Restriction map for
pFN21AB7111
(Circular) (Six-base) MAPSORT of: pFN21AB7111.seq Check: 7545 from: 1 to: 5488
pFN21AB7111 5488 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2499 2499
Size: 5488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2484 2809 2484
Size: 325 5163
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3551 4935 5203 1632
Size: 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 268
AfeI AGC'GCT
Cuts at: 2268 2268
Size: 5488
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 2650 1184
Size: 1002 464 4022
Fragments arranged by size:
4022 1002 464
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5488
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 1870
Size: 73 870 4545
Fragments arranged by size:
4545 870 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2484 2809 4264 618
Size: 325 217 627 697 325 1455 1842
Fragments arranged by size:
1842 1455 697 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 2807 1391
Size: 848 568 4072
Fragments arranged by size:
4072 848 568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2573 928
Size: 971 674 3843
Fragments arranged by size:
3843 971 674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2363 2397 3679 3713 1903
Size: 34 426 34 1282 34 3678
Fragments arranged by size:
3678 1282 426 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BfrBI ATG'CAT
Cuts at: 2284 2284
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2239 3549 4705 1790
Size: 449 1310 1156 2573
Fragments arranged by size:
2573 1310 1156 449
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4177 442
Size: 416 53 83 399 24 1802 958 1753
Fragments arranged by size:
1802 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 4148 1239
Size: 986 1923 2579
Fragments arranged by size:
2579 1923 986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 1178
Size: 30 165 30 633 30 4600
Fragments arranged by size:
4600 633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2343 1186
Size: 1157 4331
BseYI C'CCAG_C
Cuts at: 2263 4691 5187 2263
Size: 2428 496 2564
Fragments arranged by size:
2564 2428 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2006 1163
Size: 573 270 4645
Fragments arranged by size:
4645 573 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2680 3549 3634 4705 2680
Size: 869 85 1071 3463
Fragments arranged by size:
3463 1071 869 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
BsmBI CGTCTCn'nnnn_
Cuts at: 2260 2260
Size: 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2680 2807 3549 3634 4705 1391
Size: 399 449 441 127 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 441 399 127 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 2103 3377 5107 2103
Size: 1274 1730 2484
Fragments arranged by size:
2484 1730 1274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2649 2843 844
Size: 1655 150 194 3489
Fragments arranged by size:
3489 1655 194 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2111 3548 4560 2111
Size: 1437 1012 3039
Fragments arranged by size:
3039 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2168 3004 3798 3818 2168
Size: 836 794 20 3838
Fragments arranged by size:
3838 836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2935 3824
Size: 54 1425 50 275 18 447 658 889
Cuts at: 3824 5453 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 447 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 3418 1353
Size: 977 1088 3423
Fragments arranged by size:
3423 1088 977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 3551 4148 4935 5203 1239
Size: 393 593 1326 597 787 268 1524
Fragments arranged by size:
1524 1326 787 597 593 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2296 2296
Size: 5488
EcoO109I rG'GnC_Cy
Cuts at: 2236 2236
Size: 5488
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2270 4635 1678
Size: 592 2365 2531
Fragments arranged by size:
2531 2365 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 4129 4161 4203
Size: 32 135 32 519 32 2006 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 2006 519 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 2539 2832 3078 669
Size: 988 281 106 495 293 246 3079
Fragments arranged by size:
3079 988 495 293 281 246 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2488 2813 2488
Size: 325 5163
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3219
Fragments arranged by size:
3219 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2585 3446 3805 3964 518
Size: 648 1119 300 861 359 159 2042
Fragments arranged by size:
2042 1119 861 648 359 300 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2904 3581 4729 4974 1606
Size: 639 659 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 659 639 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NsiI A_TGCA'T
Cuts at: 2286 2286
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2288 2614 2846 4391 1132
Size: 97 1059 326 232 1545 2229
Fragments arranged by size:
2229 1545 1059 326 232 97
PasI CC'CwG_GG
Cuts at: 1348 2496 1348
Size: 1148 4340
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4586
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2296 2836 3971 4652 4843 826
Size: 219 1251 540 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 681 540 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2614 2846 1132
Size: 1482 232 3774
Fragments arranged by size:
3774 1482 232
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 513
Size: 552 939 923 3074
Fragments arranged by size:
3074 939 923 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2488 3515 3532 3685 3870 4315 325
Size: 1255 908 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 908 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 2156 1316
Size: 840 4648
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 2345 3617 2345
Size: 1272 4216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI