Restriction map for
pFN21AB7120
(Circular) (Six-base) MAPSORT of: pFN21AB7120.seq Check: 2492 from: 1 to: 5587
pFN21AB7120 5587 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2263 2279 2930 1937
Size: 326 16 651 4594
Fragments arranged by size:
4594 651 326 16
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2559 3650 5034 5302 1632
Size: 735 192 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 735 268 192
AflII C'TTAA_G
Cuts at: 820 1017 2267 820
Size: 197 1250 4140
Fragments arranged by size:
4140 1250 197
AflIII A'CryG_T
Cuts at: 1203 4486 5463 1203
Size: 3283 977 1327
Fragments arranged by size:
3283 1327 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 3330 4902 3330
Size: 1572 4015
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2213 2301 2804 2896 3122 3274 5383 1493
Size: 720 88 503 92 226 152 2109 1697
Fragments arranged by size:
2109 1697 720 503 226 152 92 88
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 4363 618
Size: 325 217 627 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2906 1391
Size: 1515 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3479 4689 2391
Size: 1088 1210 3289
Fragments arranged by size:
3289 1210 1088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 3648 4804 1790
Size: 1858 1156 2573
Fragments arranged by size:
2573 1858 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 4276 442
Size: 416 53 83 399 24 1901 958 1753
Fragments arranged by size:
1901 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4247 1239
Size: 3008 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 882
Size: 617 2751 2219
Fragments arranged by size:
2751 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3378 493
Size: 711 2174 2702
Fragments arranged by size:
2702 2174 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4020 4418 4692 4839 5250 4020
Size: 398 274 147 411 4357
Fragments arranged by size:
4357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5362
Fragments arranged by size:
5362 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 4790 5286 4790
Size: 496 5091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5014
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3648 3733 4804 3648
Size: 85 1071 4431
Fragments arranged by size:
4431 1071 85
BsmI GAATG_Cn'
Cuts at: 3098 3191 3098
Size: 93 5494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2906 3648 3733 4804 1391
Size: 399 1116 742 85 1071 2174
Fragments arranged by size:
2174 1116 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 844
Size: 2098 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3474 1394
Size: 429 1651 3507
Fragments arranged by size:
3507 1651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 4082 4256 65
Size: 1183 1179 1655 174 1396
Fragments arranged by size:
1655 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2695 2917 3284 3669 3686 5138 5224 5236
Size: 723 222 367 385 17 1452 86 12
Cuts at: 5236 5582 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 723 385 367 346 222 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5035
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 3103 3897 3917 3103
Size: 794 20 4773
Fragments arranged by size:
4773 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5552
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552
Size: 54 1425 50 275 18 1204 889 1629
Cuts at: 5552 8
Size: 43
Fragments arranged by size:
1629 1425 1204 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3517 1353
Size: 2164 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2417 4174 4548 4694 387
Size: 1064 138 828 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 828 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2559 3650 4247 5034 5302 1239
Size: 393 735 192 1091 597 787 268 1524
Fragments arranged by size:
1524 1091 787 735 597 393 268 192
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
EcoRV GAT'ATC
Cuts at: 2712 2712
Size: 5587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2033 2645 2856 2033
Size: 612 211 4764
Fragments arranged by size:
4764 612 211
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2727 2759 4228 4260 4302
Size: 32 135 32 1155 32 1469 32 42
Cuts at: 4302 4334 1373
Size: 32 2626
Fragments arranged by size:
2626 1469 1155 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2788 2931 3177 669
Size: 988 281 850 143 246 3079
Fragments arranged by size:
3079 988 850 281 246 143
HindIII A'AGCT_T
Cuts at: 748 2593 748
Size: 1845 3742
HpaI GTT'AAC
Cuts at: 2788 3177 2788
Size: 389 5198
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 3545 3904 4063 518
Size: 648 991 1388 359 159 2042
Fragments arranged by size:
2042 1388 991 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3003 3680 4828 5073 1606
Size: 1397 677 1148 245 2120
Fragments arranged by size:
2120 1397 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5035
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2417 2945 4490 1132
Size: 97 1188 528 1545 2229
Fragments arranged by size:
2229 1545 1188 528 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 4486 4486
Size: 5587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5587
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2939 830
Size: 2109 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 2850 3835 5547 2850
Size: 985 1712 2890
Fragments arranged by size:
2890 1712 985
SfcI C'TryA_G
Cuts at: 826 1045 2079 2108 2139 2174 2875 2935 4070
Size: 219 1034 29 31 35 701 60 1135
Cuts at: 4070 4751 4942 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1034 701 681 219 191 60
35 31 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 3691 4592 4854 820
Size: 197 926 324 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2417 2945 1132
Size: 1285 528 3774
Fragments arranged by size:
3774 1285 528
SspI AAT'ATT
Cuts at: 5 52 2752 3511 5
Size: 47 2700 759 2081
Fragments arranged by size:
2700 2081 759 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 3026 513
Size: 552 1961 3074
Fragments arranged by size:
3074 1961 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 3614 3631 3784 3969 4414 325
Size: 1255 448 1586 17 153 185 445 1498
Fragments arranged by size:
1586 1498 1255 448 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2771 2848 3833
Size: 274 80 33 51 1443 794 77 985
Cuts at: 3833 5545 96
Size: 1712 138
Fragments arranged by size:
1712 1443 985 794 274 138 80 77
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5547 5579 5547
Size: 32 5555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2057 2923 2057
Size: 866 4721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 3716 3716
Size: 5587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI