Restriction map for
pFN21AB7154
(Circular) (Six-base) MAPSORT of: pFN21AB7154.seq Check: 1745 from: 1 to: 5764
pFN21AB7154 5764 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 3107 1937
Size: 406 764 4594
Fragments arranged by size:
4594 764 406
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2617 3827 5211 5479 1632
Size: 985 1210 1384 268 1917
Fragments arranged by size:
1917 1384 1210 985 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2580 2725 4663 5640 1203
Size: 1377 145 1938 977 1327
Fragments arranged by size:
1938 1377 1327 977 145
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaI G_GGCC'C
Cuts at: 2404 2977 2404
Size: 573 5191
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2482 2685 3073 3299 3451 5560 1493
Size: 989 203 388 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 388 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 1870
Size: 73 1146 4545
Fragments arranged by size:
4545 1146 73
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2404 2977 3083 1391
Size: 1013 573 106 4072
Fragments arranged by size:
4072 1013 573 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2722 928
Size: 971 211 612 3970
Fragments arranged by size:
3970 971 612 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 2349 1548
Size: 801 4963
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2977 3825 4981 1790
Size: 614 573 848 1156 2573
Fragments arranged by size:
2573 1156 848 614 573
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4424 1239
Size: 3185 2579
Bpu10I CC'TnA_GC
Cuts at: 2055 2055
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2257 4427 882
Size: 617 758 2170 2219
Fragments arranged by size:
2219 2170 758 617
BsaAI yAC'GTr
Cuts at: 493 1204 2726 3555 493
Size: 711 1522 829 2702
Fragments arranged by size:
2702 1522 829 711
BsaBI GATnn'nnATC
Cuts at: 2321 2354 5758 2321
Size: 33 3404 2327
Fragments arranged by size:
3404 2327 33
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 4197 4595 4869 5016 5427 2163
Size: 2034 398 274 147 411 2500
Fragments arranged by size:
2500 2034 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 1178
Size: 30 165 30 1632 30 3877
Fragments arranged by size:
3877 1632 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 3056 1186
Size: 871 999 3894
Fragments arranged by size:
3894 999 871
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 3825 3910 4981 2052
Size: 1773 85 1071 2835
Fragments arranged by size:
2835 1773 1071 85
BsmI GAATG_Cn'
Cuts at: 2546 2787 3275 3368 2546
Size: 241 488 93 4942
Fragments arranged by size:
4942 488 241 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3038 3038
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2404 2977 3083 3825 3910 4981
Size: 399 262 352 573 106 742 85 1071
Cuts at: 4981 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 573 399 352 262 106
85
BspEI T'CCGG_A
Cuts at: 2163 5427 2163
Size: 3264 2500
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3119 844
Size: 2275 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2090 2140 2358 3066 4259 4433 65
Size: 1183 842 50 218 708 1193 174 1396
Fragments arranged by size:
1396 1193 1183 842 708 218 174 50
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 3824 4836 3824
Size: 1012 4752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2965 2425
Size: 540 5224
BstYI r'GATC_y
Cuts at: 1972 2082 3094 3461 3846 3863 5315 5401 5413
Size: 110 1012 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1012 385 367 346 110 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3039 3039
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 2592 513
Size: 552 939 97 491 3685
Fragments arranged by size:
3685 939 552 491 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2396 2659 487
Size: 683 56 1170 263 3592
Fragments arranged by size:
3592 1170 683 263 56
BtsI GCAGTG_nn'
Cuts at: 2559 3280 4074 4094 2559
Size: 721 794 20 4229
Fragments arranged by size:
4229 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2427 2595
Size: 54 1425 50 275 18 235 362 168
Cuts at: 2595 3211 4100 5729 8
Size: 616 889 1629 43
Fragments arranged by size:
1629 1425 889 616 362 275 235 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2617 3827 4424 5211 5479 1239
Size: 393 985 1210 597 787 268 1524
Fragments arranged by size:
1524 1210 985 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 2981
Size: 5764
EcoO109I rG'GnC_Cy
Cuts at: 2125 2973 2974 2986 2125
Size: 848 1 12 4903
Fragments arranged by size:
4903 848 12 1
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 4911 1678
Size: 3233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373
Size: 32 135 32 2833 32 42 32 2626
Fragments arranged by size:
2833 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 3108 3354 669
Size: 988 281 244 926 246 3079
Fragments arranged by size:
3079 988 926 281 246 244
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2067 2429 2597 10
Size: 54 1425 343 235 362 168 3177
Fragments arranged by size:
3177 1425 362 343 235 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2293 2591 2792 3722 4081 4240 518
Size: 648 1127 298 201 930 359 159 2042
Fragments arranged by size:
2042 1127 930 648 359 298 201 159
MspA1I CmG'CkG
Cuts at: 1606 2373 2889 3180 3857 5005 5250 1606
Size: 767 516 291 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 516 291 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 3685
Fragments arranged by size:
3685 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2986 2125
Size: 861 4903
PshAI GACnn'nnGTC
Cuts at: 1613 2984 1613
Size: 1371 4393
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2400 2973 2400
Size: 573 5191
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2889 2889
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 2509 4012 5724 2509
Size: 1503 1712 2549
Fragments arranged by size:
2549 1712 1503
SfcI C'TryA_G
Cuts at: 826 1045 2565 3112 4247 4928 5119 826
Size: 219 1520 547 1135 681 191 1471
Fragments arranged by size:
1520 1471 1135 681 547 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 2960 152
Size: 2808 2956
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 2598 2871 3203 513
Size: 552 939 588 6 273 332 3074
Fragments arranged by size:
3074 939 588 552 332 273 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 3791 3808 3961 4146 4591 325
Size: 1255 560 1651 17 153 185 445 1498
Fragments arranged by size:
1651 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2507 4010 5722
Size: 274 80 33 51 1443 530 1503 1712
Cuts at: 5722 96
Size: 138
Fragments arranged by size:
1712 1503 1443 530 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2624 2362
Size: 262 5502
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 2582 3893 2582
Size: 1311 4453
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI