Restriction map for
pFN21AB7139
(Circular) (Six-base) MAPSORT of: pFN21AB7139.seq Check: 90 from: 1 to: 5704
pFN21AB7139 5704 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2703 2703
Size: 5704
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 3047 1937
Size: 340 770 4594
Fragments arranged by size:
4594 770 340
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 2149 4433 2149
Size: 2284 3420
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 4378
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaI G_GGCC'C
Cuts at: 2823 2823
Size: 5704
ApaLI G'TGCA_C
Cuts at: 2690 3761 4917 2690
Size: 1071 1156 3477
Fragments arranged by size:
3477 1156 1071
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2526 2762 3025 4480
Size: 325 217 627 675 64 236 263 1455
Cuts at: 4480 618
Size: 1842
Fragments arranged by size:
1842 1455 675 627 325 263 236 217
64
BanII G_rGCy'C
Cuts at: 1391 2553 2823 2856 3023 1391
Size: 1162 270 33 167 4072
Fragments arranged by size:
4072 1162 270 167 33
BbeI G_GCGC'C
Cuts at: 2530 2530
Size: 5704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2627 2785 2901 928
Size: 971 728 158 116 3731
Fragments arranged by size:
3731 971 728 158 116
BbvCI CC'TCA_GC
Cuts at: 2711 2711
Size: 5704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2452 2486 2559 2593 3895 3929 1903
Size: 34 515 34 73 34 1302 34 3678
Fragments arranged by size:
3678 1302 515 73 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 2535 4315
Size: 107 122 71 631 605 452 411 1780
Cuts at: 4315 136
Size: 1525
Fragments arranged by size:
1780 1525 631 605 452 411 122 107
71
BglII A'GATC_T
Cuts at: 2073 2412 5699 2073
Size: 339 3287 2078
Fragments arranged by size:
3287 2078 339
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2350 2465 2694 2731 2765 2823 3765 4921
Size: 560 115 229 37 34 58 942 1156
Cuts at: 4921 1790
Size: 2573
Fragments arranged by size:
2573 1156 942 560 229 115 58 37
34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 2245 2884
Size: 416 53 83 399 24 606 222 639
Cuts at: 2884 3435 4393 442
Size: 551 958 1753
Fragments arranged by size:
1753 958 639 606 551 416 399 222
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2702 4364 1239
Size: 806 657 1662 2579
Fragments arranged by size:
2579 1662 806 657
Bpu10I CC'TnA_GC
Cuts at: 2711 2711
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2527 2906 3511 3893
Size: 53 83 186 936 994 379 605 382
Cuts at: 3893 4528 275
Size: 635 1451
Fragments arranged by size:
1451 994 936 635 605 382 379 186
83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 1178
Size: 30 165 30 1137 30 4312
Fragments arranged by size:
4312 1137 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2758 2909 1186
Size: 1572 151 3981
Fragments arranged by size:
3981 1572 151
BseYI C'CCAG_C
Cuts at: 2175 2972 4907 5403 2175
Size: 797 1935 496 2476
Fragments arranged by size:
2476 1935 797 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 1163
Size: 573 434 4697
Fragments arranged by size:
4697 573 434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2391 3154 3915 4943 1540
Size: 275 186 60 330 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 330 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2055 2091 2455 2484 2575 2694 3765 3850 4921
Size: 36 364 29 91 119 1071 85 1071
Cuts at: 4921 2055
Size: 2838
Fragments arranged by size:
2838 1071 1071 364 119 91 85 36
29
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2091 2350 2455 2465 2484 2553
Size: 399 265 36 259 105 10 19 69
Cuts at: 2553 2575 2694 2731 2765 2823 2856 3023 3765
Size: 22 119 37 34 58 33 167 742
Cuts at: 3765 3850 4921 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 265 259 167 119
105 85 69 58 37 36 34 33
22 19 10
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 2703 3059 844
Size: 1841 18 356 3489
Fragments arranged by size:
3489 1841 356 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2317 2377 3591 1394
Size: 429 494 60 1214 3507
Fragments arranged by size:
3507 1214 494 429 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 2353 4348 4535 1700
Size: 110 313 230 1995 187 2869
Fragments arranged by size:
2869 1995 313 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2588 3764 4776 2588
Size: 1176 1012 3516
Fragments arranged by size:
3516 1176 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2073 2412 3034 3401 3786 3803 5255 5341
Size: 101 339 622 367 385 17 1452 86
Cuts at: 5341 5353 5699 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 622 385 367 346 339 101
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2446 2544 2613 2808 513
Size: 552 939 57 385 98 69 195 3409
Fragments arranged by size:
3409 939 552 385 195 98 69 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2348 3220 4014 4034 2348
Size: 872 794 20 4018
Fragments arranged by size:
4018 872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 4711 809
Size: 507 1284 2111 1802
Fragments arranged by size:
2111 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2355 2388
Size: 54 1425 50 275 18 291 234 33
Cuts at: 2388 2406 2518 2950 3151 4040 5669 8
Size: 18 112 432 201 889 1629 43
Fragments arranged by size:
1629 1425 889 432 291 275 234 201
112 54 50 43 33 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2388 3151 1537
Size: 275 576 763 4090
Fragments arranged by size:
4090 763 576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2258 2307 2930 3634 1353
Size: 743 162 49 623 704 3423
Fragments arranged by size:
3423 743 704 623 162 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2702 3767 4364 5151 5419 1239
Size: 393 413 657 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 787 657 597 413 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2362 2819 2820 2916 2362
Size: 457 1 96 5150
Fragments arranged by size:
5150 457 96 1
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 5704
FspI TGC'GCA
Cuts at: 2441 4210 2441
Size: 1769 3935
HaeII r_GCGC'y
Cuts at: 1678 2384 2530 4851 1678
Size: 706 146 2321 2531
Fragments arranged by size:
2531 2321 706 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 4345 4377 4419
Size: 32 135 32 563 32 2178 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 2178 563 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2526 2526
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2632 4818 5002 1305
Size: 1327 2186 184 2007
Fragments arranged by size:
2186 2007 1327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2520 2952 10
Size: 54 1425 343 576 112 432 2762
Fragments arranged by size:
2762 1425 576 432 343 112 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2645 3662 4021 4180 518
Size: 648 1473 6 1017 359 159 2042
Fragments arranged by size:
2042 1473 1017 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2448 3120 3797 4945 5190 1606
Size: 842 672 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 672 245
NaeI GCC'GGC
Cuts at: 2125 2355 2125
Size: 230 5474
NarI GG'CG_CC
Cuts at: 2527 2527
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 2004 2544 513
Size: 552 939 540 3673
Fragments arranged by size:
3673 939 552 540
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2123 2353 2123
Size: 230 5474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4294
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PfoI T'CCnGG_A
Cuts at: 2022 2046 2928 2022
Size: 24 882 4798
Fragments arranged by size:
4798 882 24
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2819 2819
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2247 3056 830
Size: 1417 809 3478
Fragments arranged by size:
3478 1417 809
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SacII CC_GC'GG
Cuts at: 2449 2449
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SapI GCTCTTCn'nnn_
Cuts at: 2096 2307 2096
Size: 211 5493
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SexAI A'CCwGG_T
Cuts at: 2040 2787 2040
Size: 747 4957
SfcI C'TryA_G
Cuts at: 826 1045 2243 3052 4187 4868 5059 826
Size: 219 1198 809 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 809 681 219 191
SfoI GGC'GCC
Cuts at: 2528 2528
Size: 5704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 1517
Size: 693 5011
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2544 3143 513
Size: 552 939 354 186 599 3074
Fragments arranged by size:
3074 939 599 552 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2227 2281 3731 3748 3901 4086
Size: 1255 417 230 54 1450 17 153 185
Cuts at: 4086 4531 325
Size: 445 1498
Fragments arranged by size:
1498 1450 1255 445 417 230 185 153
54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3950 5662 96
Size: 274 80 33 51 1443 1973 1712 138
Fragments arranged by size:
1973 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 2042 2789 1316
Size: 726 747 4231
Fragments arranged by size:
4231 747 726
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SfiI SrfI SwaI