Restriction map for
pFN21AB7057
(Circular) (Six-base) MAPSORT of: pFN21AB7057.seq Check: 967 from: 1 to: 6520
pFN21AB7057 6520 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 3133 3357 2391
Size: 742 224 5554
Fragments arranged by size:
5554 742 224
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3471 4330 278
Size: 53 83 186 936 1935 859 2468
Fragments arranged by size:
2468 1935 936 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2629 2819 4583 5967 6235 1632
Size: 997 190 1764 1384 268 1917
Fragments arranged by size:
1917 1764 1384 997 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 2712 2856 5419 6396 1203
Size: 1509 144 2563 977 1327
Fragments arranged by size:
2563 1509 1327 977 144
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 3092 1166
Size: 1926 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 3131 3163 2843
Size: 32 256 32 6200
Fragments arranged by size:
6200 256 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2356 2520 3292 3373 4263 5835 2356
Size: 164 772 81 890 1572 3041
Fragments arranged by size:
3041 1572 890 772 164 81
ApaI G_GGCC'C
Cuts at: 2069 2120 3443 2069
Size: 51 1323 5146
Fragments arranged by size:
5146 1323 51
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 3829 4055 4207 6316 1493
Size: 2336 226 152 2109 1697
Fragments arranged by size:
2336 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2221 2779 3232 3580 3764 3845 1870
Size: 73 278 558 453 348 184 81 4545
Fragments arranged by size:
4545 558 453 348 278 184 81 73
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2783 3511 3841 5296
Size: 325 217 627 441 555 728 330 1455
Cuts at: 5296 618
Size: 1842
Fragments arranged by size:
1842 1455 728 627 555 441 330 325
217
BanII G_rGCy'C
Cuts at: 1391 2069 2120 2172 2499 2664 3240 3321 3443
Size: 678 51 52 327 165 576 81 122
Cuts at: 3443 3780 3839 1391
Size: 337 59 4072
Fragments arranged by size:
4072 678 576 337 327 165 122 81
59 52 51
BbeI G_GCGC'C
Cuts at: 2787 2787
Size: 6520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2170 928
Size: 971 271 5278
Fragments arranged by size:
5278 971 271
BbvCI CC'TCA_GC
Cuts at: 2462 3536 3794 2462
Size: 1074 258 5188
Fragments arranged by size:
5188 1074 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2746 2780 4711 4745 1903
Size: 34 809 34 1931 34 3678
Fragments arranged by size:
3678 1931 809 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2334 2705 3273
Size: 107 122 71 631 605 662 371 568
Cuts at: 3273 5131 136
Size: 1858 1525
Fragments arranged by size:
1858 1525 662 631 605 568 371 122
107 71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 2171 2994 3937 2171
Size: 823 943 4754
Fragments arranged by size:
4754 943 823
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2120 2129 3443 3681 4581 5737 1790
Size: 279 51 9 1314 238 900 1156 2573
Fragments arranged by size:
2573 1314 1156 900 279 238 51 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 3527 3663
Size: 416 53 83 399 24 655 1455 136
Cuts at: 3663 4251 5209 442
Size: 588 958 1753
Fragments arranged by size:
1753 1455 958 655 588 416 399 136
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2173 2281 2363 2411 2507 2720 2870
Size: 860 74 108 82 48 96 213 150
Cuts at: 2870 2972 3038 3203 3335 3404 3506 3608 3623
Size: 102 66 165 132 69 102 102 15
Cuts at: 3623 5180 1239
Size: 1557 2579
Fragments arranged by size:
2579 1557 860 213 165 150 132 108
102 102 102 96 82 74 69 66
48 15
Bpu10I CC'TnA_GC
Cuts at: 2462 2678 3536 3794 2462
Size: 216 858 258 5188
Fragments arranged by size:
5188 858 258 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3044 3239 3620 4645 5510 5808 3044
Size: 195 381 1025 865 298 3756
Fragments arranged by size:
3756 1025 865 381 298 195
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5183 882
Size: 617 3684 2219
Fragments arranged by size:
3684 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4311 493
Size: 711 3107 2702
Fragments arranged by size:
3107 2702 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2784 3432 3468 4327
Size: 53 83 186 936 1251 648 36 859
Cuts at: 4327 4709 5344 275
Size: 382 635 1451
Fragments arranged by size:
1451 1251 936 859 648 635 382 186
83 53 36
BsaWI w'CCGG_w
Cuts at: 3126 3136 4953 5351 5625 5772 6183 3126
Size: 10 1817 398 274 147 411 3463
Fragments arranged by size:
3463 1817 411 398 274 147 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2843 2873 3131 3161 3314
Size: 30 165 30 1440 30 258 30 153
Cuts at: 3314 3344 3581 3611 1178
Size: 30 237 30 4087
Fragments arranged by size:
4087 1440 258 237 165 153 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 3296 1186
Size: 1783 327 4410
Fragments arranged by size:
4410 1783 327
BseYI C'CCAG_C
Cuts at: 2119 2332 2359 2872 2965 5723 6219 2119
Size: 213 27 513 93 2758 496 2420
Fragments arranged by size:
2758 2420 513 496 213 93 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2741 2927 3152 3161 1163
Size: 573 633 372 186 225 9 4522
Fragments arranged by size:
4522 633 573 372 225 186 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 3970 4731 5759 1540
Size: 275 186 132 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2172 2559 2664 3033 3321 4581 4666 5737 2172
Size: 387 105 369 288 1260 85 1071 2955
Fragments arranged by size:
2955 1260 1071 387 369 288 105 85
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
BsmBI CGTCTCn'nnnn_
Cuts at: 3475 3475
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2120 2129 2172 2499 2559 2664
Size: 399 279 51 9 43 327 60 105
Cuts at: 2664 3033 3240 3321 3443 3681 3780 3839 4581
Size: 369 207 81 122 238 99 59 742
Cuts at: 4581 4666 5737 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 327 279 238
207 122 105 99 85 81 60 59
51 43 9
BspEI T'CCGG_A
Cuts at: 3126 3136 6183 3126
Size: 10 3047 3463
Fragments arranged by size:
3463 3047 10
BspHI T'CATG_A
Cuts at: 3102 4409 6139 3102
Size: 1307 1730 3483
Fragments arranged by size:
3483 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2391 2637 2949 3133 3357 3399 3792
Size: 1481 66 246 312 184 224 42 393
Cuts at: 3792 3875 844
Size: 83 3489
Fragments arranged by size:
3489 1481 393 312 246 224 184 83
66 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 4407 1394
Size: 429 572 2012 3507
Fragments arranged by size:
3507 2012 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3491 5015 5189 65
Size: 1183 2243 1524 174 1396
Fragments arranged by size:
2243 1524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3224 3424 5164 5351 1700
Size: 110 1414 200 1740 187 2869
Fragments arranged by size:
2869 1740 1414 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 3269 4580 5592 3269
Size: 1311 1012 4197
Fragments arranged by size:
4197 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 4139
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 3608 1184
Size: 2424 4096
BstYI r'GATC_y
Cuts at: 1972 3850 4217 4602 4619 6071 6157 6169 6515
Size: 1878 367 385 17 1452 86 12 346
Cuts at: 6515 1972
Size: 1977
Fragments arranged by size:
1977 1878 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2012 3542 2012
Size: 1530 4990
BtgI C'CryG_G
Cuts at: 513 1065 2004 2575 2772 513
Size: 552 939 571 197 4261
Fragments arranged by size:
4261 939 571 552 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2749 2824 2893 487
Size: 683 56 1523 75 69 4114
Fragments arranged by size:
4114 1523 683 75 69 56
BtsI GCAGTG_nn'
Cuts at: 3554 4036 4830 4850 3554
Size: 482 794 20 5224
Fragments arranged by size:
5224 794 482 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2222 2257 529
Size: 35 1658 35 4792
Fragments arranged by size:
4792 1658 35 35
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2326 2655
Size: 54 1425 50 275 18 300 196 329
Cuts at: 2655 3967 4856 6485 8
Size: 1312 889 1629 43
Fragments arranged by size:
1629 1425 1312 889 329 300 275 196
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 3967 1537
Size: 275 318 1837 4090
Fragments arranged by size:
4090 1837 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 2648 3254 3368 4450 1353
Size: 1151 144 606 114 1082 3423
Fragments arranged by size:
3423 1151 1082 606 144 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3197 3230 3320 3443 5107 5481
Size: 1064 138 1608 33 90 123 1664 374
Cuts at: 5481 5627 387
Size: 146 1280
Fragments arranged by size:
1664 1608 1280 1064 374 146 138 123
90 33
EcoICRI GAG'CTC
Cuts at: 2170 2662 3319 2170
Size: 492 657 5371
Fragments arranged by size:
5371 657 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2173 2281 2363 2411 2507 2629
Size: 393 467 74 108 82 48 96 122
Cuts at: 2629 2720 2819 2870 2972 3038 3203 3335 3404
Size: 91 99 51 102 66 165 132 69
Cuts at: 3404 3506 3608 3623 4583 5180 5967 6235 1239
Size: 102 102 15 960 597 787 268 1524
Fragments arranged by size:
1524 960 787 597 467 393 268 165
132 122 108 102 102 102 99 96
91 82 74 69 66 51 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2849 3365 3797 2849
Size: 516 432 5572
Fragments arranged by size:
5572 516 432
EcoO109I rG'GnC_Cy
Cuts at: 2066 2116 2506 2524 2938 3010 3154 3370 3440
Size: 50 390 18 414 72 144 216 70
Cuts at: 3440 3790 2066
Size: 350 4796
Fragments arranged by size:
4796 414 390 350 216 144 72 70
50 18
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
FspI TGC'GCA
Cuts at: 2408 5026 2408
Size: 2618 3902
HaeII r_GCGC'y
Cuts at: 1678 2039 2357 2787 2807 3075 3595 3644 5667
Size: 361 318 430 20 268 520 49 2023
Cuts at: 5667 1678
Size: 2531
Fragments arranged by size:
2531 2023 520 430 361 318 268 49
20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3237 3269 3314 3346 5161
Size: 32 135 32 1665 32 45 32 1815
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1815 1665 135 45 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3864 4110 669
Size: 988 281 1926 246 3079
Fragments arranged by size:
3079 1926 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KasI G'GCGC_C
Cuts at: 2783 2783
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3357 5634 5818 1305
Size: 2052 2277 184 2007
Fragments arranged by size:
2277 2052 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4698
Fragments arranged by size:
4698 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 4478 4837 4996 518
Size: 648 1926 1386 359 159 2042
Fragments arranged by size:
2042 1926 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2404 2577 2649 2725 2965 2989 3192
Size: 726 72 173 72 76 240 24 203
Cuts at: 3192 3451 3936 4613 5761 6006 1606
Size: 259 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 485 259 245 240
203 173 76 72 72 24
NaeI GCC'GGC
Cuts at: 3426 3426
Size: 6520
NarI GG'CG_CC
Cuts at: 2784 2784
Size: 6520
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5029
Fragments arranged by size:
5029 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 3424 3424
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 3878 5423 1132
Size: 97 2649 1545 2229
Fragments arranged by size:
2649 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3436 3615 1348
Size: 2088 179 4253
Fragments arranged by size:
4253 2088 179
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3373 1108
Size: 2265 4255
PfoI T'CCnGG_A
Cuts at: 2189 2664 2847 2949 3054 2189
Size: 475 183 102 105 5655
Fragments arranged by size:
5655 475 183 105 102
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 3131 3163 4589 4621 6102 6134 2843
Size: 32 256 32 1426 32 1481 32 3229
Fragments arranged by size:
3229 1481 1426 256 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2938 3790 2524
Size: 414 852 5254
Fragments arranged by size:
5254 852 414
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2065 2116 3439 2065
Size: 51 1323 5146
Fragments arranged by size:
5146 1323 51
PstI C_TGCA'G
Cuts at: 830 2147 2186 2322 2634 2964 3450 3663 3872
Size: 1317 39 136 312 330 486 213 209
Cuts at: 3872 830
Size: 3478
Fragments arranged by size:
3478 1317 486 330 312 213 209 136
39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2332 2404 2725 2965 2989 3192 2332
Size: 72 321 240 24 203 5660
Fragments arranged by size:
5660 321 240 203 72 24
SacI G_AGCT'C
Cuts at: 2172 2664 3321 2172
Size: 492 657 5371
Fragments arranged by size:
5371 657 492
SacII CC_GC'GG
Cuts at: 2578 2578
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 2504 2648 2504
Size: 144 6376
SbfI CC_TGCA'GG
Cuts at: 2322 3872 2322
Size: 1550 4970
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2143 2182 2318 2630 2960 3446 3659
Size: 219 1098 39 136 312 330 486 213
Cuts at: 3659 3868 5003 5684 5875 826
Size: 209 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 681 486 330 312 219
213 209 191 136 39
SfoI GGC'GCC
Cuts at: 2785 2785
Size: 6520
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 2223 2781 3582 3847 1872
Size: 351 558 801 265 4545
Fragments arranged by size:
4545 801 558 351 265
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3023 3254 3635 4624 5525 5787
Size: 197 926 1080 231 381 989 901 262
Cuts at: 5787 820
Size: 1553
Fragments arranged by size:
1553 1080 989 926 901 381 262 231
197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 2025 1517
Size: 508 6012
StyI C'CwwG_G
Cuts at: 513 1065 2004 3959 513
Size: 552 939 1955 3074
Fragments arranged by size:
3074 1955 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2246 4547 4564 4717 4902 5347
Size: 1255 464 202 2301 17 153 185 445
Cuts at: 5347 325
Size: 1498
Fragments arranged by size:
2301 1498 1255 464 445 202 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4766 6478 96
Size: 274 80 33 51 1443 2789 1712 138
Fragments arranged by size:
2789 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 6480 6512 3581
Size: 32 2867 32 3589
Fragments arranged by size:
3589 2867 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 1316
Size: 900 5620
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3505 3505
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 2221 2779 3580 3845 1870
Size: 351 558 801 265 4545
Fragments arranged by size:
4545 801 558 351 265
XmnI GAAnn'nnTTC
Cuts at: 4649 4649
Size: 6520
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3469 4328 276
Size: 53 83 186 936 1935 859 2468
Fragments arranged by size:
2468 1935 936 859 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI