Restriction map for
pFN21AB7165
(Circular) (Six-base) MAPSORT of: pFN21AB7165.seq Check: 3516 from: 1 to: 5890
pFN21AB7165 5890 bp
With 182 enzymes: SgfI *
March 28, 2012 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 2933 2389
Size: 544 5346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 1937
Size: 1296 4594
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3953 5337 5605 1632
Size: 413 1908 1384 268 1917
Fragments arranged by size:
1917 1908 1384 413 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 2346 2793 4789 5766 1203
Size: 1143 447 1996 977 1327
Fragments arranged by size:
1996 1327 1143 977 447
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 2210 1166
Size: 1044 4846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4420
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaI G_GGCC'C
Cuts at: 2787 3101 3158 2787
Size: 314 57 5519
Fragments arranged by size:
5519 314 57
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3199 3425 3577 5686 1493
Size: 1706 226 152 2109 1697
Fragments arranged by size:
2109 1706 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 2575 2674 2830 3184 3215 1870
Size: 73 632 99 156 354 31 4545
Fragments arranged by size:
4545 632 354 156 99 73 31
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2494 2653 3211 4666
Size: 325 217 627 516 191 159 558 1455
Cuts at: 4666 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 516 325 217 191
159
BanII G_rGCy'C
Cuts at: 1391 2787 3101 3158 3209 1391
Size: 1396 314 57 51 4072
Fragments arranged by size:
4072 1396 314 57 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2759 3053
Size: 107 122 71 631 605 337 750 294
Cuts at: 3053 4501 136
Size: 1448 1525
Fragments arranged by size:
1525 1448 750 631 605 337 294 122
107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 2369 3173 3307 2369
Size: 804 134 4952
Fragments arranged by size:
4952 804 134
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2476 2499 2787 3101 3158 3951 5107
Size: 516 170 23 288 314 57 793 1156
Cuts at: 5107 1790
Size: 2573
Fragments arranged by size:
2573 1156 793 516 314 288 170 57
23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2180
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2066 2828 2993 4550 1239
Size: 809 18 762 165 1557 2579
Fragments arranged by size:
2579 1557 809 762 165 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4880 5178 4015
Size: 865 298 4727
Fragments arranged by size:
4727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2598 2794 2917 3681 493
Size: 711 1394 196 123 764 2702
Fragments arranged by size:
2702 1394 764 711 196 123
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2317 3028 3101 1186
Size: 1131 711 73 3975
Fragments arranged by size:
3975 1131 711 73
BseYI C'CCAG_C
Cuts at: 2137 2299 2778 3001 3124 5093 5589 2137
Size: 162 479 223 123 1969 496 2438
Fragments arranged by size:
2438 1969 496 479 223 162 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2201 2306 1163
Size: 573 366 99 105 4747
Fragments arranged by size:
4747 573 366 105 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 2411 2688 2716 2754 2843
Size: 275 186 108 302 277 28 38 89
Cuts at: 2843 3340 4101 5129 1540
Size: 497 761 1028 2301
Fragments arranged by size:
2301 1028 761 497 302 277 275 186
108 89 38 28
BsiHKAI G_wGCw'C
Cuts at: 2487 2913 2922 3951 4036 5107 2487
Size: 426 9 1029 85 1071 3270
Fragments arranged by size:
3270 1071 1029 426 85 9
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 3113 2066
Size: 1047 4843
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2306 2476 2487 2499 2787 2913 2922
Size: 399 516 170 11 12 288 126 9
Cuts at: 2922 3101 3158 3209 3951 4036 5107 1391
Size: 179 57 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 399 288 179 170
126 85 57 51 12 11 9
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 2933 3245 844
Size: 1545 544 312 3489
Fragments arranged by size:
3489 1545 544 312
BsrBI CCG'CTC
Cuts at: 1394 1823 2845 3051 3777 1394
Size: 429 1022 206 726 3507
Fragments arranged by size:
3507 1022 726 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2410 2718 4534 4721 1700
Size: 110 600 308 1816 187 2869
Fragments arranged by size:
2869 1816 600 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2290 2846 3950 4962 2290
Size: 556 1104 1012 3218
Fragments arranged by size:
3218 1104 1012 556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2404 2547 794
Size: 1610 143 4137
Fragments arranged by size:
4137 1610 143
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 2200 1184
Size: 1016 4874
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2383
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2196 2280 2436 2442 2710 3092 513
Size: 552 1131 84 156 6 268 382 3311
Fragments arranged by size:
3311 1131 552 382 268 156 84 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2604 2653 487
Size: 683 56 1378 49 3724
Fragments arranged by size:
3724 1378 683 56 49
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2106 2277
Size: 54 1425 50 275 18 180 96 171
Cuts at: 2277 2408 2439 2622 2685 2713 2723 2751 2760
Size: 131 31 183 63 28 10 28 9
Cuts at: 2760 2840 2898 3337 4226 5855 8
Size: 80 58 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 275 183 180 171
131 96 80 63 58 54 50 43
31 28 28 18 10 9
EagI C'GGCC_G
Cuts at: 1537 1812 2106 2408 2685 2713 2751 2840 3337
Size: 275 294 302 277 28 38 89 497
Cuts at: 3337 1537
Size: 4090
Fragments arranged by size:
4090 497 302 294 277 275 89 38
28
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3820 1353
Size: 818 1649 3423
Fragments arranged by size:
3423 1649 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2048 2066 2828 2993 3953 4550
Size: 393 413 3 18 762 165 960 597
Cuts at: 4550 5337 5605 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 787 762 597 413 393 268
165 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2807 2807
Size: 5890
EcoO109I rG'GnC_Cy
Cuts at: 2579 2783 2784 3097 3098 3155 2579
Size: 204 1 313 1 57 5314
Fragments arranged by size:
5314 313 204 57 1 1
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 2942 2963 3136 4396 2942
Size: 21 173 1260 4436
Fragments arranged by size:
4436 1260 173 21
HaeII r_GCGC'y
Cuts at: 1678 2228 2376 2956 3173 5037 1678
Size: 550 148 580 217 1864 2531
Fragments arranged by size:
2531 1864 580 550 217 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2507 2539 4531
Size: 32 135 32 472 32 431 32 1992
Cuts at: 4531 4563 4605 4637 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1992 472 431 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 5004 5188 1305
Size: 951 2748 184 2007
Fragments arranged by size:
2748 2007 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2900 10
Size: 54 1425 343 1068 3000
Fragments arranged by size:
3000 1425 1068 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 3848 4207 4366 518
Size: 648 1044 1638 359 159 2042
Fragments arranged by size:
2042 1638 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2391 2444 2659 2712 3094 3132 3306
Size: 752 33 53 215 53 382 38 174
Cuts at: 3306 3983 5131 5376 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 382 245 215 174
53 53 38 33
NaeI GCC'GGC
Cuts at: 2720 2720
Size: 5890
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5338
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NgoMIV G'CCGG_C
Cuts at: 2718 2718
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 2713 2840 3337 2713
Size: 127 497 5266
Fragments arranged by size:
5266 497 127
NspI r_CATG'y
Cuts at: 1132 1229 2188 2350 2938 3248 4793 1132
Size: 97 959 162 588 310 1545 2229
Fragments arranged by size:
2229 1545 959 588 310 162 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 2346 4789 2346
Size: 2443 3447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 1108
Size: 1089 4801
PfoI T'CCnGG_A
Cuts at: 2043 2511 2043
Size: 468 5422
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PmlI CAC'GTG
Cuts at: 2598 2794 2598
Size: 196 5694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2198 1613
Size: 585 5305
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspOMI G'GGCC_C
Cuts at: 2783 3097 3154 2783
Size: 314 57 5519
Fragments arranged by size:
5519 314 57
PspXI vC'TCGA_Gb
Cuts at: 2674 3184 2674
Size: 510 5380
PstI C_TGCA'G
Cuts at: 830 2400 3077 3242 830
Size: 1570 677 165 3478
Fragments arranged by size:
3478 1570 677 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 3132 3132
Size: 5890
SacII CC_GC'GG
Cuts at: 2445 2713 3095 2445
Size: 268 382 5240
Fragments arranged by size:
5240 382 268
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 2400 3242 2400
Size: 842 5048
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2396 3073 3238 4373 5054 5245 826
Size: 219 1351 677 165 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 677 219 191 165
SfiI GGCCn_nnn'nGGCC
Cuts at: 2759 2759
Size: 5890
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 2718 1700
Size: 1018 4872
SmaI CCC'GGG
Cuts at: 1872 2577 2832 3217 1872
Size: 705 255 385 4545
Fragments arranged by size:
4545 705 385 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2674 3184 3994 4895 5157 820
Size: 197 926 731 510 810 901 262 1553
Fragments arranged by size:
1553 926 901 810 731 510 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 2188 3248 1132
Size: 1056 1060 3774
Fragments arranged by size:
3774 1060 1056
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 3329 513
Size: 552 2264 3074
Fragments arranged by size:
3074 2264 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2993 3917 3934 4087 4272 4717
Size: 1255 620 793 924 17 153 185 445
Cuts at: 4717 325
Size: 1498
Fragments arranged by size:
1498 1255 924 793 620 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4136 5848 96
Size: 274 80 33 51 1443 2159 1712 138
Fragments arranged by size:
2159 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2527
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 2674 3184 1943
Size: 731 510 4649
Fragments arranged by size:
4649 731 510
XmaI C'CCGG_G
Cuts at: 1870 2575 2830 3215 1870
Size: 705 255 385 4545
Fragments arranged by size:
4545 705 385 255
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PsrI RsrII SacI SanDI SapI SexAI SfoI SrfI
SwaI