Restriction map for
pFN21AB7168
(Circular) (Six-base) MAPSORT of: pFN21AB7168.seq Check: 6789 from: 1 to: 5941
pFN21AB7168 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 3284 1937
Size: 889 458 4594
Fragments arranged by size:
4594 889 458
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2602 4004 5388 5656 1632
Size: 970 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 970 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2919 2951 2919
Size: 32 5909
AlwNI CAG_nnn'CTG
Cuts at: 3684 5256 3684
Size: 1572 4369
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2143 3026 3193 3250 3476 3628 5737 1493
Size: 650 883 167 57 226 152 2109 1697
Fragments arranged by size:
2109 1697 883 650 226 167 152 57
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 3266 1870
Size: 73 1323 4545
Fragments arranged by size:
4545 1323 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4717 618
Size: 325 217 627 1475 1455 1842
Fragments arranged by size:
1842 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3260 1391
Size: 1869 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2608 2742 928
Size: 971 351 358 134 4127
Fragments arranged by size:
4127 971 358 351 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 2215 2486 3833 5043 2002
Size: 213 271 1347 1210 2900
Fragments arranged by size:
2900 1347 1210 271 213
BclI T'GATC_A
Cuts at: 1548 2253 1548
Size: 705 5236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 4552 136
Size: 107 122 71 631 605 958 1922 1525
Fragments arranged by size:
1922 1525 958 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 4002 5158 1790
Size: 2212 1156 2573
Fragments arranged by size:
2573 2212 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 2895 3672
Size: 416 53 83 399 24 1064 414 777
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 1064 958 777 416 414 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2977 3208 4601 1239
Size: 766 972 231 1393 2579
Fragments arranged by size:
2579 1393 972 766 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 2417 5935 2417
Size: 3518 2423
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2215 2245 1178
Size: 30 165 30 812 30 4874
Fragments arranged by size:
4874 812 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5941
BseYI C'CCAG_C
Cuts at: 2097 2576 5144 5640 2097
Size: 479 2568 496 2398
Fragments arranged by size:
2568 2398 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2847 3086 1163
Size: 573 1111 239 4018
Fragments arranged by size:
4018 1111 573 239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4002 4087 5158 4002
Size: 85 1071 4785
Fragments arranged by size:
4785 1071 85
BsmI GAATG_Cn'
Cuts at: 2996 3452 3545 2996
Size: 456 93 5392
Fragments arranged by size:
5392 456 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3260 4002 4087 5158 1391
Size: 399 1470 742 85 1071 2174
Fragments arranged by size:
2174 1470 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 2712 3830 5560 2712
Size: 1118 1730 3093
Fragments arranged by size:
3093 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2533 2587 2967 4436 4610 65
Size: 1183 1285 54 380 1469 174 1396
Fragments arranged by size:
1469 1396 1285 1183 380 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 3095 4001 5013 3095
Size: 906 1012 4023
Fragments arranged by size:
4023 1012 906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2333 2831 3271 3638 4023 4040 5492 5578
Size: 361 498 440 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 498 440 385 367 361 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2827 2827
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 513
Size: 552 939 213 4237
Fragments arranged by size:
4237 939 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 4307
Fragments arranged by size:
4307 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2805 3457 4251 4271 2805
Size: 652 794 20 4475
Fragments arranged by size:
4475 794 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2595 2595
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3388 4277 5906
Size: 54 1425 50 275 18 1558 889 1629
Cuts at: 5906 8
Size: 43
Fragments arranged by size:
1629 1558 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3871 1353
Size: 1160 1358 3423
Fragments arranged by size:
3423 1358 1160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2602 2977 3208 4004 4601 5388
Size: 393 373 597 375 231 796 597 787
Cuts at: 5388 5656 1239
Size: 268 1524
Fragments arranged by size:
1524 796 787 597 597 393 375 373
268 231
EcoO109I rG'GnC_Cy
Cuts at: 2473 2473
Size: 5941
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2490 2522 2794 2826 3126
Size: 32 135 32 918 32 272 32 300
Cuts at: 3126 3158 4582 4614 4656 4688 1373
Size: 32 1424 32 42 32 2626
Fragments arranged by size:
2626 1424 918 300 272 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2668 3285 3531 669
Size: 988 281 730 617 246 3079
Fragments arranged by size:
3079 988 730 617 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2861 5055 5239 1305
Size: 1556 2194 184 2007
Fragments arranged by size:
2194 2007 1556 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2352 2786 3899 4258 4417 518
Size: 648 903 283 434 1113 359 159 2042
Fragments arranged by size:
2042 1113 903 648 434 359 283 159
MspA1I CmG'CkG
Cuts at: 1606 3357 4034 5182 5427 1606
Size: 1751 677 1148 245 2120
Fragments arranged by size:
2120 1751 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2217 513
Size: 552 939 213 4237
Fragments arranged by size:
4237 939 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2857 3299 4844 1132
Size: 97 1628 442 1545 2229
Fragments arranged by size:
2229 1628 1545 442 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 2945 1108
Size: 1487 350 4104
Fragments arranged by size:
4104 1487 350
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2206 2238 2206
Size: 32 5909
PstI C_TGCA'G
Cuts at: 830 2995 3293 830
Size: 2165 298 3478
Fragments arranged by size:
3478 2165 298
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2610 2676 2991 3289 4424 5105 5296
Size: 219 1565 66 315 298 1135 681 191
Cuts at: 5296 826
Size: 1471
Fragments arranged by size:
1565 1471 1135 681 315 298 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 3018 3380 513
Size: 552 939 213 801 362 3074
Fragments arranged by size:
3074 939 801 552 362 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 3968 3985 4138 4323 4768 325
Size: 1255 796 1592 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 796 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3052 4187 5899
Size: 274 80 33 51 1443 1075 1135 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1443 1135 1075 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 2404 3277 2404
Size: 873 5068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2788 2068
Size: 720 5221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI