Restriction map for
pFN21AB7066
(Circular) (Six-base) MAPSORT of: pFN21AB7066.seq Check: 4027 from: 1 to: 7150
pFN21AB7066 7150 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2379 3672 2379
Size: 1293 5857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4960 278
Size: 53 83 186 936 3424 2468
Fragments arranged by size:
3424 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4493 1937
Size: 2556 4594
Acc65I G'GTAC_C
Cuts at: 4471 4471
Size: 7150
AclI AA'CG_TT
Cuts at: 1563 5277 5650 1563
Size: 3714 373 3063
Fragments arranged by size:
3714 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3309 3848 4094 4148 4256 5213 6597 6865
Size: 1677 539 246 54 108 957 1384 268
Cuts at: 6865 1632
Size: 1917
Fragments arranged by size:
1917 1677 1384 957 539 268 246 108
54
AfeI AGC'GCT
Cuts at: 2278 2516 2278
Size: 238 6912
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6953
AflIII A'CryG_T
Cuts at: 1203 3564 6049 7026 1203
Size: 2361 2485 977 1327
Fragments arranged by size:
2485 2361 1327 977
AgeI A'CCGG_T
Cuts at: 3273 5981 3273
Size: 2708 4442
AhdI GACnn_n'nnGTC
Cuts at: 5879 5879
Size: 7150
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2960 1184
Size: 1776 5374
AlwNI CAG_nnn'CTG
Cuts at: 3448 3472 4893 6465 3448
Size: 24 1421 1572 4133
Fragments arranged by size:
4133 1572 1421 24
ApaI G_GGCC'C
Cuts at: 2859 3013 2859
Size: 154 6996
ApaLI G'TGCA_C
Cuts at: 5207 6363 5207
Size: 1156 5994
ApoI r'AATT_y
Cuts at: 1493 4459 4685 4837 6946 1493
Size: 2966 226 152 2109 1697
Fragments arranged by size:
2966 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5704 160
Size: 872 4672 1606
Fragments arranged by size:
4672 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
AvaI C'yCGr_G
Cuts at: 1870 1943 2143 2710 2910 3097 3874 4475 1870
Size: 73 200 567 200 187 777 601 4545
Fragments arranged by size:
4545 777 601 567 200 200 187 73
BamHI G'GATC_C
Cuts at: 4108 4480 4847 4108
Size: 372 367 6411
Fragments arranged by size:
6411 372 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4330 4471 5926 618
Size: 325 217 627 2543 141 1455 1842
Fragments arranged by size:
2543 1842 1455 627 325 217 141
BanII G_rGCy'C
Cuts at: 1391 2695 2703 2859 3013 3104 3919 4469 1391
Size: 1304 8 156 154 91 815 550 4072
Fragments arranged by size:
4072 1304 815 550 156 154 91 8
BbeI G_GCGC'C
Cuts at: 4334 4334
Size: 7150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3783 3867 4368 928
Size: 971 1884 84 501 3710
Fragments arranged by size:
3710 1884 971 501 84
BbvCI CC'TCA_GC
Cuts at: 2684 3019 2684
Size: 335 6815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2181 2215 2466 2500 5341 5375 1903
Size: 34 244 34 251 34 2841 34 3678
Fragments arranged by size:
3678 2841 251 244 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3957 5042 6252 3957
Size: 1085 1210 4855
Fragments arranged by size:
4855 1210 1085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7150
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 2495 2960
Size: 107 122 71 631 605 500 323 465
Cuts at: 2960 3692 4155 5761 136
Size: 732 463 1606 1525
Fragments arranged by size:
1606 1525 732 631 605 500 465 463
323 122 107 71
BglII A'GATC_T
Cuts at: 7145 7145
Size: 7150
BlpI GC'TnA_GC
Cuts at: 2589 4567 2589
Size: 1978 5172
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2859 3013 4210 5211 6367 1790
Size: 342 727 154 1197 1001 1156 2573
Fragments arranged by size:
2573 1197 1156 1001 727 342 154
BmgBI CAC'GTC
Cuts at: 1318 3534 1318
Size: 2216 4934
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3538 4881 5839
Size: 416 53 83 399 24 2121 1343 958
Cuts at: 5839 442
Size: 1753
Fragments arranged by size:
2121 1753 1343 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 3209 3823 4370 5810 1239
Size: 1307 663 614 547 1440 2579
Fragments arranged by size:
2579 1440 1307 663 614 547
Bpu10I CC'TnA_GC
Cuts at: 2684 3019 3398 4386 2684
Size: 335 379 988 5448
Fragments arranged by size:
5448 988 379 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4049 5275 6140 6438 4049
Size: 1226 865 298 4761
Fragments arranged by size:
4761 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5813 882
Size: 617 4314 2219
Fragments arranged by size:
4314 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3085 4941 493
Size: 711 1881 1856 2702
Fragments arranged by size:
2702 1881 1856 711
BsaBI GATnn'nnATC
Cuts at: 7144 7144
Size: 7150
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2573 4331 4957 5339
Size: 53 83 186 936 1040 1758 626 382
Cuts at: 5339 5974 275
Size: 635 1451
Fragments arranged by size:
1758 1451 1040 936 635 626 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3273 3988 5583 5981 6255 6402 6813 3273
Size: 715 1595 398 274 147 411 3610
Fragments arranged by size:
3610 1595 715 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2773 2803 2873 2903 3700
Size: 30 165 30 1370 30 70 30 797
Cuts at: 3700 3730 1178
Size: 30 4598
Fragments arranged by size:
4598 1370 797 165 70 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2781 4333 1186
Size: 913 682 1552 4003
Fragments arranged by size:
4003 1552 913 682
BseYI C'CCAG_C
Cuts at: 2105 2273 2511 2520 2730 3441 3637 4292 6353
Size: 168 238 9 210 711 196 655 2061
Cuts at: 6353 6849 2105
Size: 496 2406
Fragments arranged by size:
2406 2061 711 655 496 238 210 196
168 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2496 3668 3695 3828 1163
Size: 573 642 118 1172 27 133 4485
Fragments arranged by size:
4485 1172 642 573 133 118 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2490 2779 3486 4600 5361 6389
Size: 275 186 489 289 707 1114 761 1028
Cuts at: 6389 1540
Size: 2301
Fragments arranged by size:
2301 1114 1028 761 707 489 289 275
186
BsiHKAI G_wGCw'C
Cuts at: 3912 5211 5296 6367 3912
Size: 1299 85 1071 4695
Fragments arranged by size:
4695 1299 1071 85
BsiWI C'GTAC_G
Cuts at: 2319 3069 2319
Size: 750 6400
BsmI GAATG_Cn'
Cuts at: 2039 4661 4754 2039
Size: 2622 93 4435
Fragments arranged by size:
4435 2622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2695 2703 2859 3013 3104 3912
Size: 399 342 563 8 156 154 91 808
Cuts at: 3912 3919 4210 4469 5211 5296 6367 1391
Size: 7 291 259 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 563 399 342 291
259 156 154 91 85 8 7
BspEI T'CCGG_A
Cuts at: 3988 6813 3988
Size: 2825 4325
BspHI T'CATG_A
Cuts at: 3726 5039 6769 3726
Size: 1313 1730 4107
Fragments arranged by size:
4107 1730 1313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 3672 4505 844
Size: 1535 1293 833 3489
Fragments arranged by size:
3489 1535 1293 833
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 3402 5037 1394
Size: 429 200 1379 1635 3507
Fragments arranged by size:
3507 1635 1379 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 5645 5819 65
Size: 1183 4397 174 1396
Fragments arranged by size:
4397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2073 2188 2774 2940 2997 3273 3428
Size: 110 263 115 586 166 57 276 155
Cuts at: 3428 4431 5794 5981 1700
Size: 1003 1363 187 2869
Fragments arranged by size:
2869 1363 1003 586 276 263 187 166
155 115 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7150
BssSI C'ACGA_G
Cuts at: 5210 6222 5210
Size: 1012 6138
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2034 794
Size: 1240 5910
BstBI TT'CG_AA
Cuts at: 5954 5954
Size: 7150
BstEII G'GTnAC_C
Cuts at: 1184 3619 1184
Size: 2435 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2393 2411 2479 2545 3085 2257
Size: 136 18 68 66 540 6322
Fragments arranged by size:
6322 540 136 68 66 18
BstYI r'GATC_y
Cuts at: 1972 2058 3657 4108 4480 4847 5232 5249 6701
Size: 86 1599 451 372 367 385 17 1452
Cuts at: 6701 6787 6799 7145 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1599 1452 451 385 372 367 346
86 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3609 4404 3609
Size: 795 6355
BtgI C'CryG_G
Cuts at: 513 1065 2167 2295 3354 3642 3681 4104 4302
Size: 552 1102 128 1059 288 39 423 198
Cuts at: 4302 513
Size: 3361
Fragments arranged by size:
3361 1102 1059 552 423 288 198 128
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6411
Fragments arranged by size:
6411 683 56
BtsI GCAGTG_nn'
Cuts at: 2484 3561 4666 5460 5480 2484
Size: 1077 1105 794 20 4154
Fragments arranged by size:
4154 1105 1077 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2137 2172 529
Size: 35 1573 35 5507
Fragments arranged by size:
5507 1573 35 35
DraI TTT'AAA
Cuts at: 4454 4454
Size: 7150
DraIII CAC_nnn'GTG
Cuts at: 2122 3088 3320 3451 2122
Size: 966 232 131 5821
Fragments arranged by size:
5821 966 232 131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2347 6157 809
Size: 507 1031 3810 1802
Fragments arranged by size:
3810 1802 1031 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2298 2487
Size: 54 1425 50 275 18 328 140 189
Cuts at: 2487 2547 2776 2796 3268 3403 3522 3750 3882
Size: 60 229 20 472 135 119 228 132
Cuts at: 3882 4597 5486 7115 8
Size: 715 889 1629 43
Fragments arranged by size:
1629 1425 889 715 472 328 275 229
228 189 140 135 132 119 60 54
50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2487 2776 4597 1537
Size: 275 675 289 1821 4090
Fragments arranged by size:
4090 1821 675 289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2987 4310 4386 5080 1353
Size: 1634 1323 76 694 3423
Fragments arranged by size:
3423 1634 1323 694 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5737 6111 6257 387
Size: 1064 138 4148 374 146 1280
Fragments arranged by size:
4148 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 3209 3309 3823 3848 4094 4148
Size: 393 914 663 100 514 25 246 54
Cuts at: 4148 4256 4370 5213 5810 6597 6865 1239
Size: 108 114 843 597 787 268 1524
Fragments arranged by size:
1524 914 843 787 663 597 514 393
268 246 114 108 100 54 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3038 3038
Size: 7150
EcoO109I rG'GnC_Cy
Cuts at: 2092 2196 2340 2397 2715 2855 2856 2971 3009
Size: 104 144 57 318 140 1 115 38
Cuts at: 3009 3010 3613 2092
Size: 1 603 5629
Fragments arranged by size:
5629 603 318 144 140 115 104 57
38 1 1
EcoRI G'AATT_C
Cuts at: 4459 4837 4459
Size: 378 6772
FspI TGC'GCA
Cuts at: 5656 5656
Size: 7150
HaeII r_GCGC'y
Cuts at: 1678 2280 2518 4271 4334 6297 1678
Size: 602 238 1753 63 1963 2531
Fragments arranged by size:
2531 1963 1753 602 238 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3906 3938 5791 5823 5865
Size: 32 135 32 2334 32 1853 32 42
Cuts at: 5865 5897 1373
Size: 32 2626
Fragments arranged by size:
2626 2334 1853 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4494 4740 669
Size: 988 281 2556 246 3079
Fragments arranged by size:
3079 2556 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7150
HpaI GTT'AAC
Cuts at: 4740 4740
Size: 7150
KasI G'GCGC_C
Cuts at: 4330 4330
Size: 7150
KpnI G_GTAC'C
Cuts at: 4475 4475
Size: 7150
MfeI C'AATT_G
Cuts at: 4749 4749
Size: 7150
MluI A'CGCG_T
Cuts at: 7026 7026
Size: 7150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 6264 6448 1305
Size: 732 4227 184 2007
Fragments arranged by size:
4227 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 2549 3270 3752 10
Size: 54 1425 343 468 249 721 482 3408
Fragments arranged by size:
3408 1425 721 482 468 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 4170 5108 5467 5626 518
Size: 648 2331 673 938 359 159 2042
Fragments arranged by size:
2331 2042 938 673 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2443 2524 3182 4304 4566 5243 6391 6636
Size: 837 81 658 1122 262 677 1148 245
Cuts at: 6636 1606
Size: 2120
Fragments arranged by size:
2120 1148 1122 837 677 658 262 245
81
NaeI GCC'GGC
Cuts at: 2190 2942 2999 3430 2190
Size: 752 57 431 5910
Fragments arranged by size:
5910 752 431 57
NarI GG'CG_CC
Cuts at: 4331 4331
Size: 7150
NcoI C'CATG_G
Cuts at: 513 1065 3642 513
Size: 552 2577 4021
Fragments arranged by size:
4021 2577 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7150
NgoMIV G'CCGG_C
Cuts at: 2188 2940 2997 3428 2188
Size: 752 57 431 5910
Fragments arranged by size:
5910 752 431 57
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7150
NotI GC'GGCC_GC
Cuts at: 4597 4597
Size: 7150
NspI r_CATG'y
Cuts at: 1132 1229 4508 6053 1132
Size: 97 3279 1545 2229
Fragments arranged by size:
3279 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 5647
PciI A'CATG_T
Cuts at: 6049 6049
Size: 7150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2122 3448 4105 1108
Size: 1014 1326 657 4153
Fragments arranged by size:
4153 1326 1014 657
PmeI GTTT'AAAC
Cuts at: 4454 4454
Size: 7150
PmlI CAC'GTG
Cuts at: 3085 3085
Size: 7150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5219 5251 6732 6764 5219
Size: 32 1481 32 5605
Fragments arranged by size:
5605 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2196 2340 2397 2715 2971 2196
Size: 144 57 318 256 6375
Fragments arranged by size:
6375 318 256 144 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7150
PsiI TTA'TAA
Cuts at: 4720 4720
Size: 7150
PspOMI G'GGCC_C
Cuts at: 2855 3009 2855
Size: 154 6996
PstI C_TGCA'G
Cuts at: 830 2949 3556 4267 4502 830
Size: 2119 607 711 235 3478
Fragments arranged by size:
3478 2119 711 607 235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7150
PvuII CAG'CTG
Cuts at: 2443 2524 3182 2443
Size: 81 658 6411
Fragments arranged by size:
6411 658 81
SacII CC_GC'GG
Cuts at: 4305 4305
Size: 7150
SalI G'TCGA_C
Cuts at: 1936 4492 1936
Size: 2556 4594
SanDI GG'GwC_CC
Cuts at: 2340 2715 2340
Size: 375 6775
SapI GCTCTTCn'nnn_
Cuts at: 2987 2987
Size: 7150
SbfI CC_TGCA'GG
Cuts at: 4502 4502
Size: 7150
ScaI AGT'ACT
Cuts at: 5398 7110 5398
Size: 1712 5438
SexAI A'CCwGG_T
Cuts at: 3447 3447
Size: 7150
SfcI C'TryA_G
Cuts at: 826 1045 2945 3112 3552 4263 4498 5633 6314
Size: 219 1900 167 440 711 235 1135 681
Cuts at: 6314 6505 826
Size: 191 1471
Fragments arranged by size:
1900 1471 1135 711 681 440 235 219
191 167
SfiI GGCCn_nnn'nGGCC
Cuts at: 2495 2960 2495
Size: 465 6685
SfoI GGC'GCC
Cuts at: 4332 4332
Size: 7150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7150
SmaI CCC'GGG
Cuts at: 1872 2145 2712 3876 4477 1872
Size: 273 567 1164 601 4545
Fragments arranged by size:
4545 1164 601 567 273
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4064 5254 6155 6417 820
Size: 197 926 2121 1190 901 262 1553
Fragments arranged by size:
2121 1553 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4941 493
Size: 4448 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7150
SphI G_CATG'C
Cuts at: 1132 4508 1132
Size: 3376 3774
SrfI GCCC'GGGC
Cuts at: 3876 3876
Size: 7150
SspI AAT'ATT
Cuts at: 5 52 5074 5
Size: 47 5022 2081
Fragments arranged by size:
5022 2081 47
StuI AGG'CCT
Cuts at: 1517 2531 3218 1517
Size: 1014 687 5449
Fragments arranged by size:
5449 1014 687
StyI C'CwwG_G
Cuts at: 513 1065 2964 3642 4589 513
Size: 552 1899 678 947 3074
Fragments arranged by size:
3074 1899 947 678 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 5177 5194 5347 5532 5977 325
Size: 1255 2058 1539 17 153 185 445 1498
Fragments arranged by size:
2058 1539 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5396 7108 96
Size: 274 80 33 51 1443 3419 1712 138
Fragments arranged by size:
3419 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2773 2805 3568 3600 7110 7142 2430
Size: 32 311 32 763 32 3510 32 2438
Fragments arranged by size:
3510 2438 763 311 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7150
XbaI T'CTAG_A
Cuts at: 4486 4486
Size: 7150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3085 3745 3085
Size: 660 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7150
XmaI C'CCGG_G
Cuts at: 1870 2143 2710 3874 4475 1870
Size: 273 567 1164 601 4545
Fragments arranged by size:
4545 1164 601 567 273
XmnI GAAnn'nnTTC
Cuts at: 5279 5279
Size: 7150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4958 276
Size: 53 83 186 936 3424 2468
Fragments arranged by size:
3424 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SwaI