Restriction map for
pFN21AB7152
(Circular) (Six-base) MAPSORT of: pFN21AB7152.seq Check: 5269 from: 1 to: 5758
pFN21AB7152 5758 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 3821 5205 5473 1632
Size: 965 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 965 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2738 1166
Size: 1572 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 3041 3501 5073 3041
Size: 460 1572 3726
Fragments arranged by size:
3726 1572 460
ApaI G_GGCC'C
Cuts at: 2404 2977 2404
Size: 573 5185
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3083 1870
Size: 73 177 963 4545
Fragments arranged by size:
4545 963 177 73
BamHI G'GATC_C
Cuts at: 2082 3088 3455 2082
Size: 1006 367 4385
Fragments arranged by size:
4385 1006 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2558 2795 3079 4534 618
Size: 325 217 627 771 237 284 1455 1842
Fragments arranged by size:
1842 1455 771 627 325 284 237 217
BanII G_rGCy'C
Cuts at: 1391 2404 2673 2870 2977 3077 1391
Size: 1013 269 197 107 100 4072
Fragments arranged by size:
4072 1013 269 197 107 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 928
Size: 971 436 4351
Fragments arranged by size:
4351 971 436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2798 2977 3819 4975 1790
Size: 614 394 179 842 1156 2573
Fragments arranged by size:
2573 1156 842 614 394 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2987 4418 1239
Size: 1748 1431 2579
Fragments arranged by size:
2579 1748 1431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 2616 4191 4589 4863 5010 5421 2163
Size: 453 1575 398 274 147 411 2500
Fragments arranged by size:
2500 1575 453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2016 2582 1186
Size: 830 566 4362
Fragments arranged by size:
4362 830 566
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2870 3819 3904 4975 2870
Size: 949 85 1071 3653
Fragments arranged by size:
3653 1071 949 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2404 2673 2798 2870 2977 3077 3819
Size: 399 614 269 125 72 107 100 742
Cuts at: 3819 3904 4975 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 399 269 125 107
100 85 72
BspEI T'CCGG_A
Cuts at: 2163 5421 2163
Size: 3258 2500
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2140 2358 2673 3060 4253 4427 65
Size: 1183 892 218 315 387 1193 174 1396
Fragments arranged by size:
1396 1193 1183 892 387 315 218 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 2107 1184
Size: 923 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2965 2263
Size: 702 5056
BstYI r'GATC_y
Cuts at: 1972 2082 2685 3088 3455 3840 3857 5309 5395
Size: 110 603 403 367 385 17 1452 86
Cuts at: 5395 5407 5753 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 603 403 385 367 346 110
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2565 2565
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 2262 513
Size: 552 939 252 6 4009
Fragments arranged by size:
4009 939 552 252 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3274 4068 4088 2733
Size: 541 794 20 4403
Fragments arranged by size:
4403 794 541 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2314 2314
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2259 2427
Size: 54 1425 50 275 18 399 30 168
Cuts at: 2427 2583 3205 4094 5723 8
Size: 156 622 889 1629 43
Fragments arranged by size:
1629 1425 889 622 399 275 168 156
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2868
Size: 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 2987 3821 4418 5205 5473 1239
Size: 393 965 390 834 597 787 268 1524
Fragments arranged by size:
1524 965 834 787 597 393 390 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 2981
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2125 2728 2973 2974 2125
Size: 603 245 1 4909
Fragments arranged by size:
4909 603 245 1
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 2598 2630 4399
Size: 32 135 32 874 32 120 32 1769
Cuts at: 4399 4431 4473 4505 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1769 874 135 120 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3102 3348 669
Size: 988 281 919 245 246 3079
Fragments arranged by size:
3079 988 919 281 246 245
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 2857 3348 2857
Size: 491 5267
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2425 4872 5056 1305
Size: 1120 2447 184 2007
Fragments arranged by size:
2447 2007 1120 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2429 2585 10
Size: 54 1425 343 429 168 156 3183
Fragments arranged by size:
3183 1425 429 343 168 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2315 2435 2591 2738 3716 4075
Size: 648 1095 54 120 156 147 978 359
Cuts at: 4075 4234 518
Size: 159 2042
Fragments arranged by size:
2042 1095 978 648 359 159 156 147
120 54
MspA1I CmG'CkG
Cuts at: 1606 2373 2889 3174 3851 4999 5244 1606
Size: 767 516 285 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 516 285 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2256 2262 513
Size: 552 939 252 6 4009
Fragments arranged by size:
4009 939 552 252 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 3827 3859 5340 5372 2804
Size: 32 991 32 1481 32 3190
Fragments arranged by size:
3190 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2728 2125
Size: 603 5155
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2400 2973 2400
Size: 573 5185
PstI C_TGCA'G
Cuts at: 830 3037 3110 830
Size: 2207 73 3478
Fragments arranged by size:
3478 2207 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2889 2889
Size: 5758
SacI G_AGCT'C
Cuts at: 2870 2870
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2499 3033 3106 4241 4922 5113 826
Size: 219 1454 534 73 1135 681 191 1471
Fragments arranged by size:
1471 1454 1135 681 534 219 191 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2262 2450 3197 513
Size: 552 939 252 6 188 747 3074
Fragments arranged by size:
3074 939 747 552 252 188 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 3785 3802 3955 4140 4585 325
Size: 1255 560 1645 17 153 185 445 1498
Fragments arranged by size:
1645 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2531 2832 4004
Size: 274 80 33 51 1443 554 301 1172
Cuts at: 4004 5716 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 554 301 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI