Restriction map for
pFN21AB7064
(Circular) (Six-base) MAPSORT of: pFN21AB7064.seq Check: 4800 from: 1 to: 6727
pFN21AB7064 6727 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4537 278
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4070 1937
Size: 2133 4594
Acc65I G'GTAC_C
Cuts at: 4048 4048
Size: 6727
AclI AA'CG_TT
Cuts at: 1563 4854 5227 1563
Size: 3291 373 3063
Fragments arranged by size:
3291 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 2274 2340 2532 2745 3041 3424 4790
Size: 630 12 66 192 213 296 383 1366
Cuts at: 4790 6174 6442 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1366 630 383 296 268 213
192 66 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6530
AflIII A'CryG_T
Cuts at: 1203 5626 6603 1203
Size: 4423 977 1327
Fragments arranged by size:
4423 1327 977
AgeI A'CCGG_T
Cuts at: 5558 5558
Size: 6727
AhdI GACnn_n'nnGTC
Cuts at: 2776 5456 2776
Size: 2680 4047
AleI CACnn'nnGTG
Cuts at: 1166 2663 1166
Size: 1497 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6727
AlwNI CAG_nnn'CTG
Cuts at: 2781 3447 3849 3998 4470 6042 2781
Size: 666 402 149 472 1572 3466
Fragments arranged by size:
3466 1572 666 472 402 149
ApaI G_GGCC'C
Cuts at: 3436 3746 3436
Size: 310 6417
ApaLI G'TGCA_C
Cuts at: 4784 5940 4784
Size: 1156 5571
ApoI r'AATT_y
Cuts at: 1493 4036 4262 4414 6523 1493
Size: 2543 226 152 2109 1697
Fragments arranged by size:
2543 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5281 160
Size: 872 4249 1606
Fragments arranged by size:
4249 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
AvaI C'yCGr_G
Cuts at: 1870 1943 3762 3837 4052 1870
Size: 73 1819 75 215 4545
Fragments arranged by size:
4545 1819 215 75 73
AvrII C'CTAG_G
Cuts at: 3578 3578
Size: 6727
BamHI G'GATC_C
Cuts at: 2645 4057 4424 2645
Size: 1412 367 4948
Fragments arranged by size:
4948 1412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2913 4048 5503 618
Size: 325 217 627 1126 1135 1455 1842
Fragments arranged by size:
1842 1455 1135 1126 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 3436 3516 3574 3746 3818 4046 1391
Size: 745 1300 80 58 172 72 228 4072
Fragments arranged by size:
4072 1300 745 228 172 80 72 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2262 2744 3148 928
Size: 971 363 482 404 4507
Fragments arranged by size:
4507 971 482 404 363
BbvCI CC'TCA_GC
Cuts at: 2090 2875 3123 3492 2090
Size: 785 248 369 5325
Fragments arranged by size:
5325 785 369 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3743 3760 3777 3794 4918 4952 1903
Size: 34 1806 17 17 17 1124 34 3678
Fragments arranged by size:
3678 1806 1124 34 34 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 4619 5829 4619
Size: 1210 5517
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 5338 136
Size: 107 122 71 631 605 443 3223 1525
Fragments arranged by size:
3223 1525 631 605 443 122 107 71
BglII A'GATC_T
Cuts at: 6722 6722
Size: 6727
BlpI GC'TnA_GC
Cuts at: 3510 3812 4144 3510
Size: 302 332 6093
Fragments arranged by size:
6093 332 302
Bme1580I G_kGCm'C
Cuts at: 1790 3436 3746 3856 4788 5944 1790
Size: 1646 310 110 932 1156 2573
Fragments arranged by size:
2573 1646 1156 932 310 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6727
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 2300 2850
Size: 416 53 83 399 24 652 231 550
Cuts at: 2850 2916 3508 3926 4458 5416 442
Size: 66 592 418 532 958 1753
Fragments arranged by size:
1753 958 652 592 550 532 418 416
399 231 83 66 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6727
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 6727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2204 2292 2625 3567 5387 1239
Size: 883 82 88 333 942 1820 2579
Fragments arranged by size:
2579 1820 942 883 333 88 82
Bpu10I CC'TnA_GC
Cuts at: 2090 2875 3123 3492 2090
Size: 785 248 369 5325
Fragments arranged by size:
5325 785 369 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3593 3970 4852 5717 6015 2885
Size: 708 377 882 865 298 3597
Fragments arranged by size:
3597 882 865 708 377 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5390 882
Size: 617 3891 2219
Fragments arranged by size:
3891 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4518 493
Size: 711 3314 2702
Fragments arranged by size:
3314 2702 711
BsaBI GATnn'nnATC
Cuts at: 6721 6721
Size: 6727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 2804 4534 4916
Size: 53 83 186 936 703 568 1730 382
Cuts at: 4916 5551 275
Size: 635 1451
Fragments arranged by size:
1730 1451 936 703 635 568 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3637 5160 5558 5832 5979 6390 3637
Size: 1523 398 274 147 411 3974
Fragments arranged by size:
3974 1523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6502
Fragments arranged by size:
6502 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2193 2261 2498 2531 2591 2594 2825
Size: 892 115 68 237 33 60 3 231
Cuts at: 2825 2948 3212 3215 3341 3344 3564 3821 3904
Size: 123 264 3 126 3 220 257 83
Cuts at: 3904 1186
Size: 4009
Fragments arranged by size:
4009 892 264 257 237 231 220 126
123 115 83 68 60 33 3 3
3
BseYI C'CCAG_C
Cuts at: 2435 2654 2881 2895 3447 5930 6426 2435
Size: 219 227 14 552 2483 496 2736
Fragments arranged by size:
2736 2483 552 496 227 219 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6154
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4177 4938 5966 1540
Size: 275 186 2176 761 1028 2301
Fragments arranged by size:
2301 2176 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2425 4788 4873 5944 2425
Size: 2363 85 1071 3208
Fragments arranged by size:
3208 2363 1071 85
BsmI GAATG_Cn'
Cuts at: 4238 4331 4238
Size: 93 6634
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2425 3436 3516 3574 3746 3818
Size: 399 346 289 1011 80 58 172 72
Cuts at: 3818 3856 4046 4788 4873 5944 1391
Size: 38 190 742 85 1071 2174
Fragments arranged by size:
2174 1071 1011 742 399 346 289 190
172 85 80 72 58 38
BspEI T'CCGG_A
Cuts at: 3637 6390 3637
Size: 2753 3974
BspHI T'CATG_A
Cuts at: 3631 4616 6346 3631
Size: 985 1730 4012
Fragments arranged by size:
4012 1730 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4082 844
Size: 3238 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 4614 1394
Size: 429 2155 636 3507
Fragments arranged by size:
3507 2155 636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5222 5396 65
Size: 1183 3974 174 1396
Fragments arranged by size:
3974 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3721 5371 5558 1700
Size: 110 1911 1650 187 2869
Fragments arranged by size:
2869 1911 1650 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6727
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6727
BssSI C'ACGA_G
Cuts at: 4787 5799 4787
Size: 1012 5715
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3998 794
Size: 3204 3523
BstBI TT'CG_AA
Cuts at: 5531 5531
Size: 6727
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6727
BstYI r'GATC_y
Cuts at: 1972 2645 4057 4424 4809 4826 6278 6364 6376
Size: 673 1412 367 385 17 1452 86 12
Cuts at: 6376 6722 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1412 673 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2111 3860 2111
Size: 1749 4978
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 2571 2697 513
Size: 552 939 35 532 126 4543
Fragments arranged by size:
4543 939 552 532 126 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5988
Fragments arranged by size:
5988 683 56
BtsI GCAGTG_nn'
Cuts at: 2453 4243 5037 5057 2453
Size: 1790 794 20 4123
Fragments arranged by size:
4123 1790 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6692
DraI TTT'AAA
Cuts at: 4031 4031
Size: 6727
DraIII CAC_nnn'GTG
Cuts at: 3011 3011
Size: 6727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2746 5734 809
Size: 507 1430 2988 1802
Fragments arranged by size:
2988 1802 1430 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 3038 3308
Size: 54 1425 50 275 18 583 625 270
Cuts at: 3308 3531 3963 4174 5063 6692 8
Size: 223 432 211 889 1629 43
Fragments arranged by size:
1629 1425 889 625 583 432 275 270
223 211 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4174 1537
Size: 275 2362 4090
Fragments arranged by size:
4090 2362 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2493 2508 2679 2925 3054 3105 3139
Size: 885 255 15 171 246 129 51 34
Cuts at: 3139 3474 4657 1353
Size: 335 1183 3423
Fragments arranged by size:
3423 1183 885 335 255 246 171 129
51 34 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5314 5688 5834 387
Size: 1064 138 3725 374 146 1280
Fragments arranged by size:
3725 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2204 2262 2274 2292 2340 2532
Size: 393 490 82 58 12 18 48 192
Cuts at: 2532 2625 2745 3041 3424 3567 4790 5387 6174
Size: 93 120 296 383 143 1223 597 787
Cuts at: 6174 6442 1239
Size: 268 1524
Fragments arranged by size:
1524 1223 787 597 490 393 383 296
268 192 143 120 93 82 58 48
18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3147 3147
Size: 6727
EcoO109I rG'GnC_Cy
Cuts at: 2116 2123 2267 2778 2892 3432 3742 3743 2116
Size: 7 144 511 114 540 310 1 5100
Fragments arranged by size:
5100 540 511 310 144 114 7 1
EcoRI G'AATT_C
Cuts at: 4036 4414 4036
Size: 378 6349
EcoRV GAT'ATC
Cuts at: 3455 3455
Size: 6727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3691 3691
Size: 6727
FseI GG_CCGG'CC
Cuts at: 3725 3725
Size: 6727
FspI TGC'GCA
Cuts at: 5233 5233
Size: 6727
HaeII r_GCGC'y
Cuts at: 1678 3616 5874 1678
Size: 1938 2258 2531
Fragments arranged by size:
2531 2258 1938
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 3077 3083 3109
Size: 32 135 32 1190 32 283 6 26
Cuts at: 3109 3115 3212 3244 3519 3551 5368 5400 5442
Size: 6 97 32 275 32 1817 32 42
Cuts at: 5442 5474 1373
Size: 32 2626
Fragments arranged by size:
2626 1817 1190 283 275 135 97 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 4071 4317 669
Size: 988 281 589 1544 246 3079
Fragments arranged by size:
3079 1544 988 589 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6727
HpaI GTT'AAC
Cuts at: 2527 4317 2527
Size: 1790 4937
KpnI G_GTAC'C
Cuts at: 4052 4052
Size: 6727
MfeI C'AATT_G
Cuts at: 4326 4326
Size: 6727
MluI A'CGCG_T
Cuts at: 6603 6603
Size: 6727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 3616 5841 6025 1305
Size: 1488 823 2225 184 2007
Fragments arranged by size:
2225 2007 1488 823 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 3040 3533 3965 10
Size: 54 1425 343 583 625 493 432 2772
Fragments arranged by size:
2772 1425 625 583 493 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 4685 5044 5203 518
Size: 648 1497 2022 359 159 2042
Fragments arranged by size:
2042 2022 1497 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2963 3995 4143 4820 5968 6213 1606
Size: 1357 1032 148 677 1148 245 2120
Fragments arranged by size:
2120 1357 1148 1032 677 245 148
NaeI GCC'GGC
Cuts at: 3723 3723
Size: 6727
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5236
Fragments arranged by size:
5236 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6727
NgoMIV G'CCGG_C
Cuts at: 3721 3721
Size: 6727
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6727
NotI GC'GGCC_GC
Cuts at: 4174 4174
Size: 6727
NspI r_CATG'y
Cuts at: 1132 1229 2356 4085 5630 1132
Size: 97 1127 1729 1545 2229
Fragments arranged by size:
2229 1729 1545 1127 97
PasI CC'CwG_GG
Cuts at: 1348 2477 2675 2861 3567 3747 1348
Size: 1129 198 186 706 180 4328
Fragments arranged by size:
4328 1129 706 198 186 180
PciI A'CATG_T
Cuts at: 5626 5626
Size: 6727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2661 2781 3703 3849 3961 1108
Size: 1553 120 922 146 112 3874
Fragments arranged by size:
3874 1553 922 146 120 112
PfoI T'CCnGG_A
Cuts at: 2641 2641
Size: 6727
PmeI GTTT'AAAC
Cuts at: 4031 4031
Size: 6727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 6309 6341 4796
Size: 32 1481 32 5182
Fragments arranged by size:
5182 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2123 2778 2123
Size: 655 6072
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6727
PsiI TTA'TAA
Cuts at: 4297 4297
Size: 6727
PspOMI G'GGCC_C
Cuts at: 3432 3742 3432
Size: 310 6417
PstI C_TGCA'G
Cuts at: 830 3994 4079 830
Size: 3164 85 3478
Fragments arranged by size:
3478 3164 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6727
PvuII CAG'CTG
Cuts at: 2963 2963
Size: 6727
SalI G'TCGA_C
Cuts at: 1936 4069 1936
Size: 2133 4594
SbfI CC_TGCA'GG
Cuts at: 4079 4079
Size: 6727
ScaI AGT'ACT
Cuts at: 4975 6687 4975
Size: 1712 5015
SfcI C'TryA_G
Cuts at: 826 1045 3147 3990 4075 5210 5891 6082 826
Size: 219 2102 843 85 1135 681 191 1471
Fragments arranged by size:
2102 1471 1135 843 681 219 191 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6727
SmaI CCC'GGG
Cuts at: 1872 3839 4054 1872
Size: 1967 215 4545
Fragments arranged by size:
4545 1967 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 3608 3949 4831 5732 5994
Size: 197 926 957 708 341 882 901 262
Cuts at: 5994 820
Size: 1553
Fragments arranged by size:
1553 957 926 901 882 708 341 262
197
SnaBI TAC'GTA
Cuts at: 493 4518 493
Size: 4025 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6727
SphI G_CATG'C
Cuts at: 1132 4085 1132
Size: 2953 3774
SrfI GCCC'GGGC
Cuts at: 3839 3839
Size: 6727
SspI AAT'ATT
Cuts at: 5 52 4651 5
Size: 47 4599 2081
Fragments arranged by size:
4599 2081 47
StuI AGG'CCT
Cuts at: 1517 3026 3296 1517
Size: 1509 270 4948
Fragments arranged by size:
4948 1509 270
StyI C'CwwG_G
Cuts at: 513 1065 2004 2225 3384 3578 4166 513
Size: 552 939 221 1159 194 588 3074
Fragments arranged by size:
3074 1159 939 588 552 221 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 3019 3289 3809 3911 4754 4771
Size: 1255 1073 366 270 520 102 843 17
Cuts at: 4771 4924 5109 5554 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1073 843 520 445 366 270
185 153 102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4973 6685 96
Size: 274 80 33 51 1443 2996 1712 138
Fragments arranged by size:
2996 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6687 6719 6687
Size: 32 6695
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6727
XbaI T'CTAG_A
Cuts at: 2128 4063 2128
Size: 1935 4792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3564 3564
Size: 6727
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6727
XmaI C'CCGG_G
Cuts at: 1870 3837 4052 1870
Size: 1967 215 4545
Fragments arranged by size:
4545 1967 215
XmnI GAAnn'nnTTC
Cuts at: 4856 4856
Size: 6727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4535 276
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SwaI