Restriction map for
pFN21AB7106
(Circular) (Six-base) MAPSORT of: pFN21AB7106.seq Check: 4650 from: 1 to: 5383
pFN21AB7106 5383 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AfeI AGC'GCT
Cuts at: 2268 2268
Size: 5383
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 2190 4282 5259 1203
Size: 987 2092 977 1327
Fragments arranged by size:
2092 1327 987 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 1184
Size: 1002 4381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 5351
AlwNI CAG_nnn'CTG
Cuts at: 2500 2600 3126 4698 2500
Size: 100 526 1572 3185
Fragments arranged by size:
3185 1572 526 100
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5383
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2345 2604 2692 2918 3070 5179 1493
Size: 852 259 88 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 259 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 1870
Size: 73 765 4545
Fragments arranged by size:
4545 765 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 4159 618
Size: 325 217 627 917 1455 1842
Fragments arranged by size:
1842 1455 917 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 2702 1391
Size: 848 463 4072
Fragments arranged by size:
4072 848 463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2455 2566 2573 928
Size: 971 556 111 7 3738
Fragments arranged by size:
3738 971 556 111 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3574 3608 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1637 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2070 5378 2070
Size: 3308 2075
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2239 3444 4600 1790
Size: 228 221 1205 1156 2573
Fragments arranged by size:
2573 1205 1156 228 221
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3114 4072 442
Size: 416 53 83 399 24 1697 958 1753
Fragments arranged by size:
1753 1697 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4043 1239
Size: 2804 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3174 493
Size: 711 1083 887 2702
Fragments arranged by size:
2702 1083 887 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2295 3816 4214 4488 4635 5046 2295
Size: 1521 398 274 147 411 2632
Fragments arranged by size:
2632 1521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2471 2501 1178
Size: 30 165 30 633 30 405 30 4060
Fragments arranged by size:
4060 633 405 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 1186
Size: 1189 4194
BseYI C'CCAG_C
Cuts at: 4586 5082 4586
Size: 496 4887
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4810
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2239 2702 3444 3529 4600 1391
Size: 399 228 221 463 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 228 221 85
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 2586 3272 5002 2586
Size: 686 1730 2967
Fragments arranged by size:
2967 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 844
Size: 1894 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4027 4214 1700
Size: 110 2217 187 2869
Fragments arranged by size:
2869 2217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 2442 4187 2442
Size: 1745 3638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2536 2287
Size: 249 5134
BstYI r'GATC_y
Cuts at: 1972 2070 2713 3080 3465 3482 4934 5020 5032
Size: 98 643 367 385 17 1452 86 12
Cuts at: 5032 5378 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 643 385 367 346 98 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 3337
Fragments arranged by size:
3337 939 555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 2899 3693 3713 2601
Size: 298 794 20 4271
Fragments arranged by size:
4271 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2044 2079 529
Size: 35 1480 35 3833
Fragments arranged by size:
3833 1480 35 35
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2830 3719
Size: 54 1425 50 275 18 447 553 889
Cuts at: 3719 5348 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 553 447 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2306 3313 1353
Size: 740 213 1007 3423
Fragments arranged by size:
3423 1007 740 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3446 4043 4830 5098 1239
Size: 393 1814 597 787 268 1524
Fragments arranged by size:
1814 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2236 2236
Size: 5383
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2270 4530 1678
Size: 592 2260 2531
Fragments arranged by size:
2531 2260 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 4024 4056 4098
Size: 32 135 32 899 32 1521 32 42
Cuts at: 4098 4130 1373
Size: 32 2626
Fragments arranged by size:
2626 1521 899 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4497 4681 1305
Size: 3192 184 2007
Fragments arranged by size:
3192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3114
Fragments arranged by size:
3114 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3341 3700 3859 518
Size: 648 1119 1056 359 159 2042
Fragments arranged by size:
2042 1119 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2497 2799 3476 4624 4869 1606
Size: 639 252 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 302 252 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3892
Fragments arranged by size:
3892 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4481
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PmlI CAC'GTG
Cuts at: 2287 2287
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2036 2068 3452 3484 4965 4997 2036
Size: 32 1384 32 1481 32 2422
Fragments arranged by size:
2422 1481 1384 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5383
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2180 2212 2180
Size: 32 5351
PstI C_TGCA'G
Cuts at: 830 2025 2175 2244 2735 830
Size: 1195 150 69 491 3478
Fragments arranged by size:
3478 1195 491 150 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2021 2171 2240 2731 3866 4547 4738
Size: 219 976 150 69 491 1135 681 191
Cuts at: 4738 826
Size: 1471
Fragments arranged by size:
1471 1135 976 681 491 219 191 150
69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2004 2822 513
Size: 552 939 818 3074
Fragments arranged by size:
3074 939 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 2156 1316
Size: 840 4543
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2497
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI