Restriction map for
pFN21AB7113
(Circular) (Six-base) MAPSORT of: pFN21AB7113.seq Check: 5194 from: 1 to: 5515
pFN21AB7113 5515 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2858 1937
Size: 172 749 4594
Fragments arranged by size:
4594 749 172
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 3578 4962 5230 1632
Size: 1143 803 1384 268 1917
Fragments arranged by size:
1917 1384 1143 803 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 5483
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 2840 1870
Size: 73 730 167 4545
Fragments arranged by size:
4545 730 167 73
BamHI G'GATC_C
Cuts at: 2758 2845 3212 2758
Size: 87 367 5061
Fragments arranged by size:
5061 367 87
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2582 2836 4291 618
Size: 325 217 627 529 266 254 1455 1842
Fragments arranged by size:
1842 1455 627 529 325 266 254 217
BanII G_rGCy'C
Cuts at: 1391 2239 2622 2834 1391
Size: 848 383 212 4072
Fragments arranged by size:
4072 848 383 212
BbeI G_GCGC'C
Cuts at: 2586 2586
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 2086 928
Size: 971 122 65 4357
Fragments arranged by size:
4357 971 122 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2664 2698 3706 3740 1903
Size: 34 727 34 1008 34 3678
Fragments arranged by size:
3678 1008 727 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2504 4126 136
Size: 107 122 71 631 605 832 1622 1525
Fragments arranged by size:
1622 1525 832 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2239 3576 4732 1790
Size: 449 1337 1156 2573
Fragments arranged by size:
2573 1337 1156 449
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 4175 1239
Size: 767 2169 2579
Fragments arranged by size:
2579 2169 767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 2287 3306 493
Size: 711 909 174 1019 2702
Fragments arranged by size:
2702 1019 909 711 174
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2583 3322 3704 4339
Size: 53 83 186 936 1050 739 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1050 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 1178
Size: 30 165 30 633 30 4627
Fragments arranged by size:
4627 633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 1186
Size: 1129 4386
BseYI C'CCAG_C
Cuts at: 2747 4718 5214 2747
Size: 1971 496 3048
Fragments arranged by size:
3048 1971 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2167 2267 2435 2510 2642 1163
Size: 573 431 100 168 75 132 4036
Fragments arranged by size:
4036 573 431 168 132 100 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 3576 3661 4732 2622
Size: 954 85 1071 3405
Fragments arranged by size:
3405 1071 954 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2622 2834 3576 3661 4732 1391
Size: 399 449 383 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 383 212 85
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 2726 2870 844
Size: 1610 272 144 3489
Fragments arranged by size:
3489 1610 272 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 2305 3402 1394
Size: 429 214 268 1097 3507
Fragments arranged by size:
3507 1097 429 268 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 2535 4010 4184 65
Size: 1183 1287 1475 174 1396
Fragments arranged by size:
1475 1396 1287 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 4159 4346 1700
Size: 110 503 1846 187 2869
Fragments arranged by size:
2869 1846 503 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2524 2524
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2758 2845 3212 3597 3614 5066 5152 5164
Size: 786 87 367 385 17 1452 86 12
Cuts at: 5164 5510 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 786 385 367 346 87 86
17 12
BstZ17I GTA'TAC
Cuts at: 2110 2110
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 513
Size: 552 939 78 3946
Fragments arranged by size:
3946 939 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2498 3031 3825 3845 2498
Size: 533 794 20 4168
Fragments arranged by size:
4168 794 533 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480
Size: 54 1425 50 275 18 1132 889 1629
Cuts at: 5480 8
Size: 43
Fragments arranged by size:
1629 1425 1132 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 3445 1353
Size: 1088 1004 3423
Fragments arranged by size:
3423 1088 1004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 2578 2689 4102 4476 4622
Size: 1064 138 627 362 111 1413 374 146
Cuts at: 4622 387
Size: 1280
Fragments arranged by size:
1413 1280 1064 627 374 362 146 138
111
EcoICRI GAG'CTC
Cuts at: 2620 2620
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2775 3578 4175 4962 5230 1239
Size: 393 374 769 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 769 597 393 374 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2660 2660
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2026 2236 2644 2026
Size: 210 408 4897
Fragments arranged by size:
4897 408 210
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 5515
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2314 2586 4662 1678
Size: 636 272 2076 2531
Fragments arranged by size:
2531 2076 636 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2582 2582
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 4629 4813 1305
Size: 939 2385 184 2007
Fragments arranged by size:
2385 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 3832 3991 518
Size: 648 2307 359 159 2042
Fragments arranged by size:
2307 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2724 2931 3608 4756 5001 1606
Size: 1118 207 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 245 207
NaeI GCC'GGC
Cuts at: 2315 2315
Size: 5515
NarI GG'CG_CC
Cuts at: 2583 2583
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2313 2313
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2262 2873 4418 1132
Size: 97 1033 611 1545 2229
Fragments arranged by size:
2229 1545 1033 611 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2737 1108
Size: 902 727 3886
Fragments arranged by size:
3886 902 727
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PmlI CAC'GTG
Cuts at: 2287 2287
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2036 2068 3584 3616 5097 5129 2036
Size: 32 1516 32 1481 32 2422
Fragments arranged by size:
2422 1516 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 2644
Size: 5515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5515
PspXI vC'TCGA_Gb
Cuts at: 2673 2673
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2125 2244 2787 2867 830
Size: 1295 119 543 80 3478
Fragments arranged by size:
3478 1295 543 119 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SacI G_AGCT'C
Cuts at: 2622 2622
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 2051 3763 5475 2051
Size: 1712 1712 2091
Fragments arranged by size:
2091 1712 1712
SfcI C'TryA_G
Cuts at: 826 1045 2121 2240 2783 2863 3998 4679 4870
Size: 219 1076 119 543 80 1135 681 191
Cuts at: 4870 826
Size: 1471
Fragments arranged by size:
1471 1135 1076 681 543 219 191 119
80
SfoI GGC'GCC
Cuts at: 2584 2584
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 3619 4520 4782 820
Size: 197 926 730 946 901 262 1553
Fragments arranged by size:
1553 946 926 901 730 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2382 2655 1517
Size: 865 273 4377
Fragments arranged by size:
4377 865 273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2954 513
Size: 552 939 950 3074
Fragments arranged by size:
3074 950 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2812 3542 3559 3712 3897 4342 325
Size: 1255 1232 730 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1232 730 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 3761 5473
Size: 274 80 33 51 1443 72 1712 1712
Cuts at: 5473 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 138 80 72 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 2673 1943
Size: 730 4785
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 2561 3644 2561
Size: 1083 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI NruI
NsiI PacI PfoI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI