Restriction map for
pFN21AB7166
(Circular) (Six-base) MAPSORT of: pFN21AB7166.seq Check: 155 from: 1 to: 5893
pFN21AB7166 5893 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 5340 5608 1632
Size: 2324 1384 268 1917
Fragments arranged by size:
2324 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 4999
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2548 3636 5208 2548
Size: 1088 1572 3233
Fragments arranged by size:
3233 1572 1088
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2153 3202 3428 3580 5689 1493
Size: 660 1049 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3099 3218 1870
Size: 73 1156 119 4545
Fragments arranged by size:
4545 1156 119 73
BamHI G'GATC_C
Cuts at: 2413 2815 3223 3590 2413
Size: 402 408 367 4716
Fragments arranged by size:
4716 408 402 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2383 2523 3212 1391
Size: 992 140 689 4072
Fragments arranged by size:
4072 992 689 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 3182 928
Size: 971 187 1096 3639
Fragments arranged by size:
3639 1096 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3785 4995 2436
Size: 1349 1210 3334
Fragments arranged by size:
3334 1349 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2070 5888 2070
Size: 3818 2075
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 3954 5110 1790
Size: 2164 1156 2573
Fragments arranged by size:
2573 2164 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3029 4553 1239
Size: 1790 1524 2579
Fragments arranged by size:
2579 1790 1524
Bpu10I CC'TnA_GC
Cuts at: 2484 3039 2484
Size: 555 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 4018 4883 5181 2831
Size: 1187 865 298 3543
Fragments arranged by size:
3543 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 4082 4717 275
Size: 53 83 186 936 2167 382 635 1451
Fragments arranged by size:
2167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2097 4326 4724 4998 5145 5556 2097
Size: 2229 398 274 147 411 2434
Fragments arranged by size:
2434 2229 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2136 2411 3002 1186
Size: 865 85 275 591 4077
Fragments arranged by size:
4077 865 591 275 85
BseYI C'CCAG_C
Cuts at: 2998 3131 5096 5592 2998
Size: 133 1965 496 3299
Fragments arranged by size:
3299 1965 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2650 1163
Size: 573 914 4406
Fragments arranged by size:
4406 914 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 3954 4039 5110 2316
Size: 1638 85 1071 3099
Fragments arranged by size:
3099 1638 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2383 2523 3212 3954 4039 5110
Size: 399 526 67 140 689 742 85 1071
Cuts at: 5110 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 689 526 399 140 85
67
BspEI T'CCGG_A
Cuts at: 2097 5556 2097
Size: 3459 2434
BspHI T'CATG_A
Cuts at: 2523 3782 5512 2523
Size: 1259 1730 2904
Fragments arranged by size:
2904 1730 1259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 2877 4388 4562 65
Size: 1183 1116 513 1511 174 1396
Fragments arranged by size:
1511 1396 1183 1116 513 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2070 2413 2815 3052 3223 3590 3975 3992
Size: 98 343 402 237 171 367 385 17
Cuts at: 3992 5444 5530 5542 5888 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 402 385 367 346 343 237
171 98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3147 3409 4203 4223 3147
Size: 262 794 20 4817
Fragments arranged by size:
4817 794 262 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2643 4900 809
Size: 507 1327 2257 1802
Fragments arranged by size:
2257 1802 1327 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 3340 4229
Size: 54 1425 50 275 18 664 846 889
Cuts at: 4229 5858 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 846 664 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 3823 1353
Size: 1050 1420 3423
Fragments arranged by size:
3423 1420 1050
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3029 3956 4553 5340 5608 1239
Size: 393 1397 927 597 787 268 1524
Fragments arranged by size:
1524 1397 927 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2033
Size: 5893
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 4534 4566 4608
Size: 32 135 32 543 32 2387 32 42
Cuts at: 4608 4640 1373
Size: 32 2626
Fragments arranged by size:
2626 2387 543 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3483 669
Size: 988 281 1299 246 3079
Fragments arranged by size:
3079 1299 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1991 3162 5007 5191 1305
Size: 686 1171 1845 184 2007
Fragments arranged by size:
2007 1845 1171 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2252 3851 4210 4369 518
Size: 648 894 192 1599 359 159 2042
Fragments arranged by size:
2042 1599 894 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2114 3002 3022 3309 3986 5134 5379 1606
Size: 508 888 20 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 508 287 245 20
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3251 4796 1132
Size: 97 2022 1545 2229
Fragments arranged by size:
2229 2022 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4991
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3182 1613
Size: 1569 4324
PsiI TTA'TAA
Cuts at: 2829 3463 2829
Size: 634 5259
PstI C_TGCA'G
Cuts at: 830 2544 3021 3245 830
Size: 1714 477 224 3478
Fragments arranged by size:
3478 1714 477 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
PvuII CAG'CTG
Cuts at: 2114 3002 2114
Size: 888 5005
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2115 2540 3017 3070 3241 4376 5057
Size: 219 1070 425 477 53 171 1135 681
Cuts at: 5057 5248 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1070 681 477 425 219 191
171 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3101 3220 1872
Size: 1229 119 4545
Fragments arranged by size:
4545 1229 119
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 3997 4898 5160 820
Size: 197 926 867 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 2724 3068 1517
Size: 1207 344 4342
Fragments arranged by size:
4342 1207 344
StyI C'CwwG_G
Cuts at: 513 1065 3332 513
Size: 552 2267 3074
Fragments arranged by size:
3074 2267 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2224 3920 3937 4090 4275 4720 325
Size: 1255 644 1696 17 153 185 445 1498
Fragments arranged by size:
1696 1498 1255 644 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 4139 5851
Size: 274 80 33 51 1443 927 1235 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
1712 1443 1235 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 5853 5885 2036
Size: 32 3785 32 2044
Fragments arranged by size:
3785 2044 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 2672 3229 2672
Size: 557 5336
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3099 3218 1870
Size: 1229 119 4545
Fragments arranged by size:
4545 1229 119
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI