Restriction map for
pFN21AB7172
(Circular) (Six-base) MAPSORT of: pFN21AB7172.seq Check: 8502 from: 1 to: 6178
pFN21AB7172 6178 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3521 1937
Size: 883 701 4594
Fragments arranged by size:
4594 883 701
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2172 4241 5625 5893 1632
Size: 540 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 540 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 1184
Size: 936 5242
AlwNI CAG_nnn'CTG
Cuts at: 3921 5493 3921
Size: 1572 4606
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 2245 2750 2901 3487 3713 3865 5974 1493
Size: 752 505 151 586 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 586 505 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 1870
Size: 73 1560 4545
Fragments arranged by size:
4545 1560 73
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2945 3499 4954 618
Size: 325 217 627 1158 554 1455 1842
Fragments arranged by size:
1842 1455 1158 627 554 325 217
BanII G_rGCy'C
Cuts at: 1391 2337 3497 1391
Size: 946 1160 4072
Fragments arranged by size:
4072 1160 946
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3432 928
Size: 971 1533 3674
Fragments arranged by size:
3674 1533 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BfrBI ATG'CAT
Cuts at: 3174 3174
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 4789 136
Size: 107 122 71 631 605 1141 1976 1525
Fragments arranged by size:
1976 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6173 6173
Size: 6178
BlpI GC'TnA_GC
Cuts at: 2556 3595 2556
Size: 1039 5139
Bme1580I G_kGCm'C
Cuts at: 1790 4239 5395 1790
Size: 2449 1156 2573
Fragments arranged by size:
2573 2449 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 2329 2410
Size: 416 53 83 399 24 718 194 81
Cuts at: 2410 3909 4867 442
Size: 1499 958 1753
Fragments arranged by size:
1753 1499 958 718 416 399 194 83
81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2492 4838 1239
Size: 947 306 2346 2579
Fragments arranged by size:
2579 2346 947 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 4303 5168 5466 2599
Size: 1704 865 298 3311
Fragments arranged by size:
3311 1704 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2943 4841 882
Size: 617 1444 1898 2219
Fragments arranged by size:
2219 1898 1444 617
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 2217 6172 2217
Size: 3955 2223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2505 2662 4611 5009 5283 5430 5841 2505
Size: 157 1949 398 274 147 411 2842
Fragments arranged by size:
2842 1949 411 398 274 157 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2330 2672 2702
Size: 30 165 30 867 30 30 342 30
Cuts at: 2702 3437 3467 1178
Size: 735 30 3889
Fragments arranged by size:
3889 867 735 342 165 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 2425 2563 1186
Size: 1108 131 138 4801
Fragments arranged by size:
4801 1108 138 131
BseYI C'CCAG_C
Cuts at: 5381 5877 5381
Size: 496 5682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3295 1163
Size: 573 1559 4046
Fragments arranged by size:
4046 1559 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 4239 4324 5395 2337
Size: 1902 85 1071 3120
Fragments arranged by size:
3120 1902 1071 85
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
BsmBI CGTCTCn'nnnn_
Cuts at: 2457 2457
Size: 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 3497 4239 4324 5395 1391
Size: 399 547 1160 742 85 1071 2174
Fragments arranged by size:
2174 1160 1071 742 547 399 85
BspEI T'CCGG_A
Cuts at: 2505 5841 2505
Size: 3336 2842
BspHI T'CATG_A
Cuts at: 3301 4067 5797 3301
Size: 766 1730 3682
Fragments arranged by size:
3682 1730 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 844
Size: 2689 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2735 4065 1394
Size: 429 912 1330 3507
Fragments arranged by size:
3507 1330 912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 4673 4847 65
Size: 1183 1443 1982 174 1396
Fragments arranged by size:
1982 1443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 2390 4238 5250 2390
Size: 1848 1012 3318
Fragments arranged by size:
3318 1848 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6178
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 3139 1184
Size: 1955 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 2033 2975 3457 2033
Size: 942 482 4754
Fragments arranged by size:
4754 942 482
BstYI r'GATC_y
Cuts at: 1972 2874 3508 3875 4260 4277 5729 5815 5827
Size: 902 634 367 385 17 1452 86 12
Cuts at: 5827 6173 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 902 634 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3000 3036 513
Size: 552 939 996 36 3655
Fragments arranged by size:
3655 996 939 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2012 3145 487
Size: 683 56 786 1133 3520
Fragments arranged by size:
3520 1133 786 683 56
BtsI GCAGTG_nn'
Cuts at: 2486 3283 3694 4488 4508 2486
Size: 797 411 794 20 4156
Fragments arranged by size:
4156 797 794 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2604 3625
Size: 54 1425 50 275 18 360 414 1021
Cuts at: 3625 4514 6143 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1021 889 414 360 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2342 2439 2829 2996 3376 4108 1353
Size: 795 194 97 390 167 380 732 3423
Fragments arranged by size:
3423 795 732 390 380 194 167 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 6178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2186 2492 4241 4838 5625 5893
Size: 393 540 14 306 1749 597 787 268
Cuts at: 5893 1239
Size: 1524
Fragments arranged by size:
1749 1524 787 597 540 393 306 268
14
EcoRI G'AATT_C
Cuts at: 2901 3487 3865 2901
Size: 586 378 5214
Fragments arranged by size:
5214 586 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2996 2996
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 5325 1678
Size: 3647 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 2771 2803 3041
Size: 32 135 32 1095 32 72 32 238
Cuts at: 3041 3073 3386 3418 4819 4851 4893 4925 1373
Size: 32 313 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 1095 313 238 135 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 2089 3522 3768 669
Size: 988 281 118 33 1433 246 3079
Fragments arranged by size:
3079 1433 988 281 246 118 33
HindIII A'AGCT_T
Cuts at: 748 2558 2909 748
Size: 1810 351 4017
Fragments arranged by size:
4017 1810 351
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 5292 5476 1305
Size: 1302 2685 184 2007
Fragments arranged by size:
2685 2007 1302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2606 10
Size: 54 1425 343 774 3582
Fragments arranged by size:
3582 1425 774 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 3191 3263 3386 4136 4495 4654
Size: 648 1938 87 72 123 750 359 159
Cuts at: 4654 518
Size: 2042
Fragments arranged by size:
2042 1938 750 648 359 159 123 87
72
MspA1I CmG'CkG
Cuts at: 1606 3343 3594 4271 5419 5664 1606
Size: 1737 251 677 1148 245 2120
Fragments arranged by size:
2120 1737 1148 677 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4687
Fragments arranged by size:
4687 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NsiI A_TGCA'T
Cuts at: 3176 3176
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2273 2626 3174 3536 5081 1132
Size: 97 1044 353 548 362 1545 2229
Fragments arranged by size:
2229 1545 1044 548 362 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3084 1108
Size: 1976 4202
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2935 1613
Size: 1322 4856
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PstI C_TGCA'G
Cuts at: 830 2357 3530 830
Size: 1527 1173 3478
Fragments arranged by size:
3478 1527 1173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SapI GCTCTTCn'nnn_
Cuts at: 2342 2342
Size: 6178
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 2318 3239 4426 6138 2318
Size: 921 1187 1712 2358
Fragments arranged by size:
2358 1712 1187 921
SfcI C'TryA_G
Cuts at: 826 1045 2353 2817 3375 3526 4661 5342 5533
Size: 219 1308 464 558 151 1135 681 191
Cuts at: 5533 826
Size: 1471
Fragments arranged by size:
1471 1308 1135 681 558 464 219 191
151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2578 4282 5183 5445 820
Size: 197 926 635 1704 901 262 1553
Fragments arranged by size:
1704 1553 926 901 635 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6178
StyI C'CwwG_G
Cuts at: 513 1065 2004 2826 3144 3456 3617 513
Size: 552 939 822 318 312 161 3074
Fragments arranged by size:
3074 939 822 552 318 312 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2925 3146 4205 4222 4375 4560 5005
Size: 1255 1345 221 1059 17 153 185 445
Cuts at: 5005 325
Size: 1498
Fragments arranged by size:
1498 1345 1255 1059 445 221 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 3237 4424
Size: 274 80 33 51 1443 339 921 1187
Cuts at: 4424 6136 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1187 921 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2672 2704 6138 6170 2270
Size: 32 370 32 3434 32 2278
Fragments arranged by size:
3434 2278 370 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 1316
Size: 1467 4711
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2642 2642
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI