Restriction map for
pFN21AB7115
(Circular) (Six-base) MAPSORT of: pFN21AB7115.seq Check: 1457 from: 1 to: 5542
pFN21AB7115 5542 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2863 2863
Size: 5542
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3605 4989 5257 1632
Size: 1973 1384 268 1917
Fragments arranged by size:
1973 1917 1384 268
AfeI AGC'GCT
Cuts at: 2573 2573
Size: 5542
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5345
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2222 1184
Size: 1038 4504
AlwNI CAG_nnn'CTG
Cuts at: 2089 2622 3285 4857 2089
Size: 533 663 1572 2774
Fragments arranged by size:
2774 1572 663 533
ApaLI G'TGCA_C
Cuts at: 3599 4755 3599
Size: 1156 4386
ApoI r'AATT_y
Cuts at: 1493 2851 3077 3229 5338 1493
Size: 1358 226 152 2109 1697
Fragments arranged by size:
2109 1697 1358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2812 2867 1870
Size: 73 134 735 55 4545
Fragments arranged by size:
4545 735 134 73 55
BamHI G'GATC_C
Cuts at: 2451 2872 3239 2451
Size: 421 367 4754
Fragments arranged by size:
4754 421 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2443 2517 2863 4318 618
Size: 325 217 627 656 74 346 1455 1842
Fragments arranged by size:
1842 1455 656 627 346 325 217 74
BanII G_rGCy'C
Cuts at: 1391 2861 1391
Size: 1470 4072
BbeI G_GCGC'C
Cuts at: 2521 2521
Size: 5542
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3733 3767 1903
Size: 34 1796 34 3678
Fragments arranged by size:
3678 1796 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 2307 1548
Size: 759 4783
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2705 4153 136
Size: 107 122 71 631 605 1033 1448 1525
Fragments arranged by size:
1525 1448 1033 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2959 2959
Size: 5542
Bme1580I G_kGCm'C
Cuts at: 1790 3603 4759 1790
Size: 1813 1156 2573
Fragments arranged by size:
2573 1813 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3273 4231 442
Size: 416 53 83 399 24 1856 958 1753
Fragments arranged by size:
1856 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2285 4202 1239
Size: 1031 15 1917 2579
Fragments arranged by size:
2579 1917 1031 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 4532 4830 3667
Size: 865 298 4379
Fragments arranged by size:
4379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4205 882
Size: 617 2706 2219
Fragments arranged by size:
2706 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3333 493
Size: 711 2129 2702
Fragments arranged by size:
2702 2129 711
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2518 3349 3731 4366
Size: 53 83 186 936 985 831 382 635
Cuts at: 4366 275
Size: 1451
Fragments arranged by size:
1451 985 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3975 4373 4647 4794 5205 3975
Size: 398 274 147 411 4312
Fragments arranged by size:
4312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 1178
Size: 30 165 30 1329 30 3958
Fragments arranged by size:
3958 1329 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2399 2672 2681 1186
Size: 1081 132 273 9 4047
Fragments arranged by size:
4047 1081 273 132 9
BseYI C'CCAG_C
Cuts at: 2113 2426 2643 2789 4745 5241 2113
Size: 313 217 146 1956 496 2414
Fragments arranged by size:
2414 1956 496 313 217 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2470 1163
Size: 573 734 4235
Fragments arranged by size:
4235 734 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2343 2992 3753 4781 1540
Size: 275 186 342 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2439 2564 3603 3688 4759 2211
Size: 228 125 1039 85 1071 2994
Fragments arranged by size:
2994 1071 1039 228 125 85
BsmI GAATG_Cn'
Cuts at: 3053 3146 3053
Size: 93 5449
BsmBI CGTCTCn'nnnn_
Cuts at: 2273 2273
Size: 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2439 2564 2861 3603 3688 4759
Size: 399 421 228 125 297 742 85 1071
Cuts at: 4759 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 421 399 297 228 125
85
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 3431 5161 3431
Size: 1730 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 844
Size: 2053 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3429 1394
Size: 429 1606 3507
Fragments arranged by size:
3507 1606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4037 4211 65
Size: 1183 2789 174 1396
Fragments arranged by size:
2789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4186 4373 1700
Size: 110 2376 187 2869
Fragments arranged by size:
2869 2376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5542
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5542
BssSI C'ACGA_G
Cuts at: 2512 3602 4614 2512
Size: 1090 1012 3440
Fragments arranged by size:
3440 1090 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2484 2622 794
Size: 1690 138 3714
Fragments arranged by size:
3714 1690 138
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5542
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 5542
BstYI r'GATC_y
Cuts at: 1972 2451 2872 3239 3624 3641 5093 5179 5191
Size: 479 421 367 385 17 1452 86 12
Cuts at: 5191 5537 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 479 421 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2412 2466 513
Size: 552 939 408 54 3589
Fragments arranged by size:
3589 939 552 408 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2032 487
Size: 683 56 806 3997
Fragments arranged by size:
3997 806 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2524 3058 3852 3872 2061
Size: 463 534 794 20 3731
Fragments arranged by size:
3731 794 534 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2316 2340
Size: 54 1425 50 275 18 189 297 24
Cuts at: 2340 2637 2989 3878 5507 8
Size: 297 352 889 1629 43
Fragments arranged by size:
1629 1425 889 352 297 297 275 189
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2340 2989 1537
Size: 275 528 649 4090
Fragments arranged by size:
4090 649 528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3472 1353
Size: 2119 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2678 4129 4503 4649 387
Size: 1064 138 1089 1451 374 146 1280
Fragments arranged by size:
1451 1280 1089 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2285 3605 4202 4989 5257 1239
Size: 393 638 15 1320 597 787 268 1524
Fragments arranged by size:
1524 1320 787 638 597 393 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2252
Size: 5542
EcoO109I rG'GnC_Cy
Cuts at: 2073 2682 2073
Size: 609 4933
EcoRI G'AATT_C
Cuts at: 2851 3229 2851
Size: 378 5164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2557 2344
Size: 213 5329
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 2096 2362 2521 2575 4689 1678
Size: 418 266 159 54 2114 2531
Fragments arranged by size:
2531 2114 418 266 159 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 4183 4215 4257
Size: 32 135 32 1160 32 1419 32 42
Cuts at: 4257 4289 1373
Size: 32 2626
Fragments arranged by size:
2626 1419 1160 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3132 669
Size: 988 281 948 246 3079
Fragments arranged by size:
3079 988 948 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5542
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KasI G'GCGC_C
Cuts at: 2517 2517
Size: 5542
KpnI G_GTAC'C
Cuts at: 2867 2867
Size: 5542
MfeI C'AATT_G
Cuts at: 3141 3141
Size: 5542
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2775 4656 4840 1305
Size: 1470 1881 184 2007
Fragments arranged by size:
2007 1881 1470 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 10
Size: 54 1425 343 189 3531
Fragments arranged by size:
3531 1425 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2477 3500 3859 4018 518
Size: 648 1023 288 1023 359 159 2042
Fragments arranged by size:
2042 1023 1023 648 359 288 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2301 2650 2958 3635 4783 5028 1606
Size: 483 212 349 308 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 483 349 308 245 212
NarI GG'CG_CC
Cuts at: 2518 2518
Size: 5542
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4051
Fragments arranged by size:
4051 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2989 2989
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2482 2900 4445 1132
Size: 97 1253 418 1545 2229
Fragments arranged by size:
2229 1545 1253 418 97
PasI CC'CwG_GG
Cuts at: 1348 2687 1348
Size: 1339 4203
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5542
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 5124 5156 3611
Size: 32 1481 32 3997
Fragments arranged by size:
3997 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2682 2682
Size: 5542
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5542
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PstI C_TGCA'G
Cuts at: 830 2289 2618 2894 830
Size: 1459 329 276 3478
Fragments arranged by size:
3478 1459 329 276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SanDI GG'GwC_CC
Cuts at: 2682 2682
Size: 5542
SbfI CC_TGCA'GG
Cuts at: 2894 2894
Size: 5542
ScaI AGT'ACT
Cuts at: 3790 5502 3790
Size: 1712 3830
SfcI C'TryA_G
Cuts at: 826 1045 2285 2614 2890 4025 4706 4897 826
Size: 219 1240 329 276 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 681 329 276 219 191
SfoI GGC'GCC
Cuts at: 2519 2519
Size: 5542
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2869 1872
Size: 997 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 4547 4809 820
Size: 197 926 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2482 2900 1132
Size: 1350 418 3774
Fragments arranged by size:
3774 1350 418
SspI AAT'ATT
Cuts at: 5 52 3466 5
Size: 47 3414 2081
Fragments arranged by size:
3414 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2004 2981 513
Size: 552 939 977 3074
Fragments arranged by size:
3074 977 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3569 3586 3739 3924 4369 325
Size: 1255 1989 17 153 185 445 1498
Fragments arranged by size:
1989 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3788 5500 96
Size: 274 80 33 51 1443 1811 1712 138
Fragments arranged by size:
1811 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 5502 5534 2436
Size: 32 3034 32 2444
Fragments arranged by size:
3034 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2867 1870
Size: 997 4545
XmnI GAAnn'nnTTC
Cuts at: 3671 3671
Size: 5542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI XcmI