Restriction map for
pFN21AB7169
(Circular) (Six-base) MAPSORT of: pFN21AB7169.seq Check: 3919 from: 1 to: 6004
pFN21AB7169 6004 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 4067 5451 5719 1632
Size: 1079 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 1079 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2076 2734 3747 5319 2076
Size: 658 1013 1572 2761
Fragments arranged by size:
2761 1572 1013 658
ApaLI G'TGCA_C
Cuts at: 2294 4061 5217 2294
Size: 1767 1156 3081
Fragments arranged by size:
3081 1767 1156
ApoI r'AATT_y
Cuts at: 1493 3313 3539 3691 5800 1493
Size: 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2943 3035 3329 1870
Size: 73 1000 92 294 4545
Fragments arranged by size:
4545 1000 294 92 73
BamHI G'GATC_C
Cuts at: 2103 2532 2697 3334 3701 2103
Size: 429 165 637 367 4406
Fragments arranged by size:
4406 637 429 367 165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3323 1391
Size: 1932 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3209 928
Size: 971 1310 3723
Fragments arranged by size:
3723 1310 971
BbvCI CC'TCA_GC
Cuts at: 2447 2447
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2685 4615 136
Size: 107 122 71 631 605 1013 1930 1525
Fragments arranged by size:
1930 1525 1013 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3260 3421 3260
Size: 161 5843
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2298 2989 4065 5221 1790
Size: 499 9 691 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 691 499 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2096 2096
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2453 4664 1239
Size: 796 418 2211 2579
Fragments arranged by size:
2579 2211 796 418
Bpu10I CC'TnA_GC
Cuts at: 2447 2976 3251 2447
Size: 529 275 5200
Fragments arranged by size:
5200 529 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 3116 4667 882
Size: 617 1362 255 1551 2219
Fragments arranged by size:
2219 1551 1362 617 255
BsaAI yAC'GTr
Cuts at: 493 1204 2281 3795 493
Size: 711 1077 1514 2702
Fragments arranged by size:
2702 1514 1077 711
BsaBI GATnn'nnATC
Cuts at: 2108 5998 2108
Size: 3890 2114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2100 2529 2700 4437 4835 5109 5256 5667 2100
Size: 429 171 1737 398 274 147 411 2437
Fragments arranged by size:
2437 1737 429 411 398 274 171 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3161 3191 1178
Size: 30 165 30 1758 30 3991
Fragments arranged by size:
3991 1758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2327 2354 2767 2921 3035 3095 1186
Size: 868 273 27 413 154 114 60 4095
Fragments arranged by size:
4095 868 413 273 154 114 60 27
BseYI C'CCAG_C
Cuts at: 2172 2344 5207 5703 2172
Size: 172 2863 496 2473
Fragments arranged by size:
2863 2473 496 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2124 3454 4215 5243 1540
Size: 275 186 123 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186 123
BsiHKAI G_wGCw'C
Cuts at: 2055 2298 4065 4150 5221 2055
Size: 243 1767 85 1071 2838
Fragments arranged by size:
2838 1767 1071 243 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 2263 2263
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2289 2298 2989 3323 4065 4150
Size: 399 265 234 9 691 334 742 85
Cuts at: 4150 5221 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 334 265 234
85 9
BspEI T'CCGG_A
Cuts at: 2100 2529 5667 2100
Size: 429 3138 2437
Fragments arranged by size:
3138 2437 429
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3359 844
Size: 2515 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2766 4499 4673 65
Size: 1183 1518 1733 174 1396
Fragments arranged by size:
1733 1518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 4648 4835 1700
Size: 110 746 2092 187 2869
Fragments arranged by size:
2869 2092 746 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 3190 794
Size: 1499 897 3608
Fragments arranged by size:
3608 1499 897
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2578 2180
Size: 398 5606
BstYI r'GATC_y
Cuts at: 1972 2103 2532 2697 3334 3701 4086 4103 5555
Size: 131 429 165 637 367 385 17 1452
Cuts at: 5555 5641 5653 5999 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 637 429 385 367 346 165
131 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2601 3075 513
Size: 552 939 597 474 3442
Fragments arranged by size:
3442 939 597 552 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2876 2960 3520 4314 4334 2876
Size: 84 560 794 20 4546
Fragments arranged by size:
4546 794 560 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DraIII CAC_nnn'GTG
Cuts at: 2293 2293
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 3096 3451
Size: 54 1425 50 275 18 291 975 355
Cuts at: 3451 4340 5969 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 975 889 355 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2121 3451 1537
Size: 275 309 1330 4090
Fragments arranged by size:
4090 1330 309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 2708 2811 3150 3934 1353
Size: 1037 318 103 339 784 3423
Fragments arranged by size:
3423 1037 784 339 318 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 4591 4965 5111 387
Size: 1064 138 762 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 762 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2453 2711 4067 4664 5451 5719
Size: 393 403 418 258 1356 597 787 268
Cuts at: 5719 1239
Size: 1524
Fragments arranged by size:
1524 1356 787 597 418 403 393 268
258
EcoNI CCTnn'n_nnAGG
Cuts at: 2713 2713
Size: 6004
EcoO109I rG'GnC_Cy
Cuts at: 2439 2451 2924 2439
Size: 12 473 5519
Fragments arranged by size:
5519 473 12
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2524 2556 3149
Size: 32 135 32 563 32 357 32 593
Cuts at: 3149 3161 3181 3193 4645 4677 4719 4751 1373
Size: 12 20 12 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 593 563 357 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 3147 5118 5302 1305
Size: 873 969 1971 184 2007
Fragments arranged by size:
2007 1971 969 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3962 4321 4480 518
Size: 648 2796 359 159 2042
Fragments arranged by size:
2796 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2403 2550 3197 3420 4097 5245 5490 1606
Size: 797 147 647 223 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 647 245 223 147
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3362 4907 1132
Size: 97 2133 1545 2229
Fragments arranged by size:
2229 2133 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2734 2929 1348
Size: 1386 195 4423
Fragments arranged by size:
4423 1386 195
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2439 2451 2924 2439
Size: 12 473 5519
Fragments arranged by size:
5519 473 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2183 2215 2183
Size: 32 5972
PstI C_TGCA'G
Cuts at: 830 2247 2555 3019 3356 830
Size: 1417 308 464 337 3478
Fragments arranged by size:
3478 1417 464 337 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 3197 3197
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SanDI GG'GwC_CC
Cuts at: 2439 2439
Size: 6004
SapI GCTCTTCn'nnn_
Cuts at: 2390 3150 2390
Size: 760 5244
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2243 2551 2721 3015 3352 4487 5168
Size: 219 1198 308 170 294 337 1135 681
Cuts at: 5168 5359 826
Size: 191 1471
Fragments arranged by size:
1471 1198 1135 681 337 308 294 219
191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 2685 2685
Size: 6004
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2945 3331 1872
Size: 1073 386 4545
Fragments arranged by size:
4545 1073 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 2719 1517
Size: 693 509 4802
Fragments arranged by size:
4802 693 509
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 2793 3443 513
Size: 552 939 109 680 650 3074
Fragments arranged by size:
3074 939 680 650 552 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2227 2864 4031 4048 4201 4386
Size: 1255 606 41 637 1167 17 153 185
Cuts at: 4386 4831 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1167 637 606 445 185 153
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4250 5962 96
Size: 274 80 33 51 1443 2273 1712 138
Fragments arranged by size:
2273 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2943 3329 1870
Size: 1073 386 4545
Fragments arranged by size:
4545 1073 386
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI RsrII SacI SacII SexAI SfoI
SrfI SwaI XcmI