Restriction map for
pFN21AB7102
(Circular) (Six-base) MAPSORT of: pFN21AB7102.seq Check: 8992 from: 1 to: 5356
pFN21AB7102 5356 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2496 3166 278
Size: 53 83 186 936 960 670 2468
Fragments arranged by size:
2468 960 936 670 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2215 2699 1937
Size: 278 484 4594
Fragments arranged by size:
4594 484 278
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 3419 4803 5071 1632
Size: 454 1333 1384 268 1917
Fragments arranged by size:
1917 1384 1333 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 2345 4085 2345
Size: 1740 3616
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 2459 3099 4671 2459
Size: 640 1572 3144
Fragments arranged by size:
3144 1572 640
ApaI G_GGCC'C
Cuts at: 2168 2196 2168
Size: 28 5328
ApaLI G'TGCA_C
Cuts at: 3413 4569 3413
Size: 1156 4200
ApoI r'AATT_y
Cuts at: 1493 2665 2891 3043 5152 1493
Size: 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2386 2681 1870
Size: 73 333 110 295 4545
Fragments arranged by size:
4545 333 295 110 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2677 4132 618
Size: 325 217 627 890 1455 1842
Fragments arranged by size:
1842 1455 890 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2168 2196 2675 1391
Size: 739 38 28 479 4072
Fragments arranged by size:
4072 739 479 38 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BbvCI CC'TCA_GC
Cuts at: 2460 2460
Size: 5356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2575 3248 4458 2575
Size: 673 1210 3473
Fragments arranged by size:
3473 1210 673
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3967 136
Size: 107 122 71 631 605 2295 1525
Fragments arranged by size:
2295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5351 5351
Size: 5356
BlpI GC'TnA_GC
Cuts at: 2773 2773
Size: 5356
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2196 3417 4573 1790
Size: 378 28 1221 1156 2573
Fragments arranged by size:
2573 1221 1156 378 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 3087 4045
Size: 416 53 83 399 24 754 916 958
Cuts at: 4045 442
Size: 1753
Fragments arranged by size:
1753 958 916 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 2309 4016 1239
Size: 958 112 1707 2579
Fragments arranged by size:
2579 1707 958 112
Bpu10I CC'TnA_GC
Cuts at: 2460 2460
Size: 5356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3481 4346 4644 3481
Size: 865 298 4193
Fragments arranged by size:
4193 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 3163 3545 4180
Size: 53 83 186 936 960 670 382 635
Cuts at: 4180 275
Size: 1451
Fragments arranged by size:
1451 960 936 670 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3789 4187 4461 4608 5019 3789
Size: 398 274 147 411 4126
Fragments arranged by size:
4126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2598 2628 1178
Size: 30 165 30 1195 30 3906
Fragments arranged by size:
3906 1195 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5356
BseYI C'CCAG_C
Cuts at: 2225 4559 5055 2225
Size: 2334 496 2526
Fragments arranged by size:
2526 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4783
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2574 3417 3502 4573 2130
Size: 444 843 85 1071 2913
Fragments arranged by size:
2913 1071 843 444 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2168 2196 2574 2675 3417 3502
Size: 399 340 38 28 378 101 742 85
Cuts at: 3502 4573 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 378 340 101 85
38 28
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2711 844
Size: 1867 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2263 3851 4025 65
Size: 1183 1015 1588 174 1396
Fragments arranged by size:
1588 1396 1183 1015 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2485 4000 4187 1700
Size: 110 675 1515 187 2869
Fragments arranged by size:
2869 1515 675 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5356
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 2011 1184
Size: 827 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 2333 2333
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2239 2426 2464 2686 3053 3438 3455 4907
Size: 267 187 38 222 367 385 17 1452
Cuts at: 4907 4993 5005 5351 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 267 222 187
86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2318 2318
Size: 5356
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4804
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4617
Fragments arranged by size:
4617 683 56
BtsI GCAGTG_nn'
Cuts at: 2460 2872 3666 3686 2460
Size: 412 794 20 4130
Fragments arranged by size:
4130 794 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 2803 3692
Size: 54 1425 50 275 18 583 390 889
Cuts at: 3692 5321 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 583 390 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3286 1353
Size: 1933 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3943 4317 4463 387
Size: 1064 138 2354 374 146 1280
Fragments arranged by size:
2354 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2197 2309 3419 4016 4803 5071
Size: 393 454 111 112 1110 597 787 268
Cuts at: 5071 1239
Size: 1524
Fragments arranged by size:
1524 1110 787 597 454 393 268 112
111
EcoO109I rG'GnC_Cy
Cuts at: 2192 2193 2521 2192
Size: 1 328 5027
Fragments arranged by size:
5027 328 1
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 4503 1678
Size: 2825 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2331 2363 3997 4029 4071
Size: 32 135 32 759 32 1634 32 42
Cuts at: 4071 4103 1373
Size: 32 2626
Fragments arranged by size:
2626 1634 759 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2216 2491 2563 2700 2946 669
Size: 988 281 278 275 72 137 246 3079
Fragments arranged by size:
3079 988 281 278 275 246 137 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2269 2584 4470 4654 1305
Size: 964 315 1886 184 2007
Fragments arranged by size:
2007 1886 964 315 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 10
Size: 54 1425 343 583 2951
Fragments arranged by size:
2951 1425 583 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 3673 3832 518
Size: 648 2148 359 159 2042
Fragments arranged by size:
2148 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2772 3449 4597 4842 1606
Size: 1166 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4804
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2510 2714 4259 1132
Size: 97 1281 204 1545 2229
Fragments arranged by size:
2229 1545 1281 204 97
PasI CC'CwG_GG
Cuts at: 1348 2142 1348
Size: 794 4562
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5356
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 5356
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PspOMI G'GGCC_C
Cuts at: 2164 2192 2164
Size: 28 5328
PstI C_TGCA'G
Cuts at: 830 2505 2708 830
Size: 1675 203 3478
Fragments arranged by size:
3478 1675 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2214 2698 1936
Size: 278 484 4594
Fragments arranged by size:
4594 484 278
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SfcI C'TryA_G
Cuts at: 826 1045 2501 2704 3839 4520 4711 826
Size: 219 1456 203 1135 681 191 1471
Fragments arranged by size:
1471 1456 1135 681 219 203 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2388 2683 1872
Size: 516 295 4545
Fragments arranged by size:
4545 516 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3460 4361 4623 820
Size: 197 926 1517 901 262 1553
Fragments arranged by size:
1553 1517 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2437 2795 513
Size: 552 1372 358 3074
Fragments arranged by size:
3074 1372 552 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3400 3553 3738 4183 325
Size: 1255 1803 17 153 185 445 1498
Fragments arranged by size:
1803 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3602 5314 96
Size: 274 80 33 51 1443 1625 1712 138
Fragments arranged by size:
1712 1625 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5316 5348 5316
Size: 32 5324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2386 2681 1870
Size: 516 295 4545
Fragments arranged by size:
4545 516 295
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2494 3164 276
Size: 53 83 186 936 960 670 2468
Fragments arranged by size:
2468 960 936 670 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI