Restriction map for
pFN21AB7147
(Circular) (Six-base) MAPSORT of: pFN21AB7147.seq Check: 9787 from: 1 to: 5743
pFN21AB7147 5743 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 2817 2829 3086 1937
Size: 517 363 12 257 4594
Fragments arranged by size:
4594 517 363 257 12
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2951 3806 5190 5458 1632
Size: 1319 855 1384 268 1917
Fragments arranged by size:
1917 1384 1319 855 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 2730 4642 5619 1203
Size: 1527 1912 977 1327
Fragments arranged by size:
1912 1527 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2610 3486 5058 2610
Size: 876 1572 3295
Fragments arranged by size:
3295 1572 876
ApaI G_GGCC'C
Cuts at: 2724 2724
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2465 3052 3278 3430 5539 1493
Size: 972 587 226 152 2109 1697
Fragments arranged by size:
2109 1697 972 587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 1870
Size: 73 1125 4545
Fragments arranged by size:
4545 1125 73
BamHI G'GATC_C
Cuts at: 2631 3073 3440 2631
Size: 442 367 4934
Fragments arranged by size:
4934 442 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 3064 4519 618
Size: 325 217 627 708 569 1455 1842
Fragments arranged by size:
1842 1455 708 627 569 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2724 2895 3062 1391
Size: 700 633 171 167 4072
Fragments arranged by size:
4072 700 633 171 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 2110 928
Size: 971 148 63 4561
Fragments arranged by size:
4561 971 148 63
BbvCI CC'TCA_GC
Cuts at: 2876 2876
Size: 5743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 3635 4845 2002
Size: 1633 1210 2900
Fragments arranged by size:
2900 1633 1210
BclI T'GATC_A
Cuts at: 1548 2595 1548
Size: 1047 4696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2557 4354 136
Size: 107 122 71 631 605 885 1797 1525
Fragments arranged by size:
1797 1525 885 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2498 2724 3804 4960 1790
Size: 708 226 1080 1156 2573
Fragments arranged by size:
2573 1156 1080 708 226
BmgBI CAC'GTC
Cuts at: 1318 2158 1318
Size: 840 4903
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2540 1056
Size: 1484 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2582 4403 1239
Size: 1298 45 1821 2579
Fragments arranged by size:
2579 1821 1298 45
Bpu10I CC'TnA_GC
Cuts at: 2876 2876
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2785 3534 493
Size: 711 1581 749 2702
Fragments arranged by size:
2702 1581 749 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2523 4176 4574 4848 4995 5406 2523
Size: 1653 398 274 147 411 2860
Fragments arranged by size:
2860 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2822 2825 2852 2855 2872
Size: 30 165 30 1419 3 27 3 17
Cuts at: 2872 2902 1178
Size: 30 4019
Fragments arranged by size:
4019 1419 165 30 30 30 27 17
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2680 2830 2833 2893 1186
Size: 1494 150 3 60 4036
Fragments arranged by size:
4036 1494 150 60 3
BseYI C'CCAG_C
Cuts at: 2389 4946 5442 2389
Size: 2557 496 2690
Fragments arranged by size:
2690 2557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2384 3193 3954 4982 1540
Size: 275 186 383 809 761 1028 2301
Fragments arranged by size:
2301 1028 809 761 383 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 3804 3889 4960 2163
Size: 1641 85 1071 2946
Fragments arranged by size:
2946 1641 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2163 2498 2724 2895 3062 3804
Size: 399 301 72 335 226 171 167 742
Cuts at: 3804 3889 4960 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 301 226 171
167 85 72
BspEI T'CCGG_A
Cuts at: 2523 5406 2523
Size: 2883 2860
BspHI T'CATG_A
Cuts at: 2866 3632 5362 2866
Size: 766 1730 3247
Fragments arranged by size:
3247 1730 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2196 2390 2516 3098 844
Size: 1265 87 194 126 582 3489
Fragments arranged by size:
3489 1265 582 194 126 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2963 3630 1394
Size: 429 1140 667 3507
Fragments arranged by size:
3507 1140 667 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2658 4387 4574 1700
Size: 110 848 1729 187 2869
Fragments arranged by size:
2869 1729 848 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2631 2778 3073 3440 3825 3842 5294 5380
Size: 659 147 295 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 659 385 367 346 295 147
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 513
Size: 552 939 147 4105
Fragments arranged by size:
4105 939 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3259 4053 4073 2440
Size: 819 794 20 4110
Fragments arranged by size:
4110 819 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2825 4750 809
Size: 507 1509 1925 1802
Fragments arranged by size:
1925 1802 1509 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2381 2688
Size: 54 1425 50 275 18 318 233 307
Cuts at: 2688 3019 3190 4079 5708 8
Size: 331 171 889 1629 43
Fragments arranged by size:
1629 1425 889 331 318 307 275 233
171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2381 3190 1537
Size: 275 569 809 4090
Fragments arranged by size:
4090 809 569 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2946 3673 1353
Size: 968 625 727 3423
Fragments arranged by size:
3423 968 727 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2582 2951 3806 4403 5190 5458
Size: 393 905 45 369 855 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 905 855 787 597 393 369 268
45
EcoO109I rG'GnC_Cy
Cuts at: 2015 2505 2721 2015
Size: 490 216 5037
Fragments arranged by size:
5037 490 216
EcoRI G'AATT_C
Cuts at: 2465 3052 3430 2465
Size: 587 378 4778
Fragments arranged by size:
4778 587 378
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2140 4890 1678
Size: 462 2750 2531
Fragments arranged by size:
2750 2531 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373
Size: 32 135 32 2812 32 42 32 2626
Fragments arranged by size:
2812 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2818 3087 3333 669
Size: 988 281 880 269 246 3079
Fragments arranged by size:
3079 988 880 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 2730 5619 2730
Size: 2889 2854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3021 10
Size: 54 1425 343 1189 2732
Fragments arranged by size:
2732 1425 1189 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 4060 4219 518
Size: 648 2535 359 159 2042
Fragments arranged by size:
2535 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2327 2593 2975 3159 3836 4984 5229 1606
Size: 721 266 382 184 677 1148 245 2120
Fragments arranged by size:
2120 1148 721 677 382 266 245 184
NaeI GCC'GGC
Cuts at: 2660 2660
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NgoMIV G'CCGG_C
Cuts at: 2658 2658
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 2536 1052
Size: 1484 4259
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2242 3101 4646 1132
Size: 97 1013 859 1545 2229
Fragments arranged by size:
2229 1545 1013 859 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2276 2743 1108
Size: 1168 467 4108
Fragments arranged by size:
4108 1168 467
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2505 2505
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2720 2720
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2136 2447 3095 830
Size: 1306 311 648 3478
Fragments arranged by size:
3478 1306 648 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2327 2975 2327
Size: 648 5095
RsrII CG'GwC_CG
Cuts at: 2965 2965
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 2816 3085 1936
Size: 880 269 4594
Fragments arranged by size:
4594 880 269
SapI GCTCTTCn'nnn_
Cuts at: 2946 2946
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2132 2443 2614 3091 4226 4907 5098
Size: 219 1087 311 171 477 1135 681 191
Cuts at: 5098 826
Size: 1471
Fragments arranged by size:
1471 1135 1087 681 477 311 219 191
171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2242 3101 1132
Size: 1110 859 3774
Fragments arranged by size:
3774 1110 859
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 513
Size: 552 939 1178 3074
Fragments arranged by size:
3074 1178 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 2982 3770 3787 3940 4125 4570
Size: 1255 796 606 788 17 153 185 445
Cuts at: 4570 325
Size: 1498
Fragments arranged by size:
1498 1255 796 788 606 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 5703 5735 2872
Size: 32 2799 32 2880
Fragments arranged by size:
2880 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2498 2498
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI