Restriction map for
pFN21AB7158
(Circular) (Six-base) MAPSORT of: pFN21AB7158.seq Check: 1228 from: 1 to: 5794
pFN21AB7158 5794 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2420 2420
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2556 3604 278
Size: 53 83 186 936 1020 1048 2468
Fragments arranged by size:
2468 1048 1020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2096 3137 1937
Size: 159 1041 4594
Fragments arranged by size:
4594 1041 159
Acc65I G'GTAC_C
Cuts at: 2430 3115 2430
Size: 685 5109
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2741 2789 3857 5241 5509 1632
Size: 1109 48 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1109 1068 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 2385 4693 5670 1203
Size: 1182 2308 977 1327
Fragments arranged by size:
2308 1327 1182 977
AgeI A'CCGG_T
Cuts at: 2973 4625 2973
Size: 1652 4142
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2154 1184
Size: 970 4824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3046 3078 3046
Size: 32 5762
AlwNI CAG_nnn'CTG
Cuts at: 2783 3537 5109 2783
Size: 754 1572 3468
Fragments arranged by size:
3468 1572 754
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 3103 3329 3481 5590 1493
Size: 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2865 4348 160
Size: 872 1833 1483 1606
Fragments arranged by size:
1833 1606 1483 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2430 3115 4570 618
Size: 325 217 627 643 685 1455 1842
Fragments arranged by size:
1842 1455 685 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3113 1391
Size: 1722 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 2729 928
Size: 971 149 681 3993
Fragments arranged by size:
3993 971 681 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 3855 5011 1790
Size: 2065 1156 2573
Fragments arranged by size:
2573 2065 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4454 1239
Size: 3215 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3919 4784 5082 3919
Size: 865 298 4631
Fragments arranged by size:
4631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3074 4457 882
Size: 617 1575 1383 2219
Fragments arranged by size:
2219 1575 1383 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 3601 3983 4618
Size: 53 83 186 936 1020 1048 382 635
Cuts at: 4618 275
Size: 1451
Fragments arranged by size:
1451 1048 1020 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2973 4227 4625 4899 5046 5457 2973
Size: 1254 398 274 147 411 3310
Fragments arranged by size:
3310 1254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2973 3003 1178
Size: 30 165 30 1570 30 3969
Fragments arranged by size:
3969 1570 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5794
BseYI C'CCAG_C
Cuts at: 2630 4997 5493 2630
Size: 2367 496 2931
Fragments arranged by size:
2931 2367 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3855 3940 5011 3855
Size: 85 1071 4638
Fragments arranged by size:
4638 1071 85
BsmI GAATG_Cn'
Cuts at: 2587 3305 3398 2587
Size: 718 93 4983
Fragments arranged by size:
4983 718 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2544 2544
Size: 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3113 3855 3940 5011 1391
Size: 399 1323 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 3149 844
Size: 1576 729 3489
Fragments arranged by size:
3489 1576 729
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2528 4289 4463 65
Size: 1183 1280 1761 174 1396
Fragments arranged by size:
1761 1396 1280 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2973 4438 4625 1700
Size: 110 1163 1465 187 2869
Fragments arranged by size:
2869 1465 1163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 3018 2920
Size: 98 5696
BstYI r'GATC_y
Cuts at: 1972 3124 3491 3876 3893 5345 5431 5443 5789
Size: 1152 367 385 17 1452 86 12 346
Cuts at: 5789 1972
Size: 1977
Fragments arranged by size:
1977 1452 1152 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3310 4104 4124 3001
Size: 309 794 20 4671
Fragments arranged by size:
4671 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3088 4801 809
Size: 507 1772 1713 1802
Fragments arranged by size:
1802 1772 1713 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3241 4130 5759
Size: 54 1425 50 275 18 1411 889 1629
Cuts at: 5759 8
Size: 43
Fragments arranged by size:
1629 1425 1411 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 3724 1353
Size: 945 1426 3423
Fragments arranged by size:
3423 1426 945
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 2789 3857 4454 5241 5509 1239
Size: 393 1109 48 1068 597 787 268 1524
Fragments arranged by size:
1524 1109 1068 787 597 393 268 48
EcoO109I rG'GnC_Cy
Cuts at: 2225 2225
Size: 5794
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 3029 3061 4435
Size: 32 135 32 597 32 828 32 1374
Cuts at: 4435 4467 4509 4541 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1374 828 597 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 2434 3119 2434
Size: 685 5109
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2947 3004 4908 5092 1305
Size: 1642 57 1904 184 2007
Fragments arranged by size:
2007 1904 1642 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 2414 3752 4111 4270 518
Size: 648 970 278 1338 359 159 2042
Fragments arranged by size:
2042 1338 970 648 359 278 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2630 2780 2937 3210 3887 5035 5280
Size: 446 578 150 157 273 677 1148 245
Cuts at: 5280 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 578 446 273 245 157
150
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5242
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 2389 2694 3152 4697 1132
Size: 97 1160 305 458 1545 2229
Fragments arranged by size:
2229 1545 1160 458 305 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 2385 4693 2385
Size: 2308 3486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5794
PfoI T'CCnGG_A
Cuts at: 2835 2835
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2225 2225
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2630 2780 2937 2630
Size: 150 157 5487
Fragments arranged by size:
5487 157 150
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 3142 4277 4958 5149 826
Size: 219 2097 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3898 4799 5061 820
Size: 197 926 1955 901 262 1553
Fragments arranged by size:
1955 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 2191 2679 3718 5
Size: 47 2139 488 1039 2081
Fragments arranged by size:
2139 2081 1039 488 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 2656 3025 3233 513
Size: 552 1591 369 208 3074
Fragments arranged by size:
3074 1591 552 369 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 3821 3838 3991 4176 4621 325
Size: 1255 1266 975 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 975 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 5754 5786 2740
Size: 32 2982 32 2748
Fragments arranged by size:
2982 2748 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2762 1316
Size: 1446 4348
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2554 3602 276
Size: 53 83 186 936 1020 1048 2468
Fragments arranged by size:
2468 1048 1020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI