Restriction map for
pFN21AB7146
(Circular) (Six-base) MAPSORT of: pFN21AB7146.seq Check: 5260 from: 1 to: 5743
pFN21AB7146 5743 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 2061 4472 2061
Size: 2411 3332
AleI CACnn'nnGTG
Cuts at: 1166 2082 1166
Size: 916 4827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2368 3486 5058 2368
Size: 1118 1572 3053
Fragments arranged by size:
3053 1572 1118
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2678 2819 3052 3278 3430 5539 1493
Size: 1185 141 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1185 233 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2058 3068 1870
Size: 73 67 48 1010 4545
Fragments arranged by size:
4545 1010 73 67 48
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2736 2842 3062 1391
Size: 1345 106 220 4072
Fragments arranged by size:
4072 1345 220 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2351 928
Size: 971 452 4320
Fragments arranged by size:
4320 971 452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3934 3968 1903
Size: 34 50 34 1913 34 3678
Fragments arranged by size:
3678 1913 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2128 3635 4845 2128
Size: 1507 1210 3026
Fragments arranged by size:
3026 1507 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 5738 2670
Size: 3068 2675
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2796 3804 4960 1790
Size: 1006 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 1006
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2898 3474 4432
Size: 416 53 83 399 24 1481 576 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1481 958 576 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3027 1056
Size: 1971 3772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 2891 4403 1239
Size: 1559 93 1512 2579
Fragments arranged by size:
2579 1559 1512 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3868 4733 5031 2174
Size: 1694 865 298 2886
Fragments arranged by size:
2886 1694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 4406 882
Size: 617 1116 1791 2219
Fragments arranged by size:
2219 1791 1116 617
BsaAI yAC'GTr
Cuts at: 493 1204 2039 2180 3534 493
Size: 711 835 141 1354 2702
Fragments arranged by size:
2702 1354 835 711 141
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 1178
Size: 30 165 30 1074 30 4414
Fragments arranged by size:
4414 1074 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 1186
Size: 1492 4251
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 2381 2793 1163
Size: 573 600 45 412 4113
Fragments arranged by size:
4113 600 573 412 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 2188 2209 3804 3889 4960 2089
Size: 99 21 1595 85 1071 2872
Fragments arranged by size:
2872 1595 1071 99 85 21
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2188 2209 2736 2796 2842 3062
Size: 399 299 99 21 527 60 46 220
Cuts at: 3062 3804 3889 4960 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 399 299 220 99
85 60 46 21
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2640 3632 5362 2640
Size: 992 1730 3021
Fragments arranged by size:
3021 1730 992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 96
Size: 2283 3460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2583 3803 4815 2583
Size: 1220 1012 3511
Fragments arranged by size:
3511 1220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2457 2557 2670 3073 3440 3825 3842 5294
Size: 485 100 113 403 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 485 403 385 367 346 113
100 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2994 513
Size: 552 1929 3262
Fragments arranged by size:
3262 1929 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 3259 4053 4073 2094
Size: 1165 794 20 3764
Fragments arranged by size:
3764 1165 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 2508 3047 2508
Size: 539 5204
DraIII CAC_nnn'GTG
Cuts at: 2183 2183
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 4750 809
Size: 507 1221 2213 1802
Fragments arranged by size:
2213 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708
Size: 54 1425 50 275 18 1360 889 1629
Cuts at: 5708 8
Size: 43
Fragments arranged by size:
1629 1425 1360 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2922 3037 3673 1353
Size: 662 907 115 636 3423
Fragments arranged by size:
3423 907 662 636 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 2891 3806 4403 5190 5458 1239
Size: 393 1166 93 915 597 787 268 1524
Fragments arranged by size:
1524 1166 915 787 597 393 268 93
EcoO109I rG'GnC_Cy
Cuts at: 2866 2866
Size: 5743
EcoRI G'AATT_C
Cuts at: 2678 2819 3052 3430 2678
Size: 141 233 378 4991
Fragments arranged by size:
4991 378 233 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2464 2734 2305
Size: 159 270 5314
Fragments arranged by size:
5314 270 159
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2059 2079 2091 2566
Size: 32 135 32 475 12 20 12 475
Cuts at: 2566 2598 4384 4416 4458 4490 1373
Size: 32 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 475 475 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2287 2469 2765 748
Size: 1539 182 296 3726
Fragments arranged by size:
3726 1539 296 182
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2037 2082 2486 3701 4060 4219 518
Size: 648 871 45 404 1215 359 159 2042
Fragments arranged by size:
2042 1215 871 648 404 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2994 513
Size: 552 1929 3262
Fragments arranged by size:
3262 1929 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 3023 1052
Size: 1971 3772
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 1108
Size: 1650 4093
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PmlI CAC'GTG
Cuts at: 2039 2039
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2866 2866
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2968 3095 830
Size: 2138 127 3478
Fragments arranged by size:
3478 2138 127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 3037 3037
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2226 2964 3091 4226 4907 5098 826
Size: 219 1181 738 127 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 738 681 219 191 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2060 3070 1872
Size: 188 1010 4545
Fragments arranged by size:
4545 1010 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3847 4748 5010 820
Size: 197 926 246 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2526 2883 2994 3012 3018 3182 513
Size: 552 1461 357 111 18 6 164 3074
Fragments arranged by size:
3074 1461 552 357 164 111 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 3770 3787 3940 4125 4570 325
Size: 1255 1185 1005 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1185 1005 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 3989 5701
Size: 274 80 33 51 1443 402 1610 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1610 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2058 3068 1870
Size: 188 1010 4545
Fragments arranged by size:
4545 1010 188
XmnI GAAnn'nnTTC
Cuts at: 2447 2717 3007 3872 2447
Size: 270 290 865 4318
Fragments arranged by size:
4318 865 290 270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI