged by size:
4677 815 473 330
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2594 2604 2643 2979 4356 5512 1790
Size: 570 234 10 39 336 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 570 336 234 39 10
BmgBI CAC'GTC
Cuts at: 1318 2392 3174 1318
Size: 1074 782 4439
Fragments arranged by size:
4439 1074 782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2258 3135 4026
Size: 416 53 83 399 24 841 877 891
Cuts at: 4026 4984 442
Size: 958 1753
Fragments arranged by size:
1753 958 891 877 841 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2402 2431 2552 2773 2870 4955 1239
Size: 860 303 29 121 221 97 2085 2579
Fragments arranged by size:
2579 2085 860 303 221 121 97 29
Bpu10I CC'TnA_GC
Cuts at: 3112 3112
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2969 4420 5285 5583 2969
Size: 1451 865 298 3681
Fragments arranged by size:
3681 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3054 3504 4728 5126 5400 5547 5958 3054
Size: 450 1224 398 274 147 411 3391
Fragments arranged by size:
3391 1224 450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2393 2423 3534
Size: 30 165 30 708 30 252 30 1111
Cuts at: 3534 3564 1178
Size: 30 3909
Fragments arranged by size:
3909 1111 708 252 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2434 2610 1186
Size: 1248 176 4871
Fragments arranged by size:
4871 1248 176
BseYI C'CCAG_C
Cuts at: 2496 2502 2525 3187 3257 5498 5994 2496
Size: 6 23 662 70 2241 496 2797
Fragments arranged by size:
2797 2241 662 496 70 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 1163
Size: 573 1071 4651
Fragments arranged by size:
4651 1071 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 2568 4356 4441 5512 2316
Size: 252 1788 85 1071 3099
Fragments arranged by size:
3099 1788 1071 252 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2316 2360 2544 2568 2594 2604
Size: 399 474 52 44 184 24 26 10
Cuts at: 2604 2643 2979 3483 3614 4356 4441 5512 1391
Size: 39 336 504 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 474 399 336 184
131 85 52 44 39 26 24 10
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 2343 4184 5914 2343
Size: 1841 1730 2724
Fragments arranged by size:
2724 1841 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 2278 3650 844
Size: 1178 256 1372 3489
Fragments arranged by size:
3489 1372 1178 256
BsrBI CCG'CTC
Cuts at: 1394 1823 2480 4182 1394
Size: 429 657 1702 3507
Fragments arranged by size:
3507 1702 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 4790 4964 65
Size: 1183 1087 2455 174 1396
Fragments arranged by size:
2455 1396 1183 1087 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 5324
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2695 3148 2137
Size: 558 453 5284
Fragments arranged by size:
5284 558 453
BstYI r'GATC_y
Cuts at: 1972 2520 2956 3625 3992 4377 4394 5846 5932
Size: 548 436 669 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 669 548 436 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2516 2892 3485 2516
Size: 376 593 5326
Fragments arranged by size:
5326 593 376
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2373 3465 513
Size: 552 939 30 339 1092 3343
Fragments arranged by size:
3343 1092 939 552 339 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 3811 4605 4625 3223
Size: 588 794 20 4893
Fragments arranged by size:
4893 794 588 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 2833 3599 2833
Size: 766 5529
DraIII CAC_nnn'GTG
Cuts at: 3124 3124
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 3312 3573
Size: 54 1425 50 275 18 396 1086 261
Cuts at: 3573 3742 4631 6260 8
Size: 169 889 1629 43
Fragments arranged by size:
1629 1425 1086 889 396 275 261 169
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 3453 4225 1353
Size: 1121 979 772 3423
Fragments arranged by size:
3423 1121 979 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2314 2314
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2237 2402 2431 2552 2773 2870
Size: 393 467 138 165 29 121 221 97
Cuts at: 2870 2888 3413 4358 4955 5742 6010 1239
Size: 18 525 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 525 467 393 268
221 165 138 121 97 29 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 2471 2426
Size: 45 6250
EcoO109I rG'GnC_Cy
Cuts at: 2149 2356 2357 2434 2485 2601 2950 3184 2149
Size: 207 1 77 51 116 349 234 5260
Fragments arranged by size:
5260 349 234 207 116 77 51 1
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 3187 3219 4936
Size: 32 135 32 431 32 1152 32 1717
Cuts at: 4936 4968 5010 5042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1717 1152 431 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3575 10
Size: 54 1425 343 1743 2730
Fragments arranged by size:
2730 1743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2282 2288 2756 2930 3125 3290
Size: 648 873 243 6 468 174 195 165
Cuts at: 3290 4253 4612 4771 518
Size: 963 359 159 2042
Fragments arranged by size:
2042 963 873 648 468 359 243 195
174 165 159 6
MspA1I CmG'CkG
Cuts at: 1606 2098 3261 3711 4388 5536 5781 1606
Size: 492 1163 450 677 1148 245 2120
Fragments arranged by size:
2120 1163 1148 677 492 450 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2034 3465 513
Size: 552 939 30 1431 3343
Fragments arranged by size:
3343 1431 939 552 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2287 3550 3653 5198 1132
Size: 97 1058 1263 103 1545 2229
Fragments arranged by size:
2229 1545 1263 1058 103 97
PasI CC'CwG_GG
Cuts at: 1348 2353 2587 1348
Size: 1005 234 5056
Fragments arranged by size:
5056 1005 234
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2509 1108
Size: 1401 4894
PfoI T'CCnGG_A
Cuts at: 2508 3015 2508
Size: 507 5788
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 4364 4396 5877 5909 2111
Size: 32 2221 32 1481 32 2497
Fragments arranged by size:
2497 2221 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2485 2485
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2356 2600 2356
Size: 244 6051
PstI C_TGCA'G
Cuts at: 830 2019 3647 830
Size: 1189 1628 3478
Fragments arranged by size:
3478 1628 1189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2098 2098
Size: 6295
SacI G_AGCT'C
Cuts at: 2316 2316
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 2900 4543 6255 2900
Size: 1643 1712 2940
Fragments arranged by size:
2940 1712 1643
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 6295
SfcI C'TryA_G
Cuts at: 826 1045 2015 3643 4778 5459 5650 826
Size: 219 970 1628 1135 681 191 1471
Fragments arranged by size:
1628 1471 1135 970 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2984 4399 5300 5562 820
Size: 197 926 1041 1415 901 262 1553
Fragments arranged by size:
1553 1415 1041 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 2114 3375 1517
Size: 597 1261 4437
Fragments arranged by size:
4437 1261 597
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2061 3315 3451 3465 3734
Size: 552 939 30 27 1254 136 14 269
Cuts at: 3734 513
Size: 3074
Fragments arranged by size:
3074 1254 939 552 269 136 30 27
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2366 2855 4322 4339 4492 4677 5122
Size: 1255 786 489 1467 17 153 185 445
Cuts at: 5122 325
Size: 1498
Fragments arranged by size:
1498 1467 1255 786 489 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 4541 6253
Size: 274 80 33 51 1443 921 1643 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1712 1643 1443 921 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 3044 3076 3568 3600 6255 6287 2393
Size: 32 619 32 492 32 2655 32 2401
Fragments arranged by size:
2655 2401 619 492 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI