Restriction map for
pFN21AB7094
(Circular) (Six-base) MAPSORT of: pFN21AB7094.seq Check: 2063 from: 1 to: 5263
pFN21AB7094 5263 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2520 2520
Size: 5263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3073 278
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 1937
Size: 669 4594
Acc65I G'GTAC_C
Cuts at: 2584 2584
Size: 5263
AclI AA'CG_TT
Cuts at: 1563 3390 3763 1563
Size: 1827 373 3063
Fragments arranged by size:
3063 1827 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 2228 3326 4710 4978 1632
Size: 367 229 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 367 268 229
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5066
AflIII A'CryG_T
Cuts at: 1203 2439 4162 5139 1203
Size: 1236 1723 977 1327
Fragments arranged by size:
1723 1327 1236 977
AgeI A'CCGG_T
Cuts at: 4094 4094
Size: 5263
AhdI GACnn_n'nnGTC
Cuts at: 3992 3992
Size: 5263
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 1184
Size: 948 4315
AlwNI CAG_nnn'CTG
Cuts at: 2523 3006 4578 2523
Size: 483 1572 3208
Fragments arranged by size:
3208 1572 483
ApaLI G'TGCA_C
Cuts at: 3320 4476 3320
Size: 1156 4107
ApoI r'AATT_y
Cuts at: 1493 2572 2798 2950 5059 1493
Size: 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3817 160
Size: 872 2785 1606
Fragments arranged by size:
2785 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
AvaI C'yCGr_G
Cuts at: 1870 1943 2366 2588 1870
Size: 73 423 222 4545
Fragments arranged by size:
4545 423 222 73
BamHI G'GATC_C
Cuts at: 2593 2960 2593
Size: 367 4896
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2584 4039 618
Size: 325 217 627 797 1455 1842
Fragments arranged by size:
1842 1455 797 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2499 2582 1391
Size: 1108 83 4072
Fragments arranged by size:
4072 1108 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3454 3488 1903
Size: 34 1517 34 3678
Fragments arranged by size:
3678 1517 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3155 4365 3155
Size: 1210 4053
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 3874 136
Size: 107 122 71 631 605 631 1571 1525
Fragments arranged by size:
1571 1525 631 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5258 5258
Size: 5263
BlpI GC'TnA_GC
Cuts at: 2680 2680
Size: 5263
Bme1580I G_kGCm'C
Cuts at: 1790 3324 4480 1790
Size: 1534 1156 2573
Fragments arranged by size:
2573 1534 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2994 3952 442
Size: 416 53 83 399 24 1577 958 1753
Fragments arranged by size:
1753 1577 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2049
Size: 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3923 1239
Size: 2684 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3388 4253 4551 2168
Size: 1220 865 298 2880
Fragments arranged by size:
2880 1220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3926 882
Size: 617 2427 2219
Fragments arranged by size:
2427 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3054 493
Size: 711 1236 614 2702
Fragments arranged by size:
2702 1236 711 614
BsaBI GATnn'nnATC
Cuts at: 5257 5257
Size: 5263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 3070 3452 4087
Size: 53 83 186 936 536 1001 382 635
Cuts at: 4087 275
Size: 1451
Fragments arranged by size:
1451 1001 936 635 536 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3696 4094 4368 4515 4926 3696
Size: 398 274 147 411 4033
Fragments arranged by size:
4033 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5038
Fragments arranged by size:
5038 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 1186
Size: 1078 4185
BseYI C'CCAG_C
Cuts at: 2212 2239 4466 4962 2212
Size: 27 2227 496 2513
Fragments arranged by size:
2513 2227 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 1163
Size: 573 633 4057
Fragments arranged by size:
4057 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2307 2341 2428 2713 3474 4502
Size: 275 186 306 34 87 285 761 1028
Cuts at: 4502 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 306 285 275 186 87
34
BsiHKAI G_wGCw'C
Cuts at: 3324 3409 4480 3324
Size: 85 1071 4107
Fragments arranged by size:
4107 1071 85
BsmI GAATG_Cn'
Cuts at: 2774 2867 2774
Size: 93 5170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2499 2582 3324 3409 4480 1391
Size: 399 709 83 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 709 399 85 83
BspEI T'CCGG_A
Cuts at: 4926 4926
Size: 5263
BspHI T'CATG_A
Cuts at: 3152 4882 3152
Size: 1730 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2520 2618 844
Size: 1544 132 98 3489
Fragments arranged by size:
3489 1544 132 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2505 3150 1394
Size: 429 682 645 3507
Fragments arranged by size:
3507 682 645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3758 3932 65
Size: 1183 2510 174 1396
Fragments arranged by size:
2510 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2340 3907 4094 1700
Size: 110 530 1567 187 2869
Fragments arranged by size:
2869 1567 530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5263
BssHII G'CGCG_C
Cuts at: 1925 2285 1925
Size: 360 4903
BssSI C'ACGA_G
Cuts at: 3323 4335 3323
Size: 1012 4251
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2523 794
Size: 1729 3534
BstBI TT'CG_AA
Cuts at: 4067 4067
Size: 5263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5263
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2080 2029
Size: 51 5212
BstYI r'GATC_y
Cuts at: 1972 2520 2593 2960 3345 3362 4814 4900 4912
Size: 548 73 367 385 17 1452 86 12
Cuts at: 4912 5258 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2394 2422 513
Size: 552 939 390 28 3354
Fragments arranged by size:
3354 939 552 390 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4524
Fragments arranged by size:
4524 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 2116 2779 3573 3593 2085
Size: 31 663 794 20 3755
Fragments arranged by size:
3755 794 663 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5228
DraI TTT'AAA
Cuts at: 2567 2567
Size: 5263
DraIII CAC_nnn'GTG
Cuts at: 2323 2323
Size: 5263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2416 4270 809
Size: 507 1100 1854 1802
Fragments arranged by size:
1854 1802 1100 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2295 2304
Size: 54 1425 50 275 18 255 210 9
Cuts at: 2304 2352 2425 2446 2710 3599 5228 8
Size: 48 73 21 264 889 1629 43
Fragments arranged by size:
1629 1425 889 275 264 255 210 73
54 50 48 43 21 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2304 2425 2710 1537
Size: 275 492 121 285 4090
Fragments arranged by size:
4090 492 285 275 121
EarI CTCTTCn'nnn_
Cuts at: 1353 3193 1353
Size: 1840 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2264 3850 4224 4370 387
Size: 1064 138 675 1586 374 146 1280
Fragments arranged by size:
1586 1280 1064 675 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2228 3326 3923 4710 4978 1239
Size: 393 367 229 1098 597 787 268 1524
Fragments arranged by size:
1524 1098 787 597 393 367 268 229
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 5263
EcoO109I rG'GnC_Cy
Cuts at: 2399 2530 2399
Size: 131 5132
EcoRI G'AATT_C
Cuts at: 2572 2950 2572
Size: 378 4885
FspI TGC'GCA
Cuts at: 2516 3769 2516
Size: 1253 4010
HaeII r_GCGC'y
Cuts at: 1678 2261 2272 4410 1678
Size: 583 11 2138 2531
Fragments arranged by size:
2531 2138 583 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3904 3936 3978 4010 1373
Size: 32 135 32 2332 32 42 32 2626
Fragments arranged by size:
2626 2332 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2607 2853 669
Size: 988 281 669 246 3079
Fragments arranged by size:
3079 988 669 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5263
HpaI GTT'AAC
Cuts at: 2853 2853
Size: 5263
KpnI G_GTAC'C
Cuts at: 2588 2588
Size: 5263
MfeI C'AATT_G
Cuts at: 2862 2862
Size: 5263
MluI A'CGCG_T
Cuts at: 5139 5139
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4377 4561 1305
Size: 3072 184 2007
Fragments arranged by size:
3072 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 2976
Fragments arranged by size:
2976 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3221 3580 3739 518
Size: 648 2055 359 159 2042
Fragments arranged by size:
2055 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2239 2679 3356 4504 4749 1606
Size: 633 440 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 440 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3772
Fragments arranged by size:
3772 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5263
NotI GC'GGCC_GC
Cuts at: 2710 2710
Size: 5263
NspI r_CATG'y
Cuts at: 1132 1229 2621 4166 1132
Size: 97 1392 1545 2229
Fragments arranged by size:
2229 1545 1392 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5263
PciI A'CATG_T
Cuts at: 4162 4162
Size: 5263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 2395 1108
Size: 1215 72 3976
Fragments arranged by size:
3976 1215 72
PmeI GTTT'AAAC
Cuts at: 2567 2567
Size: 5263
PmlI CAC'GTG
Cuts at: 2440 2440
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 4845 4877 3332
Size: 32 1481 32 3718
Fragments arranged by size:
3718 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2399 2399
Size: 5263
PshAI GACnn'nnGTC
Cuts at: 1613 2306 1613
Size: 693 4570
PsiI TTA'TAA
Cuts at: 2833 2833
Size: 5263
PspXI vC'TCGA_Gb
Cuts at: 2366 2366
Size: 5263
PstI C_TGCA'G
Cuts at: 830 2040 2615 830
Size: 1210 575 3478
Fragments arranged by size:
3478 1210 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5263
PvuII CAG'CTG
Cuts at: 2239 2239
Size: 5263
SalI G'TCGA_C
Cuts at: 1936 2605 1936
Size: 669 4594
SanDI GG'GwC_CC
Cuts at: 2399 2399
Size: 5263
SbfI CC_TGCA'GG
Cuts at: 2615 2615
Size: 5263
ScaI AGT'ACT
Cuts at: 3511 5223 3511
Size: 1712 3551
SexAI A'CCwGG_T
Cuts at: 2190 2319 2190
Size: 129 5134
SfcI C'TryA_G
Cuts at: 826 1045 2036 2611 3746 4427 4618 826
Size: 219 991 575 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 575 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 5263
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5263
SmaI CCC'GGG
Cuts at: 1872 2590 1872
Size: 718 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2366 3367 4268 4530 820
Size: 197 926 240 183 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 262 240 197 183
SnaBI TAC'GTA
Cuts at: 493 3054 493
Size: 2561 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5263
SphI G_CATG'C
Cuts at: 1132 2621 1132
Size: 1489 3774
SspI AAT'ATT
Cuts at: 5 52 3187 5
Size: 47 3135 2081
Fragments arranged by size:
3135 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5263
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2702 513
Size: 552 939 69 629 3074
Fragments arranged by size:
3074 939 629 552 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3290 3307 3460 3645 4090 325
Size: 1255 1710 17 153 185 445 1498
Fragments arranged by size:
1710 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3509 5221 96
Size: 274 80 33 51 1443 1532 1712 138
Fragments arranged by size:
1712 1532 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5223 5255 5223
Size: 32 5231
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5263
XbaI T'CTAG_A
Cuts at: 2599 2599
Size: 5263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2080
Size: 5263
XhoI C'TCGA_G
Cuts at: 1943 2366 1943
Size: 423 4840
XmaI C'CCGG_G
Cuts at: 1870 2588 1870
Size: 718 4545
XmnI GAAnn'nnTTC
Cuts at: 3392 3392
Size: 5263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3071 276
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII
SapI SfoI SrfI SwaI