Restriction map for
pFN21AB7062
(Circular) (Six-base) MAPSORT of: pFN21AB7062.seq Check: 1399 from: 1 to: 6697
pFN21AB7062 6697 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 2023
Size: 6697
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4507 278
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3166 3387 3771 4040 1937
Size: 1229 221 384 269 4594
Fragments arranged by size:
4594 1229 384 269 221
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 2618 2996 3993 4760 6144 6412 1632
Size: 630 356 378 997 767 1384 268 1917
Fragments arranged by size:
1917 1384 997 767 630 378 356 268
AflII C'TTAA_G
Cuts at: 820 1017 2773 3058 820
Size: 197 1756 285 4459
Fragments arranged by size:
4459 1756 285 197
AflIII A'CryG_T
Cuts at: 1203 2244 2334 2646 3069 5596 6573 1203
Size: 1041 90 312 423 2527 977 1327
Fragments arranged by size:
2527 1327 1041 977 423 312 90
AgeI A'CCGG_T
Cuts at: 5528 5528
Size: 6697
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3261 1184
Size: 2077 4620
AlwNI CAG_nnn'CTG
Cuts at: 3122 3971 4440 6012 3122
Size: 849 469 1572 3807
Fragments arranged by size:
3807 1572 849 469
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 6697
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 2655 3419 3486 3977 4006 4232 4384 6493
Size: 1162 764 67 491 29 226 152 2109
Cuts at: 6493 1493
Size: 1697
Fragments arranged by size:
2109 1697 1162 764 491 226 152 67
29
AseI AT'TA_AT
Cuts at: 160 1032 2465 3128 3591 5251 160
Size: 872 1433 663 463 1660 1606
Fragments arranged by size:
1660 1606 1433 872 663 463
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 4022 1870
Size: 73 2079 4545
Fragments arranged by size:
4545 2079 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3981 4014 3981
Size: 33 6664
BamHI G'GATC_C
Cuts at: 4027 4394 4027
Size: 367 6330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2324 4018 5473 618
Size: 325 217 627 299 238 1694 1455 1842
Fragments arranged by size:
1842 1694 1455 627 325 299 238 217
BanII G_rGCy'C
Cuts at: 1391 2022 4016 1391
Size: 631 1994 4072
Fragments arranged by size:
4072 1994 631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3560 928
Size: 971 1661 4065
Fragments arranged by size:
4065 1661 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2633 2667 4888 4922 1903
Size: 34 696 34 2221 34 3678
Fragments arranged by size:
3678 2221 696 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2417 4589 5799 2417
Size: 2172 1210 3315
Fragments arranged by size:
3315 2172 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6697
BfrBI ATG'CAT
Cuts at: 3515 3515
Size: 6697
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5308 136
Size: 107 122 71 631 605 337 3299 1525
Fragments arranged by size:
3299 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2166 6692 2166
Size: 4526 2171
BlpI GC'TnA_GC
Cuts at: 2254 4114 2254
Size: 1860 4837
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2091 4758 5914 1790
Size: 232 69 2667 1156 2573
Fragments arranged by size:
2667 2573 1156 232 69
BmgBI CAC'GTC
Cuts at: 1318 2337 1318
Size: 1019 5678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2888 3027 4428
Size: 416 53 83 399 24 1471 139 1401
Cuts at: 4428 5386 442
Size: 958 1753
Fragments arranged by size:
1753 1471 1401 958 416 399 139 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2423 3828 5357 1239
Size: 908 276 1405 1529 2579
Fragments arranged by size:
2579 1529 1405 908 276
Bpu10I CC'TnA_GC
Cuts at: 2748 2748
Size: 6697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4822 5687 5985 4822
Size: 865 298 5534
Fragments arranged by size:
5534 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5360 882
Size: 617 3861 2219
Fragments arranged by size:
3861 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2247 4488 493
Size: 711 1043 2241 2702
Fragments arranged by size:
2702 2241 1043 711
BsaBI GATnn'nnATC
Cuts at: 6691 6691
Size: 6697
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 2968 4504 4886
Size: 53 83 186 936 576 859 1536 382
Cuts at: 4886 5521 275
Size: 635 1451
Fragments arranged by size:
1536 1451 936 859 635 576 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5130 5528 5802 5949 6360 5130
Size: 398 274 147 411 5467
Fragments arranged by size:
5467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6472
Fragments arranged by size:
6472 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6697
BseYI C'CCAG_C
Cuts at: 5900 6396 5900
Size: 496 6201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4147 4908 5936 1540
Size: 275 186 12 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2163 4758 4843 5914 2163
Size: 2595 85 1071 2946
Fragments arranged by size:
2946 2595 1071 85
BsmI GAATG_Cn'
Cuts at: 4208 4301 4208
Size: 93 6604
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2091 2163 4016 4758 4843 5914
Size: 399 232 69 72 1853 742 85 1071
Cuts at: 5914 1391
Size: 2174
Fragments arranged by size:
2174 1853 1071 742 399 232 85 72
69
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 3336 3658 4586 6316 3336
Size: 322 928 1730 3717
Fragments arranged by size:
3717 1730 928 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2044 4052 844
Size: 1179 21 2008 3489
Fragments arranged by size:
3489 2008 1179 21
BsrBI CCG'CTC
Cuts at: 1394 1823 4584 1394
Size: 429 2761 3507
Fragments arranged by size:
3507 2761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5192 5366 65
Size: 1183 3944 174 1396
Fragments arranged by size:
3944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 5341 5528 1700
Size: 110 335 3196 187 2869
Fragments arranged by size:
3196 2869 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 2766 3732 96
Size: 2670 966 3061
Fragments arranged by size:
3061 2670 966
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6697
BssSI C'ACGA_G
Cuts at: 4757 5769 4757
Size: 1012 5685
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 794
Size: 1241 5456
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6697
BstYI r'GATC_y
Cuts at: 1972 2166 2476 4027 4394 4779 4796 6248 6334
Size: 194 310 1551 367 385 17 1452 86
Cuts at: 6334 6346 6692 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1551 1452 385 367 346 310 194
86 17 12
BstZ17I GTA'TAC
Cuts at: 3772 3772
Size: 6697
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 3106 513
Size: 552 939 9 1093 4104
Fragments arranged by size:
4104 1093 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5958
Fragments arranged by size:
5958 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 4213 5007 5027 2595
Size: 1618 794 20 4265
Fragments arranged by size:
4265 1618 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6662
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3383 5704 809
Size: 507 2067 2321 1802
Fragments arranged by size:
2321 2067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 4144 5033
Size: 54 1425 50 275 18 180 2134 889
Cuts at: 5033 6662 8
Size: 1629 43
Fragments arranged by size:
2134 1629 1425 889 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 4144 1537
Size: 275 198 2134 4090
Fragments arranged by size:
4090 2134 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2687 3015 3036 4627 1353
Size: 1334 328 21 1591 3423
Fragments arranged by size:
3423 1591 1334 328 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5284 5658 5804 387
Size: 1064 138 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2262 2423 2618 2996 3828 3993
Size: 393 515 115 161 195 378 832 165
Cuts at: 3993 4760 5357 6144 6412 1239
Size: 767 597 787 268 1524
Fragments arranged by size:
1524 832 787 767 597 515 393 378
268 195 165 161 115
EcoNI CCTnn'n_nnAGG
Cuts at: 2367 2493 2367
Size: 126 6571
EcoO109I rG'GnC_Cy
Cuts at: 2018 2019 2372 2827 2018
Size: 1 353 455 5888
Fragments arranged by size:
5888 455 353 1
EcoRI G'AATT_C
Cuts at: 4006 4384 4006
Size: 378 6319
EcoRV GAT'ATC
Cuts at: 3787 3787
Size: 6697
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3065 3065
Size: 6697
FspI TGC'GCA
Cuts at: 5203 5203
Size: 6697
HaeII r_GCGC'y
Cuts at: 1678 2069 2081 5844 1678
Size: 391 12 3763 2531
Fragments arranged by size:
3763 2531 391 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 5338 5370 5412
Size: 32 135 32 460 32 3274 32 42
Cuts at: 5412 5444 1373
Size: 32 2626
Fragments arranged by size:
3274 2626 460 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3371 748
Size: 2623 4074
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5811 5995 1305
Size: 4506 184 2007
Fragments arranged by size:
4506 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4875
Fragments arranged by size:
4875 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3243 3464 4655 5014 5173 518
Size: 648 2077 221 1191 359 159 2042
Fragments arranged by size:
2077 2042 1191 648 359 221 159
MspA1I CmG'CkG
Cuts at: 1606 3971 4113 4790 5938 6183 1606
Size: 2365 142 677 1148 245 2120
Fragments arranged by size:
2365 2120 1148 677 245 142
NcoI C'CATG_G
Cuts at: 513 1065 2004 3106 513
Size: 552 939 1102 4104
Fragments arranged by size:
4104 1102 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NsiI A_TGCA'T
Cuts at: 3517 3517
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 2650 3073 3515 4055 5600 1132
Size: 97 1421 423 442 540 1545 2229
Fragments arranged by size:
2229 1545 1421 540 442 423 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 2646 3069 5596 2646
Size: 423 2527 3747
Fragments arranged by size:
3747 2527 423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2917 1108
Size: 1809 4888
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 6279 6311 4766
Size: 32 1481 32 5152
Fragments arranged by size:
5152 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2372 2827 2372
Size: 455 6242
PshAI GACnn'nnGTC
Cuts at: 1613 2967 1613
Size: 1354 5343
PsiI TTA'TAA
Cuts at: 2846 4267 2846
Size: 1421 5276
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 6697
PstI C_TGCA'G
Cuts at: 830 3091 4049 830
Size: 2261 958 3478
Fragments arranged by size:
3478 2261 958
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 3971 3971
Size: 6697
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SapI GCTCTTCn'nnn_
Cuts at: 3015 3015
Size: 6697
SbfI CC_TGCA'GG
Cuts at: 4049 4049
Size: 6697
ScaI AGT'ACT
Cuts at: 4945 6657 4945
Size: 1712 4985
SfcI C'TryA_G
Cuts at: 826 1045 2402 2754 3087 4045 5180 5861 6052
Size: 219 1357 352 333 958 1135 681 191
Cuts at: 6052 826
Size: 1471
Fragments arranged by size:
1471 1357 1135 958 681 352 333 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2773 3058 4801 5702 5964 820
Size: 197 926 830 285 1743 901 262 1553
Fragments arranged by size:
1743 1553 926 901 830 285 262 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 4055 1132
Size: 2923 3774
SspI AAT'ATT
Cuts at: 5 52 2931 4621 5
Size: 47 2879 1690 2081
Fragments arranged by size:
2879 2081 1690 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6697
StyI C'CwwG_G
Cuts at: 513 1065 2004 3106 4136 513
Size: 552 939 1102 1030 3074
Fragments arranged by size:
3074 1102 1030 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2844 3847 4724 4741 4894 5079 5524
Size: 1255 1264 1003 877 17 153 185 445
Cuts at: 5524 325
Size: 1498
Fragments arranged by size:
1498 1264 1255 1003 877 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2766 2875 3732
Size: 274 80 33 51 1443 789 109 857
Cuts at: 3732 3905 4943 6655 96
Size: 173 1038 1712 138
Fragments arranged by size:
1712 1443 1038 857 789 274 173 138
109 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6657 6689 6657
Size: 32 6665
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 4033 4033
Size: 6697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6697
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 4826 4826
Size: 6697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4505 276
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI
BsmBI BstXI Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI