Restriction map for
pFN21AB7053
(Circular) (Six-base) MAPSORT of: pFN21AB7053.seq Check: 7129 from: 1 to: 4742
pFN21AB7053 4742 bp
With 182 enzymes: SgfI *
April 19, 2010 13:26 ..
AccI GT'mk_AC
Cuts at: 976 1780 976
Size: 804 3938
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1039 1221 1656 2567 2999 3746 4014 555
Size: 484 182 435 911 432 747 268 1283
Fragments arranged by size:
1283 911 747 484 435 432 268 182
AfeI AGC'GCT
Cuts at: 803 803
Size: 4742
AflIII A'CryG_T
Cuts at: 2094 3198 4175 2094
Size: 1104 977 2661
Fragments arranged by size:
2661 1104 977
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1243 1243
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 936 1017 2047 3614 936
Size: 81 1030 1567 2064
Fragments arranged by size:
2064 1567 1030 81
ApaI G_GGCC'C
Cuts at: 270 270
Size: 4742
ApaLI G'TGCA_C
Cuts at: 1564 3512 1564
Size: 1948 2794
ApoI r'AATT_y
Cuts at: 1730 1746 4095 4351 1730
Size: 16 2349 256 2121
Fragments arranged by size:
2349 2121 256 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 559 783 1762 559
Size: 224 979 3539
Fragments arranged by size:
3539 979 224
BamHI G'GATC_C
Cuts at: 169 1767 169
Size: 1598 3144
BanI G'GyrC_C
Cuts at: 256 723 907 1758 2172 2420 2455 256
Size: 467 184 851 414 248 35 2543
Fragments arranged by size:
2543 851 467 414 248 184 35
BanII G_rGCy'C
Cuts at: 243 270 566 670 790 1009 1172 1756 2786
Size: 27 296 104 120 219 163 584 1030
Cuts at: 2786 243
Size: 2199
Fragments arranged by size:
2199 1030 584 296 219 163 120 104
27
BbeI G_GCGC'C
Cuts at: 260 2176 2424 260
Size: 1916 248 2578
Fragments arranged by size:
2578 1916 248
BbvCI CC'TCA_GC
Cuts at: 624 1653 624
Size: 1029 3713
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3401 4742 2640
Size: 761 1341 2640
Fragments arranged by size:
2640 1341 761
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 265 1408 265
Size: 1143 3599
BglII A'GATC_T
Cuts at: 152 2257 4294 152
Size: 2105 2037 600
Fragments arranged by size:
2105 2037 600
BlpI GC'TnA_GC
Cuts at: 229 1854 229
Size: 1625 3117
Bme1580I G_kGCm'C
Cuts at: 270 910 1520 1568 2367 2460 3516 270
Size: 640 610 48 799 93 1056 1496
Fragments arranged by size:
1496 1056 799 640 610 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 240 1123 1954 2035 2370 4514 240
Size: 883 831 81 335 2144 468
Fragments arranged by size:
2144 883 831 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 544 620 790 958 232
Size: 312 76 170 168 4016
Fragments arranged by size:
4016 312 170 168 76
Bpu10I CC'TnA_GC
Cuts at: 206 624 676 761 1653 206
Size: 418 52 85 892 3295
Fragments arranged by size:
3295 892 418 85 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3289 3587 1918
Size: 879 492 298 3073
Fragments arranged by size:
3073 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 454 4446 454
Size: 3992 750
BsaAI yAC'GTr
Cuts at: 1601 2095 1601
Size: 494 4248
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 257 1099 2173 2421 3123 4619 257
Size: 842 1074 248 702 1496 380
Fragments arranged by size:
1496 1074 842 702 380 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3404 3551 3962 1931
Size: 521 678 274 147 411 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1698 1728 1698
Size: 30 4712
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 606 636 1407 1521 1533 1596 229
Size: 377 30 771 114 12 63 3375
Fragments arranged by size:
3375 771 377 114 63 30 12
BseYI C'CCAG_C
Cuts at: 472 950 1046 1060 1090 2167 2925 3502 3998
Size: 478 96 14 30 1077 758 577 496
Cuts at: 3998 472
Size: 1216
Fragments arranged by size:
1216 1077 758 577 496 478 96 30
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 515
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3538 104
Size: 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 404 1172 1568 1756 2534 2724 3516 404
Size: 768 396 188 778 190 792 1630
Fragments arranged by size:
1630 792 778 768 396 190 188
Bsp1286I G_dGCh'C
Cuts at: 243 270 404 566 670 790 910 1009 1172
Size: 27 134 162 104 120 120 99 163
Cuts at: 1172 1520 1568 1756 2367 2460 2534 2724 2786
Size: 348 48 188 611 93 74 190 62
Cuts at: 2786 3516 243
Size: 730 1469
Fragments arranged by size:
1469 730 611 348 190 188 163 162
134 120 120 104 99 93 74 62
48 27
BspEI T'CCGG_A
Cuts at: 3962 3962
Size: 4742
BspHI T'CATG_A
Cuts at: 3918 3918
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1792 2006 2308 2689 1792
Size: 214 302 381 3845
Fragments arranged by size:
3845 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1160 3034 3088 4572 4737 50
Size: 1110 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 1110 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 609 1085 2740 2921 3130 609
Size: 476 1655 181 209 2221
Fragments arranged by size:
2221 1655 476 209 181
BssSI C'ACGA_G
Cuts at: 3013 3371 3013
Size: 358 4384
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstYI r'GATC_y
Cuts at: 1 152 169 894 1355 1767 2234 2257 2592
Size: 151 17 725 461 412 467 23 335
Cuts at: 2592 2838 3850 3936 3948 4294 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 725 467 461 449 412 346 335
246 151 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 371 371
Size: 4742
BtgI C'CryG_G
Cuts at: 107 113 191 107
Size: 6 78 4658
Fragments arranged by size:
4658 78 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1346 2692 2873 3036 1346
Size: 1346 181 163 3052
Fragments arranged by size:
3052 1346 181 163
BtsI GCAGTG_nn'
Cuts at: 692 1985 692
Size: 1293 3449
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DraIII CAC_nnn'GTG
Cuts at: 554 554
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 414 2448 3306 414
Size: 2034 858 1850
Fragments arranged by size:
2034 1850 858
EaeI y'GGCC_r
Cuts at: 110 116 510 1409 1490 1592 2327 2501 2892
Size: 6 394 899 81 102 735 174 391
Cuts at: 2892 2919 4264 110
Size: 27 1345 588
Fragments arranged by size:
1345 899 735 588 394 391 174 102
81 27 6
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 574 1410 1431 2765 2975 574
Size: 836 21 1334 210 2341
Fragments arranged by size:
2341 1334 836 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 639 645 2950 3260 3406 4552 391
Size: 248 6 2305 310 146 1146 581
Fragments arranged by size:
2305 1146 581 310 248 146 6
EcoICRI GAG'CTC
Cuts at: 1170 1754 1170
Size: 584 4158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 544 555 620 790 958 1039 1221 1656
Size: 312 11 65 170 168 81 182 435
Cuts at: 1656 2567 2999 3746 4014 232
Size: 911 432 747 268 960
Fragments arranged by size:
960 911 747 435 432 312 268 182
170 168 81 65 11
EcoO109I rG'GnC_Cy
Cuts at: 266 267 933 1014 1023 1881 266
Size: 1 666 81 9 858 3127
Fragments arranged by size:
3127 858 666 81 9 1
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 861 1377 2761 861
Size: 516 1384 2842
Fragments arranged by size:
2842 1384 516
FspI TGC'GCA
Cuts at: 684 1072 1231 2523 684
Size: 388 159 1292 2903
Fragments arranged by size:
2903 1292 388 159
HaeII r_GCGC'y
Cuts at: 260 805 1679 2176 2424 3446 4425 260
Size: 545 874 497 248 1022 979 577
Fragments arranged by size:
1022 979 874 577 545 497 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2584 2614 2616 2646 2584
Size: 30 2 30 4680
Fragments arranged by size:
4680 30 30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 256 2172 2420 256
Size: 1916 248 2578
Fragments arranged by size:
2578 1916 248
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MfeI C'AATT_G
Cuts at: 1724 1724
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 1187 3413 3597 719
Size: 468 2226 184 1864
Fragments arranged by size:
2226 1864 468 184
MscI TGG'CCA
Cuts at: 1594 2503 1594
Size: 909 3833
MslI CAynn'nnrTG
Cuts at: 1225 2731 2858 1225
Size: 1506 127 3109
Fragments arranged by size:
3109 1506 127
MspA1I CmG'CkG
Cuts at: 115 135 147 281 308 640 721 1657 1853
Size: 20 12 134 27 332 81 936 196
Cuts at: 1853 2167 2527 3540 3785 115
Size: 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 936 360 332 314 245 196
134 81 27 20 12
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 4742
NarI GG'CG_CC
Cuts at: 257 2173 2421 257
Size: 1916 248 2578
Fragments arranged by size:
2578 1916 248
NcoI C'CATG_G
Cuts at: 107 191 107
Size: 84 4658
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 4742
NspI r_CATG'y
Cuts at: 1071 1230 1795 2295 3202 1071
Size: 159 565 500 907 2611
Fragments arranged by size:
2611 907 565 500 159
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PflMI CCAn_nnn'nTGG
Cuts at: 936 936
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1014 1014
Size: 4742
PshAI GACnn'nnGTC
Cuts at: 704 704
Size: 4742
PspOMI G'GGCC_C
Cuts at: 266 266
Size: 4742
PstI C_TGCA'G
Cuts at: 152 1789 2474 152
Size: 1637 685 2420
Fragments arranged by size:
2420 1637 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 135 1657 2167 2527 135
Size: 1522 510 360 2350
Fragments arranged by size:
2350 1522 510 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1172 1756 1172
Size: 584 4158
SacII CC_GC'GG
Cuts at: 116 116
Size: 4742
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 148 1785 2470 3463 3654 33
Size: 115 1637 685 993 191 1121
Fragments arranged by size:
1637 1121 993 685 191 115
SfiI GGCCn_nnn'nGGCC
Cuts at: 1408 1408
Size: 4742
SfoI GGC'GCC
Cuts at: 258 2174 2422 258
Size: 1916 248 2578
Fragments arranged by size:
2578 1916 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 785 1764 785
Size: 979 3763
SmlI C'TyrA_G
Cuts at: 1897 2812 3304 3566 1897
Size: 915 492 262 3073
Fragments arranged by size:
3073 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1071 1230 1795 2295 1071
Size: 159 565 500 3518
Fragments arranged by size:
3518 565 500 159
StuI AGG'CCT
Cuts at: 778 988 778
Size: 210 4532
StyI C'CwwG_G
Cuts at: 107 119 191 836 1876 2230 107
Size: 12 72 645 1040 354 2619
Fragments arranged by size:
2619 1040 645 354 72 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 549 741 964 1276 1288 2325 2458 2917 3126
Size: 192 223 312 12 1037 133 459 209
Cuts at: 3126 549
Size: 2165
Fragments arranged by size:
2165 1037 459 312 223 209 192 133
12
TatI w'GTAC_w
Cuts at: 4257 4257
Size: 4742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 716 748 4259 4291 716
Size: 32 3511 32 1167
Fragments arranged by size:
3511 1167 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 2237
Size: 4742
XmaI C'CCGG_G
Cuts at: 783 1762 783
Size: 979 3763
XmnI GAAnn'nnTTC
Cuts at: 925 1207 925
Size: 282 4460
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI