Restriction map for
pFN21AB7105
(Circular) (Six-base) MAPSORT of: pFN21AB7105.seq Check: 3373 from: 1 to: 5377
pFN21AB7105 5377 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 2386
Size: 5377
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4824 5092 1632
Size: 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 268
AfeI AGC'GCT
Cuts at: 2276 2276
Size: 5377
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 2153 2219 2342 1166
Size: 987 66 123 4201
Fragments arranged by size:
4201 987 123 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2157 2189 2157
Size: 32 5345
AlwNI CAG_nnn'CTG
Cuts at: 3120 4692 3120
Size: 1572 3805
ApaI G_GGCC'C
Cuts at: 2049 2094 2326 2420 2466 2049
Size: 45 232 94 46 4960
Fragments arranged by size:
4960 232 94 46 45
ApaLI G'TGCA_C
Cuts at: 2208 3434 4590 2208
Size: 1226 1156 2995
Fragments arranged by size:
2995 1226 1156
ApoI r'AATT_y
Cuts at: 1493 2395 2686 2912 3064 5173 1493
Size: 902 291 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 2361 2519 2702 1870
Size: 73 325 93 158 183 4545
Fragments arranged by size:
4545 325 183 158 93 73
BamHI G'GATC_C
Cuts at: 2590 2707 3074 2590
Size: 117 367 4893
Fragments arranged by size:
4893 367 117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2226 2261 2698 4153 618
Size: 325 217 627 439 35 437 1455 1842
Fragments arranged by size:
1842 1455 627 439 437 325 217 35
BanII G_rGCy'C
Cuts at: 1391 2049 2094 2256 2326 2420 2466 2558 2588
Size: 658 45 162 70 94 46 92 30
Cuts at: 2588 2696 1391
Size: 108 4072
Fragments arranged by size:
4072 658 162 108 94 92 70 46
45 30
BbeI G_GCGC'C
Cuts at: 2230 2230
Size: 5377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BbvCI CC'TCA_GC
Cuts at: 2386 2386
Size: 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3568 3602 1903
Size: 34 50 34 1547 34 3678
Fragments arranged by size:
3678 1547 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 2089 2126
Size: 107 122 71 631 605 360 57 37
Cuts at: 2126 3988 136
Size: 1862 1525
Fragments arranged by size:
1862 1525 631 605 360 122 107 71
57 37
BglII A'GATC_T
Cuts at: 2631 5372 2631
Size: 2741 2636
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2094 2212 2264 2326 2351 2420 2466
Size: 259 45 118 52 62 25 69 46
Cuts at: 2466 3438 4594 1790
Size: 972 1156 2573
Fragments arranged by size:
2573 1156 972 259 118 69 62 52
46 45 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2650 2044
Size: 606 4771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2001 2453 2591 4037 1239
Size: 762 452 138 1446 2579
Fragments arranged by size:
2579 1446 762 452 138
Bpu10I CC'TnA_GC
Cuts at: 2328 2386 2328
Size: 58 5319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2227 3184 3566 4201
Size: 53 83 186 936 694 957 382 635
Cuts at: 4201 275
Size: 1451
Fragments arranged by size:
1451 957 936 694 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3810 4208 4482 4629 5040 3810
Size: 398 274 147 411 4147
Fragments arranged by size:
4147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5152
Fragments arranged by size:
5152 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2250 1186
Size: 1064 4313
BseYI C'CCAG_C
Cuts at: 2198 2405 2440 2466 2508 4580 5076 2198
Size: 207 35 26 42 2072 496 2499
Fragments arranged by size:
2499 2072 496 207 42 35 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4804
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2185 2212 2453 3438 3523 4594 2185
Size: 27 241 985 85 1071 2968
Fragments arranged by size:
2968 1071 985 241 85 27
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2094 2185 2212 2256 2264 2326
Size: 399 259 45 91 27 44 8 62
Cuts at: 2326 2351 2420 2453 2466 2558 2588 2696 3438
Size: 25 69 33 13 92 30 108 742
Cuts at: 3438 3523 4594 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 108 92 91
85 69 62 45 44 33 30 27
25 13 8
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2732 844
Size: 1542 346 3489
Fragments arranged by size:
3489 1542 346
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4021 4208 1700
Size: 110 2211 187 2869
Fragments arranged by size:
2869 2211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 2617 1184
Size: 1433 3944
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2344 2413 2500 2110
Size: 234 69 87 4987
Fragments arranged by size:
4987 234 87 69
BstYI r'GATC_y
Cuts at: 1972 2590 2631 2707 3074 3459 3476 4928 5014
Size: 618 41 76 367 385 17 1452 86
Cuts at: 5014 5026 5372 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 618 385 367 346 86 76
41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2069 2256 2612 513
Size: 552 1004 187 356 3278
Fragments arranged by size:
3278 1004 552 356 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 2893 3687 3707 2078
Size: 815 794 20 3748
Fragments arranged by size:
3748 815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2576 2824
Size: 54 1425 50 275 18 288 458 248
Cuts at: 2824 3713 5342 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 458 288 275 248 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 3307 1353
Size: 788 1166 3423
Fragments arranged by size:
3423 1166 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 2177 3964 4338 4484 387
Size: 1064 138 576 12 1787 374 146 1280
Fragments arranged by size:
1787 1280 1064 576 374 146 138 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2453 2591 3440 4037 4824 5092
Size: 393 369 452 138 849 597 787 268
Cuts at: 5092 1239
Size: 1524
Fragments arranged by size:
1524 849 787 597 452 393 369 268
138
EcoO109I rG'GnC_Cy
Cuts at: 2045 2046 2091 2323 2417 2437 2462 2463 2484
Size: 1 45 232 94 20 25 1 21
Cuts at: 2484 2602 2045
Size: 118 4820
Fragments arranged by size:
4820 232 118 94 45 25 21 20
1 1
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 2230 2278 4524 1678
Size: 552 48 2246 2531
Fragments arranged by size:
2531 2246 552 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2575 2607 4018 4050 4092
Size: 32 135 32 1003 32 1411 32 42
Cuts at: 4092 4124 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 1003 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 2721 2967 669
Size: 988 281 75 708 246 3079
Fragments arranged by size:
3079 988 708 281 246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KasI G'GCGC_C
Cuts at: 2226 2226
Size: 5377
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 4675 1305
Size: 3186 184 2007
Fragments arranged by size:
3186 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 3267
Fragments arranged by size:
3267 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2219 2342 3335 3694 3853 518
Size: 648 987 66 123 993 359 159 2042
Fragments arranged by size:
2042 993 987 648 359 159 123 66
MspA1I CmG'CkG
Cuts at: 1606 2153 2575 2793 3470 4618 4863 1606
Size: 547 422 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 547 422 245 218
NarI GG'CG_CC
Cuts at: 2227 2227
Size: 5377
NcoI C'CATG_G
Cuts at: 513 1065 2069 2256 513
Size: 552 1004 187 3634
Fragments arranged by size:
3634 1004 552 187
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2735 4280 1132
Size: 97 1506 1545 2229
Fragments arranged by size:
2229 1545 1506 97
PasI CC'CwG_GG
Cuts at: 1348 2472 1348
Size: 1124 4253
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 2437
Size: 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2045 2090 2322 2416 2462 2045
Size: 45 232 94 46 4960
Fragments arranged by size:
4960 232 94 46 45
PstI C_TGCA'G
Cuts at: 830 2729 830
Size: 1899 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SapI GCTCTTCn'nnn_
Cuts at: 2141 2141
Size: 5377
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2725 3860 4541 4732 826
Size: 219 1680 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2228 2228
Size: 5377
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2735 1132
Size: 1603 3774
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 2499 1517
Size: 982 4395
StyI C'CwwG_G
Cuts at: 513 1065 2069 2256 2622 2816 513
Size: 552 1004 187 366 194 3074
Fragments arranged by size:
3074 1004 552 366 194 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 2354 3404 3421 3574 3759 4204
Size: 1255 647 127 1050 17 153 185 445
Cuts at: 4204 325
Size: 1498
Fragments arranged by size:
1498 1255 1050 647 445 185 153 127
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2582 2614 5337 5369 2582
Size: 32 2723 32 2590
Fragments arranged by size:
2723 2590 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2155
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI