Restriction map for
pFN21AB7047
(Circular) (Six-base) MAPSORT of: pFN21AB7047.seq Check: 5197 from: 1 to: 6175
pFN21AB7047 6175 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2313 3518 1937
Size: 376 1205 4594
Fragments arranged by size:
4594 1205 376
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 3474 4302 4675 1563
Size: 1911 828 373 3063
Fragments arranged by size:
3063 1911 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2465 2697 2919 3089 3147 3339 4238
Size: 624 209 232 222 170 58 192 899
Cuts at: 4238 5622 5890 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 899 624 268 232 222 209
192 170 58
AfeI AGC'GCT
Cuts at: 3092 3092
Size: 6175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 3137 1166
Size: 1971 4204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2274 1184
Size: 1090 5085
AlwNI CAG_nnn'CTG
Cuts at: 3386 3918 5490 3386
Size: 532 1572 4071
Fragments arranged by size:
4071 1572 532
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2023 2132 2381 2516 2750 3484 3710 3862
Size: 530 109 249 135 234 734 226 152
Cuts at: 3862 5971 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 734 530 249 234 226 152
135 109
AseI AT'TA_AT
Cuts at: 160 1032 3272 4729 160
Size: 872 2240 1457 1606
Fragments arranged by size:
2240 1606 1457 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 1870
Size: 73 1557 4545
Fragments arranged by size:
4545 1557 73
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3450 3494 1391
Size: 2059 44 4072
Fragments arranged by size:
4072 2059 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 3135 928
Size: 971 192 1044 3968
Fragments arranged by size:
3968 1044 971 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BfrBI ATG'CAT
Cuts at: 2996 2996
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3292 6170 3292
Size: 2878 3297
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3338 3906 4864
Size: 416 53 83 399 24 1921 568 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
1921 1753 958 568 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3469 3467
Size: 2 6173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 3041 3418 4835 1239
Size: 812 990 377 1417 2579
Fragments arranged by size:
2579 1417 990 812 377
Bpu10I CC'TnA_GC
Cuts at: 3341 3341
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 2990 3413 4300 5165 5463 2080
Size: 910 423 887 865 298 2792
Fragments arranged by size:
2792 910 887 865 423 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5950
Fragments arranged by size:
5950 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2512 3440 1186
Size: 1326 928 3921
Fragments arranged by size:
3921 1326 928
BseYI C'CCAG_C
Cuts at: 5378 5874 5378
Size: 496 5679
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2763 1163
Size: 573 1027 4575
Fragments arranged by size:
4575 1027 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3450 4236 4321 5392 3450
Size: 786 85 1071 4233
Fragments arranged by size:
4233 1071 786 85
BsmI GAATG_Cn'
Cuts at: 2225 2998 3686 3779 2225
Size: 773 688 93 4621
Fragments arranged by size:
4621 773 688 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3017 3017
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3450 3494 4236 4321 5392 1391
Size: 399 1660 44 742 85 1071 2174
Fragments arranged by size:
2174 1660 1071 742 399 85 44
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 3530 844
Size: 1537 1149 3489
Fragments arranged by size:
3489 1537 1149
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4670 4844 65
Size: 1183 3422 174 1396
Fragments arranged by size:
3422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2163 96
Size: 2067 4108
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 2957 4979 2957
Size: 2022 4153
BstEII G'GTnAC_C
Cuts at: 1184 2014 2768 1184
Size: 830 754 4591
Fragments arranged by size:
4591 830 754
BstXI CCAn_nnnn'nTGG
Cuts at: 3255 3255
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2101 3292 3505 3872 4257 4274 5726 5812
Size: 129 1191 213 367 385 17 1452 86
Cuts at: 5812 5824 6170 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 213 129
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2555 487
Size: 683 56 1329 4107
Fragments arranged by size:
4107 1329 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3132 3691 4485 4505 2746
Size: 386 559 794 20 4416
Fragments arranged by size:
4416 794 559 386 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 2139 3479 2139
Size: 1340 4835
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140
Size: 54 1425 50 275 18 1792 889 1629
Cuts at: 6140 8
Size: 43
Fragments arranged by size:
1792 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 3186 4105 1353
Size: 879 954 919 3423
Fragments arranged by size:
3423 954 919 879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4762 5136 5282 387
Size: 1064 138 3173 374 146 1280
Fragments arranged by size:
3173 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3448 3448
Size: 6175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2256 2465 2697 2919 3041 3089
Size: 393 419 205 209 232 222 122 48
Cuts at: 3089 3147 3339 3418 4238 4835 5622 5890 1239
Size: 58 192 79 820 597 787 268 1524
Fragments arranged by size:
1524 820 787 597 419 393 268 232
222 209 205 192 122 79 58 48
EcoRI G'AATT_C
Cuts at: 2023 2516 3484 3862 2023
Size: 493 968 378 4336
Fragments arranged by size:
4336 968 493 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 3094 5322 1678
Size: 1416 2228 2531
Fragments arranged by size:
2531 2228 1416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2533 2565 3146
Size: 32 135 32 506 32 423 32 581
Cuts at: 3146 3178 4816 4848 4890 4922 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 581 506 423 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 2535 3774 2535
Size: 1239 4936
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 5289 5473 1305
Size: 1482 2502 184 2007
Fragments arranged by size:
2502 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 3137 4133 4492 4651 518
Size: 648 1027 944 996 359 159 2042
Fragments arranged by size:
2042 1027 996 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 3591 4268 5416 5661 1606
Size: 444 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 444 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5623
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NsiI A_TGCA'T
Cuts at: 2998 2998
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 2261 3533 5078 1132
Size: 97 1032 1272 1545 2229
Fragments arranged by size:
2229 1545 1272 1032 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2825 1108
Size: 1717 4458
PfoI T'CCnGG_A
Cuts at: 3018 3018
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2590 4244 4276 5757 5789 2558
Size: 32 1654 32 1481 32 2944
Fragments arranged by size:
2944 1654 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2658 3527 830
Size: 1828 869 3478
Fragments arranged by size:
3478 1828 869
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 6175
SacI G_AGCT'C
Cuts at: 3450 3450
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2654 3523 4658 5339 5530 826
Size: 219 1609 869 1135 681 191 1471
Fragments arranged by size:
1609 1471 1135 869 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 2969 3392 4279 5180 5442
Size: 197 926 152 874 423 887 901 262
Cuts at: 5442 820
Size: 1553
Fragments arranged by size:
1553 926 901 887 874 423 262 197
152
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2703 3614 513
Size: 552 1638 911 3074
Fragments arranged by size:
3074 1638 911 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2577 2838 4202 4219 4372 4557 5002
Size: 1255 997 261 1364 17 153 185 445
Cuts at: 5002 325
Size: 1498
Fragments arranged by size:
1498 1364 1255 997 445 261 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 4421 6133
Size: 274 80 33 51 1443 186 2258 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
2258 1712 1443 274 186 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 2090 1316
Size: 774 5401
XbaI T'CTAG_A
Cuts at: 2104 3511 2104
Size: 1407 4768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2026
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 2075 2573 2793 4304 2075
Size: 498 220 1511 3946
Fragments arranged by size:
3946 1511 498 220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI