Restriction map for
pFN21AB7044
(Circular) (Six-base) MAPSORT of: pFN21AB7044.seq Check: 804 from: 1 to: 6121
pFN21AB7044 6121 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2206 2174
Size: 32 6089
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3105 3442 3105
Size: 337 5784
AclI AA'CG_TT
Cuts at: 1563 3048 4248 4621 1563
Size: 1485 1200 373 3063
Fragments arranged by size:
3063 1485 1200 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 2497 2716 3244 4184 5568 5836 1632
Size: 855 10 219 528 940 1384 268 1917
Fragments arranged by size:
1917 1384 940 855 528 268 219 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 3018 5020 5997 1203
Size: 1815 2002 977 1327
Fragments arranged by size:
2002 1815 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 2488 3379 4850 2488
Size: 891 1471 3759
Fragments arranged by size:
3759 1471 891
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2909 3864 5436 2909
Size: 955 1572 3594
Fragments arranged by size:
3594 1572 955
ApaI G_GGCC'C
Cuts at: 2295 2708 2295
Size: 413 5708
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 3430 3656 3808 5917 1493
Size: 1937 226 152 2109 1697
Fragments arranged by size:
2109 1937 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 2645 2710 3446 1870
Size: 73 483 219 65 736 4545
Fragments arranged by size:
4545 736 483 219 73 65
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3089 3122 3089
Size: 33 6088
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 3105 3442 4897 618
Size: 325 217 627 278 1040 337 1455 1842
Fragments arranged by size:
1842 1455 1040 627 337 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2295 2434 2588 2708 2811 2963 3152 3440
Size: 904 139 154 120 103 152 189 288
Cuts at: 3440 1391
Size: 4072
Fragments arranged by size:
4072 904 288 189 154 152 139 120
103
BbeI G_GCGC'C
Cuts at: 2069 2069
Size: 6121
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 2970 3250 3303 928
Size: 971 604 467 280 53 3746
Fragments arranged by size:
3746 971 604 467 280 53
BbvCI CC'TCA_GC
Cuts at: 3119 3212 3119
Size: 93 6028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2609 4013 5223 2609
Size: 1404 1210 3507
Fragments arranged by size:
3507 1404 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2384 4732 136
Size: 107 122 71 631 605 712 2348 1525
Fragments arranged by size:
2348 1525 712 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2295 2708 2940 4182 5338 1790
Size: 505 413 232 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 505 413 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3051 3214 3234
Size: 416 53 83 399 24 1634 163 20
Cuts at: 3234 3852 4810 442
Size: 618 958 1753
Fragments arranged by size:
1753 1634 958 618 416 399 163 83
53 24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4781 1239
Size: 3542 2579
Bpu10I CC'TnA_GC
Cuts at: 3119 3212 3119
Size: 93 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2801 2825 2977 4246 5111 5409 2111
Size: 690 24 152 1269 865 298 2823
Fragments arranged by size:
2823 1269 865 690 298 152 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 3311 6115 3311
Size: 2804 3317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2112 2310 3928
Size: 53 83 186 936 533 46 198 1618
Cuts at: 3928 4310 4945 275
Size: 382 635 1451
Fragments arranged by size:
1618 1451 936 635 533 382 198 186
83 53 46
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2551 3379 1186
Size: 1365 828 3928
Fragments arranged by size:
3928 1365 828
BseYI C'CCAG_C
Cuts at: 3068 3093 5324 5820 3068
Size: 25 2231 496 3369
Fragments arranged by size:
3369 2231 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 1163
Size: 573 410 5138
Fragments arranged by size:
5138 573 410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2394 3571 4332 5360 1540
Size: 275 186 393 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 393 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 2963 4182 4267 5338 2811
Size: 152 1219 85 1071 3594
Fragments arranged by size:
3594 1219 1071 152 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
BsmBI CGTCTCn'nnnn_
Cuts at: 2475 2475
Size: 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2434 2588 2708 2811 2940 2963
Size: 399 505 139 154 120 103 129 23
Cuts at: 2963 3152 3440 4182 4267 5338 1391
Size: 189 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 288 189 154
139 129 120 103 85 23
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2206 3476 844
Size: 1330 32 1270 3489
Fragments arranged by size:
3489 1330 1270 32
BsrBI CCG'CTC
Cuts at: 1394 1823 2194 4008 1394
Size: 429 371 1814 3507
Fragments arranged by size:
3507 1814 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2229 4765 4952 1700
Size: 110 419 2536 187 2869
Fragments arranged by size:
2869 2536 419 187 110
BsrGI T'GTAC_A
Cuts at: 96 2031 96
Size: 1935 4186
BssHII G'CGCG_C
Cuts at: 1925 2091 2093 2278 1925
Size: 166 2 185 5768
Fragments arranged by size:
5768 185 166 2
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstXI CCAn_nnnn'nTGG
Cuts at: 3064 3064
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116
Size: 1479 367 385 17 1452 86 12 346
Cuts at: 6116 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2060 2107 2388 2601 2847 3291 513
Size: 552 995 47 281 213 246 444 3343
Fragments arranged by size:
3343 995 552 444 281 246 213 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2806 2841 529
Size: 35 2242 35 3809
Fragments arranged by size:
3809 2242 35 35
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DraIII CAC_nnn'GTG
Cuts at: 3177 3177
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2391 3568
Size: 54 1425 50 275 18 555 6 1177
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1177 889 555 275 54 50
43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2391 3568 1537
Size: 275 579 1177 4090
Fragments arranged by size:
4090 1177 579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4051 1353
Size: 2698 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2809 2961 2809
Size: 152 5969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2497 2716 3244 4184 4781 5568
Size: 393 855 10 219 528 940 597 787
Cuts at: 5568 5836 1239
Size: 268 1524
Fragments arranged by size:
1524 940 855 787 597 528 393 268
219 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2699 2699
Size: 6121
EcoO109I rG'GnC_Cy
Cuts at: 2291 2292 2704 2705 2833 3216 2291
Size: 1 412 1 128 383 5196
Fragments arranged by size:
5196 412 383 128 1 1
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2492 2587 2492
Size: 95 6026
FspI TGC'GCA
Cuts at: 2214 4627 2214
Size: 2413 3708
HaeII r_GCGC'y
Cuts at: 1678 2069 2225 2847 5268 1678
Size: 391 156 622 2421 2531
Fragments arranged by size:
2531 2421 622 391 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2582 2607 2614
Size: 32 135 32 902 32 76 25 7
Cuts at: 2614 2639 2707 2739 2862 2894 3365 3397 4762
Size: 25 68 32 123 32 471 32 1365
Cuts at: 4762 4794 4836 4868 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 902 471 135 123 76 68
42 32 32 32 32 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KasI G'GCGC_C
Cuts at: 2065 2065
Size: 6121
KpnI G_GTAC'C
Cuts at: 3109 3446 3109
Size: 337 5784
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2387 5235 5419 1305
Size: 1082 2848 184 2007
Fragments arranged by size:
2848 2007 1082 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2390 3112 3211 3537 4214 5362 5607
Size: 594 190 722 99 326 677 1148 245
Cuts at: 5607 1606
Size: 2120
Fragments arranged by size:
2120 1148 722 677 594 326 245 190
99
NaeI GCC'GGC
Cuts at: 2231 2231
Size: 6121
NarI GG'CG_CC
Cuts at: 2066 2066
Size: 6121
NcoI C'CATG_G
Cuts at: 513 1065 2107 513
Size: 552 1042 4527
Fragments arranged by size:
4527 1042 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NgoMIV G'CCGG_C
Cuts at: 2229 2229
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 2391 3568 2391
Size: 1177 4944
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 3216 2833
Size: 383 5738
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PspOMI G'GGCC_C
Cuts at: 2291 2704 2291
Size: 413 5708
PstI C_TGCA'G
Cuts at: 830 2076 2439 3081 3198 3473 830
Size: 1246 363 642 117 275 3478
Fragments arranged by size:
3478 1246 642 363 275 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 3211 3211
Size: 6121
SacI G_AGCT'C
Cuts at: 2811 2963 2811
Size: 152 5969
SacII CC_GC'GG
Cuts at: 2391 2391
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SexAI A'CCwGG_T
Cuts at: 2835 2835
Size: 6121
SfcI C'TryA_G
Cuts at: 826 1045 2072 2435 3077 3194 3469 4604 5285
Size: 219 1027 363 642 117 275 1135 681
Cuts at: 5285 5476 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1027 681 642 363 275 219
191 117
SfoI GGC'GCC
Cuts at: 2067 2067
Size: 6121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 2428 3448 1872
Size: 556 1020 4545
Fragments arranged by size:
4545 1020 556
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2804 2816 2956 4225 5126
Size: 197 926 183 678 12 140 1269 901
Cuts at: 5126 5388 820
Size: 262 1553
Fragments arranged by size:
1553 1269 926 901 678 262 197 183
140 12
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2107 3063 3560 513
Size: 552 1042 956 497 3074
Fragments arranged by size:
3074 1042 956 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3315 4148 4165 4318 4503 4948 325
Size: 1255 1735 833 17 153 185 445 1498
Fragments arranged by size:
1735 1498 1255 833 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 4367 6079
Size: 274 80 33 51 1443 54 2336 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
2336 1712 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6081 6113 6081
Size: 32 6089
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 1316
Size: 996 5125
XbaI T'CTAG_A
Cuts at: 3398 3457 3398
Size: 59 6062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2989 2248
Size: 741 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 2426 3446 1870
Size: 556 1020 4545
Fragments arranged by size:
4545 1020 556
XmnI GAAnn'nnTTC
Cuts at: 2856 4250 2856
Size: 1394 4727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI