Restriction map for
pFN21AB7063
(Circular) (Six-base) MAPSORT of: pFN21AB7063.seq Check: 5868 from: 1 to: 6724
pFN21AB7063 6724 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 3251 4067 1937
Size: 846 468 816 4594
Fragments arranged by size:
4594 846 816 468
Acc65I G'GTAC_C
Cuts at: 4045 4045
Size: 6724
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3057 4787 6171 6439 1632
Size: 1425 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1425 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 3846 5623 6600 1203
Size: 2643 1777 977 1327
Fragments arranged by size:
2643 1777 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 2329 3877 5453 2329
Size: 1548 1576 3600
Fragments arranged by size:
3600 1576 1548
AleI CACnn'nnGTG
Cuts at: 1166 3500 1166
Size: 2334 4390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6724
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 2051
Size: 32 6692
AlwNI CAG_nnn'CTG
Cuts at: 2380 3421 4467 6039 2380
Size: 1041 1046 1572 3065
Fragments arranged by size:
3065 1572 1046 1041
ApaLI G'TGCA_C
Cuts at: 3088 4781 5937 3088
Size: 1693 1156 3875
Fragments arranged by size:
3875 1693 1156
ApoI r'AATT_y
Cuts at: 1493 3514 3658 4033 4259 4411 6520 1493
Size: 2021 144 375 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 375 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 2146 2177 2298 2327 3265 4049 1870
Size: 73 203 31 121 29 938 784 4545
Fragments arranged by size:
4545 938 784 203 121 73 31 29
BamHI G'GATC_C
Cuts at: 4054 4421 4054
Size: 367 6357
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2308 3125 4015 4045
Size: 325 217 627 227 294 817 890 30
Cuts at: 4045 5500 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 890 817 627 325 294 227
217 30
BanII G_rGCy'C
Cuts at: 1391 2076 2124 2135 2273 2435 2522 2699 3239
Size: 685 48 11 138 162 87 177 540
Cuts at: 3239 3266 3278 4043 1391
Size: 27 12 765 4072
Fragments arranged by size:
4072 765 685 540 177 162 138 87
48 27 12 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 928
Size: 971 303 5450
Fragments arranged by size:
5450 971 303
BbvCI CC'TCA_GC
Cuts at: 2391 2391
Size: 6724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3289 3323 4915 4949 1903
Size: 34 1352 34 1592 34 3678
Fragments arranged by size:
3678 1592 1352 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 2499 1548
Size: 951 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 5335 136
Size: 107 122 71 631 605 676 2987 1525
Fragments arranged by size:
2987 1525 676 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 6719 2550
Size: 4169 2555
BlpI GC'TnA_GC
Cuts at: 2360 3055 3990 4141 2360
Size: 695 935 151 4943
Fragments arranged by size:
4943 935 695 151
Bme1580I G_kGCm'C
Cuts at: 1790 2580 3092 4785 5941 1790
Size: 790 512 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 790 512
BmgBI CAC'GTC
Cuts at: 1318 2892 1318
Size: 1574 5150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4455 5413
Size: 416 53 83 399 24 1808 1230 958
Cuts at: 5413 442
Size: 1753
Fragments arranged by size:
1808 1753 1230 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3398 5384 1239
Size: 2159 1986 2579
Fragments arranged by size:
2579 2159 1986
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 6724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 3700 4849 5714 6012 2306
Size: 1394 1149 865 298 3018
Fragments arranged by size:
3018 1394 1149 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5387 882
Size: 617 3888 2219
Fragments arranged by size:
3888 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2100 4515 493
Size: 711 896 2415 2702
Fragments arranged by size:
2702 2415 896 711
BsaBI GATnn'nnATC
Cuts at: 6718 6718
Size: 6724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4531 4913 5548 275
Size: 53 83 186 936 2998 382 635 1451
Fragments arranged by size:
2998 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5157 5555 5829 5976 6387 5157
Size: 398 274 147 411 5494
Fragments arranged by size:
5494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2843 2873 1178
Size: 30 165 30 1440 30 5029
Fragments arranged by size:
5029 1440 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2198 3763 1186
Size: 1009 3 1565 4147
Fragments arranged by size:
4147 1565 1009 3
BseYI C'CCAG_C
Cuts at: 2266 2426 2862 3201 3567 3827 4011 5927 6423
Size: 160 436 339 366 260 184 1916 496
Cuts at: 6423 2266
Size: 2567
Fragments arranged by size:
2567 1916 496 436 366 339 260 184
160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2160 2241 4174 4935 5963 1540
Size: 275 186 159 81 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186 159 81
BsiHKAI G_wGCw'C
Cuts at: 2124 2522 2699 3092 3239 4785 4870 5941 2124
Size: 398 177 393 147 1546 85 1071 2907
Fragments arranged by size:
2907 1546 1071 398 393 177 147 85
BsmI GAATG_Cn'
Cuts at: 4235 4328 4235
Size: 93 6631
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 2147
Size: 6724
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2124 2135 2273 2435 2522 2580
Size: 399 286 48 11 138 162 87 58
Cuts at: 2580 2699 3092 3239 3266 3278 4043 4785 4870
Size: 119 393 147 27 12 765 742 85
Cuts at: 4870 5941 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 765 742 399 393 286 162
147 138 119 87 85 58 48 27
12 11
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 3456 4613 6343 3456
Size: 1157 1730 3837
Fragments arranged by size:
3837 1730 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3180 3429 3807 4079 844
Size: 2336 249 378 272 3489
Fragments arranged by size:
3489 2336 378 272 249
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2044 2259 2364 2733 4611 1394
Size: 429 212 9 215 105 369 1878 3507
Fragments arranged by size:
3507 1878 429 369 215 212 105 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 3547 5219 5393 65
Size: 1183 2299 1672 174 1396
Fragments arranged by size:
2299 1672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2222 2615 5368 5555 1700
Size: 110 207 205 393 2753 187 2869
Fragments arranged by size:
2869 2753 393 207 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 4784 5796 4784
Size: 1012 5712
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 3087 794
Size: 1607 686 4431
Fragments arranged by size:
4431 1607 686
BstBI TT'CG_AA
Cuts at: 5528 5528
Size: 6724
BstEII G'GTnAC_C
Cuts at: 1184 3401 1184
Size: 2217 4507
BstXI CCAn_nnnn'nTGG
Cuts at: 3835 3835
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2550 4054 4421 4806 4823 6275 6361 6373
Size: 578 1504 367 385 17 1452 86 12
Cuts at: 6373 6719 1972
Size: 346 1977
Fragments arranged by size:
1977 1504 1452 578 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2784 3252 2784
Size: 468 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2346 2763 2794 3104 513
Size: 552 939 228 114 417 31 310 4133
Fragments arranged by size:
4133 939 552 417 310 228 114 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3192 3465 487
Size: 683 56 1966 273 3746
Fragments arranged by size:
3746 1966 683 273 56
BtsI GCAGTG_nn'
Cuts at: 2648 2908 4240 5034 5054 2648
Size: 260 1332 794 20 4318
Fragments arranged by size:
4318 1332 794 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2776 2811 529
Size: 35 2212 35 4442
Fragments arranged by size:
4442 2212 35 35
DraI TTT'AAA
Cuts at: 4028 4028
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5731 809
Size: 507 4415 1802
Fragments arranged by size:
4415 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2797 2968
Size: 54 1425 50 275 18 327 640 171
Cuts at: 2968 4171 5060 6689 8
Size: 1203 889 1629 43
Fragments arranged by size:
1629 1425 1203 889 640 327 275 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2157 4171 1537
Size: 275 345 2014 4090
Fragments arranged by size:
4090 2014 345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2723 3388 4654 1353
Size: 1370 665 1266 3423
Fragments arranged by size:
3423 1370 1266 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2479 5311 5685 5831 387
Size: 1064 138 890 2832 374 146 1280
Fragments arranged by size:
2832 1280 1064 890 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2520 2697 3237 2122
Size: 398 177 540 5609
Fragments arranged by size:
5609 540 398 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3057 3398 4787 5384 6171 6439 1239
Size: 393 1425 341 1389 597 787 268 1524
Fragments arranged by size:
1524 1425 1389 787 597 393 341 268
EcoO109I rG'GnC_Cy
Cuts at: 3418 3418
Size: 6724
EcoRI G'AATT_C
Cuts at: 3514 3658 4033 4411 3514
Size: 144 375 378 5827
Fragments arranged by size:
5827 378 375 144
FspI TGC'GCA
Cuts at: 5230 5230
Size: 6724
HaeII r_GCGC'y
Cuts at: 1678 2381 3633 5871 1678
Size: 703 1252 2238 2531
Fragments arranged by size:
2531 2238 1252 703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2843 2875 3749
Size: 32 135 32 743 32 496 32 874
Cuts at: 3749 3781 3863 3895 5365 5397 5439 5471 1373
Size: 32 82 32 1470 32 42 32 2626
Fragments arranged by size:
2626 1470 874 743 496 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4068 4314 669
Size: 988 281 2130 246 3079
Fragments arranged by size:
3079 2130 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6724
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KpnI G_GTAC'C
Cuts at: 4049 4049
Size: 6724
MfeI C'AATT_G
Cuts at: 4323 4323
Size: 6724
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 3450 3897 5838 6022 1305
Size: 1677 468 447 1941 184 2007
Fragments arranged by size:
2007 1941 1677 468 447 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4902
Fragments arranged by size:
4902 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3450 3500 4682 5041 5200 518
Size: 648 2284 50 1182 359 159 2042
Fragments arranged by size:
2284 2042 1182 648 359 159 50
MspA1I CmG'CkG
Cuts at: 1606 2348 2401 2846 3232 3500 4140 4817 5965
Size: 742 53 445 386 268 640 677 1148
Cuts at: 5965 6210 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 742 677 640 445 386 268
245 53
NaeI GCC'GGC
Cuts at: 2224 2617 2224
Size: 393 6331
NcoI C'CATG_G
Cuts at: 513 1065 2004 2232 2763 513
Size: 552 939 228 531 4474
Fragments arranged by size:
4474 939 552 531 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NgoMIV G'CCGG_C
Cuts at: 2222 2615 2222
Size: 393 6331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 3850 4082 5627 1132
Size: 97 2621 232 1545 2229
Fragments arranged by size:
2621 2229 1545 232 97
PasI CC'CwG_GG
Cuts at: 1348 3625 1348
Size: 2277 4447
PciI A'CATG_T
Cuts at: 3846 5623 3846
Size: 1777 4947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6724
PfoI T'CCnGG_A
Cuts at: 3012 3414 3012
Size: 402 6322
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2469 4793 4825 6306 6338 2437
Size: 32 2324 32 1481 32 2823
Fragments arranged by size:
2823 2324 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3418 3418
Size: 6724
PshAI GACnn'nnGTC
Cuts at: 1613 2853 1613
Size: 1240 5484
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PstI C_TGCA'G
Cuts at: 830 2388 2829 4076 830
Size: 1558 441 1247 3478
Fragments arranged by size:
3478 1558 1247 441
PvuI CG_AT'CG
Cuts at: 2001 2241 2001
Size: 240 6484
PvuII CAG'CTG
Cuts at: 2846 2846
Size: 6724
SacI G_AGCT'C
Cuts at: 2124 2522 2699 3239 2124
Size: 398 177 540 5609
Fragments arranged by size:
5609 540 398 177
SacII CC_GC'GG
Cuts at: 2349 2349
Size: 6724
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SapI GCTCTTCn'nnn_
Cuts at: 2723 2723
Size: 6724
SbfI CC_TGCA'GG
Cuts at: 4076 4076
Size: 6724
ScaI AGT'ACT
Cuts at: 4972 6684 4972
Size: 1712 5012
SfcI C'TryA_G
Cuts at: 826 1045 2384 2825 3046 4072 5207 5888 6079
Size: 219 1339 441 221 1026 1135 681 191
Cuts at: 6079 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 1026 681 441 221 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 2300 2329 4051 1872
Size: 428 29 1722 4545
Fragments arranged by size:
4545 1722 428 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 3679 4828 5729 5991 820
Size: 197 926 342 1394 1149 901 262 1553
Fragments arranged by size:
1553 1394 1149 926 901 342 262 197
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 3788 152
Size: 3636 3088
SphI G_CATG'C
Cuts at: 1132 4082 1132
Size: 2950 3774
SspI AAT'ATT
Cuts at: 5 52 4648 5
Size: 47 4596 2081
Fragments arranged by size:
4596 2081 47
StuI AGG'CCT
Cuts at: 1517 3998 1517
Size: 2481 4243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2232 2763 3258 3697 4163 513
Size: 552 939 228 531 495 439 466 3074
Fragments arranged by size:
3074 939 552 531 495 466 439 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4003 4751 4768 4921 5106 5551 325
Size: 1255 2423 748 17 153 185 445 1498
Fragments arranged by size:
2423 1498 1255 748 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3780 4970 6682
Size: 274 80 33 51 1443 1803 1190 1712
Cuts at: 6682 96
Size: 138
Fragments arranged by size:
1803 1712 1443 1190 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6684 6716 6684
Size: 32 6692
Tth111I GACn'n_nGTC
Cuts at: 1316 3466 1316
Size: 2150 4574
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3632 3632
Size: 6724
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 2298 2327 4049 1870
Size: 428 29 1722 4545
Fragments arranged by size:
4545 1722 428 29
XmnI GAAnn'nnTTC
Cuts at: 4853 4853
Size: 6724
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI