Restriction map for
pFN21AB7163
(Circular) (Six-base) MAPSORT of: pFN21AB7163.seq Check: 4927 from: 1 to: 5869
pFN21AB7163 5869 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 3212 1937
Size: 939 336 4594
Fragments arranged by size:
4594 939 336
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 2521 2600 2705 2743 2901 3050 3932
Size: 448 441 79 105 38 158 149 882
Cuts at: 3932 5316 5584 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 882 448 441 268 158 149
105 79 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 4768 5745 1203
Size: 3565 977 1327
Fragments arranged by size:
3565 1327 977
AgeI A'CCGG_T
Cuts at: 2618 4700 2618
Size: 2082 3787
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 4414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 3612 5184 3612
Size: 1572 4297
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 2318 2783 3178 3404 3556 5665 1493
Size: 825 465 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 465 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3194 1870
Size: 73 1251 4545
Fragments arranged by size:
4545 1251 73
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 3190 4645 618
Size: 325 217 627 1322 81 1455 1842
Fragments arranged by size:
1842 1455 1322 627 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2037 2151 2208 3188 1391
Size: 646 114 57 980 4072
Fragments arranged by size:
4072 980 646 114 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 928
Size: 971 794 4104
Fragments arranged by size:
4104 971 794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4480 136
Size: 107 122 71 631 605 2808 1525
Fragments arranged by size:
2808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 3286 3286
Size: 5869
Bme1580I G_kGCm'C
Cuts at: 1790 2112 3930 5086 1790
Size: 322 1818 1156 2573
Fragments arranged by size:
2573 1818 1156 322
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 2513 2861
Size: 416 53 83 399 24 689 407 348
Cuts at: 2861 2914 3600 4558 442
Size: 53 686 958 1753
Fragments arranged by size:
1753 958 689 686 416 407 399 348
83 53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2366 2366
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2678 2696 4529 1239
Size: 896 543 18 1833 2579
Fragments arranged by size:
2579 1833 896 543 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 3994 4859 5157 2000
Size: 1994 865 298 2712
Fragments arranged by size:
2712 1994 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 4532 882
Size: 617 597 2436 2219
Fragments arranged by size:
2436 2219 617 597
BsaAI yAC'GTr
Cuts at: 493 1204 3660 493
Size: 711 2456 2702
Fragments arranged by size:
2702 2456 711
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3676 4058 4693 275
Size: 53 83 186 936 2143 382 635 1451
Fragments arranged by size:
2143 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2618 4302 4700 4974 5121 5532 2618
Size: 1684 398 274 147 411 2955
Fragments arranged by size:
2955 1684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 1178
Size: 30 165 30 1147 30 4467
Fragments arranged by size:
4467 1147 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5869
BseYI C'CCAG_C
Cuts at: 5072 5568 5072
Size: 496 5373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 2955 1163
Size: 573 811 408 4077
Fragments arranged by size:
4077 811 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2997 3319 4080 5108 1540
Size: 275 186 996 322 761 1028 2301
Fragments arranged by size:
2301 1028 996 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2151 3930 4015 5086 2037
Size: 114 1779 85 1071 2820
Fragments arranged by size:
2820 1779 1071 114 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2112 2151 2208 3188 3930 4015
Size: 399 247 75 39 57 980 742 85
Cuts at: 4015 5086 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 980 742 399 247 85 75
57 39
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3224 844
Size: 2380 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2561 3756 1394
Size: 429 738 1195 3507
Fragments arranged by size:
3507 1195 738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 4513 4700 1700
Size: 110 808 1895 187 2869
Fragments arranged by size:
2869 1895 808 187 110
BsrGI T'GTAC_A
Cuts at: 96 2841 96
Size: 2745 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 2046 3929 4941 2046
Size: 1883 1012 2974
Fragments arranged by size:
2974 1883 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2656 3092 2623
Size: 33 436 5400
Fragments arranged by size:
5400 436 33
BstYI r'GATC_y
Cuts at: 1972 2019 2884 3199 3566 3951 3968 5420 5506
Size: 47 865 315 367 385 17 1452 86
Cuts at: 5506 5518 5864 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 865 385 367 346 315 86
47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 487
Size: 683 56 1163 3967
Fragments arranged by size:
3967 1163 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 4179 4199 3385
Size: 794 20 5055
Fragments arranged by size:
5055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 2928 2994
Size: 54 1425 50 275 18 663 435 66
Cuts at: 2994 3316 4205 5834 8
Size: 322 889 1629 43
Fragments arranged by size:
1629 1425 889 663 435 322 275 66
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2994 3316 1537
Size: 275 1182 322 4090
Fragments arranged by size:
4090 1182 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2099 3799 1353
Size: 728 18 1700 3423
Fragments arranged by size:
3423 1700 728 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4456 4830 4976 387
Size: 1064 138 2867 374 146 1280
Fragments arranged by size:
2867 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2035 2149 2035
Size: 114 5755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2135 2521 2600 2678 2696 2705
Size: 393 448 55 386 79 78 18 9
Cuts at: 2705 2743 2901 3050 3932 4529 5316 5584 1239
Size: 38 158 149 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 597 448 393 386 268
158 149 79 78 55 38 18 9
EcoO109I rG'GnC_Cy
Cuts at: 2140 2140
Size: 5869
EcoRI G'AATT_C
Cuts at: 2783 3178 3556 2783
Size: 395 378 5096
Fragments arranged by size:
5096 395 378
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 5016 1678
Size: 3338 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373
Size: 32 135 32 2938 32 42 32 2626
Fragments arranged by size:
2938 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3213 3459 669
Size: 988 281 1275 246 3079
Fragments arranged by size:
3079 1275 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 10
Size: 54 1425 343 663 3384
Fragments arranged by size:
3384 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3827 4186 4345 518
Size: 648 1455 1206 359 159 2042
Fragments arranged by size:
2042 1455 1206 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3285 3962 5110 5355 1606
Size: 1679 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 3227 4772 1132
Size: 97 1998 1545 2229
Fragments arranged by size:
2229 1998 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 3096 1108
Size: 1842 146 3881
Fragments arranged by size:
3881 1842 146
PfoI T'CCnGG_A
Cuts at: 2151 2655 2151
Size: 504 5365
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2140
Size: 5869
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PstI C_TGCA'G
Cuts at: 830 3221 830
Size: 2391 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
SacI G_AGCT'C
Cuts at: 2037 2151 2037
Size: 114 5755
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SexAI A'CCwGG_T
Cuts at: 2807 3153 2807
Size: 346 5523
SfcI C'TryA_G
Cuts at: 826 1045 3217 4352 5033 5224 826
Size: 219 2172 1135 681 191 1471
Fragments arranged by size:
2172 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 2618 1700
Size: 918 4951
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3973 4874 5136 820
Size: 197 926 72 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 2747 1517
Size: 1156 74 4639
Fragments arranged by size:
4639 1156 74
StyI C'CwwG_G
Cuts at: 513 1065 3308 513
Size: 552 2243 3074
Fragments arranged by size:
3074 2243 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 3913 4066 4251 4696 325
Size: 1255 2316 17 153 185 445 1498
Fragments arranged by size:
2316 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4115 5827
Size: 274 80 33 51 1443 864 1274 1712
Cuts at: 5827 96
Size: 138
Fragments arranged by size:
1712 1443 1274 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 3058 1316
Size: 1742 4127
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI