Restriction map for
pFN21AB7058
(Circular) (Six-base) MAPSORT of: pFN21AB7058.seq Check: 4576 from: 1 to: 6544
pFN21AB7058 6544 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3392 3392
Size: 6544
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3887 1937
Size: 1950 4594
Acc65I G'GTAC_C
Cuts at: 3865 3865
Size: 6544
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 2765 3655 4607 5991 6259 1632
Size: 794 339 890 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 890 794 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 5443 6420 1203
Size: 4240 977 1327
Fragments arranged by size:
4240 1327 977
AgeI A'CCGG_T
Cuts at: 5375 5375
Size: 6544
AhdI GACnn_n'nnGTC
Cuts at: 5273 5273
Size: 6544
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3029 3274 1184
Size: 1845 245 4454
Fragments arranged by size:
4454 1845 245
AlwNI CAG_nnn'CTG
Cuts at: 4287 5859 4287
Size: 1572 4972
ApaLI G'TGCA_C
Cuts at: 2136 4601 5757 2136
Size: 2465 1156 2923
Fragments arranged by size:
2923 2465 1156
ApoI r'AATT_y
Cuts at: 1493 2185 3853 4079 4231 6340 1493
Size: 692 1668 226 152 2109 1697
Fragments arranged by size:
2109 1697 1668 692 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2087 2211 2278 2332 2379 2647
Size: 73 71 73 124 67 54 47 268
Cuts at: 2647 3435 3869 1870
Size: 788 434 4545
Fragments arranged by size:
4545 788 434 268 124 73 73 71
67 54 47
BamHI G'GATC_C
Cuts at: 2672 3874 4241 2672
Size: 1202 367 4975
Fragments arranged by size:
4975 1202 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3865 5320 618
Size: 325 217 627 2078 1455 1842
Fragments arranged by size:
2078 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2014 2212 3175 3247 3863 1391
Size: 623 198 963 72 616 4072
Fragments arranged by size:
4072 963 623 616 198 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 3720 928
Size: 971 623 1198 3752
Fragments arranged by size:
3752 1198 971 623
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4735 4769 1903
Size: 34 50 34 2714 34 3678
Fragments arranged by size:
3678 2714 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4436 5646 4436
Size: 1210 5334
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6544
BfrBI ATG'CAT
Cuts at: 3717 3717
Size: 6544
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2912 3557 5155
Size: 107 122 71 631 605 1240 645 1598
Cuts at: 5155 136
Size: 1525
Fragments arranged by size:
1598 1525 1240 645 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2445 3270 6539 2445
Size: 825 3269 2450
Fragments arranged by size:
3269 2450 825
BlpI GC'TnA_GC
Cuts at: 3212 3961 3212
Size: 749 5795
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2625 3018 4605 5761 1790
Size: 350 485 393 1587 1156 2573
Fragments arranged by size:
2573 1587 1156 485 393 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3635 4275 5233
Size: 416 53 83 399 24 2218 640 958
Cuts at: 5233 442
Size: 1753
Fragments arranged by size:
2218 1753 958 640 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 6544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2383 2414 2555 3011 5204 1239
Size: 1144 31 141 456 2193 2579
Fragments arranged by size:
2579 2193 1144 456 141 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 4669 5534 5832 2048
Size: 2621 865 298 2760
Fragments arranged by size:
2760 2621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5207 882
Size: 617 3708 2219
Fragments arranged by size:
3708 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4335 493
Size: 711 3131 2702
Fragments arranged by size:
3131 2702 711
BsaBI GATnn'nnATC
Cuts at: 2855 6538 2855
Size: 3683 2861
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2315 4351 4733
Size: 53 83 186 936 636 146 2036 382
Cuts at: 4733 5368 275
Size: 635 1451
Fragments arranged by size:
2036 1451 936 636 635 382 186 146
83 53
BsaWI w'CCGG_w
Cuts at: 4977 5375 5649 5796 6207 4977
Size: 398 274 147 411 5314
Fragments arranged by size:
5314 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6319
Fragments arranged by size:
6319 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2468 1186
Size: 1054 228 5262
Fragments arranged by size:
5262 1054 228
BseYI C'CCAG_C
Cuts at: 5747 6243 5747
Size: 496 6048
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5971
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3994 4755 5783 1540
Size: 275 186 1993 761 1028 2301
Fragments arranged by size:
2301 1993 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 2218 2380 3067 3175 3247 4605 4690 5761
Size: 78 162 687 108 72 1358 85 1071
Cuts at: 5761 2140
Size: 2923
Fragments arranged by size:
2923 1358 1071 687 162 108 85 78
72
BsmI GAATG_Cn'
Cuts at: 3717 3719 4055 4148 3717
Size: 2 336 93 6113
Fragments arranged by size:
6113 336 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 2190 2190
Size: 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2140 2212 2218 2380 2625 3018
Size: 399 224 126 72 6 162 245 393
Cuts at: 3018 3067 3175 3247 3863 4605 4690 5761 1391
Size: 49 108 72 616 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 393 245 224
162 126 108 85 72 72 49 6
BspEI T'CCGG_A
Cuts at: 6207 6207
Size: 6544
BspHI T'CATG_A
Cuts at: 4433 6163 4433
Size: 1730 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 3392 3899 844
Size: 1430 1118 507 3489
Fragments arranged by size:
3489 1430 1118 507
BsrBI CCG'CTC
Cuts at: 1394 1823 4431 1394
Size: 429 2608 3507
Fragments arranged by size:
3507 2608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3838 5039 5213 65
Size: 1183 2590 1201 174 1396
Fragments arranged by size:
2590 1396 1201 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 2730 2825 5188 5375 1700
Size: 110 459 461 95 2363 187 2869
Fragments arranged by size:
2869 2363 461 459 187 110 95
BsrGI T'GTAC_A
Cuts at: 96 3479 96
Size: 3383 3161
BssHII G'CGCG_C
Cuts at: 1925 2019 1925
Size: 94 6450
BssSI C'ACGA_G
Cuts at: 2966 4604 5616 2966
Size: 1638 1012 3894
Fragments arranged by size:
3894 1638 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2862 794
Size: 2068 4476
BstBI TT'CG_AA
Cuts at: 5348 5348
Size: 6544
BstEII G'GTnAC_C
Cuts at: 1184 3736 1184
Size: 2552 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 2614 2699 2614
Size: 85 6459
BstYI r'GATC_y
Cuts at: 1972 2445 2672 3270 3874 4241 4626 4643 6095
Size: 473 227 598 604 367 385 17 1452
Cuts at: 6095 6181 6193 6539 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 604 598 473 385 367 346
227 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2158 2421 2808 3768 513
Size: 552 1093 263 387 960 3289
Fragments arranged by size:
3289 1093 960 552 387 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5805
Fragments arranged by size:
5805 683 56
BtsI GCAGTG_nn'
Cuts at: 4060 4854 4874 4060
Size: 794 20 5730
Fragments arranged by size:
5730 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6509
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2773 5551 809
Size: 507 1457 2778 1802
Fragments arranged by size:
2778 1802 1457 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2549 2604 2904
Size: 54 1425 50 275 18 719 55 300
Cuts at: 2904 3238 3991 4880 6509 8
Size: 334 753 889 1629 43
Fragments arranged by size:
1629 1425 889 753 719 334 300 275
55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3991 1537
Size: 275 2179 4090
Fragments arranged by size:
4090 2179 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 2441 2454 4474 1353
Size: 730 358 13 2020 3423
Fragments arranged by size:
3423 2020 730 358 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2324 3194 5131 5505 5651 387
Size: 1064 138 735 870 1937 374 146 1280
Fragments arranged by size:
1937 1280 1064 870 735 374 146 138
EcoICRI GAG'CTC
Cuts at: 3173 3245 3173
Size: 72 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2383 2414 2426 2555 2765 3011 3655
Size: 393 751 31 12 129 210 246 644
Cuts at: 3655 4607 5204 5991 6259 1239
Size: 952 597 787 268 1524
Fragments arranged by size:
1524 952 787 751 644 597 393 268
246 210 129 31 12
EcoO109I rG'GnC_Cy
Cuts at: 2252 2263 2336 2468 2705 2252
Size: 11 73 132 237 6091
Fragments arranged by size:
6091 237 132 73 11
EcoRI G'AATT_C
Cuts at: 3853 4231 3853
Size: 378 6166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 6544
FspI TGC'GCA
Cuts at: 5050 5050
Size: 6544
HaeII r_GCGC'y
Cuts at: 1678 2158 2481 2660 5691 1678
Size: 480 323 179 3031 2531
Fragments arranged by size:
3031 2531 480 323 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5185 5217 5259 5291 1373
Size: 32 135 32 3613 32 42 32 2626
Fragments arranged by size:
3613 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3888 4134 669
Size: 988 281 1950 246 3079
Fragments arranged by size:
3079 1950 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6544
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KpnI G_GTAC'C
Cuts at: 3869 3869
Size: 6544
MfeI C'AATT_G
Cuts at: 2637 4143 2637
Size: 1506 5038
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2366 2916 3811 5658 5842 1305
Size: 1061 550 895 1847 184 2007
Fragments arranged by size:
2007 1847 1061 895 550 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2551 2906 10
Size: 54 1425 343 719 355 3648
Fragments arranged by size:
3648 1425 719 355 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4502 4861 5020 518
Size: 648 3336 359 159 2042
Fragments arranged by size:
3336 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2764 2953 3211 3960 4637 5785 6030
Size: 554 604 189 258 749 677 1148 245
Cuts at: 6030 1606
Size: 2120
Fragments arranged by size:
2120 1148 749 677 604 554 258 245
189
NaeI GCC'GGC
Cuts at: 2271 2271
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5992
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NgoMIV G'CCGG_C
Cuts at: 2269 2269
Size: 6544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6544
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NsiI A_TGCA'T
Cuts at: 3719 3719
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 2038 2881 3902 5447 1132
Size: 97 809 843 1021 1545 2229
Fragments arranged by size:
2229 1545 1021 843 809 97
PasI CC'CwG_GG
Cuts at: 1348 2220 1348
Size: 872 5672
PciI A'CATG_T
Cuts at: 5443 5443
Size: 6544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2982 1108
Size: 1874 4670
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3765 3797 4613 4645 6126 6158 3765
Size: 32 816 32 1481 32 4151
Fragments arranged by size:
4151 1481 816 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2252 2263 2336 2468 2705 2252
Size: 11 73 132 237 6091
Fragments arranged by size:
6091 237 132 73 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6544
PsiI TTA'TAA
Cuts at: 4114 4114
Size: 6544
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 6512
PstI C_TGCA'G
Cuts at: 830 2299 3896 830
Size: 1469 1597 3478
Fragments arranged by size:
3478 1597 1469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6544
PvuII CAG'CTG
Cuts at: 3211 3211
Size: 6544
SacI G_AGCT'C
Cuts at: 3175 3247 3175
Size: 72 6472
SacII CC_GC'GG
Cuts at: 2161 2161
Size: 6544
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SanDI GG'GwC_CC
Cuts at: 2252 2468 2705 2252
Size: 216 237 6091
Fragments arranged by size:
6091 237 216
SapI GCTCTTCn'nnn_
Cuts at: 2441 2441
Size: 6544
SbfI CC_TGCA'GG
Cuts at: 3896 3896
Size: 6544
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SfcI C'TryA_G
Cuts at: 826 1045 2142 2295 3561 3892 5027 5708 5899
Size: 219 1097 153 1266 331 1135 681 191
Cuts at: 5899 826
Size: 1471
Fragments arranged by size:
1471 1266 1135 1097 681 331 219 191
153
SfiI GGCCn_nnn'nGGCC
Cuts at: 2912 2912
Size: 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 2016 2280 2334 2649 3871 1872
Size: 144 264 54 315 1222 4545
Fragments arranged by size:
4545 1222 315 264 144 54
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 4648 5549 5811 820
Size: 197 926 120 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3902 1132
Size: 2770 3774
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6544
StyI C'CwwG_G
Cuts at: 513 1065 2552 2676 3983 513
Size: 552 1487 124 1307 3074
Fragments arranged by size:
3074 1487 1307 552 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 2669 3154 4571 4588 4741 4926
Size: 1255 653 436 485 1417 17 153 185
Cuts at: 4926 5371 325
Size: 445 1498
Fragments arranged by size:
1498 1417 1255 653 485 445 436 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3108 3479 3762
Size: 274 80 33 51 1443 1131 371 283
Cuts at: 3762 4790 6502 96
Size: 1028 1712 138
Fragments arranged by size:
1712 1443 1131 1028 371 283 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6504 6536 6504
Size: 32 6512
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6544
XbaI T'CTAG_A
Cuts at: 3880 3880
Size: 6544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 2777 2899 2358
Size: 419 122 6003
Fragments arranged by size:
6003 419 122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6544
XmaI C'CCGG_G
Cuts at: 1870 2014 2278 2332 2647 3869 1870
Size: 144 264 54 315 1222 4545
Fragments arranged by size:
4545 1222 315 264 144 54
XmnI GAAnn'nnTTC
Cuts at: 2753 3198 3717 4673 2753
Size: 445 519 956 4624
Fragments arranged by size:
4624 956 519 445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI RsrII SexAI SfoI SrfI SwaI