Restriction map for
pFN21AB7153
(Circular) (Six-base) MAPSORT of: pFN21AB7153.seq Check: 1252 from: 1 to: 5764
pFN21AB7153 5764 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2350 2737 3827 5211 5479 1632
Size: 718 387 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1090 718 387 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2868 4663 5640 1203
Size: 1665 1795 977 1327
Fragments arranged by size:
1795 1665 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2714 1166
Size: 1548 4216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2337 2468 3038 1184
Size: 1153 131 570 3910
Fragments arranged by size:
3910 1153 570 131
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2842 3073 3299 3451 5560 1493
Size: 1349 231 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 231 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 2531 2965 3089 1870
Size: 73 382 206 434 124 4545
Fragments arranged by size:
4545 434 382 206 124 73
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2336 3083 1391
Size: 945 747 4072
Fragments arranged by size:
4072 945 747
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2356 928
Size: 971 457 4336
Fragments arranged by size:
4336 971 457
BbvCI CC'TCA_GC
Cuts at: 2297 2297
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2621 4375 136
Size: 107 122 71 631 605 949 1754 1525
Fragments arranged by size:
1754 1525 949 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 3030 1318
Size: 1712 4052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2693 1056
Size: 1637 4127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2557 2113
Size: 444 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2980 3026 3065 4424 1239
Size: 1741 46 39 1359 2579
Fragments arranged by size:
2579 1741 1359 46 39
Bpu10I CC'TnA_GC
Cuts at: 2297 2297
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 2684 3889 4754 5052 2641
Size: 43 1205 865 298 3353
Fragments arranged by size:
3353 1205 865 298 43
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2398 2632 2871 3555 493
Size: 711 1194 234 239 684 2702
Fragments arranged by size:
2702 1194 711 684 239 234
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2306 2724 3571 3953
Size: 53 83 186 936 773 418 847 382
Cuts at: 3953 4588 275
Size: 635 1451
Fragments arranged by size:
1451 936 847 773 635 418 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5764
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 1163
Size: 573 597 4594
Fragments arranged by size:
4594 597 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2909 2927 3214 3975 5003 1540
Size: 275 186 908 18 287 761 1028 2301
Fragments arranged by size:
2301 1028 908 761 287 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2169 3825 3910 4981 2169
Size: 1656 85 1071 2952
Fragments arranged by size:
2952 1656 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 2084 2836 2066
Size: 18 752 4994
Fragments arranged by size:
4994 752 18
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2336 3083 3825 3910 4981 1391
Size: 399 379 167 747 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 379 167 85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2895 3119 844
Size: 2051 224 3489
Fragments arranged by size:
3489 2051 224
BsrBI CCG'CTC
Cuts at: 1394 1823 2655 3651 1394
Size: 429 832 996 3507
Fragments arranged by size:
3507 996 832 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 2211 2403 4259 4433 65
Size: 1183 921 42 192 1856 174 1396
Fragments arranged by size:
1856 1396 1183 921 192 174 42
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2467 3824 4836 2467
Size: 1357 1012 3395
Fragments arranged by size:
3395 1357 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2575 2643 794
Size: 1781 68 3915
Fragments arranged by size:
3915 1781 68
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 2587 2707 1184
Size: 1403 120 4241
Fragments arranged by size:
4241 1403 120
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 3004 3043 2149
Size: 855 39 4870
Fragments arranged by size:
4870 855 39
BstYI r'GATC_y
Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759
Size: 1122 367 385 17 1452 86 12 346
Cuts at: 5759 1972
Size: 1977
Fragments arranged by size:
1977 1452 1122 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 3886
Fragments arranged by size:
3886 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 2921 3280 4074 4094 2321
Size: 600 359 794 20 3991
Fragments arranged by size:
3991 794 600 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2376 2400
Size: 54 1425 50 275 18 399 147 24
Cuts at: 2400 2613 2673 2906 2924 3211 4100 5729 8
Size: 213 60 233 18 287 889 1629 43
Fragments arranged by size:
1629 1425 889 399 287 275 233 213
147 60 54 50 43 24 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2906 2924 3211 1537
Size: 275 1094 18 287 4090
Fragments arranged by size:
4090 1094 287 275 18
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2737 2980 3026 3065 3827 4424
Size: 393 718 387 243 46 39 762 597
Cuts at: 4424 5211 5479 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 718 597 393 387 268
243 46 39
EcoO109I rG'GnC_Cy
Cuts at: 2463 2884 2961 2463
Size: 421 77 5266
Fragments arranged by size:
5266 421 77
EcoRI G'AATT_C
Cuts at: 2842 3073 3451 2842
Size: 231 378 5155
Fragments arranged by size:
5155 378 231
FspI TGC'GCA
Cuts at: 2636 4270 2636
Size: 1634 4130
FspAI rTGC'GCAy
Cuts at: 2636 2636
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2395 4911 1678
Size: 717 2516 2531
Fragments arranged by size:
2531 2516 717
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373
Size: 32 135 32 2833 32 42 32 2626
Fragments arranged by size:
2833 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2402 2615 10
Size: 54 1425 343 546 24 213 3159
Fragments arranged by size:
3159 1425 546 343 213 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2174 2714 3020 3722 4081 4240
Size: 648 981 27 540 306 702 359 159
Cuts at: 4240 518
Size: 2042
Fragments arranged by size:
2042 981 702 648 540 359 306 159
27
MspA1I CmG'CkG
Cuts at: 1606 2911 3180 3857 5005 5250 1606
Size: 1305 269 677 1148 245 2120
Fragments arranged by size:
2120 1305 1148 677 269 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 2689 1052
Size: 1637 4127
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2449 3019 3025 3122 4667 1132
Size: 97 1220 570 6 97 1545 2229
Fragments arranged by size:
2229 1545 1220 570 97 97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PmlI CAC'GTG
Cuts at: 2398 2871 2398
Size: 473 5291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2961 2884
Size: 77 5687
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspXI vC'TCGA_Gb
Cuts at: 2531 2531
Size: 5764
PstI C_TGCA'G
Cuts at: 830 2916 3116 830
Size: 2086 200 3478
Fragments arranged by size:
3478 2086 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SanDI GG'GwC_CC
Cuts at: 2961 2961
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SfcI C'TryA_G
Cuts at: 826 1045 2912 3112 4247 4928 5119 826
Size: 219 1867 200 1135 681 191 1471
Fragments arranged by size:
1867 1471 1135 681 219 200 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2621 2621
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2327 2967 3091 1872
Size: 455 640 124 4545
Fragments arranged by size:
4545 640 455 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 2656 2699 3868 4769 5031
Size: 197 926 588 125 43 1169 901 262
Cuts at: 5031 820
Size: 1553
Fragments arranged by size:
1553 1169 926 901 588 262 197 125
43
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 2449 3019 3122 1132
Size: 1317 570 103 3774
Fragments arranged by size:
3774 1317 570 103
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2537 1517
Size: 1020 4744
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2409 2506 3203 513
Size: 552 939 63 342 97 697 3074
Fragments arranged by size:
3074 939 697 552 342 97 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3049 3791 3808 3961 4146 4591 325
Size: 1255 1469 742 17 153 185 445 1498
Fragments arranged by size:
1498 1469 1255 742 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2016 4010 5722
Size: 274 80 33 51 1443 39 1994 1712
Cuts at: 5722 96
Size: 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 39
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2608 2074
Size: 534 5230
XhoI C'TCGA_G
Cuts at: 1943 2531 1943
Size: 588 5176
XmaI C'CCGG_G
Cuts at: 1870 2325 2965 3089 1870
Size: 455 640 124 4545
Fragments arranged by size:
4545 640 455 124
XmnI GAAnn'nnTTC
Cuts at: 2157 3893 2157
Size: 1736 4028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PsrI PvuII RsrII SacI SacII SapI
SexAI SfoI SrfI SwaI