Restriction map for
pFN21AB7150
(Circular) (Six-base) MAPSORT of: pFN21AB7150.seq Check: 2366 from: 1 to: 5746
pFN21AB7150 5746 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 3089 1937
Size: 987 165 4594
Fragments arranged by size:
4594 987 165
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3809 5193 5461 1632
Size: 896 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 896 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaI G_GGCC'C
Cuts at: 2834 2834
Size: 5746
ApaLI G'TGCA_C
Cuts at: 3003 3803 4959 3003
Size: 800 1156 3790
Fragments arranged by size:
3790 1156 800
ApoI r'AATT_y
Cuts at: 1493 2909 3055 3281 3433 5542 1493
Size: 1416 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1416 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2058 3071 1870
Size: 73 67 48 1013 4545
Fragments arranged by size:
4545 1013 73 67 48
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2397 2736 2834 2842 3065 1391
Size: 1006 339 98 8 223 4072
Fragments arranged by size:
4072 1006 339 223 98 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BbvCI CC'TCA_GC
Cuts at: 2661 2661
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2638 2672 3937 3971 1903
Size: 34 50 34 617 34 1265 34 3678
Fragments arranged by size:
3678 1265 617 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2299 2557 5741 2299
Size: 258 3184 2304
Fragments arranged by size:
3184 2304 258
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2601 2796 2834 3007 3807 4963 1790
Size: 811 195 38 173 800 1156 2573
Fragments arranged by size:
2573 1156 811 800 195 173 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2898 3477 4435
Size: 416 53 83 399 24 1481 579 958
Cuts at: 4435 442
Size: 1753
Fragments arranged by size:
1753 1481 958 579 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2798 4406 1239
Size: 1265 294 1608 2579
Fragments arranged by size:
2579 1608 1265 294
Bpu10I CC'TnA_GC
Cuts at: 2462 2661 2462
Size: 199 5547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2891 3871 4736 5034 2891
Size: 980 865 298 3603
Fragments arranged by size:
3603 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2120 2678 1186
Size: 890 44 558 4254
Fragments arranged by size:
4254 890 558 44
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 2718 3007 3807 3892 4963 2481
Size: 237 289 800 85 1071 3264
Fragments arranged by size:
3264 1071 800 289 237 85
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2481 2601 2718 2736 2796 2834
Size: 399 607 84 120 117 18 60 38
Cuts at: 2834 2842 3007 3065 3807 3892 4963 1391
Size: 8 165 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 399 165 120 117
85 84 60 58 38 18 8
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3034 3101 844
Size: 2190 67 3489
Fragments arranged by size:
3489 2190 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2999 4390 4577 1700
Size: 110 1189 1391 187 2869
Fragments arranged by size:
2869 1391 1189 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 3469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 4172
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2758 2758
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2299 2557 3076 3443 3828 3845 5297 5383
Size: 327 258 519 367 385 17 1452 86
Cuts at: 5383 5395 5741 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 327 258
86 17 12
BstZ17I GTA'TAC
Cuts at: 2925 2925
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2188 513
Size: 552 1123 4071
Fragments arranged by size:
4071 1123 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2880 3193 4082
Size: 54 1425 50 275 18 1050 313 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1050 889 313 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2097 2943 3676 1353
Size: 662 82 846 733 3423
Fragments arranged by size:
3423 846 733 662 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 2528 2798 3809 4406 5193 5461
Size: 393 872 24 270 1011 597 787 268
Cuts at: 5461 1239
Size: 1524
Fragments arranged by size:
1524 1011 872 787 597 393 270 268
24
EcoO109I rG'GnC_Cy
Cuts at: 2330 2830 2330
Size: 500 5246
EcoRI G'AATT_C
Cuts at: 2909 3055 3433 2909
Size: 146 378 5222
Fragments arranged by size:
5222 378 146
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2071 2099 2103 2131 2159
Size: 32 135 32 499 28 4 28 28
Cuts at: 2159 2191 2482 2514 4387 4419 4461 4493 1373
Size: 32 291 32 1873 32 42 32 2626
Fragments arranged by size:
2626 1873 499 291 135 42 32 32
32 32 32 32 28 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2263 2548 3090 3336 669
Size: 988 281 325 285 542 246 3079
Fragments arranged by size:
3079 988 542 325 285 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 4860 5044 1305
Size: 897 2658 184 2007
Fragments arranged by size:
2658 2007 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 10
Size: 54 1425 343 1050 2874
Fragments arranged by size:
2874 1425 1050 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 3704 4063 4222 518
Size: 648 1369 1169 359 159 2042
Fragments arranged by size:
2042 1369 1169 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 3162 3839 4987 5232 1606
Size: 625 931 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 625 245
NcoI C'CATG_G
Cuts at: 513 1065 2188 513
Size: 552 1123 4071
Fragments arranged by size:
4071 1123 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2830 2830
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2010 2010
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3045 3098 830
Size: 2215 53 3478
Fragments arranged by size:
3478 2215 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2231 2231
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2226 2334 3041 3094 4229 4910 5101
Size: 219 1181 108 707 53 1135 681 191
Cuts at: 5101 826
Size: 1471
Fragments arranged by size:
1471 1181 1135 707 681 219 191 108
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 2870 3850 4751 5013 820
Size: 197 926 67 860 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 860 262 197 67
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2188 2883 2976 3012 3185 513
Size: 552 1123 695 93 36 173 3074
Fragments arranged by size:
3074 1123 695 552 173 93 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 3992 5704
Size: 274 80 33 51 1443 396 1619 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1619 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2233 2265 5706 5738 2233
Size: 32 3441 32 2241
Fragments arranged by size:
3441 2241 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 2302 3082 2302
Size: 780 4966
XhoI C'TCGA_G
Cuts at: 1943 2010 1943
Size: 67 5679
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI