Restriction map for
pFN21AB7129
(Circular) (Six-base) MAPSORT of: pFN21AB7129.seq Check: 438 from: 1 to: 5653
pFN21AB7129 5653 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2772 2865 2996 1937
Size: 835 93 131 4594
Fragments arranged by size:
4594 835 131 93
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3716 5100 5368 1632
Size: 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2242 3396 4968 2242
Size: 1154 1572 2927
Fragments arranged by size:
2927 1572 1154
ApaI G_GGCC'C
Cuts at: 2261 2261
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2261 2972 1391
Size: 870 711 4072
Fragments arranged by size:
4072 870 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 2273 928
Size: 971 184 190 4308
Fragments arranged by size:
4308 971 190 184
BbvCI CC'TCA_GC
Cuts at: 2675 2675
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2261 2843 3714 4870 1790
Size: 471 582 871 1156 2573
Fragments arranged by size:
2573 1156 871 582 471
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 2914 2962 4313 1239
Size: 1523 152 48 1351 2579
Fragments arranged by size:
2579 1523 1351 152 48
Bpu10I CC'TnA_GC
Cuts at: 2675 2675
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 4316 882
Size: 617 1329 1488 2219
Fragments arranged by size:
2219 1488 1329 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 3444 493
Size: 711 1164 1076 2702
Fragments arranged by size:
2702 1164 1076 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 4798
Fragments arranged by size:
4798 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2713 1186
Size: 1527 4126
BseYI C'CCAG_C
Cuts at: 2059 4856 5352 2059
Size: 2797 496 2360
Fragments arranged by size:
2797 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2899 3714 3799 4870 2899
Size: 815 85 1071 3682
Fragments arranged by size:
3682 1071 815 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2843 2899 2972 3714 3799 4870
Size: 399 471 582 56 73 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 582 471 399 85 73
56
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 3008 844
Size: 1882 282 3489
Fragments arranged by size:
3489 1882 282
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 4148 4322 65
Size: 1183 1515 1385 174 1396
Fragments arranged by size:
1515 1396 1385 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2740 1184
Size: 1556 4097
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2866 2866
Size: 5653
Bsu36I CC'TnA_GG
Cuts at: 2873 2873
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 513
Size: 552 939 21 4141
Fragments arranged by size:
4141 939 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2658 2676 2841 3169 3963 3983 2658
Size: 18 165 328 794 20 4328
Fragments arranged by size:
4328 794 328 165 20 18
ClaI AT'CG_AT
Cuts at: 1554 2465 1554
Size: 911 4742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DraIII CAC_nnn'GTG
Cuts at: 2331 2331
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618
Size: 54 1425 50 275 18 1270 889 1629
Cuts at: 5618 8
Size: 43
Fragments arranged by size:
1629 1425 1270 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 2753 2831 3583 1353
Size: 953 447 78 752 3423
Fragments arranged by size:
3423 953 752 447 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 2914 2962 3716 4313 5100 5368
Size: 393 1130 152 48 754 597 787 268
Cuts at: 5368 1239
Size: 1524
Fragments arranged by size:
1524 1130 787 754 597 393 268 152
48
EcoO109I rG'GnC_Cy
Cuts at: 2257 2539 2715 2257
Size: 282 176 5195
Fragments arranged by size:
5195 282 176
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 2528 2560 4294
Size: 32 135 32 705 32 219 32 1734
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1734 705 219 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 2248 2997 3243 669
Size: 988 281 73 237 749 246 3079
Fragments arranged by size:
3079 988 749 281 246 237 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 2011 3243 2011
Size: 1232 4421
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3611 3970 4129 518
Size: 648 1449 996 359 159 2042
Fragments arranged by size:
2042 1449 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 3069 3746 4894 5139 1606
Size: 822 641 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 641 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2025 513
Size: 552 939 21 4141
Fragments arranged by size:
4141 939 552 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4684
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2697 1108
Size: 1134 455 4064
Fragments arranged by size:
4064 1134 455
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2715 2539
Size: 176 5477
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2257 2257
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2306 2306
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2752 3001 4136 4817 5008 826
Size: 219 1707 249 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 249 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4462
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2800 3092 513
Size: 552 939 21 775 292 3074
Fragments arranged by size:
3074 939 775 552 292 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2073 2376 3680 3697 3850 4035 4480
Size: 1255 493 303 1304 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1304 1255 493 445 303 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2146 2175 3899
Size: 274 80 33 51 1443 169 29 1724
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1724 1712 1443 274 169 138 80 51
33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 5613 5645 2118
Size: 32 3463 32 2126
Fragments arranged by size:
3463 2126 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2278
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI